SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene397

Length: 1994 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene397
ATCGGATACGCAGACAAACAACTGGAGAAACCAGCTGGATTTAGGTTTCAGCTCCTGCTTCCCCCAGTGGTGGTGGTTTCGGTTCCCTGCATCTGGTTTTACGTCGTGACCTTGGCTGTCACTCAAGTTGGGATTCAAATTTTGCATGGCACGATCTTGTTCTAGGTCTATTTTTCGATTCCTTCTCTGCCCTCGTTTTTGTGACTGCTGTTGTAATTTCATTGAAAATCGCTTTCCCTGAATTGAGGCTTGGGTTTAGGCTGCCTTCCATGAGGTTGGTCAGGGGTTCGACCCTGGGCCAGAGTTGGGGTTGCTTGGCTGTTTCATGGGTGAAGCCATTGGGTGTCTTTGCATTCTTGGGTGATGAGAATCACAGGGGAAGAATTAAAGGAAATTCCTCTGTGACATGGACCATTGGCTCCAAGCCTAAGTGTGAAAAAAGCAACAAGAATTTGAAGGAAGGGGTTGAGGTGTACAGCAATGGGGATGTGTATGAGGGGGAGTTCTATCAGGGGAAATGCTCAGGAAGTGGTGTTTATTATTATTATGTCAATGGAAGATATGAGGGTGATTGGGTTAATGGCAAGTATGATGGCCATGGTGTTGAGACTTGGGCTCGTGGTAGCCGTTACCGGGGCCAATACAGGGAGGGCCTGAGGCATGGATATGGTGTTTATATATTTTACACAGGGGATATGTATGCAGGGGAATGGCATAATGGGCAGAGCCATGGGCATGGTGTGCAGACTTGTGAAGATGGAAGTCGGTATGTTGGAGAGTTTAAATGTGGGGTTAAGCATGGACTTGGGTTCTTGCCCTTGGGTGTTGGTGATACATATGCAGGGGAGTATTTTGCAGACAAAATGCATGGCTTTGGAGTTTATAACTTTGCCAATGGGCATAGATATGAAGGAGCATGGCATGATGGGAAAAAGCAAGGTCTTGGAATGTATACATTTAGGAATAGTGAAACACAATCTGGACACTGGCATTGTGGTGTGCTACAGACTCCCAGTACTCATAATTTTCAATCAGGATTAACCAAGGGGGTGAGCCATGCGAAAGTCCTGCATGCAGTGCAGGAGGCAAGACATGCAGCAGCAAAAGCATTTGAAGTGCCAAGAGTTGATGAAAGGGTCAACAAAGCTGTTGCAGCTGCCACCAAAGCAGCCACTGCAGCAAGAGTTGCAGCAGTGAAAGCTGTTCAAAAGAGACTGCATAAAGATGTCCCTCTCGTGGATAGTCTGAATACTTGACTTCTATGAACAACAAATGGCAATTGAATTTGCTAACATACTAAATTTCTGCTAGGAGATAGGAGTTCGGGTTGTTAACTTTGATGCAGCATACTTCCCCATAAACAAGAAAAAATGTCTGCTTATTACAGGCATTTTCATAAAATGGGATAAAACCAGTTTTCATCTCTGGTAATTCAAGTGGTAGAGTGATCTATTCAGAAGTTTGTTTTTCGTTGATATATCTTCATCAGAGAAGTGACTTAAAGTGAATACTAGCGGCCTGTTTTGAGTTCTGTGGATGAAACATGCACCCCTGTGGAATATTGTAGAAAATCCAAGTTTTCTATCAGGTGGTGTGTTCACACAGTGCTCTTGTACAGTGAAATGGAGTTTTAATTAGTGGCTAAGCAAAAGCTTGCCACCTGCCAACCAATATAGCCTTTGTTCAATGTAGAGGCTATTTCAAATATGTATACATAAGCAAGTAATCACGAGATGGTTTTAATATCGAAAATGGCATATCTGTTGCATAAGTTCAAAAGCATCTCGATATGTTTGTGATTTGCTTATTGGTGATTATGTATTTACACACTGTAAAGCTACTGCTGGCGAAGATAAGGTGTGTGTTTCTTGTTAATTAGTGAATTAGTATGTTAAATCTAACATATGAACTAAGGCATTTGACTGATATATGTCAAGATGCTATGTATATTAACTTAATGTAGCTAATACAGCATTAAGTGTATATCAACTAGG

Ace file of the UniGene sp_v2.0_unigene397 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	MORN domain containing protein	-	-	3.0e-05	60%
FL-Next	sp=Phosphatidylinositol-4-phosphate 5-kinase 5; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	33%
Blast2go	protein	-	-	0.0	76%

Source	GOs	Term	Type	Identity
Blast2go	sugar binding	GO:0005529	Molecular Function	76%
Blast2go	peptidase activity	GO:0008233	Molecular Function	76%
Blast2go	plastid	GO:0009536	Cellular Component	76%

Source	InterPros	Term	Type	Identity
Sma3	Insect cuticle protein	IPR000618	-	-
Sma3	MORN motif	IPR003409	-	-
Sma3	Peptidase C14, caspase catalytic	IPR011600	-	-
Sma3	Tubulin, conserved site	IPR017975	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q9SLG9

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 152 aas, your sequence is shorter than subject: 329 - 772

Fln protein: MRLVRGSTLGQSWGCLAVSWVKPLGVFAFLGDENHRGRIKGNSSVTWTIGSKPKCEKSNKNLKEGVEVYSNGDVYEGEFYQGKCSGSGVYYYYVNGRYEGDWVNGKYDGHGVETWARGSRYRGQYREGLRHGYGVYIFYTGDMYAGEWHNGQSHGHGVQTCEDGSRYVGEFKCGVKHGLGFLPLGVGDTYAGEYFADKMHGFGVYNFANGHRYEGAWHDGKKQGLGMYTFRNSETQSGHWHCGVLQTPSTHNFQSGLTKGVSHAKVLHAVQEARHAAAKAFEVPRVDERVNKAVAAATKAATAARVAAVKAVQKRLHKDVPLVDSLNT*

Protein Length: 330

Fln nts: ATCGGATACGCAGACAAACAACTGGAGAAACCAGCTGGATTTAGGTTTCAGCTCCTGCTTCCCCCAGTGGTGGTGGTTTCGGTTCCCTGCATCTGGTTTTACGTCGTGACCTTGGCTGTCACTCAAGTTGGGATTCAAATTTTGCATGGCACGATCTTGTTCTAGGTCTATTTTTCGATTCCTTCTCTGCCCTCGTTTTTGTGACTGCTGTTGTAATTTCATTGAAAATCGCTTTCCCTGAATTGAGGCTTGGGTTTAGGCTGCCTTCC_-_ATGAGGTTGGTCAGGGGTTCGACCCTGGGCCAGAGTTGGGGTTGCTTGGCTGTTTCATGGGTGAAGCCATTGGGTGTCTTTGCATTCTTGGGTGATGAGAATCACAGGGGAAGAATTAAAGGAAATTCCTCTGTGACATGGACCATTGGCTCCAAGCCTAAGTGTGAAAAAAGCAACAAGAATTTGAAGGAAGGGGTTGAGGTGTACAGCAATGGGGATGTGTATGAGGGGGAGTTCTATCAGGGGAAATGCTCAGGAAGTGGTGTTTATTATTATTATGTCAATGGAAGATATGAGGGTGATTGGGTTAATGGCAAGTATGATGGCCATGGTGTTGAGACTTGGGCTCGTGGTAGCCGTTACCGGGGCCAATACAGGGAGGGCCTGAGGCATGGATATGGTGTTTATATATTTTACACAGGGGATATGTATGCAGGGGAATGGCATAATGGGCAGAGCCATGGGCATGGTGTGCAGACTTGTGAAGATGGAAGTCGGTATGTTGGAGAGTTTAAATGTGGGGTTAAGCATGGACTTGGGTTCTTGCCCTTGGGTGTTGGTGATACATATGCAGGGGAGTATTTTGCAGACAAAATGCATGGCTTTGGAGTTTATAACTTTGCCAATGGGCATAGATATGAAGGAGCATGGCATGATGGGAAAAAGCAAGGTCTTGGAATGTATACATTTAGGAATAGTGAAACACAATCTGGACACTGGCATTGTGGTGTGCTACAGACTCCCAGTACTCATAATTTTCAATCAGGATTAACCAAGGGGGTGAGCCATGCGAAAGTCCTGCATGCAGTGCAGGAGGCAAGACATGCAGCAGCAAAAGCATTTGAAGTGCCAAGAGTTGATGAAAGGGTCAACAAAGCTGTTGCAGCTGCCACCAAAGCAGCCACTGCAGCAAGAGTTGCAGCAGTGAAAGCTGTTCAAAAGAGACTGCATAAAGATGTCCCTCTCGTGGATAGTCTGAATACTTGACTTCTATGAACAACAAATGGCAATTGAATTTGCTAACATACTAAATTTCTGCTAGGAGATAGGAGTTCGGGTTGTTAACTTTGATGCAGCATACTTCCCCATAAACAAGAAAAAATGTCTGCTTATTACAGGCATTTTCATAAAATGGGATAAAACCAGTTTTCATCTCTGGTAATTCAAGTGGTAGAGTGATCTATTCAGAAGTTTGTTTTTCGTTGATATATCTTCATCAGAGAAGTGACTTAAAGTGAATACTAGCGGCCTGTTTTGAGTTCTGTGGATGAAACATGCACCCCTGTGGAATATTGTAGAAAATCCAAGTTTTCTATCAGGTGGTGTGTTCACACAGTGCTCTTGTACAGTGAAATGGAGTTTTAATTAGTGGCTAAGCAAAAGCTTGCCACCTGCCAACCAATATAGCCTTTGTTCAATGTAGAGGCTATTTCAAATATGTATACATAAGCAAGTAATCACGAGATGGTTTTAATATCGAAAATGGCATATCTGTTGCATAAGTTCAAAAGCATCTCGATATGTTTGTGATTTGCTTATTGGTGATTATGTATTTACACACTGTAAAGCTACTGCTGGCGAAGATAAGGTGTGTGTTTCTTGTTAATTAGTGAATTAGTATGTTAAATCTAACATATGAACTAAGGCATTTGACTGATATATGTCAAGATGCTATGTATATTAACTTAATGTAGCTAATACAGCATTAAGTGTATATCAACTAGG

Fln Alignment:

Contig397___GNSSVTWTIGSKPKCEKSN-KNLKEGVEVYSNGDVYEGEFYQGKCXXXXXXXXXVNGRYEGDWVNGKYDGHGVETWARGSRYRGQYREGLRHGYGVYIFYTGDMYAGEWHNGQSHGHGVQTCEDGSRYVGEFKCGVKHGLGFLPLGVGDTYAGEYFADKMHGFGVYNFANGHRYEGAW
Q9SLG9_____________GHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGATYEGEFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQGKYQWSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGHWSKDPEEMNGTY-YPSGNEGNLEW

SNPs (tot: 113)

Position	A %	C %	G %	T %	Probability
418	90			10	0.90298
525	87	12			0.992554
526		12		87	0.992554
539		85		14	0.993533
540	85		14		0.993533
542	85		14		0.993533
543		85		14	0.993533
544			14	85	0.993533
545	14			85	0.993533
547	12			87	0.992554
549	87	12			0.992554
550	87	12			0.992554
551	12			87	0.992554
554	87		12		0.992554
559	12			87	0.992554
561			12	87	0.992554
577	57		42		1.0
593			14	85	0.993533
594	14			85	0.993533
595		14		85	0.993533
597			14	85	0.993533
600	14	85			0.993533
602			14	85	0.993533
603			14	85	0.993533
605		14		42	1.0
607		14		85	0.993533
610	85	14			0.993533
611	85		14		0.993533
642		16		83	0.994512
712	14			85	0.993533
713		14		85	0.993533
719	85		14		0.993533
720			14	85	0.993533
724	85	14			0.993533
725			14	85	0.993533
726	14			85	0.993533
741	83	16			0.994512
742		16		83	0.994512
744			83	16	0.994512
773		10	90		0.90298
774		10	90		0.90298
775		10	90		0.90298
820		11		88	0.923564
822		11		88	0.923564
886	83	16			0.994512
897	16	83			0.994512
899			16	83	0.994512
900	16			83	0.994512
904	14			85	0.993533
907		14		85	0.993533
908	14		85		0.993533
909	14	85			0.993533
910	14		85		0.993533
915	14			85	0.993533
916		14		85	0.993533
918	14	85			0.993533
920			14	85	0.993533
1014	16		83		0.994512
1017	83			16	0.994512
1019	16			83	0.994512
1022		83	16		0.994512
1026	83	16			0.994512
1027		16		83	0.994512
1028	16			83	0.994512
1029	16		83		0.994512
1030			16	83	0.994512
1033		16		83	0.994512
1034	16			83	0.994512
1038			16	83	0.994512
1173		50	50		1.0
1174	50			50	1.0
1175		50	50		1.0
1176		50		50	1.0
1177	50		50		1.0
1178	75			25	0.996473
1179		50		50	1.0
1180	50			50	1.0
1181		25	50	25	0.995533
1182	25	50		25	0.995533
1183		75	25		0.996473
1184	75			25	0.996473
1187			66	33	0.997452
1188			33	66	0.997452
1189		66	33		0.997452
1190	33		33	33	0.997753
1191	33	66			1.0
1192			66	33	0.997452
1193	66			33	0.997452
1194	33	66			0.997452
1195	33	33	33		0.997753
1814		66		33	1.0
1820	33		66		1.0
1824	66	33			1.0
1825		33	66		1.0
1830	66			33	1.0
1831	66	33			1.0
1832	33			66	1.0
1836	66	33			1.0
1837	66		33		1.0
1865	25		75		0.996473
1887	75	25			0.996473
1889			25	75	0.996473
1890			75	25	0.996473
1891	75			25	0.996473
1892		75		25	0.996473
1893	75			25	0.996473
1896	25	75			0.996473
1897	75	25			0.996473
1898	75			25	0.996473
1901		25		75	0.996473
1903	75			25	0.996473
1907		75		25	0.996473
1908	25		75		0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
1447	1459	ATA ATA ATA ATA A	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain