SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene164

Length: 1995 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene164
TTAACAAAAAGCTTCCCTTAAAATAACAGACCAGACCGGACCGGACCGGACCAGACCAGACCACAACGCAATTAACTACGGTTACAGTTTTTCAGTTGAACGTCTGGCACGTACAGCGTGAGATCCTCTTAATGTTATGCACGTTCATCGCGCTTTACATCCAAGAATGTGCGGTGTGCCTTTTTAAAGGGAACAGAGGCAGGGTCTGGAGGGGTTAGAGCATACGCTTACTATTATATTTCGTCAGGCTTTGATCGGTTTCTTGAACGCCGTGTTTTCCACAGAAACAGAGTTAGCCGCCCGTAGCCTGCAAAAATGCTTATAACAGTGAAGAGTTCACAAATGGTGCGTCCCGCCGCACCGACTCCACAGAGGGATCTTTGGAACTCAAATGTAGACCTTGTGGTTCCGAGAATACATACCGCCAGTGTGTATTTCTATAGGCCCACTGGCAGCCCCGATTTCTTTTCAATGAACATTCTCAGGGATGCGCTTAGTAAACTGCTGGTGCCATTTTATCCCATGGCAGGGAGACTAAAAAGAGACCCAGATGGACGTATCGAAATCAACTGTAACGGTGAGGGTGTCCTGCTTGTTGAAGCTATAACAGATTCTGTCATTGATGATTTCGGGGATTTTGCGCCCACCATGGAGCTCAAACAACTCATTCCTAAAGTCAATTACTCAGAAGACATTTCTTCCTATCCTCTGCTCGTCCTTCAGGTAACCTTTTTCAAATGTGGAGGGGTGTCATTAGGTGTGGGGATGCAGCACCATGTGGCTGATGGTTATGCAGGTATACACTTCATCAATACCTGGTCTGATGTAGCCCGTGGGCTAGACATTACTCTGCCACCCTTCATCGACAGAACTCTTTTACGAGCCAGAAATCCTCCAACCCCGAAGTTTCAGCACATAGAGTACCAACAACCTCCACCATTGAAGGACACGAGTGGAATAATGAATGGTGAAAAAAATGGTTTCTCAGTTGCTATTTTTAAACTCACAAAAGAACAATTGGAGATCCTCAAGGGCAAAGCCAGAGAGAATGGAAATAACATAGCATACAGCAGCTATGAAATGCTCTCAGGACACATTTGGAGATGTGCCTGTAAAGCTAGAAATCTTGCTGAAGATCAAGAAACCAAATTGTATATTGCTACGGATGGTCGTAACCGGCTCAGACCATCCATTCCCCCTGGTTATTTTGGGAATGTTATTTTTACAACTACTCCCATGGCCGTTGCAGGGGACATTATATCAAAGCCTACATATTATGCTGCGAGTGTAATTCATGAAGCTTTGGGCCGGATGGATGATGAATACTTGCGTTCTGCTTTAGACTATTTGGAATTGCAGCCTGATTTGACTGCTCTAGTCAGAGGTTCTCATACTTTCAGGTGTCCAAATATTGGTATCACAAGCTGGTCTCGGTTGCCTATTCATGATGCAGACTTTGGGTGGGGAAGACCCATATTCATGGGACCTGGAGGTATTGCCTATGAAGGTCTTGCTTTCGTTTTGCCCAGTTCTGCCAATGATGGAAGTTTGTCAGTTGCATTGGGCTTGCAACCAGATCATATGGCTCGTTTTGCCAAAATGTTGTATGAAATCTAGATTTGACTTCTGTATAGGATTCAGGAACTAGAAATCGAAAAACCAGATTACATTATTTGTTTGGAACTGTAATTAGATTGAAGTACAACAATTTTGCCTTCTGTGAAAATTGAATCTGCTCAAAAATAAGATAAACAAACATGGAGAATTGTACTCGGGATCAAAATAAAAGATGACGCGAACTTGTTATTATCATCTTGATGTTGAAGTTGGAATCCGTTTAACCTGCAACTTCTCTATGATGTCAATGCATCTTGCCCCGGAATGATGCTTTAAGCTTTTCTTTGTACTCAGATTACAGAACTTTTGGCAAGTTCTTTTTCCCATACAGTATATATGCATGAACAATCTATAGAACCAGACTATATATTCTTGCTC

Ace file of the UniGene sp_v2.0_unigene164 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase n=2 Tax=Pinaceae RepID=A6Y879_PINRA	-	-	98%
FL-Next	tr=Hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase; Pinus radiata (Monterey pine) (Pinus insignis).	-	-	98%
Blast2go	hydroxycinnamoyl shikimate quinate hydroxycinnamoyltransferase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Shikimate O-hydroxycinnamoyltransferase.	EC:2.3.1.133	-	0.0	87%

Source	KEGGs	Term	Type	Identity
Blast2go	Phenylpropanoid biosynthesis	00940		87%
Blast2go	Flavonoid biosynthesis	00941		87%
Blast2go	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945		87%
Blast2go	Biosynthesis of phenylpropanoids	01061		87%
Blast2go	Biosynthesis of secondary metabolites	01110		87%
Blast2go	Anthranilate N-benzoyltransferase.	EC:2.3.1.144	-	87%

Source	GOs	Term	Type	e value	Identity
Blast2go	shikimate O-hydroxycinnamoyltransferase activity	GO:0047172	Molecular Function	0.0	87%
Blast2go	anthranilate N-benzoyltransferase activity	GO:0047672	Molecular Function	0.0	87%

Source	InterPros	Term	Type	e value	Identity
Sma3	Transferase	IPR003480	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A6Y879

Fln msg:

Fln protein: MLITVKSSQMVRPAAPTPQRDLWNSNVDLVVPRIHTASVYFYRPTGSPDFFSMNILRDALSKLLVPFYPMAGRLKRDPDGRIEINCNGEGVLLVEAITDSVIDDFGDFAPTMELKQLIPKVNYSEDISSYPLLVLQVTFFKCGGVSLGVGMQHHVADGYAGIHFINTWSDVARGLDITLPPFIDRTLLRARNPPTPKFQHIEYQQPPPLKDTSGIMNGEKNGFSVAIFKLTKEQLEILKGKARENGNNIAYSSYEMLSGHIWRCACKARNLAEDQETKLYIATDGRNRLRPSIPPGYFGNVIFTTTPMAVAGDIISKPTYYAASVIHEALGRMDDEYLRSALDYLELQPDLTALVRGSHTFRCPNIGITSWSRLPIHDADFGWGRPIFMGPGGIAYEGLAFVLPSSANDGSLSVALGLQPDHMARFAKMLYEI*

Protein Length: 435

Fln nts: TTAACAAAAAGCTTCCCTTAAAATAACAGACCAGACCGGACCGGACCGGACCAGACCAGACCACAACGCAATTAACTACGGTTACAGTTTTTCAGTTGAACGTCTGGCACGTACAGCGTGAGATCCTCTTAATGTTATGCACGTTCATCGCGCTTTACATCCAAGAATGTGCGGTGTGCCTTTTTAAAGGGAACAGAGGCAGGGTCTGGAGGGGTTAGAGCATACGCTTACTATTATATTTCGTCAGGCTTTGATCGGTTTCTTGAACGCCGTGTTTTCCACAGAAACAGAGTTAGCCGCCCGTAGCCTGCAAAA_-_ATGCTTATAACAGTGAAGAGTTCACAAATGGTGCGTCCCGCCGCACCGACTCCACAGAGGGATCTTTGGAACTCAAATGTAGACCTTGTGGTTCCGAGAATACATACCGCCAGTGTGTATTTCTATAGGCCCACTGGCAGCCCCGATTTCTTTTCAATGAACATTCTCAGGGATGCGCTTAGTAAACTGCTGGTGCCATTTTATCCCATGGCAGGGAGACTAAAAAGAGACCCAGATGGACGTATCGAAATCAACTGTAACGGTGAGGGTGTCCTGCTTGTTGAAGCTATAACAGATTCTGTCATTGATGATTTCGGGGATTTTGCGCCCACCATGGAGCTCAAACAACTCATTCCTAAAGTCAATTACTCAGAAGACATTTCTTCCTATCCTCTGCTCGTCCTTCAGGTAACCTTTTTCAAATGTGGAGGGGTGTCATTAGGTGTGGGGATGCAGCACCATGTGGCTGATGGTTATGCAGGTATACACTTCATCAATACCTGGTCTGATGTAGCCCGTGGGCTAGACATTACTCTGCCACCCTTCATCGACAGAACTCTTTTACGAGCCAGAAATCCTCCAACCCCGAAGTTTCAGCACATAGAGTACCAACAACCTCCACCATTGAAGGACACGAGTGGAATAATGAATGGTGAAAAAAATGGTTTCTCAGTTGCTATTTTTAAACTCACAAAAGAACAATTGGAGATCCTCAAGGGCAAAGCCAGAGAGAATGGAAATAACATAGCATACAGCAGCTATGAAATGCTCTCAGGACACATTTGGAGATGTGCCTGTAAAGCTAGAAATCTTGCTGAAGATCAAGAAACCAAATTGTATATTGCTACGGATGGTCGTAACCGGCTCAGACCATCCATTCCCCCTGGTTATTTTGGGAATGTTATTTTTACAACTACTCCCATGGCCGTTGCAGGGGACATTATATCAAAGCCTACATATTATGCTGCGAGTGTAATTCATGAAGCTTTGGGCCGGATGGATGATGAATACTTGCGTTCTGCTTTAGACTATTTGGAATTGCAGCCTGATTTGACTGCTCTAGTCAGAGGTTCTCATACTTTCAGGTGTCCAAATATTGGTATCACAAGCTGGTCTCGGTTGCCTATTCATGATGCAGACTTTGGGTGGGGAAGACCCATATTCATGGGACCTGGAGGTATTGCCTATGAAGGTCTTGCTTTCGTTTTGCCCAGTTCTGCCAATGATGGAAGTTTGTCAGTTGCATTGGGCTTGCAACCAGATCATATGGCTCGTTTTGCCAAAATGTTGTATGAAATCTAG___ATTTGACTTCTGTATAGGATTCAGGAACTAGAAATCGAAAAACCAGATTACATTATTTGTTTGGAACTGTAATTAGATTGAAGTACAACAATTTTGCCTTCTGTGAAAATTGAATCTGCTCAAAAATAAGATAAACAAACATGGAGAATTGTACTCGGGATCAAAATAAAAGATGACGCGAACTTGTTATTATCATCTTGATGTTGAAGTTGGAATCCGTTTAACCTGCAACTTCTCTATGATGTCAATGCATCTTGCCCCGGAATGATGCTTTAAGCTTTTCTTTGTACTCAGATTACAGAACTTTTGGCAAGTTCTTTTTCCCATACAGTATATATGCATGAACAATCTATAGAACCAGACTATATATTCTTGCTC

Fln Alignment:

Contig164___MLITVKSSQMVRPAAPTPQRDLWNSNVDLVVPRIHTASVYFYRPTGSPDFFSMNILRDALSKLLVPFYPMAGRLKRDPDGRIEINCNGEGVLLVEAITDSVIDDFGDFAPTMELKQLIPKVNYSEDISSYPLLVLQVTFFKCGGVSLGVGMQHHVADGYAGIHFINTWSDVARGLDITLPPFIDRTLLRARNPPTPKFQHIEYQQPPPLKDTSGIMNGEKNGFSVAIFKLTKEQLEILKGKARENGNNIAYSSYEMLSGHIWRCACKARNLAEDQETKLYIATDGRNRLRPSIPPGYFGNVIFTTTPMAVAGDIISKPTYYAASVIHEALGRMDDEYLRSALDYLELQPDLTALVRGSHTFRCPNIGITSWSRLPIHDADFGWGRPIFMGPGGIAYEGLAFVLPSSANDGSLSVALGLQPDHMARFAKM
A6Y879_____________MLITVKNSQMVRPAAPTPQRDLWNSNVDLVVPRIHTASVYFYRPTGSPDFFSMNILRDALSKLLVPFYPMAGRLKRDPDGRIEINCNGEGVLLVEAITDSVIDDFGDFAPTMELKQLIPKVNYSEDISSYPLLVLQVTFFKCGGVSLGVGMQHHVADGYAGIHFINTWSDVARGLDITLPPFIDRTLLRARNPPTPKFQHIEYQQPPPLKDTSGIMNGEKTDISVAIFKLTKEQLEILKGKARENGNNIAYSSYEMLSGHIWRCACKARNLAEDQETKLYIATDGRNRLRPSIPPGYFGNVIFTTTPMAVTGDIISKPTYYAASVIHEALGRMDDEYLRSALDYLELQPDLTALVRGAHTFRCPNIGITSWSRLPIHDADFGWGRPIFMGPGGIAYEGLAFVLPSSVNDGSLSVALGLQPDHMVRFAKM

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
1649	25		75		1.0
1746	72			27	1.0
1773	12		87		0.993502
1792			85	14	0.994477
1793	83	16			0.995451
1795		83		16	0.995451
1799		83		16	0.995451
1801		16		83	0.995451

SSRs (tot: 2)

Start position	End position	Sequence	Length
1933	1947	TGGTC TGGTC TGGTC	15
1944	1962	GGTCC GGTCC GGTCC GGTC	19

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain