SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene215

Length: 3177 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene215
CGTCGTGACCTTGGCTGTCAGTGGATATGTATCGTACCTACAGCAGGAGCTGAGAAATAATTGGTTACGCATCCCTTGCCTCATGAATTAATCCATGCTGTGTGTCACATGCTGCGTACATTATTTAGAGGGTTTCTGTGATCTGATCGTTGAACGATTTGAAGGTTTGTGTTGGGCTTTTGAGCTTATTCAGCATCACCAGGGTTACATTGAAAAATGGTAGTGCAAACTTCCAGTATGATTGGTCAGGTTCAACAAGCTCATCGATTTGATGAGAGCTCTCTTATTCGTTACGCTCAGGCAAATGTTGAAGGCTTCCCACTTCCTGTGACAAAATTCAAAGTTTCACAGTTTGGCCATGGACAATCAAATCCAACATTTTTTCTCGAAGTTGAGTCATATACAACCGTCAAACAATATGTTCTGCGAAAAAAGCCTCCTGGAAAGTTGCTCCAGTCTGCACATGCAATTGAAAGGGAATTTGAGGTGTTGGCAGCTTTAGGCAAATATTCAAATGTTCCAGTCCCAAAGGTTTTTTGTTTTTGTACTGATACAAATGTAATTGGTACAACCTTTTACATCATGGAGTATTTGGAAGGTCGGATTTTTCTGAATCCTGGACTGCCGGAACTCAACCCAAAACAAAGAAGAGCCATATACCTTGCGACTACCAAAACTTTGGCAGCAATACACAATGTGGATGTTGATGCGGTAGGATTAGGTCACTATGGACGCTGTGAAAATTACTGCAAGAGGCAGGTAGAAAGATGGGCAAAGCAATATCTAGCTTCAACTGGTGAAGGAAAACCAGATCGAAGTCCAAGGATGTTGGAACTCATTGATTGGCTCAAGCAACACATACCAGCTGACGTCGTGACCTTGAGCTCAAAGACTGGTCTTGTTCATGGAGACTTTCGGATTGATAATCTTCTGTTCCATCCATCAGAGGAACGTGTCATTGGTGTTTTAGATTGGGAATTATCAACATTGGGGGACCCAATGTCTGATGTTGCTTACAACTGCATGTGTTTTCTCATCAACAACAATGAGTTTCCAGTTCCTCATTATGGCTTTGATAATGGGATAATGCCAGAAGGAGTTCCTTCTCTGGAAGAGCATCTGGCGAATTATTGTGCTGCAGCTGGTACCCAGTGGCCTGCTAAAGATTGGAAGTTTTATATTGCATTCTCTCTTTTTCGTTCAGCATCTATTTTTGCGGGGGTTTATTATAGGTGGTTGAAGGGAAATGCCTCAGGGGGGGAACGTGCCAAGTCTGCTGGACGCTTAGCTATATATTTTATTGATACAGCTTGGAAGTTTATAAGTAGGGATTCAGTACTTCCAGACAGGCCACCATCTTCTGCTTTTGGAAATGTACCATTGGAAACAGATGTCTCTTTATCCCATATTCCAGCATACTCTCAAGATATGACTATCAAAGGAAATGGGTTTGTTCCTAGTGCAAGAGTACAGGAATTAAGAAAGAAGCTGACTAATTTTATGGAGAAACATGTTTATCAATTGGAAAAGGAATTTATCAGGCATGCTCAATCAGACAAGCGGTGGACAATTCACCCGTCTGAAGAGACACACAAAGATATGGCAAAAGCACAAGGCCTTTGGAACCTCTGGGTACCAGTTGATAGTGCTGCCATGGTTAAGAAGTTTTCTGATGAAAGAAAATTCGATATTCATATCAAGGATAGGCATGAGAGCTTGTTGCTTGGGGCTGGTCTTTCCAATCTTGAATATTCATATCTTTGTGAAATCATGGGTCGCTCAGCTTGGGCTCCTCAAATTTTCAATTGCAGTGCACCAGACACAGGAAATATGGAGGTATTGTTTCGTTATGGCACTGTGGAACAACAAAGGACATGGCTCATTCCTCTATTGGAGGGGAAAATTAGATCTGGATTTGCAATGACAGAGCCTCAAGTTGCATCCTCCGATGCCACTAACATAGAATGCTCAATTTTACGTGAAGGAGATCATTATATCATTAATGGTCGGAAATGGTGGACAAGTGGAGCAATGGATCCAAGATGCAAGATTCTGATAGTTATGGGAAAAACAGATATGAATGCACCTAAGCATAAACAGCAATCAATGGTTCTGGTTCCTATTGATACTCCTGGTGTCCACATAGAAAGAGCCTTGACTGTTTTTGGTTTTGATGATGCTCCTCATGGCCATGCAGAGATTGTCTTTGAGAATGTGCAGGTTCCTATCAAAAATGTCATTCTGGGAGAAGGCCGTGGTTTTGAGATTGCACAAGGACGGTTGGGTCCAGGTCGATTACATCATTGTATGAGATTAGTTGGTGCTGCCGAACGTGGCATTGAAATGATGGTAAAAAGGGCACTCCAAAGAAAAGCCTTTGGCAAGTTGATCGCACAGCATGGATCATTCTTATCTGATCTTGCCAAGTGTCGTTTAGAAATAGAGAAAACAAGATTTTTGGTACTGGAAGCTGCAAATCAATTGGACTTGTTTGGCAATAAAGTTGCCAGAGGAGCAATTGCCATGGCTAAGGTTGCAGCTCCAAACATGGCTTTGAAAGTACTTGATACAGCAATCCAAGTTCATGGTGCCGCAGGTGTAAGCACAGACTTCGTACTTTCCCACCTCTGGGCTTCTGCTCGAACTTTGCGATTAGCAGATGGCCCTGATGAGGTCCATCTTGGGACTATAGCAAAACTTGAATTGTCTAGAGCAAGGCTCTGAGATCAAGTTAGTATGTTTTATCTAGAAAATCCATCAAAAGCATTCATCTCAACTAGATAACATTCTCAGAAAAAAGCAAAATTTGGGGAAGGATACTTACAGGAGGACATGAAGAATGTTAAATTCTATATTTTGGTATGTTCCAACTTGTAAGTTTATTTGAGAACTACCATAGGGTGAAGCAGGGTATAAAGTTTCCATGATCCAAAGGGAACTATAATTTGTAAATGAGAAAAAGCTATCTTTCGAAGTCACCAGCTGATTAACACCTTTGCTATTCAATTTAAATATTTAGTTGAATAAACTGATTCAAATGATTCTTTTTGGGAGACACTTAACAGTTTGTATAATTATGAATCTGATTGAAAATTACTTATTTATAGATGTAGTTTCATTCCTATATTGGATCAATCATGTTTTACATATTTTTCTCTGGAGCAGTGCGGGAATCAT

Ace file of the UniGene sp_v2.0_unigene215 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	acyl-CoA dehydrogenase [Arabidopsis thaliana] gb\|AAM14036.1\| unknown protein [Arabidopsis thaliana] gb\|AAN86198.1\| unknown protein [Arabidopsis thaliana] gb\|AEE74461.1\| acyl-CoA dehydrogenase [Arabidopsis thaliana]	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	25%
Blast2go	acyl- dehydrogenase	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Acyl-CoA dehydrogenase.	EC:1.3.99.3	-	0.0	78%

Source	KEGGs	Term	Identity
Blast2go	Fatty acid metabolism	00071	78%
Blast2go	Valine, leucine and isoleucine degradation	00280	78%
Blast2go	beta-Alanine metabolism	00410	78%
Blast2go	Propanoate metabolism	00640	78%
Blast2go	Metabolic pathways	01100	78%
Blast2go	Biosynthesis of secondary metabolites	01110	78%

Source	GOs	Term	Type	Identity
Blast2go	root hair elongation	GO:0048767	Biological Process	78%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	78%
Blast2go	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	78%
Blast2go	acyl-CoA dehydrogenase activity	GO:0003995	Molecular Function	78%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Nitrogenase component 1, conserved site	IPR000318	-	-
Sma3	Aminoglycoside phosphotransferase	IPR002575	-	-
Sma3	Acyl-CoA oxidase/dehydrogenase, type 1	IPR006090	-	-
Sma3	Acyl-CoA oxidase/dehydrogenase, central domain	IPR006091	-	-
Sma3	Acyl-CoA dehydrogenase, N-terminal	IPR006092	-	-
Sma3	Tyrosine-protein kinase, active site	IPR008266	-	-
Sma3		IPR013764	-	-
Sma3	Acyl-CoA dehydrogenase/oxidase, N-terminal	IPR013786	-	-
Sma3	ABC transporter, conserved site	IPR017871	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPF0

Fln msg: your sequence is longer than subject: 836 - 227

Fln protein: MVVQTSSMIGQVQQAHRFDESSLIRYAQANVEGFPLPVTKFKVSQFGHGQSNPTFFLEVESYTTVKQYVLRKKPPGKLLQSAHAIEREFEVLAALGKYSNVPVPKVFCFCTDTNVIGTTFYIMEYLEGRIFLNPGLPELNPKQRRAIYLATTKTLAAIHNVDVDAVGLGHYGRCENYCKRQVERWAKQYLASTGEGKPDRSPRMLELIDWLKQHIPADVVTLSSKTGLVHGDFRIDNLLFHPSEERVIGVLDWELSTLGDPMSDVAYNCMCFLINNNEFPVPHYGFDNGIMPEGVPSLEEHLANYCAAAGTQWPAKDWKFYIAFSLFRSASIFAGVYYRWLKGNASGGERAKSAGRLAIYFIDTAWKFISRDSVLPDRPPSSAFGNVPLETDVSLSHIPAYSQDMTIKGNGFVPSARVQELRKKLTNFMEKHVYQLEKEFIRHAQSDKRWTIHPSEETHKDMAKAQGLWNLWVPVDSAAMVKKFSDERKFDIHIKDRHESLLLGAGLSNLEYSYLCEIMGRSAWAPQIFNCSAPDTGNMEVLFRYGTVEQQRTWLIPLLEGKIRSGFAMTEPQVASSDATNIECSILREGDHYIINGRKWWTSGAMDPRCKILIVMGKTDMNAPKHKQQSMVLVPIDTPGVHIERALTVFGFDDAPHGHAEIVFENVQVPIKNVILGEGRGFEIAQGRLGPGRLHHCMRLVGAAERGIEMMVKRALQRKAFGKLIAQHGSFLSDLAKCRLEIEKTRFLVLEAANQLDLFGNKVARGAIAMAKVAAPNMALKVLDTAIQVHGAAGVSTDFVLSHLWASARTLRLADGPDEVHLGTIAKLELSRARL*

Protein Length: 837

Fln nts: CGTCGTGACCTTGGCTGTCAGTGGATATGTATCGTACCTACAGCAGGAGCTGAGAAATAATTGGTTACGCATCCCTTGCCTCATGAATTAATCCATGCTGTGTGTCACATGCTGCGTACATTATTTAGAGGGTTTCTGTGATCTGATCGTTGAACGATTTGAAGGTTTGTGTTGGGCTTTTGAGCTTATTCAGCATCACCAGGGTTACATTGAAAA_-_ATGGTAGTGCAAACTTCCAGTATGATTGGTCAGGTTCAACAAGCTCATCGATTTGATGAGAGCTCTCTTATTCGTTACGCTCAGGCAAATGTTGAAGGCTTCCCACTTCCTGTGACAAAATTCAAAGTTTCACAGTTTGGCCATGGACAATCAAATCCAACATTTTTTCTCGAAGTTGAGTCATATACAACCGTCAAACAATATGTTCTGCGAAAAAAGCCTCCTGGAAAGTTGCTCCAGTCTGCACATGCAATTGAAAGGGAATTTGAGGTGTTGGCAGCTTTAGGCAAATATTCAAATGTTCCAGTCCCAAAGGTTTTTTGTTTTTGTACTGATACAAATGTAATTGGTACAACCTTTTACATCATGGAGTATTTGGAAGGTCGGATTTTTCTGAATCCTGGACTGCCGGAACTCAACCCAAAACAAAGAAGAGCCATATACCTTGCGACTACCAAAACTTTGGCAGCAATACACAATGTGGATGTTGATGCGGTAGGATTAGGTCACTATGGACGCTGTGAAAATTACTGCAAGAGGCAGGTAGAAAGATGGGCAAAGCAATATCTAGCTTCAACTGGTGAAGGAAAACCAGATCGAAGTCCAAGGATGTTGGAACTCATTGATTGGCTCAAGCAACACATACCAGCTGACGTCGTGACCTTGAGCTCAAAGACTGGTCTTGTTCATGGAGACTTTCGGATTGATAATCTTCTGTTCCATCCATCAGAGGAACGTGTCATTGGTGTTTTAGATTGGGAATTATCAACATTGGGGGACCCAATGTCTGATGTTGCTTACAACTGCATGTGTTTTCTCATCAACAACAATGAGTTTCCAGTTCCTCATTATGGCTTTGATAATGGGATAATGCCAGAAGGAGTTCCTTCTCTGGAAGAGCATCTGGCGAATTATTGTGCTGCAGCTGGTACCCAGTGGCCTGCTAAAGATTGGAAGTTTTATATTGCATTCTCTCTTTTTCGTTCAGCATCTATTTTTGCGGGGGTTTATTATAGGTGGTTGAAGGGAAATGCCTCAGGGGGGGAACGTGCCAAGTCTGCTGGACGCTTAGCTATATATTTTATTGATACAGCTTGGAAGTTTATAAGTAGGGATTCAGTACTTCCAGACAGGCCACCATCTTCTGCTTTTGGAAATGTACCATTGGAAACAGATGTCTCTTTATCCCATATTCCAGCATACTCTCAAGATATGACTATCAAAGGAAATGGGTTTGTTCCTAGTGCAAGAGTACAGGAATTAAGAAAGAAGCTGACTAATTTTATGGAGAAACATGTTTATCAATTGGAAAAGGAATTTATCAGGCATGCTCAATCAGACAAGCGGTGGACAATTCACCCGTCTGAAGAGACACACAAAGATATGGCAAAAGCACAAGGCCTTTGGAACCTCTGGGTACCAGTTGATAGTGCTGCCATGGTTAAGAAGTTTTCTGATGAAAGAAAATTCGATATTCATATCAAGGATAGGCATGAGAGCTTGTTGCTTGGGGCTGGTCTTTCCAATCTTGAATATTCATATCTTTGTGAAATCATGGGTCGCTCAGCTTGGGCTCCTCAAATTTTCAATTGCAGTGCACCAGACACAGGAAATATGGAGGTATTGTTTCGTTATGGCACTGTGGAACAACAAAGGACATGGCTCATTCCTCTATTGGAGGGGAAAATTAGATCTGGATTTGCAATGACAGAGCCTCAAGTTGCATCCTCCGATGCCACTAACATAGAATGCTCAATTTTACGTGAAGGAGATCATTATATCATTAATGGTCGGAAATGGTGGACAAGTGGAGCAATGGATCCAAGATGCAAGATTCTGATAGTTATGGGAAAAACAGATATGAATGCACCTAAGCATAAACAGCAATCAATGGTTCTGGTTCCTATTGATACTCCTGGTGTCCACATAGAAAGAGCCTTGACTGTTTTTGGTTTTGATGATGCTCCTCATGGCCATGCAGAGATTGTCTTTGAGAATGTGCAGGTTCCTATCAAAAATGTCATTCTGGGAGAAGGCCGTGGTTTTGAGATTGCACAAGGACGGTTGGGTCCAGGTCGATTACATCATTGTATGAGATTAGTTGGTGCTGCCGAACGTGGCATTGAAATGATGGTAAAAAGGGCACTCCAAAGAAAAGCCTTTGGCAAGTTGATCGCACAGCATGGATCATTCTTATCTGATCTTGCCAAGTGTCGTTTAGAAATAGAGAAAACAAGATTTTTGGTACTGGAAGCTGCAAATCAATTGGACTTGTTTGGCAATAAAGTTGCCAGAGGAGCAATTGCCATGGCTAAGGTTGCAGCTCCAAACATGGCTTTGAAAGTACTTGATACAGCAATCCAAGTTCATGGTGCCGCAGGTGTAAGCACAGACTTCGTACTTTCCCACCTCTGGGCTTCTGCTCGAACTTTGCGATTAGCAGATGGCCCTGATGAGGTCCATCTTGGGACTATAGCAAAACTTGAATTGTCTAGAGCAAGGCTCTGA___GATCAAGTTAGTATGTTTTATCTAGAAAATCCATCAAAAGCATTCATCTCAACTAGATAACATTCTCAGAAAAAAGCAAAATTTGGGGAAGGATACTTACAGGAGGACATGAAGAATGTTAAATTCTATATTTTGGTATGTTCCAACTTGTAAGTTTATTTGAGAACTACCATAGGGTGAAGCAGGGTATAAAGTTTCCATGATCCAAAGGGAACTATAATTTGTAAATGAGAAAAAGCTATCTTTCGAAGTCACCAGCTGATTAACACCTTTGCTATTCAATTTAAATATTTAGTTGAATAAACTGATTCAAATGATTCTTTTTGGGAGACACTTAACAGTTTGTATAATTATGAATCTGATTGAAAATTACTTATTTATAGATGTAGTTTCATTCCTATATTGGATCAATCATGTTTTACATATTTTTCTCTGGAGCAGTGCGGGAATCAT

Fln Alignment:

Contig215___WWTSGAMDPRCKILIVMGKTDMNAPKHKQQSMVLVPIDTPGVHIERALTVFGFDDAPHGHAEIVFENVQVPIKNVILGEGRGFEIAQGRLGPGRLHHCMRLVGAAERGIEMMVKRALQRKAFGKLIAQHGSFLSDLAKCRLEIEKTRFLVLEAANQLD--LFGNKVARGAIAMAKVAAPNMALKVLDTAIQVHGAAGVSTDFVLSHLWASARTLRLADGPDEV
B8LPF0_____________WCTNG---PVAETLIIYAKTDPNAGS-KGITTFIIEKGMEGFSTAQKLDKLGMRGSDT--CELVFENCFVPEENILGKEGKGVYVLMSGLDLERLVLSAGPIGLMQASMDVVLPYVRQREQFGRPIGEFQLIQGKLADMYTALQSSRAFVYSVAKKCDNGEVDKKDCAGVILFAAENATQVALQ----AIQCLGGNGYVNEYPTGRLLRDAKLFEIGAGTSEI

SNPs (tot: 101)

Position	A %	C %	G %	T %	Probability
866		66		33	0.997452
1011		88	11		0.999952
1035		18		81	1.0
1036	18		81		1.0
1038			81	18	1.0
1130	10			90	0.90298
1131		10		90	0.90298
1132		10	90		0.90298
1273	88		11		0.923564
1275			12	87	0.992554
1277			12	87	0.992554
1278	12			87	0.992554
1280	12	87			0.992554
1281		87	12		0.992554
1283		25		75	1.0
1284	12	87			0.992554
1285	12	87			0.992554
1286	87		12		0.992554
1287	87		12		0.992554
1292		12	87		0.992554
1348	87	12			0.992554
1349		12		87	0.992554
1350	12			87	0.992554
1548	83		16		0.994512
1576		66		33	1.0
1606	85	14			0.993533
1608			14	85	0.993533
1813	66		33		1.0
1817	66			33	1.0
1823	66	33			1.0
1824		33		66	1.0
1825	33		66		1.0
1826	50		50		1.0
1827			33	66	1.0
1829			66	33	1.0
1831	33	66			1.0
1832		33		66	1.0
1834	33			66	1.0
1975	75		25		0.996473
1978		75	25		0.996473
2000	75		25		0.996473
2034			87	12	0.992554
2042		12		87	0.992554
2112		87		12	0.992554
2116	87			12	0.992554
2132		85		14	0.993533
2169	16		83		0.994512
2216	88			11	0.923564
2217	88		11		0.923564
2221		88	11		0.923564
2226			11	88	0.923564
2228	88			11	0.923564
2234	11			88	0.923564
2236		88	11		0.923564
2288		83		16	0.994512
2289		83	16		0.994512
2290	66			33	1.0
2292	33			50	1.0
2293		50	33		1.0
2295			50	50	1.0
2296	50			50	1.0
2297		50	50		1.0
2299		40	60		1.0
2302		60		20	0.99255
2304	60			40	1.0
2305			60	40	1.0
2306			60	40	1.0
2308	40	60			1.0
2310	40	60			1.0
2312	60			40	1.0
2314			60	40	1.0
2633		25		75	0.996473
3086		88		11	0.923564
3087	88		11		0.923564
3090		88	11		0.923564
3091	88			11	0.923564
3095		11		88	0.923564
3096	11		88		0.923564
3098	88	11			0.923564
3099		11		88	0.923564
3100	11			88	0.923564
3102	88		11		0.923564
3103			11	88	0.923564
3107		11		88	0.923564
3110		11	88		0.923564
3128	87	12			0.992554
3129	87	12			0.992554
3130		14		85	0.993533
3131		14		42	1.0
3132	66		33		1.0
3133	33			66	1.0
3134			40	60	1.0
3136		60	40		1.0
3137	40			60	1.0
3144	40	60			1.0
3145	40			60	1.0
3147		60	40		1.0
3149		40	60		1.0
3152		40	60		1.0
3155	80	20			0.995493
3157		20	80		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain