SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene361

Length: 2081 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

>sp_v2.0_unigene361
CTTTAATAATGGGTGCTTCCGACTGAAATTTTGTTAGCTGGCATTTTCCAAATGACGGAGTGCGTTCTGTAGCCGAGATTTCCTTGGCCTCCGCAATTTCTGTGCAAAAGTTTAACAGGCAATAGACGCGAAGGAACGGGGAAAGAATTGCTTCTTGAAGTATGGAGATGATTGAGAATTTTTTGGTGAAGATCGAAGAGGGAAAGGAAGAGCAGAACGGCAACCCTTCAGTCGGCCCTGCTTACCGAAACATATTAGCAAAGGATGGATTCGCGCCTCTGCCGGAAGGGATCGAGTGCTGCTGGGACCTTCTCAGCCAATCTGCCCAAAAGTATCCGCATAATCGAATGCTTGGTCGACGTGATATTGAAAATGGAAAGGCAGGAAAATATGTATGGTTGACATATCAGGAAGCATATGAAAGATCAATCGAAATTGGATCAGCTATAAGGAGCTCTGATGTTGGTCCAGGAGGCCGTTGTGGTATTTATGGCATTAACTCTTCCGAATGGATTATCAGCATGGAGGCTTGCAACAGCCATGGGATTTATTGTGTTCCGCTCTATGACACTCTTGGTGCGGACGCTATTGAGTTCATTATTTGCCATGCTGAAGTTTCACTGGTTTTTGTACAAGAGAGCAAGATACCTGCAGTGCTGAATTGTTTGCCGAGGTGTACAGGATATTTAAAGACTTTAGTAAGCTTTGGTAACATAACAGAAATCCATAAAGAAGCAGCTGATAGTCTTGGAGTGAAAGCATACTCTTGGCATGACTTCTTATTTCTGGGAAAGAAGACAAGATGTGTCCCTTCACCACCCAAGAAGAGTGATATTTGCACAATTATGTATACGAGTGGAACAACAGGAGAACCTAAAGGTGTTATGTTAACGAATGAAAACATCATTGCAGAAATTGCCGCAATTGATCATTTGCTTTCTAATACATTTACAGAGGTGATAGAAACAGACATTTACTTTTCTTTTCTCCCCTTAGCACATGTATTTGATCGAGTTATTGAGGAATATTGCATTTTTAAAAGTGCATCAATAGGCTTCTGGCGTGGGGATGTGAAGCTTTTGGTGGAAGATATTATGGAGTTAAAGCCAACTATTTTATGTGGTGTCCCTCGTGTGTTCGATCGTATATACACAGGTATAAATGAGAAGATATCAATTGGCGGAGCATTAAAGAAAATGCTGTTTGATTTTGCATACAGCTACAAGTTAAAGAACATGCAGAAGGGTTTGAAGCAGGATGAAGCAGCACCACTTTTTGATAAGCTTGTTTTTACTAAGGTAAAACAAGGTCTAGGAGGTTGTCTTCGTCTGATCCTTTCTGGAGCAGCCCCACTGGCTAGACACGTAGAAGAGTTTTTGAGAGTTACGAGCTGTAGTGTTGTAGTACAAGGATATGGTTTGACTGAAACATGTGCTGGAAGTTTTAGTTCCTTGGCCAATGTCTTCTCCATGGTTGGAACAGTTGGAGTGCCTGTTCCATGCATTGAAGTGCGGCTTAAGTCGGTCCCTGAGATGGGCTATGATGCACTTTCCAGAACACCACGTGGAGAGATTTGTATAAGGGGAAACACCATATTTTGTGGGTATCACAAGCGTGAAGATCTGACTAGTGATGTTCTTATGGATGGTTGGTTTCACACAGGTGATATTGGCGAGTGGCTACCAAATGGATCATTGAAAATCATTGATAGGAAAAAAAATATTTTCAAACTTTCACAGGGTGAATACGTTGCAGTGGAAAATTTAGAGAATGTATACAGCCGTTGTTCTGCTATTGCTTCGGTATGCAGGTCTAACATATGTTGTTTTCTTAGAAACTTCAAACAGACTTAGCATCTAAGAAGCGAAACTATTGACCTTGTTGGAAGTTAGATAGTGCTATATATCTTCTGCTGCTAGAACCCATTTACGTTTGTGCTTCTTGAAAGCAATGGTTAATGATCATAATTCTCTCCGAGTCTAGTTACAAATGGTTTCTGAATCTATGAAATGTGCATACATAAACCCTGTACAGCAAGTCTGCCAGCATGACTTTAGAAACACTTTTATGATTAGCAACTT

Ace file of the UniGene sp_v2.0_unigene361

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Long-chain acyl-CoA synthetase 4 n=2 Tax=Ricinus communis RepID=B3TZ27_RICCO	-	-	63%
FL-Next	tr=4-coumarate:CoA ligase; Pinus massoniana.	-	-	27%
Blast2go	annotation was added to scaffolds in november 2011~long-chain-fatty-acid- ligase	-	-	77%

Source	GOs	Term	Type	e value	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	0.0	77%
Blast2go	ligase activity	GO:0016874	Molecular Function	0.0	77%

Source	InterPros	Term	Type	e value	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: C1KBY2

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 57 aas, putative chimeric sequence,

Fln protein: MEMIENFLVKIEEGKEEQNGNPSVGPAYRNILAKDGFAPLPEGIECCWDLLSQSAQKYPHNRMLGRRDIENGKAGKYVWLTYQEAYERSIEIGSAIRSSDVGPGGRCGIYGINSSEWIISMEACNSHGIYCVPLYDTLGADAIEFIICHAEVSLVFVQESKIPAVLNCLPRCTGYLKTLVSFGNITEIHKEAADSLGVKAYSWHDFLFLGKKTRCVPSPPKKSDICTIMYTSGTTGEPKGVMLTNENIIAEIAAIDHLLSNTFTEVIETDIYFSFLPLAHVFDRVIEEYCIFKSASIGFWRGDVKLLVEDIMELKPTILCGVPRVFDRIYTGINEKISIGGALKKMLFDFAYSYKLKNMQKGLKQDEAAPLFDKLVFTKVKQGLGGCLRLILSGAAPLARHVEEFLRVTSCSVVVQGYGLTETCAGSFSSLANVFSMVGTVGVPVPCIEVRLKSVPEMGYDALSRTPRGEICIRGNTIFCGYHKREDLTSDVLMDGWFHTGDIGEWLPNGSLKIIDRKKNIFKLSQGEYVAVENLENVYSRCSAIASVCRSNICCFLRNFKQT*

Protein Length: 565

Fln nts: CTTTAATAATGGGTGCTTCCGACTGAAATTTTGTTAGCTGGCATTTTCCAAATGACGGAGTGCGTTCTGTAGCCGAGATTTCCTTGGCCTCCGCAATTTCTGTGCAAAAGTTTAACAGGCAATAGACGCGAAGGAACGGGGAAAGAATTGCTTCTTGAAGT_-_ATGGAGATGATTGAGAATTTTTTGGTGAAGATCGAAGAGGGAAAGGAAGAGCAGAACGGCAACCCTTCAGTCGGCCCTGCTTACCGAAACATATTAGCAAAGGATGGATTCGCGCCTCTGCCGGAAGGGATCGAGTGCTGCTGGGACCTTCTCAGCCAATCTGCCCAAAAGTATCCGCATAATCGAATGCTTGGTCGACGTGATATTGAAAATGGAAAGGCAGGAAAATATGTATGGTTGACATATCAGGAAGCATATGAAAGATCAATCGAAATTGGATCAGCTATAAGGAGCTCTGATGTTGGTCCAGGAGGCCGTTGTGGTATTTATGGCATTAACTCTTCCGAATGGATTATCAGCATGGAGGCTTGCAACAGCCATGGGATTTATTGTGTTCCGCTCTATGACACTCTTGGTGCGGACGCTATTGAGTTCATTATTTGCCATGCTGAAGTTTCACTGGTTTTTGTACAAGAGAGCAAGATACCTGCAGTGCTGAATTGTTTGCCGAGGTGTACAGGATATTTAAAGACTTTAGTAAGCTTTGGTAACATAACAGAAATCCATAAAGAAGCAGCTGATAGTCTTGGAGTGAAAGCATACTCTTGGCATGACTTCTTATTTCTGGGAAAGAAGACAAGATGTGTCCCTTCACCACCCAAGAAGAGTGATATTTGCACAATTATGTATACGAGTGGAACAACAGGAGAACCTAAAGGTGTTATGTTAACGAATGAAAACATCATTGCAGAAATTGCCGCAATTGATCATTTGCTTTCTAATACATTTACAGAGGTGATAGAAACAGACATTTACTTTTCTTTTCTCCCCTTAGCACATGTATTTGATCGAGTTATTGAGGAATATTGCATTTTTAAAAGTGCATCAATAGGCTTCTGGCGTGGGGATGTGAAGCTTTTGGTGGAAGATATTATGGAGTTAAAGCCAACTATTTTATGTGGTGTCCCTCGTGTGTTCGATCGTATATACACAGGTATAAATGAGAAGATATCAATTGGCGGAGCATTAAAGAAAATGCTGTTTGATTTTGCATACAGCTACAAGTTAAAGAACATGCAGAAGGGTTTGAAGCAGGATGAAGCAGCACCACTTTTTGATAAGCTTGTTTTTACTAAGGTAAAACAAGGTCTAGGAGGTTGTCTTCGTCTGATCCTTTCTGGAGCAGCCCCACTGGCTAGACACGTAGAAGAGTTTTTGAGAGTTACGAGCTGTAGTGTTGTAGTACAAGGATATGGTTTGACTGAAACATGTGCTGGAAGTTTTAGTTCCTTGGCCAATGTCTTCTCCATGGTTGGAACAGTTGGAGTGCCTGTTCCATGCATTGAAGTGCGGCTTAAGTCGGTCCCTGAGATGGGCTATGATGCACTTTCCAGAACACCACGTGGAGAGATTTGTATAAGGGGAAACACCATATTTTGTGGGTATCACAAGCGTGAAGATCTGACTAGTGATGTTCTTATGGATGGTTGGTTTCACACAGGTGATATTGGCGAGTGGCTACCAAATGGATCATTGAAAATCATTGATAGGAAAAAAAATATTTTCAAACTTTCACAGGGTGAATACGTTGCAGTGGAAAATTTAGAGAATGTATACAGCCGTTGTTCTGCTATTGCTTCGGTATGCAGGTCTAACATATGTTGTTTTCTTAGAAACTTCAAACAGACTTAG___CATCTAAGAAGCGAAACTATTGACCTTGTTGGAAGTTAGATAGTGCTATATATCTTCTGCTGCTAGAACCCATTTACGTTTGTGCTTCTTGAAAGCAATGGTTAATGATCATAATTCTCTCCGAGTCTAGTTACAAATGGTTTCTGAATCTATGAAATGTGCATACATAAACCCTGTACAGCAAGTCTGCCAGCATGACTTTAGAAACACTTTTATGATTAGCAACTT

Fln Alignment:

Contig361___KTRCVPSPPKKSDICTIMYTSGTTGEPKGVMLTXXXXXXXXXXX-DHLLSNTFTEVIETDIYFSFLPLAHVFDRVIEEYCIFKSASIGFWRGDVKLLVEDIMELKPTILCGVPRVFDRIYTGINEKISIGGALKKMLFDFAYSYKLKNMQKGLKQDEAAPLFDKLVFTKVKQGLGG-----CLRLILSGAAPLARHVEEFLRVTSCSVVV-QGYGLTET---CAGSFSSLANVFSMV-GTVGVPVPCIEVRLKSVPEMGYDALSRTPRGEICIRGNTIFCGY-HKREDLTSDVLMDGWFHTGDIGEWLPNGSLKIIDRKKNIFKLSQGEYVAVENLE
C1KBY2_____________ETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFH--SEDVILCVLPLFHIYS------------------------------LNSVLLCALR----------------AGAATLIMQKFNLTTCLELIQK--YKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDT-ETG-ESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKY-KGFQVAPAELE

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
629			20	80	0.995493
630			80	20	0.995493
635	40			60	1.0
1421	10		90		0.90298
1784		11		88	0.923564
1803	33		66		1.0
1808		85	14		0.993533
1809	85		14		0.993533
1810			85	14	0.993533
1811			85	14	0.993533
1813	14	85			0.993533
1815	85		14		0.993533
1816	85		14		0.993533
1821			14	85	0.993533
1822		14	85		0.993533
1825		16	83		0.994512
1826			16	83	0.994512
1827	16			83	0.994512
1828	16			83	0.994512
1833	83			16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain