SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene462

Length: 1294 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene462 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam08755, YccV-like, Hemimethylated DNA-binding protein YccV like	-	-	5.0e-30	42%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	94%
Blast2go	uvrb uvrc motif-containing protein	-	-	0.0	81%

Source	GOs	Term	Type	Identity
Blast2go	nucleotide-excision repair	GO:0006289	Biological Process	81%
Blast2go	DNA binding	GO:0003677	Molecular Function	81%
Blast2go	nuclease activity	GO:0004518	Molecular Function	81%
Blast2go	chloroplast	GO:0009507	Cellular Component	81%
Blast2go	mitochondrion	GO:0005739	Cellular Component	81%

Source	InterPros	Term	Type	e value	Identity
Sma3	Hemimethylated DNA-binding domain	IPR011722	-	0.0	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKM7

Fln msg: your sequence is shorter than subject: 279 - 340

Fln protein: GRCGLRVIAAGMSKGNDPTSTAISECSESANEDILLFFFQLDLTTRIQCALNTDQYEIAQQLRSKLSEVEQEVARQRESKMGSTSSKDEAQDKAIAILRLRTDLQKAIEDEDYVYAAELRNKISKLEAESLAAAAKALAYQNVEYAFRLGQKVRHAVFGYRAVICGMDPLCCESSSWIENANVDKLSRGRNQPFYQVLVDVHADPSPLVAYVAEENLLKLDEPDLDRFDHPYIFLLFYGMDAAGDFIPIKQLREKYNRLRHEVPGEYDGNDVDSNKGT*

Protein Length: 280

Fln nts: ATGGGAGGTGTGGCTTGAGGGTCATAGCTGCTGGGATGTCCAAAGGCAATGATCCCACATCAACTGCTATTTCAGAATGCAGTGAGAGTGCTAATGAGGACATTTTGCTCTTCTTTTTCCAGCTAGATTTAACTACTCGCATTCAGTGTGCTTTAAACACAGATCAATATGAAATAGCTCAACAATTACGGAGCAAGCTTTCAGAGGTTGAGCAAGAGGTTGCTAGACAAAGGGAATCAAAGATGGGATCAACATCATCCAAGGATGAAGCTCAAGACAAAGCAATAGCGATTTTGCGTCTACGAACTGATCTGCAAAAAGCTATTGAGGATGAGGATTATGTCTATGCTGCTGAGTTACGCAATAAAATTTCCAAGCTCGAGGCAGAGTCTTTGGCAGCAGCGGCCAAGGCATTGGCTTACCAGAATGTAGAGTATGCATTTCGACTTGGGCAGAAAGTAAGGCACGCAGTTTTTGGTTATCGTGCTGTAATATGTGGAATGGATCCTTTATGCTGTGAGTCAAGTTCTTGGATTGAGAATGCGAATGTTGACAAATTGTCTAGAGGACGTAATCAACCTTTCTATCAGGTTTTGGTGGACGTACATGCAGATCCATCTCCACTGGTGGCTTATGTTGCTGAGGAGAACCTGTTAAAACTTGACGAACCAGATTTGGACCGATTTGATCATCCTTACATATTTCTCTTGTTTTATGGAATGGATGCTGCTGGAGACTTCATCCCCATAAAACAGTTGCGTGAGAAGTATAACAGGCTACGACATGAAGTGCCTGGGGAGTATGATGGTAATGATGTCGATAGTAACAAAGGTACCTAA___TAGTTGGGCAGATTACATGCAAAAGGAGCAGGTTTTAGTTAGGTTTGCAAAATGATTGCAGACCTCTCAAGTTATATGCTTACTAGCTTGCTTACACATTAATCTCCTCTGCATGATGGAACCTACTACCTACCAGGATACCTGTATATTCAACTATATTGAGCCCGGCGAGCCTGTTTCTATCCATCCAGTTGCTTGTAAATATTTATGGGAAGCAGTGCCCAGAACTCTATTGATATTTTAAAAATGTTAAAATTATTCTTGAGATATGTTACGTTTTACCACCCAACAATATTCATGATATGTTCATGAATGTATGCTCATTCCGAAATATCAGACAGGAAACTTGACTGTCTTTTGAATTGGTTTTTATGTCAACGAGGGACCGAAAGAAAATATAAAACACTTCTGAATGCATATGCTAGAAAATATTAAAATTTATTTGCATAGTCACC

Fln Alignment:

Contig462___RCGLRVIAAGMSKGNDPTSTAISECSESANEDILLFFFQLDLTTRIQCALNTDQYEIAQQLRSKLSEVEQEVARQRESKMGSTSSKDEAQDKAIAILRLRTDLQKAIEDEDYVYAAELRNKIXXXXXXXXXXXXXXXAYQNVEYAFRLGQKVRHAVFGYRAVICGMDPLCCESSSWIENANVDKLSRGRNQPFYQVLVDVHADPSPLVAYVAEENLLKLDEPDLDRFDHPYIFLLFYGMDAAGDFIPIKQLREKYNRLRHEVPGEYD-GNDVDSNKGT
B8LKM7_____________RRGLRVIAAGMSKGNDPTVTAISECSESANEDILLFFFQLDLTTRIQCALNSDQYEIAQQLRSKLAEVEQEVARQRESKMGSTSSKDEAQDKAIAILRLRTDLQKAIEDEDYAYAAELRNKISKLEADSLAAAAKALAYQNAEYAFRLGQKVRHAIFGYRAVICGMDPLCCESSSWIENANVDKLSRGRNQPFYQVLVDVHADPSPLVAYVAEENLLKLDEPDLDRFDHPYIFLLFYGMDTAGDFIPIKQLREKYNRQRHEVHGQYDNGNDVDNNKGT

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
673		7		92	0.990109
717		11	88		0.923564
718	22		77		0.999999
719			10	90	0.90298
723	10			90	0.90298
724			10	90	0.90298
725	90	10			0.90298
727	30		70		1.0
728	10			90	0.90298
729		90	10		0.90298
730		90	10		0.90298
733	10			90	0.90298
734	90	10			0.90298
738	88		11		0.923564
746	62	37			1.0
785		14		85	0.993533
917	33		66		1.0
1028		66		33	0.997452
1096	45	54			1.0
1150	90	10			0.90298
1153	10		90		0.90298
1154	90	10			0.90298
1161			88	11	0.923564
1274		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain