SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene199

Length: 2995 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene199
CGTCGTGACCTTGGCTGTCACTCAATTGTAAATTAGATGAAATGGCTGGAAGCTGAGGGCTAACAATGGAGAAAGACGCAGACACAAAATAGTCAACCGAGCTTTCGCAGGAGGATTCAACCGAGATATCACTAGAGGTGGCCTCCGAATGGAAGGAAGTCGCGGATAAGGAATTTGAAGGGAGGCAGTCACCGATTCAGTGAGTCCCTTACGTCAATGACAGGATAGACATAAAAACCAAGAAGCTACTCACTTTGAACTAACTGGATGAGGATCTGGGATTACAGGGACAGATAGAAGTCATAACTGCAATCTGAGTGATTCTGTTGAAACTCTGGATTGTGCATTGAAGGATTGGCAATGTGGAAGCTGAAGATTGCTGAAGGGGGAAGCCCCTGGTTGCAAACTTTGAACAATCATGTTGGAAGACAAGTCTGGGAATTTGATCTAAAAGCTGGTACATCTGAGGATCACTTTGCCGTGGAAAAAGCTCGTGCAGATTTTAATAACGAGAGATTTATCCAACACCACAGTGCTGATTTTCTTATGAGGTTGCAGTTTGCGAGAGAAAATCCTCTCTCTCCTCTTCCACCCCAAGTTAAAATAGAAAGTGCTAATGACATCACTGAGGAGGCAATACAAACTACATTGACGCGAGCAGTACGCTTTTATGCTACTATTCAAGCACATGATGGACACTGGCCTGGTGATTATGGTGGCCCATTGTTCCTTATGCCAGGTTTGGTTATTGTCTTGTATGTGACAGGAGCATTAAATGCAGTTTTATCAGAAATGCATAAAAAGGAAATTTGTCGATATTTATATAACCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGTCACAGCACAATGTTTGGAACAGTGTTAAACTATGTTACTTTGAGACTTCTTGGACAAGCACCTGATGGAGGACAAGGAACCATGGAAAAAGGGCGTCAATGGATTTTGGATCATGGTGGTGCCACTGCAATACCTTCCTGGGGAAAAATGTGGCTTTCAGTACTTGGAGTATTTGATTGGATTGGAAACAATCCATTGCCCCCAGAAATTTGGCTTCTCCCATATTTTCTTCCAATCCATCCAGGGAGAATGTGGTGTCACTGTCGAATGGTTTATTTGCCAATGTCATATCTGTATGGGCAGAGATTTGTGGGTCCTCTAACTGGAATTGTTATGTCTTTAAGAGAGGAGCTTTATACAGTGCCTTACGAGAAAATAGATTGGAATCAAGCACGTAACACGTGTGCAAAGGAAGATCTTTACTATCCACATCCTTTAATACAAGATATCCTATGGGGAACACTTCATAAAGTAGTAGAGCCTGCACTTATGCATTGGCCAGGCTCAATGCTGCGAGAGAGGGCACTTCAGTCAGTGATGAAGCATGTACACTATGAGGATGAAAATACTCGCTACATCTGCATTGGCCCTGTTAACAAGGTATTGAATATGCTTTGTTGCTGGGTAGAAGATTCAAACTCGGAGGCATTTAAAAGGCATCTTCCACGAATATTAGACTATCTATGGGTTGCAGAAGATGGCATGAAGATGCAGGGTTACAATGGCAGTCAGTTGTGGGATACAGCGTTTGCTACCCAAGCTCTTATATCAACTGACCTCCTTGATGATTGTGGTCCAATGCTTAAGAAGGCACATATGTATATAGAAAGGTCACAGGTTATAGCAGATTGTCCAGGTGATTTGAATTTCTGGTATCGTCATATTTCCAAGGGTGCCTGGCCTTTCTCAACTAGGGATCATGGTTGGCCAATTTCAGATTGCTCTTCTGAAGGCCTAAAGGCTGCGTTAGCACTGTCACAATTGCCTCAAGATATTGTGGGGAAACCAATTCCCTCGCAACGATTTTTTGATTGTGTCAGTTATATGCTTTCCATGCAGAATAATGATGGTGGATTTGCAACTTATGAATTGACAAGATCCTATCCTTGGTTAGAGATAATCAATCCTGCAGAGACATTTGGAGATATTGTCATTGACTATTCGTATGTGGAGTGCACTTCAGCAGTAATTCAAGCTCTTGCAGCATTTAAGAAACTGTATCCAGAGCATCAATGTAAAGAAATAGAAACATGCATTTTAAAGGCAACTCAGTACATTGAAAACATCCAGAGGCCTGATGGTTCATGGTATGGCTCATGGGGAGTCTGCTTCACATATGGTACATGGTTTGGTGTTCTGGGGTTAGTGGCAGCTGGCAAAACCTACCAGAATTGTTCAAATATTCGTAATGCTTGCGAGTTCCTTCTTTCAAAGCAGCTTGCTTCTGGTGGTTGGGGAGAAAGTTATCTTTCATGCCAAGATAAGGTCTATACACACTTGGAGGGTGGAAGATCGCACATAGTAAACACAGCATGGGCTATGCTGGCTCTTATTTATGCAGGCCAGGCTTTGAGGGATCCAAAACCTCTTCATCGAGCAGCAATTGTACTTATCAATTCGCAGATGGAGAATGGTGATTTTCCACAACAGGAAATTATGGGAGTTTTCAATAGGAATTGTATGATCTCCTATTCTGCTTATCGGAATATCTTTCCTATATGGGCTCTAGGAGAATATCGCCGTCATGTTCTGCAATCATAATGTATATATTTCTCTTATCTTTTAAAGGTCAGCTGTTGGGGATAAACCTTACTGGTATTTTTGCGGTAGGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCAAGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCGAGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCAAGTTTGCATGTATGAAAAACTTTGTAATCATGGAATGGTACTCAATTTTCTAGTTAGACTTTAGAATACAATAGAACCGA

Ace file of the UniGene sp_v2.0_unigene199 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cycloartenol synthase n=2 Tax=Pinaceae RepID=Q9FQM1_ABIMA	-	-	92%
FL-Next	tr=Cycloartenol synthase; Abies magnifica (Red fir).	-	-	92%
Blast2go	cycloartenol synthase	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Cycloartenol synthase.	EC:5.4.99.8	-	0.0	86%

Source	KEGGs	Term	Identity
Blast2go	Steroid biosynthesis	00100	86%
Blast2go	Biosynthesis of terpenoids and steroids	01062	86%
Blast2go	Biosynthesis of plant hormones	01070	86%
Blast2go	Metabolic pathways	01100	86%
Blast2go	Biosynthesis of secondary metabolites	01110	86%

Source	GOs	Term	Type	Identity
Blast2go	thylakoid membrane organization	GO:0010027	Biological Process	86%
Blast2go	pollen development	GO:0009555	Biological Process	86%
Blast2go	negative regulation of transcription, DNA-dependent	GO:0045892	Biological Process	86%
Blast2go	pentacyclic triterpenoid biosynthetic process	GO:0019745	Biological Process	86%
Blast2go	cycloartenol synthase activity	GO:0016871	Molecular Function	86%
Blast2go	vacuole	GO:0005773	Cellular Component	86%

Source	InterPros	Term	Type	Identity
Sma3	Prenyltransferase/squalene oxidase	IPR001330	-	-
Sma3	Squalene cyclase	IPR018333	-	-
Sma3	Terpene synthase, conserved site	IPR002365	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: Q9FQM1

Fln msg:

Fln protein: MWKLKIAEGGSPWLQTLNNHVGRQVWEFDLKAGTSEDHFAVEKARADFNNERFIQHHSADFLMRLQFARENPLSPLPPQVKIESANDITEEAIQTTLTRAVRFYATIQAHDGHWPGDYGGPLFLMPGLVIVLYVTGALNAVLSEMHKKEICRYLYNHQNEDGGWGLHIEGHSTMFGTVLNYVTLRLLGQAPDGGQGTMEKGRQWILDHGGATAIPSWGKMWLSVLGVFDWIGNNPLPPEIWLLPYFLPIHPGRMWCHCRMVYLPMSYLYGQRFVGPLTGIVMSLREELYTVPYEKIDWNQARNTCAKEDLYYPHPLIQDILWGTLHKVVEPALMHWPGSMLRERALQSVMKHVHYEDENTRYICIGPVNKVLNMLCCWVEDSNSEAFKRHLPRILDYLWVAEDGMKMQGYNGSQLWDTAFATQALISTDLLDDCGPMLKKAHMYIERSQVIADCPGDLNFWYRHISKGAWPFSTRDHGWPISDCSSEGLKAALALSQLPQDIVGKPIPSQRFFDCVSYMLSMQNNDGGFATYELTRSYPWLEIINPAETFGDIVIDYSYVECTSAVIQALAAFKKLYPEHQCKEIETCILKATQYIENIQRPDGSWYGSWGVCFTYGTWFGVLGLVAAGKTYQNCSNIRNACEFLLSKQLASGGWGESYLSCQDKVYTHLEGGRSHIVNTAWAMLALIYAGQALRDPKPLHRAAIVLINSQMENGDFPQQEIMGVFNRNCMISYSAYRNIFPIWALGEYRRHVLQS*

Protein Length: 758

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTGTAAATTAGATGAAATGGCTGGAAGCTGAGGGCTAACAATGGAGAAAGACGCAGACACAAAATAGTCAACCGAGCTTTCGCAGGAGGATTCAACCGAGATATCACTAGAGGTGGCCTCCGAATGGAAGGAAGTCGCGGATAAGGAATTTGAAGGGAGGCAGTCACCGATTCAGTGAGTCCCTTACGTCAATGACAGGATAGACATAAAAACCAAGAAGCTACTCACTTTGAACTAACTGGATGAGGATCTGGGATTACAGGGACAGATAGAAGTCATAACTGCAATCTGAGTGATTCTGTTGAAACTCTGGATTGTGCATTGAAGGATTGGCA_-_ATGTGGAAGCTGAAGATTGCTGAAGGGGGAAGCCCCTGGTTGCAAACTTTGAACAATCATGTTGGAAGACAAGTCTGGGAATTTGATCTAAAAGCTGGTACATCTGAGGATCACTTTGCCGTGGAAAAAGCTCGTGCAGATTTTAATAACGAGAGATTTATCCAACACCACAGTGCTGATTTTCTTATGAGGTTGCAGTTTGCGAGAGAAAATCCTCTCTCTCCTCTTCCACCCCAAGTTAAAATAGAAAGTGCTAATGACATCACTGAGGAGGCAATACAAACTACATTGACGCGAGCAGTACGCTTTTATGCTACTATTCAAGCACATGATGGACACTGGCCTGGTGATTATGGTGGCCCATTGTTCCTTATGCCAGGTTTGGTTATTGTCTTGTATGTGACAGGAGCATTAAATGCAGTTTTATCAGAAATGCATAAAAAGGAAATTTGTCGATATTTATATAACCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGTCACAGCACAATGTTTGGAACAGTGTTAAACTATGTTACTTTGAGACTTCTTGGACAAGCACCTGATGGAGGACAAGGAACCATGGAAAAAGGGCGTCAATGGATTTTGGATCATGGTGGTGCCACTGCAATACCTTCCTGGGGAAAAATGTGGCTTTCAGTACTTGGAGTATTTGATTGGATTGGAAACAATCCATTGCCCCCAGAAATTTGGCTTCTCCCATATTTTCTTCCAATCCATCCAGGGAGAATGTGGTGTCACTGTCGAATGGTTTATTTGCCAATGTCATATCTGTATGGGCAGAGATTTGTGGGTCCTCTAACTGGAATTGTTATGTCTTTAAGAGAGGAGCTTTATACAGTGCCTTACGAGAAAATAGATTGGAATCAAGCACGTAACACGTGTGCAAAGGAAGATCTTTACTATCCACATCCTTTAATACAAGATATCCTATGGGGAACACTTCATAAAGTAGTAGAGCCTGCACTTATGCATTGGCCAGGCTCAATGCTGCGAGAGAGGGCACTTCAGTCAGTGATGAAGCATGTACACTATGAGGATGAAAATACTCGCTACATCTGCATTGGCCCTGTTAACAAGGTATTGAATATGCTTTGTTGCTGGGTAGAAGATTCAAACTCGGAGGCATTTAAAAGGCATCTTCCACGAATATTAGACTATCTATGGGTTGCAGAAGATGGCATGAAGATGCAGGGTTACAATGGCAGTCAGTTGTGGGATACAGCGTTTGCTACCCAAGCTCTTATATCAACTGACCTCCTTGATGATTGTGGTCCAATGCTTAAGAAGGCACATATGTATATAGAAAGGTCACAGGTTATAGCAGATTGTCCAGGTGATTTGAATTTCTGGTATCGTCATATTTCCAAGGGTGCCTGGCCTTTCTCAACTAGGGATCATGGTTGGCCAATTTCAGATTGCTCTTCTGAAGGCCTAAAGGCTGCGTTAGCACTGTCACAATTGCCTCAAGATATTGTGGGGAAACCAATTCCCTCGCAACGATTTTTTGATTGTGTCAGTTATATGCTTTCCATGCAGAATAATGATGGTGGATTTGCAACTTATGAATTGACAAGATCCTATCCTTGGTTAGAGATAATCAATCCTGCAGAGACATTTGGAGATATTGTCATTGACTATTCGTATGTGGAGTGCACTTCAGCAGTAATTCAAGCTCTTGCAGCATTTAAGAAACTGTATCCAGAGCATCAATGTAAAGAAATAGAAACATGCATTTTAAAGGCAACTCAGTACATTGAAAACATCCAGAGGCCTGATGGTTCATGGTATGGCTCATGGGGAGTCTGCTTCACATATGGTACATGGTTTGGTGTTCTGGGGTTAGTGGCAGCTGGCAAAACCTACCAGAATTGTTCAAATATTCGTAATGCTTGCGAGTTCCTTCTTTCAAAGCAGCTTGCTTCTGGTGGTTGGGGAGAAAGTTATCTTTCATGCCAAGATAAGGTCTATACACACTTGGAGGGTGGAAGATCGCACATAGTAAACACAGCATGGGCTATGCTGGCTCTTATTTATGCAGGCCAGGCTTTGAGGGATCCAAAACCTCTTCATCGAGCAGCAATTGTACTTATCAATTCGCAGATGGAGAATGGTGATTTTCCACAACAGGAAATTATGGGAGTTTTCAATAGGAATTGTATGATCTCCTATTCTGCTTATCGGAATATCTTTCCTATATGGGCTCTAGGAGAATATCGCCGTCATGTTCTGCAATCATAA___TGTATATATTTCTCTTATCTTTTAAAGGTCAGCTGTTGGGGATAAACCTTACTGGTATTTTTGCGGTAGGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCAAGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCGAGTAGGATTGATATCTATGGTTCTGCAATGAAGATGACAAAATAATTTTCAAACTTTGTTGACTGCGATTCAAGTTTGCATGTATGAAAAACTTTGTAATCATGGAATGGTACTCAATTTTCTAGTTAGACTTTAGAATACAATAGAACCGA

Fln Alignment:

Contig199___MWKLKIAEGGSPWLQTLNNHVGRQVWEFDLKAGTSEDHFAVEKARADFNNERFIQHHSADFLMRLQFARENPLSPLPPQVKIESANDITEEAIQTTLTRAVRFYATIQAHDGHWPGDYGGPLFLMPGLVIVLYVTGALNAVLSEMHKKEICRYLYNHQNEDGGWGLHIEGHSTMFGTVLNYVTLRLLGQAPDGGQGTMEKGRQWILDHGGATAIPSWGKMWLSVLGVFDWIGNNPLPPEIWLLPYFLPIHPGRMWCHCRMVYLPMSYLYGQRFVGPLTGIVMSLREELYTVPYEKIDWNQARNTCAKEDLYYPHPLIQDILWGTLHKVVEPALMHWPGSMLRERALQSVMKHVHYEDENTRYICIGPVNKVLNMLCCWVEDSNSEAFKRHLPRILDYLWVAEDGMKMQGYNGSQLWDTAFATQALISTDLLDDCGPMLKKAHMYIERSQVIADCPGDLNFWYRHISKGAWPFSTRDHGWPISDCSSEGLKAALALSQLPQDIVGKPIPSQRFFDCVSYMLSMQNNDGGFATYELTRSYPWLEIINPAETFGDIVIDYSYVECTSAVIQALAAFKKLYPEHQCKEIETCILKATQYIENIQRPDGSWYGSWGVCFTYGTWFGVLGLVAAGKTYQNCSNIRNACEFLLSKQLASGGWGESYLSCQDKVYTHLEGGRSHIVNTAWAMLALIYAGQALRDPKPLHRAAIVLINSQMENGDFPQQEIMGVFNRNCMISYSAYRNIFPIWALGEYRRHVLQS
Q9FQM1_____________MWKLKIAEGGSPWLQTLNNHVGRQVWEFDPKAGTSEDHLAVEKARVDFYNKRFIQHHSADLLMRLQCGGENPLSPLPAQVKLESANDITEEVIQTTLVRAIRFYATIQAHDGHWPGDYGGPMFLMPGLVIALYVTGALNAVLSEMHKKEICRYLYNHQNEDGGWGLHIEGHSTMFGTVLNYVTLRLLGQAPDGGQGAMEKGCAWILDHGGATAIPSWGKMWLSVLGVFDWTGNNPLPPEMWLLPYFLPTHPGRMWCHCRMVYLPMSYIYGRRFVGPLTGIVMSLREELYTVPYEKIDWNQARSMCAKEDLYYPHPFLQDILWGTLHKVVEPALMHWPGSMLRERALQSVMKHIHYEDENTRYICIGPVNKVLNMLCCWVEDSNSEAFKRHLARVVDYLWVAEDGMKMQGYNGSQLWDTAFATQALISTNLLDDCGPLLKKAHIYIERSQVQEDCPGDLNFWYRHISNGAWPFSTRDHGWPISDCSSEGLKAALALSQLPQDIVGKPIPSQRIFDCVNLMLSMQNSDGGFATYELTRSYPWLEKINPAETFGDIVIDYSYVECTSAITQALVSFKKLYPEHRHKEIETCILKATRYIENIQRPDGSWYGSWGVCFTYGTWFGVLGLAAAGKTYQNCSNIRKACEFLLSKQLPSGGWGESYLSCQEKVYTHLEGGRSHIVSTAWAMLALIYAGQALRDPKPLHRAAIVLVNYQMENGDFPQQEIMGVFNRNCMISYSAYRNIFPIWALGEYCRHVLQS

SNPs (tot: 45)

Position	A %	C %	G %	T %	Probability
2312		80		20	0.995493
2563	90	10			0.90298
2565		10		90	0.90298
2567	10		90		0.90298
2588	88	11			0.923564
2590			88	11	0.923564
2601			85	14	0.993533
2606	16			83	0.994512
2607			16	83	0.994512
2610	16	83			0.994512
2611	16	83			0.994512
2612		66	16		0.988813
2613			16	83	0.994512
2617		11	88		0.923564
2618		88	11		0.923564
2620	88	11			0.923564
2621			11	88	0.923564
2701		12		87	0.992554
2707	14			85	0.993533
2708		85	14		0.993533
2713	14			85	0.993533
2714	85	14			0.993533
2716			14	85	0.993533
2719	14	85			0.993533
2722	85		14		0.993533
2725	14	85			0.993533
2726		14		85	0.993533
2843			11	88	0.999952
2906			15	84	0.999986
2908	53			23	1.0
2911			15	84	0.999986
2912	15			84	0.999986
2913		15	76	7	0.999983
2914	15			84	0.999986
2916		15		84	0.999986
2918	16			83	0.999991
2919	16		83		0.999991
2920		83	16		0.999991
2924	25			75	1.0
2925		16		83	0.999991
2926			16	83	0.999991
2928			16	83	0.999991
2955	71	28			1.0
2956		28		71	1.0
2957		14		71	0.984337

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain