SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene511

Length: 1564 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene511 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative alpha/beta hydrolase [Arabidopsis thaliana]	-	-	51%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Blast2go	alpha beta fold family expressed	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	70%
Blast2go	hydrolase activity	GO:0016787	Molecular Function	70%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	70%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Alpha/beta hydrolase fold-1	IPR000073	-	-
Sma3	Epoxide hydrolase-like	IPR000639	-	-
Sma3	Ion transport 2	IPR013099	-	-
Sma3	Isocitrate lyase/phosphorylmutase, conserved site	IPR018523	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AB65

Fln msg: your sequence is longer than subject: 398 - 363

Fln protein: MLADPDSLFVDVNGVQVHHKLADFSTEISIVGTEDMGNALSEITKQSNPPIGVPAILLHGFGASVFSWNQVLKPLAKVVGSKVVAFDRPAFGLSSRIQLAGSASDKNGKLPTMNPYSLGFSVAATLSFMDLLQSQKAILIGHSAGCLVAANAYFEAPERVAALILVAPAIVAPLTIGKNLNSNENARQRNENENSSSKDEGNPFRKIRSVLFRFWMGVSRLITNMMQEMKAMVDSFYRKTVSAIFRSHLAVMLVRFVMDKYSREAVRYAWYDSQKVTDHVIQGYTKPLKCKGWERALLDFTLAMITDSASEGKPPLKKRLKDISCPVLVVTGDTDRLVPAWNAERLAKAIPGSKLEVIKNCGHLPQEEKPEEFLAIVQKFLQWVVSTSNQPVLQAAI*

Protein Length: 399

Fln nts: GAAGTTAATAATAGCTGGAGTAGATCAGGATGAGTTACTGGATCCCCTG_-_ATGTTAGCTGATCCAGATAGTCTTTTTGTGGACGTCAACGGGGTTCAAGTCCACCATAAATTAGCGGATTTTTCTACTGAGATTTCTATTGTGGGCACAGAGGACATGGGCAATGCGCTTTCTGAAATTACCAAACAGAGTAACCCTCCAATTGGGGTTCCTGCAATTCTGTTGCACGGGTTTGGAGCATCCGTATTCTCATGGAACCAAGTGCTTAAACCACTGGCTAAGGTAGTTGGTTCGAAAGTGGTGGCTTTTGATAGGCCTGCATTTGGGCTGAGTTCCAGAATCCAGCTCGCAGGCTCTGCTTCTGATAAGAATGGGAAATTGCCCACTATGAACCCTTATTCGCTCGGCTTTTCAGTTGCGGCAACTTTGAGTTTTATGGACCTTTTGCAGTCCCAGAAAGCCATATTAATTGGGCATTCTGCAGGCTGTCTAGTAGCAGCAAATGCATACTTCGAAGCTCCTGAACGGGTTGCAGCACTGATTCTAGTAGCACCTGCGATTGTAGCTCCTTTGACCATTGGGAAGAATTTGAATTCAAATGAAAATGCAAGGCAAAGGAATGAAAATGAGAATTCGAGTTCAAAAGATGAAGGAAATCCATTTAGGAAAATCAGATCGGTGCTTTTCAGGTTCTGGATGGGTGTTTCTAGACTAATTACAAACATGATGCAAGAAATGAAAGCAATGGTAGATAGTTTTTACAGGAAAACTGTGTCTGCAATCTTCCGTTCTCACTTGGCAGTAATGTTGGTTAGATTTGTTATGGATAAATATAGCAGAGAAGCAGTTAGGTATGCATGGTATGATAGTCAGAAGGTAACTGACCATGTCATACAAGGATACACAAAGCCACTGAAATGCAAGGGTTGGGAGAGGGCTCTCCTTGACTTCACATTAGCCATGATTACTGATTCTGCATCTGAAGGAAAACCTCCACTCAAAAAACGGCTTAAAGACATCTCATGCCCTGTGTTGGTTGTGACCGGTGACACGGATCGGTTGGTTCCTGCATGGAACGCTGAGCGCCTAGCAAAGGCTATACCTGGATCCAAACTCGAAGTTATCAAAAATTGCGGTCACTTACCCCAGGAAGAGAAGCCAGAGGAATTCCTGGCTATAGTTCAGAAATTCTTGCAATGGGTTGTCAGCACTTCTAACCAACCGGTTCTTCAAGCAGCCATATAA___AATCCTATGTCACATCTTTGAATCACTGTATCTAAGTGATAGGTTGGTATTGTGCAATAAGCCTTCTTGAGTAAGTTCCTGAAGAATGTATTTTTAAAATGGATCCGAGGGAATAGTAACTGCAAAAAGGAATTAGATAGTGGTTTTCATGTAGCAACCATACACAAGAAAGAGTGACAGTGGCAAAATATGCCTGTCAGATAAATTGAGAATTGCATGTTATGTTCTTTGATAAGTTGAATTGTGAGTAGCCATGCAATTCAAATAAAAAGAGAGGAAAATGCTGAATTAATAATATCTCCTTCAATTCTGCGGCAGCTT

Fln Alignment:

Contig511___MGNALSEITKQSNPPIGVPAILLHGFGASVFSWNQVLKPLAKVVGSKVVAFDRPAFGLSSRIQLAGSASDKNGKLPTMNPYSLGFSVAATLSFMDLLQSQKAILIGHSAGCLVAANAYFEAPERXXXXXXXXXXXXXXLTIGKNLXXXXXXXXXXXXXXXXS-KDEGNPFRKIRSVLFRFWMGVSRLITNMMQEMKAMVDSFYRKTVSAIFRSHLAVMLVRFVMDKYSREAVRYAWYDSQKVTDHVIQGYTKPLKCKGWERALLDFTLAMITDSASEGKPPLKKRLKDISCPVLVVTGDTDRLVPAWNAERLAKAIPGSKLEVIKNCGHLPQEEKPEEFLAIVQKFLQWVVSTSNQPVLQAAI
D5AB65_____________MGNGLSEISNQSNPPIGVPAILLHGFGASAFSWNQVLKSLAKVLGSKVTAFDRPAFGLSSRIQLPASASDKNEKFPTMNPYSLGFSVAATLRFIDLLQSQKAILIGHSAGCLIAANAYFEAPERVAALILVAPAIVAPLAIGKNLNSNENVRKRNKLYENYSPKDEENPFRKIRRTLFRFWMGVSRLITSMLQEMKAMADNFYRKILSAIFRSSLAVMLVRLIMDKYSREAVRYAWYDSQKVTDHVIQGYTKPLKCKGWERALLEFTLATITDSASEGKPPLKKRLKDISCPVLVVTGDTDRLVPAWNAERLAKAIPGSKFEVIKNCGHLPQEERPEEFLAIVQKFLQWAVSNSNQPVLQAAI

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
257	87	12			0.992554
305	87			12	0.992554
376	11		88		0.923564
400	12			87	0.992554
432	14	85			0.993533
435	85	14			0.993533
437	14			85	0.993533
438			14	85	0.993533
439			14	85	0.993533
441		14		85	0.993533
444		14		85	0.993533
445	85		14		0.993533
448			14	85	0.993533
453	16		83		0.994512
515		12	87		0.992554
516		12		87	0.992554
517		12	87		0.992554
644		12		87	0.992554
645		12		87	0.992554
683		11		88	0.923564
785	87		12		0.992554
800	16			83	0.994512
803	20	80			0.995493
805			80	20	0.995493
838	20	80			0.995493
841		80	20		0.995493
860	25		75		0.996473
862	75		25		0.996473
1237		90		10	0.90298
1264	12	87			0.992554
1266		87		12	0.992554
1267		87	12		0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain