SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene298

Length: 1941 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene298 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	rhomboid-like protein 15 [Arabidopsis thaliana] sp\|Q8LB17.2\|Y3846_ARATH RecName: Full=Uncharacterized protein At3g58460 gb\|AAM97052.1\| putative protein [Arabidopsis thaliana] gb\|AAN15505.1\| putative protein [Arabidopsis thaliana] gb\|AEE79784.1\| rhomboid-l	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Blast2go	rhomboid family protein	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on peptide bonds (peptide hydrolases), Serine endopeptidases.	EC:3.4.21.-	-	0.0	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	serine-type endopeptidase activity	GO:0004252	Molecular Function	0.0	72%
Blast2go	integral to membrane	GO:0016021	Cellular Component	0.0	72%

Source	InterPros	Term	Type	Identity
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal	IPR000449	-	-
Sma3	Peptidase S54, rhomboid	IPR002610	-	-
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote	IPR015940	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8E7

Fln msg:

Fln protein: MRSNIVSEASWSTRVGQWWVGVPFITSGLVAVCGIIYLICLLVGYDSFYEICFWPAQLVLRLQVYRIYTSVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYAWILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLTSYTAIENSAMLACCVRRPGFIVGGASGAAVNGSLPSFPSTASSGAGLSNFWRNVRSWMPPRESLPNQSLQDPRFPGRGRTLVSSQIQSTSRIGSETELQTRLLDRSSQPQAPSPMMPIITRETVTSNARHTHRENSQQSGPTHMNQGHTQTHDEAIQNLVAMGFEKAKAEVALAAANGDLALAVELLSSQE*

Protein Length: 413

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTCTGGGGGAAGCGGGCCAAGGAAGAACGCTTATGTAACAAATTCTGTGGAACACCCAACTGAACGAAATTGATAGAAAAACCACATCATGTTGATGTGCATAATATTTTAAATATTTTAGTACGACAGGAATTCTGCAAGTTTTGGATTGGCAGC_-_ATGAGATCCAACATTGTTTCAGAGGCCAGTTGGTCCACCAGAGTTGGACAGTGGTGGGTGGGAGTTCCATTTATCACATCTGGATTGGTGGCTGTTTGTGGTATTATTTACTTGATATGCCTGCTGGTTGGATATGATTCCTTTTATGAGATCTGTTTCTGGCCAGCACAGTTAGTTTTGCGATTGCAAGTGTATAGGATCTACACCTCTGTGGTATTTCATGGTTCGGTGCTGCATGTTATGTTCAATATGATGGCCTTGGTGCCTATTGGCAGTGGGCTAGAGCGAATTATGGGATCGGTTCGATACTTGCATGTCACATTGCTGCTAGCTACCTGCAATGCGGTTTTGCATCTTCTCATTGCTTATCTGGCAGCGCACAACCCAGTGCATCCTTATCCTTTCTTTATGGATGAGTGTACCATTGGCTTCTCTGGTATCATATTTGCTATGATTGTCATGGAAACTAGCTTAAATGGAAATCAGAGTCGAAGTGTATTTGGACTTTTCAATGTCCCTGGAAAATGGTATGCTTGGATCCTTCTTATCCTGTTCCAGCTTCTAATGCCAAATGTTTCACTCTTAGGACATCTCTGTGGCATTTTGTCTGGGTTTGCATATACCTATGGCTTGTTTAACTACTTCTTATTGGGATTAACATCATATACAGCTATCGAGAACTCTGCAATGCTTGCATGCTGTGTAAGGCGCCCTGGATTCATCGTTGGTGGTGCAAGTGGTGCTGCAGTTAATGGATCACTTCCTTCTTTTCCAAGTACAGCGTCTAGTGGTGCAGGTTTAAGTAATTTTTGGAGAAATGTCCGTTCATGGATGCCACCAAGGGAAAGTTTGCCTAATCAGTCTTTGCAAGATCCTAGATTTCCAGGGAGAGGTCGGACGCTTGTGTCTTCCCAAATTCAATCAACATCAAGGATTGGTTCTGAGACAGAACTACAAACTAGACTTCTTGATAGGAGCAGCCAACCACAAGCACCTTCTCCAATGATGCCAATAATCACTCGTGAGACTGTAACATCAAATGCAAGGCACACACATAGGGAAAATTCACAACAATCAGGCCCTACACATATGAATCAGGGACATACACAAACTCATGATGAAGCCATTCAGAATCTTGTTGCAATGGGATTTGAAAAGGCTAAGGCAGAAGTTGCACTAGCTGCAGCAAATGGAGATTTAGCTCTTGCTGTTGAACTTCTGAGCAGTCAGGAATAA___CAGCATTCTAAATTTTTCTTTAAATTAGCATGGAAGTTCGCAACATGTTTAAGATGAGGATAAAGTTCTCCAATCCTGGAGCGGAAATGTGCATTACACATGGTTCCAATGTAAATAAATGCAGTATTTTTTGTCTTGCTTCTTCCAATTTCCCAGTTTGCTTGCCAATGGATTTTAATTGAGTGCTTTCTCCATGTCAAGAGCAAGTCTTAATGAGAATTAAAATGTTCCAATACTGGATGAAGCTCATTCAGGGCCTTCTGTTATAGTTGGCTTGTTTCTTTGTTTTCATCAGACCATACTATTTAAGAGGTTCTACCCCAAAGAACTAGGGAAAAAAATCAGAGTGGCTGAATTTAATTTGAATTCAATCCAAATTATACTACAACAAACAACAGAATTGATAGTGTATGCCATTCTGAATTATGCACTGTACTTAGATGAGAGGAATTTAACTTTTTATCATAATAGATAACCTTGCCAAGATTTGTTAATGCTTAACTGTTGCTTTATTTGAGTCTAC

Fln Alignment:

Contig298___MRSNIVSEASWSTRVGQWWVGVPFITSGLVAVCGIIYLICLLVGYDSFYEICFWPAQLVLRLQVYRIYTSVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYAWILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLTSYTAIENSAMLACCVRRPGFIXXXXXXXXXXXSLPSFPSTASSGAGLSNFWRNVRSWMPPRESLPNQSLQDPRFPGRGRTLVSSQIQSTSRIGSETELQTRLLDRXXXXXXXXXXXPIITRETVTSNARHTHRENSQQSGPTHMNQGHTQTHDEAIQNLVAMGFXXXXXXXXXXXXXXXXXXAVELLSSQE
D5A8E7_____________MRSNIVSEASWSTRVGQWWAGVPFITSGLVAVCGIIYLVCLLVGYDSFYAICFWPAQLILRLQVYRIYTSVVFHGSVLHVMFNMMALVPIGSGLERIMGSVRYLHVTLLLATCNAVLHLLIAYLAAHNPVHPYPFFMDECTIGFSGIIFAMIVMETSLNGNQSRSVFGLFNVPGKWYASILLILFQLLMPNVSLLGHLCGILSGFAYTYGLFNYFLLGLASYTAIESSAMLACCVRRPGFIIGGASGAAATGSLPSFPSTASSGAGLGNVWRSARSLMPQRETLPNQSLQDPRFPGRGRTLGSSQIQSTSRVGSETELQTRLLERSSQPEAPSQMMPISTRETATSNARHTPRENSQESGPTHINQEHTQNH-ETIQNLVAMGFEKAKAEVALDAANGDVALAVELLSSQE

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
1250		33		66	0.997452
1336	91			8	0.999807
1488			85	14	0.999981
1513			10	90	0.90298
1518		90	10		0.90298
1519	90	10			0.90298
1520		10		90	0.90298
1523		90	10		0.90298
1524	90		10		0.90298
1529		10	90		0.90298
1530		88	11		0.923564
1533		88	11		0.923564
1541	12		87		0.992554
1542	87	12			0.992554
1544	85			14	0.993533
1548		83		16	0.994512
1550		83	16		0.994512
1551	83			16	0.994512
1554		20	80		0.995493
1556		20		80	0.995493
1557		20	80		0.995493
1558	20			80	0.995493
1561	80		20		0.995493
1565	20	20		60	0.99255
1566	60	40			1.0
1567		20	20	60	0.99255
1569		80		20	0.995493
1570	60		40		1.0
1857	80	20			0.995493
1858		20	80		0.995493
1859	20			80	0.995493
1894		14		85	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain