SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene459

Length: 1846 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene459 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative protein kinase 2 [Oryza sativa Japonica Group]	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Blast2go	probable receptor-like protein kinase at1g80640-like	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	78%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	78%
Blast2go	signal transducer activity	GO:0004871	Molecular Function	78%
Blast2go	ATP binding	GO:0005524	Molecular Function	78%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	78%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Uncharacterised protein family UPF0197	IPR007915	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	ABC transporter, conserved site	IPR017871	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AA69

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 507 - 587

Fln protein: GQSGDRSRKM*YGESMRLV*QKLYTVSMPLAPPGGAHS*WSAVSVGNPSGAQSGRRRDCRDWNTLQEWRA*PIPGQATSLQQTKPLKSTRHEIQHLKVLIIVAIALVLLILGAIIILFTYWTCTWVSRSRLCPVLPSSQPPALRKQSDIDDSDANNGMSLGPLLTRFSSFRTGKRKGCASAIEYSVLQAATNNFSSYNLLGQGGFGSVYRAQFPDDFCVAVKMLDENRKQADDEFQSEVELMSKIRHPNLVSLLGFCVHGKRRLLVYELMQNGSLEAHLHGPSHGSALTWHLRMKIALDTARGLEHLHDHCNSSIIHSDFKSANILLDASFNAKLSDFGLAVRAGGHMCSTDAELLGTLGYLAPEYLSDGKLTEKSDVYAFGVVLLELITGRKPVDKSMPAGCQSLVTWAKPQLTDRSKLPTIVDPMMKETVHLKHLYQVAAVAVVCVQTEPDYRPLITDVVHSLMPLVPVELGGALKNAKALSDNETKSLVAANLSTKTTGKTEL*

Protein Length: 508

Fln nts: ATGGGCAGTCTGGAGATAGAAGTAGAAAGATGTAGTACGGTGAATCAATGCGATTGGTATAACAAAAGCTTTACACTGTATCAATGCCATTGGCACCACCCGGCGGAGCACACTCTTGATGGAGTGCAGTATCCGTTGGAAATCCATCTGGTGCACAAAGCGGACGACGGCGAGATTGCCGTGATTGGAATACTTTACAAGAGTGGCGCGCATGACCCATTCCTGGACAAGCAACATCCCTACAGCAAACAAAGCCCCTGAAGTCCACAAGACATGAAATTCAACATCTCAAAGTTCTGATCATTGTGGCCATTGCTCTTGTGCTCCTCATTTTAGGGGCCATCATCATCCTTTTCACATATTGGACTTGTACATGGGTGTCCAGGAGTCGTTTATGTCCAGTTCTTCCATCATCTCAACCTCCAGCATTACGAAAACAGTCAGATATTGATGACTCAGATGCCAATAATGGAATGTCACTTGGCCCCTTGCTTACTCGTTTCAGCTCTTTCAGAACTGGTAAAAGGAAGGGATGCGCATCTGCTATAGAGTATTCTGTTTTACAAGCAGCTACAAATAATTTTAGTAGCTATAACCTCCTTGGGCAGGGAGGCTTTGGTTCTGTATACAGAGCTCAGTTCCCTGATGATTTTTGTGTTGCGGTCAAGATGCTAGATGAAAACAGAAAACAGGCAGACGATGAATTTCAGAGTGAAGTTGAACTAATGAGCAAAATCAGGCATCCAAACCTCGTGTCTTTGTTGGGTTTCTGTGTCCATGGGAAGAGAAGGCTGCTAGTATACGAGCTTATGCAAAACGGTTCTTTGGAAGCACACTTACACGGTCCATCTCATGGATCTGCACTTACATGGCATCTGCGTATGAAGATTGCTCTTGATACGGCCAGGGGTCTAGAGCATCTGCATGATCACTGCAATTCATCAATAATTCATAGTGATTTTAAATCAGCTAATATCCTTCTGGATGCAAGTTTCAATGCCAAACTTTCAGATTTTGGCCTAGCAGTAAGAGCTGGGGGACATATGTGCAGTACAGATGCTGAGCTATTAGGAACCCTGGGATATCTAGCTCCAGAGTACCTTTCTGATGGGAAACTGACGGAGAAAAGTGATGTTTATGCATTTGGAGTTGTTCTATTGGAGTTGATTACTGGCAGAAAACCTGTTGACAAATCTATGCCAGCTGGTTGCCAATCTCTTGTTACATGGGCCAAGCCACAGTTGACAGATAGGTCAAAGCTTCCAACAATTGTGGATCCTATGATGAAAGAGACTGTGCATTTGAAACACCTATATCAAGTGGCAGCTGTTGCTGTTGTTTGTGTGCAAACAGAGCCTGATTATAGACCACTTATAACTGATGTTGTACATTCTCTTATGCCTCTTGTACCGGTAGAGCTTGGAGGGGCATTAAAGAACGCAAAAGCACTATCTGACAATGAGACGAAATCACTGGTTGCTGCTAATTTAAGTACCAAAACAACCGGGAAGACGGAACTATAA___GCAGATATTAGTGCCCATTGGGATGGGAATGTATATTTTTCTTAATATTGTGTTTGTACGGTGACTTTATATTATATTAAGTGCTGCTTAAGTACTAGTACATTTATACCAAATTTTTCTTGGCAACTTTTAAGGTTAAGATGGAAGTTACTATTGATTGAAATTTTGTTTCGGTTGACGATTTGTCAACTGGAATTCAAGCAGCAATGTTTGTAGATTTTCTTTGTTGAAAAAGGAAAAGGAAATTCAAAATAGTGTATTAAATTTTCCTTTTTAAGGGAAATGCTTGCCATTCAGAAAATAAAACATTGAAGATTAAGTAC

Fln Alignment:

Contig459___PIPGQATSLQQTKPLKSTRHEIQHXXXXXXXXXXXXXXXXXXXXXLFTYWTCTWVSRSRLCPVLPSSQPPALRKQSDIDDSDANNGMSLGPLLTRFSSFRTGKRKGCASAIEYSVLQAATNNFSSYNLLGQGGFGSVYRAQFPDDFCVAVKMLDENRKQADDEFQSEVELMSKIRHPNLVSLLGFCVHGKRRLLVYELMQNGSLEAHLHGPSHGSALTWHLRMKIALDTARGLEHLHDHCNSSIIHSDFKSANILLDASFNAKLSDFGLAVRAGGHMCSTDAELLGTLGYLAPEYLSDGKLTEKSDVYAFGVVLLELITGRKPVDKSMPAGCQSLVTWAKPQLTDRSKLPTIVDPMMKETVHLKHLYQXXXXXXXXVQTEPDYRPLITDVVHSLMPLVPVELGGALKNAKALSDNETKSLVAANLSTKTTGKTEL*ADI
D5AA69_____________PMASMSTSLQQAKSLKSTKQEMQHLKVLSLVAIPITLLILGAILL-FTYYT--WVSRSRLCPVLPPSQPQALRKQSDNDDSDANNGMSIGPFLTRLSSFRTGKRKGCASAIEYSVLQAATNNFSSYNFLGKGGFGSVYRAQFHDDFCVAVKMLDENRKQADNEFQSEVELMSKIRHPNLVSLLGFCVHGKTRLLVYELMQNGSLEEHLHGPSHGAALTWHLRMKIALDTARGLEHLHEHCNSSIIHSDFKSANILLDASFNAKLSDFGLAVRAGGCICSTDVELLGTLGYLAPEYLFDGKLTEKSDVYAFGVVLLELITGRKPVDKSMPAGCQSLVTWAKPQLTDRSKLPTIVDPMIKETMHLKHLYQVAAVAVLCVQNEPDYRPLITDVAKSLMPLVPVELGGALKNAKALSATEMKPLVAANLSTKTTEKTELKAQI

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
1248			66	33	0.997452
1318	33	66			1.0
1443			62	37	1.0
1448	12		87		0.992554
1466			87	12	0.992554
1470	14	85			0.993533
1474	14			85	0.993533
1478		85		14	0.993533
1479	85		14		0.993533
1480	85			14	0.993533
1538	20		80		0.995493
1541	80	20			0.995493
1543	80	20			0.995493
1547	80			20	0.995493
1579	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain