SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene292

Length: 1589 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene292

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[R] COG0661 Predicted unusual protein kinase	-	-	1.99965e-42	29%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	82%
Blast2go	probable serine threonine-protein kinase abkc-like	-	-	0.0	86%

Source	GOs	Term	Type	Identity
Blast2go	phosphorylation	GO:0016310	Biological Process	86%
Blast2go	kinase activity	GO:0016301	Molecular Function	86%
Blast2go	mitochondrion	GO:0005739	Cellular Component	86%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	ABC-1	IPR004147	-	-
Sma3	DNA/RNA non-specific endonuclease, active site	IPR018524	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LL04

Fln msg: Separated hits, possible frame ERROR between 111 and 129, your sequence is shorter than subject: 394 - 657

Fln protein: RRDLGCHSDLCNELTKLQTKAPAHKFSYTRQTIEKAxxxxxxxDTQFEDFEEEPVASGSIAQVHKATLRFRYAGQKMNPMTVAVKVRHPGVSEVIRRDFVIINWVAKVSSRLPALRWLRLDESVQQFAVFMLSQVDLAREAAQLSRFLYNFRRWKDVSFPKPLYPLVHPEVLVETYEQGESVARYVDQPEGHNRISKSLAHIGTHTLLKMLLVDNFIHADMHPGNILVRMGSGNPSSKRLFRSRPHLVFLDVGMTAELSNNDRLNLLELFKSIALRDGRTAAECTLKFSRNQSCPDPNAFIQEVEASFKFWGSPEGDVVHPAECMNELLEQVRRHKVNIDGNVCTVMVTTLVLEGWQRKLDPDYNVMGTLQTLLFKADWAKSLSYTIERLMAP*

Protein Length: 395

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGATTTATGTAATGAGCTTACAAAATTGCAGACAAAGGCACCGGCACATAAATTTTCATATACACGGCAAACTATTGAAAAGGCTTTxxxxxxxxxxxxxxxxxxTGACACTCAATTTGAGGATTTTGAAGAGGAGCCAGTGGCTTCTGGCAGTATTGCACAAGTCCATAAAGCTACTCTGAGATTTCGTTATGCCGGTCAGAAGATGAACCCTATGACTGTTGCAGTGAAAGTCAGGCATCCTGGAGTTAGTGAAGTGATTCGGAGGGATTTTGTGATAATAAATTGGGTTGCAAAGGTTTCTAGTCGTCTGCCGGCACTGAGATGGCTGCGACTTGATGAAAGTGTACAACAATTTGCTGTCTTTATGTTGTCTCAAGTTGATCTTGCCAGAGAAGCTGCACAGTTGAGTCGATTTCTTTACAATTTCCGCAGGTGGAAAGACGTGTCTTTTCCCAAGCCCTTATATCCACTTGTACACCCAGAAGTTTTGGTAGAGACTTATGAACAAGGCGAGAGTGTGGCACGCTATGTTGATCAACCGGAAGGCCACAATCGTATCAGTAAATCACTTGCTCACATTGGCACACATACTCTCCTCAAGATGCTACTGGTGGATAATTTCATTCATGCAGATATGCATCCTGGAAATATTCTTGTGCGAATGGGATCAGGTAATCCTTCAAGCAAGCGACTTTTTCGATCAAGGCCTCATTTGGTGTTCCTTGATGTAGGAATGACAGCAGAACTTTCCAATAATGATCGGCTGAATCTTCTGGAGTTATTCAAATCTATTGCACTAAGGGATGGCCGGACAGCTGCAGAATGTACTCTGAAGTTTTCAAGAAACCAGAGCTGTCCAGATCCAAATGCCTTCATACAGGAAGTTGAGGCTTCATTCAAATTTTGGGGTTCACCTGAAGGTGATGTGGTACATCCAGCAGAGTGCATGAACGAATTGCTTGAACAAGTGAGACGCCACAAAGTGAATATTGATGGGAATGTATGTACTGTAATGGTCACAACATTGGTTCTCGAGGGGTGGCAGCGCAAGTTGGATCCAGATTATAATGTCATGGGCACCTTGCAGACTCTTCTTTTTAAAGCTGATTGGGCAAAATCTCTTTCTTATACAATCGAACGACTCATGGCCCCATAATTTTCATTAGAGCTATTTGGTCGTGCTTTGTCAGAACACTTTGACATTTTCTTGACCCTCAGTACTTTTATCTAAAAGTAATGTTGGAGGATCTAAAAGTTACAAAATGAATGGAGACTTCTGTCAGACTCGAGAGGCATGTGCCGTTCAAAATTCCAACCTTGATATGATTTTCATATTTATTCACTGTTTTCCCCAATTTTGGATATTTATCATTTCTTATTAGAGTTGTCAAATAATTGCGGTAGCGTCCCTTTAACTGAGTGGAAGCCCGATAGAAAACAGGAAGAGAAAGTGATTAAGGTATTGTTGTAATGTCTGAGAGCATCAACCAATGTTCGGATTATATTACTGTACATGATTTTAAAGTCTCCATTGTTTCACTGTACCATGGAATATCAATATTCA

Fln Alignment:

Contig292___DLCNELTKLQTKAPAHKFSYTRQTIEKAFxxxxxxDTQFEDFEEEPVASGSIAQVHKATLRFRYAGQKMNPMTVAVKVRHPGVSEVIRRDFVIINWVAKVSSRLPALRWLRLDESVQQFAVFMLSQVDLAREAAQLSRFLYNFRRWKDVSFPKPLYPLVHPEVLVETYEQGESVARYVDQPEGHNRISKSLAHIGTHTLLKMLLVDNFIHADMHPGNILVRMGSGNPSSKRLFRSRPHLVFLDVGMTAELSNNDRLNLLELFKSIALRDGRTAAECTLKFSRNQSCPDPNAFIQEVEASFKFWGSPEGDVVHPAECMNELLEQVRRHKVNIDGNVCTVMVTTLVLEGWQRKLDPDYNVMGTLQTLLFKADWAKSLSYTIERLMAP
B8LL04_____________DLCKELAKLHTKAPAHKFSYTQHTIEKAFxxxxxxDEIFEDFEEEPVASGSIAQVHKATLKFRYPGQKVKPMIVAVKVRHPGVSEVIRRDFVIINWVAKLSSCLPALRWLRLEESVQQFAVFMLSQVDLSREAAHLNRFLYNFRRWKDVSFPKPLYPLVHPAVLVETYEQGESVARYVDQPEGNYRFNRSLAHTGTHTLLKMLLVDNFIHADMHPGNILVRMGQSNASSKRLFQSRPHVVFLDVGMTAELSKNDRLNILEFFKAVAQRDGRTAAECTLKFSRYQSCPDPNAFIQEVESSFKYWGSPEGDVVHPAECMQELLEHVRHHKVNIDGNVCTVMVTTLVLEGWQRKLDPDFNVMNTLHTVLIRSDWAKSLSYTIEELMAP

SNPs (tot: 3)

Position	A %	C %	G %	T %	Probability
672	88	11			0.923564
675	88			11	0.923564
1000	15		84		0.999986

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain