SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene159

Length: 2350 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene159
ATGGTGATGATTCACTTTCGAAGTCCTTGGAGAAGCTGAAAAAATTCCAGAATGTGAAACCCCACCCTTCCTTCACTTCACGTCCTCACAATAAAAAGAGATTTAGCTTCAAAATCTCAGTATGCCTTGGCAAAAGATCGATGGGCGCATCGTGTGATGACTTCGACGATGGAAAAATATAAGACGAGAATCACTGATTGCGGCTTTGAGAACCTTTCACTGGCCGTGATTCAGGAAATCCTCCTTAAGCTAGACTTGGAATCTCTGTGTGCTGCAGCGTCAACATGTACGTCGTGACCTTGGCTGTCACTCAAGTTTTAAGATCATTTGATACCATCGACCTCTCCGAAGCTATTCCAGATAAGAAGGTCTTGAGTTATCTCCTTTCCAATAATGATTCAGTTAGGAATCTTACCATAAATTGCAGCCGACTGGATGACTCAGCAACATACATTTTTGCTCTTTCGCACCTCAAAGAGCTGGTTCTGCAGAAATGTTCCAATTTTTCTCACAGGCTTTTTGTTGCACTGGGGCAACATTGCCCCAATCTGAGATTGTTGTCCGTCGAGTTGGCATACTACCGTAGGCATGGGGCCCTTCAAAACTACAAATCAAGTCTCAAAAAACTTTTCGATGGTTGCCACTCTTTGGAATCCTTGTGCATCAAGTTTGAAGGGGCACAATTTGATCCTTGCATTTTTGAAGCGACAGCATCTTTCATGCCCATGACAGTAAAATACCTGCATCTGCAACAAGTAACAGAAAAAGATACAAGACAATCATTACACGCTTGGGGTATTCAAAGGATAGCTGCTGTCCCTGGACAAAATGGGATTCCCTTCTCAATGGGAGTTTCCTTCCAAAGTATACAGTCCTTAACACTTGTTGTAGATCATATCACAGATGACCTTCTGAGTTCTATCACACAAAATCTCATTCTCCTGGTTAATTTAGATCTGAGGGATGCACCTCTAGGAGAACCATTTCTACGACATGATCTGACCAATAGTGGTGTGCAGTCTTTGGGTTCTTGCAATTATTTATGTCACCTCTCCCTTTCCCGTAGCAGAGTAAATTATCCAGCATTTTTTAGACGAGTAAATGACATGGGCATGTTTCTCATGGCTGAAGGCTGTAAAAACATGGAGTCCATCAAGCTCGGAGGATTCTATAGAGTGACAGATGCTGGTTTTAGTGCACTCATACATGCTTGCAAAAATTTGAAGACTTTTGAACTCTATAACACATTTCATTTGTCTGATTTGGCTTTTCATGATCTTCTAGCTACTTCTCTTTCCCTTGTGTCTGTCAGACTGATCTCGTGCTGTCTTTTAACTAGTGAATCAGTGGAACACATTACTCTCTGTGCAGATCTAGAGGTGCTTGACCTCACAGGCTGTAAAAGTGTAGCAGATGATGGTATGCGAGCTATTTCAAAGCTTAAGAAACTGAATACTTTATCTCTTAGTGGAGCAGATATCACTGATACTGGAATGTCCATCTTAGGAAGTGGAAATGTTCCCTTGGTATCCTTATCTCTGCGTGGTTGTAAAAGAGTAACTGATCATGGAGTAGCACTTTTGTTGGGAGGTTCCACTAAGAAGACATTGAGAAAACTTGATTTGGGTTACATGCCTGCTATATCAGACAAGACAATAATTTTTTTGACAAAAGTGGGACTGGAGATAGTTGAACTTTGCATTCGCAATTGTTTTGCTGTCACAGACATGTCTATTGCAGCTCTTGCAGGTAAAGATTGTGATGGGGTGGTCAGACGAACACTTAGACGACTGGATCTCTACAATTGTAAAGGCTTATCTTTGGTCTCTCTACAATGGTTGAAAAAACCTTACTTTCCACGGCTGCGCTGGCTAGGTCTGGCATCTACTGCTCTGTCGGGTACGAGAGAACTGGATAAAATTACAGAGGAACGTCCATATTTAAACATATGCTGCACTGGTTGTGAAGTGGGCTGTCGTGATCTGTGGCAATCTCATGAGAATCTCTTTGTGTGAGTGAGCTATTTATCAATATTATTGCTTCATGACAGTAGTTGAAAAAACCATACTTTACATGCTTGTGCTGGCTAGGTATGCAATCTATTGCCCTTTTTAGTTTGAGAGGACTGTTTATATTTACAGCAGAGTGCCTTTCTTTAAGCATATGCAGGGCAGACCGTGAAGTGGGCTGTCATGATAGGCGGCATTTTCATGAGGGTGGATATGTAATGACTGATAGCTATGGATTATTTCTTTTGCAAGGACAATTAAATGGTGTAAATATGTGTAAAGGGAAATGTTTGCCATGCCAATTTCTCAATTTATGCTGGAATAATTATGAGCTTGGTAGG

Ace file of the UniGene sp_v2.0_unigene159 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	F-box protein At-B [Arabidopsis thaliana] sp\|Q9ZWC6.1\|ATB_ARATH RecName: Full=F-box protein At-B gb\|AAF79512.1\|AC002328_20 F20N2.2 [Arabidopsis thaliana] gb\|AAM26672.1\| At1g55590/F20N2_18 [Arabidopsis thaliana] gb\|AAN46816.1\| At1g55590/F20N2_18 [Arabidops	-	-	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	42%
Blast2go	f-box protein at-b-like	-	-	59%

Source	InterPros	Term	Type	Identity
Sma3	Reverse transcriptase	IPR000477	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3		IPR015706	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NV99

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 33 aas, putative chimeric sequence,

Fln protein: MYVVTLAVTQVLRSFDTIDLSEAIPDKKVLSYLLSNNDSVRNLTINCSRLDDSATYIFALSHLKELVLQKCSNFSHRLFVALGQHCPNLRLLSVELAYYRRHGALQNYKSSLKKLFDGCHSLESLCIKFEGAQFDPCIFEATASFMPMTVKYLHLQQVTEKDTRQSLHAWGIQRIAAVPGQNGIPFSMGVSFQSIQSLTLVVDHITDDLLSSITQNLILLVNLDLRDAPLGEPFLRHDLTNSGVQSLGSCNYLCHLSLSRSRVNYPAFFRRVNDMGMFLMAEGCKNMESIKLGGFYRVTDAGFSALIHACKNLKTFELYNTFHLSDLAFHDLLATSLSLVSVRLISCCLLTSESVEHITLCADLEVLDLTGCKSVADDGMRAISKLKKLNTLSLSGADITDTGMSILGSGNVPLVSLSLRGCKRVTDHGVALLLGGSTKKTLRKLDLGYMPAISDKTIIFLTKVGLEIVELCIRNCFAVTDMSIAALAGKDCDGVVRRTLRRLDLYNCKGLSLVSLQWLKKPYFPRLRWLGLASTALSGTRELDKITEERPYLNICCTGCEVGCRDLWQSHENLFV*

Protein Length: 578

Fln nts: ATGGTGATGATTCACTTTCGAAGTCCTTGGAGAAGCTGAAAAAATTCCAGAATGTGAAACCCCACCCTTCCTTCACTTCACGTCCTCACAATAAAAAGAGATTTAGCTTCAAAATCTCAGTATGCCTTGGCAAAAGATCGATGGGCGCATCGTGTGATGACTTCGACGATGGAAAAATATAAGACGAGAATCACTGATTGCGGCTTTGAGAACCTTTCACTGGCCGTGATTCAGGAAATCCTCCTTAAGCTAGACTTGGAATCTCTGTGTGCTGCAGCGTCAAC_-_ATGTACGTCGTGACCTTGGCTGTCACTCAAGTTTTAAGATCATTTGATACCATCGACCTCTCCGAAGCTATTCCAGATAAGAAGGTCTTGAGTTATCTCCTTTCCAATAATGATTCAGTTAGGAATCTTACCATAAATTGCAGCCGACTGGATGACTCAGCAACATACATTTTTGCTCTTTCGCACCTCAAAGAGCTGGTTCTGCAGAAATGTTCCAATTTTTCTCACAGGCTTTTTGTTGCACTGGGGCAACATTGCCCCAATCTGAGATTGTTGTCCGTCGAGTTGGCATACTACCGTAGGCATGGGGCCCTTCAAAACTACAAATCAAGTCTCAAAAAACTTTTCGATGGTTGCCACTCTTTGGAATCCTTGTGCATCAAGTTTGAAGGGGCACAATTTGATCCTTGCATTTTTGAAGCGACAGCATCTTTCATGCCCATGACAGTAAAATACCTGCATCTGCAACAAGTAACAGAAAAAGATACAAGACAATCATTACACGCTTGGGGTATTCAAAGGATAGCTGCTGTCCCTGGACAAAATGGGATTCCCTTCTCAATGGGAGTTTCCTTCCAAAGTATACAGTCCTTAACACTTGTTGTAGATCATATCACAGATGACCTTCTGAGTTCTATCACACAAAATCTCATTCTCCTGGTTAATTTAGATCTGAGGGATGCACCTCTAGGAGAACCATTTCTACGACATGATCTGACCAATAGTGGTGTGCAGTCTTTGGGTTCTTGCAATTATTTATGTCACCTCTCCCTTTCCCGTAGCAGAGTAAATTATCCAGCATTTTTTAGACGAGTAAATGACATGGGCATGTTTCTCATGGCTGAAGGCTGTAAAAACATGGAGTCCATCAAGCTCGGAGGATTCTATAGAGTGACAGATGCTGGTTTTAGTGCACTCATACATGCTTGCAAAAATTTGAAGACTTTTGAACTCTATAACACATTTCATTTGTCTGATTTGGCTTTTCATGATCTTCTAGCTACTTCTCTTTCCCTTGTGTCTGTCAGACTGATCTCGTGCTGTCTTTTAACTAGTGAATCAGTGGAACACATTACTCTCTGTGCAGATCTAGAGGTGCTTGACCTCACAGGCTGTAAAAGTGTAGCAGATGATGGTATGCGAGCTATTTCAAAGCTTAAGAAACTGAATACTTTATCTCTTAGTGGAGCAGATATCACTGATACTGGAATGTCCATCTTAGGAAGTGGAAATGTTCCCTTGGTATCCTTATCTCTGCGTGGTTGTAAAAGAGTAACTGATCATGGAGTAGCACTTTTGTTGGGAGGTTCCACTAAGAAGACATTGAGAAAACTTGATTTGGGTTACATGCCTGCTATATCAGACAAGACAATAATTTTTTTGACAAAAGTGGGACTGGAGATAGTTGAACTTTGCATTCGCAATTGTTTTGCTGTCACAGACATGTCTATTGCAGCTCTTGCAGGTAAAGATTGTGATGGGGTGGTCAGACGAACACTTAGACGACTGGATCTCTACAATTGTAAAGGCTTATCTTTGGTCTCTCTACAATGGTTGAAAAAACCTTACTTTCCACGGCTGCGCTGGCTAGGTCTGGCATCTACTGCTCTGTCGGGTACGAGAGAACTGGATAAAATTACAGAGGAACGTCCATATTTAAACATATGCTGCACTGGTTGTGAAGTGGGCTGTCGTGATCTGTGGCAATCTCATGAGAATCTCTTTGTGTGA___GTGAGCTATTTATCAATATTATTGCTTCATGACAGTAGTTGAAAAAACCATACTTTACATGCTTGTGCTGGCTAGGTATGCAATCTATTGCCCTTTTTAGTTTGAGAGGACTGTTTATATTTACAGCAGAGTGCCTTTCTTTAAGCATATGCAGGGCAGACCGTGAAGTGGGCTGTCATGATAGGCGGCATTTTCATGAGGGTGGATATGTAATGACTGATAGCTATGGATTATTTCTTTTGCAAGGACAATTAAATGGTGTAAATATGTGTAAAGGGAAATGTTTGCCATGCCAATTTCTCAATTTATGCTGGAATAATTATGAGCTTGGTAGG

Fln Alignment:

Contig159___VLSYLLSNNDSVRNLTINCSRLDDSATYIFALSHLKELVLQKCSNFSHRLFVALGQHCPNLRLLSVELAYY-----RRHGALQNYKSSLKKLFDGCHSLESLCIKFEGAQFDPCIFEATASFMPMTVKYLHLQQVTEKDTRQSLHAWGIQRIAAVPGQNGIPFS--MGVSFQSIQSLTLVVDHITDDLLSSITQNLILLVNLDLRDAPLGEPFLRHDLTNSGVQSLGSCNYLCHLSLSRSRVNYPAFFRRVNDMGMFLMAEGCKNMESIKLGGFYRVTDAGFSALIHACKNLKTFELYNTFHLSDLAFHDLLATSLSLVSVRLISCCLLTSESVEHITLCADLEVLDLTGCKSVADDGMRAIXXXXXXXXXXXSGADITDTGMSILGSGNVPLVSLSLRGCKRVTDHGVALLLGGSTKKTLRKLDLGYMPAISDKTIIFLTKVGLEIVELCIRNCFAVTDMSIAALAGKDCDG-VVRRTLRRLDLYNCKGLSLVSLQWLKKPYFPRLRWLGLASTALSGTRELDKITEERPYLNICCTGCEV
A9NV99_____________MLHHLLPPNLALKSLKVDCAQLGDSSIGHLANPQLEELCLRCCDNFTADLLFEIGRNCKNLRSLSLELGWLDEPEDRPRTFIHN--AGLEQLLRGCSQLESLCLTFDGSSFDNSKFAAIWRLVAPTLKVLELGYILATDAKEIFNSKIFSPGGNIFGHSTLQMQEQKSTAFPNLQKLCLVLDWISDSVVGVISKNLPFLIELDLRDEPIEEPRAAVDLTNWGIQQISSCSKLRHLSLVRSQEDFAISFRRVNDLGILLMAENCSNLESIRLGGFCRITDASFRAILHRCSNLQKLELLRMTQLTDLVFHDISATPLSLTDVSLISCSLITDFSIIHLAHCKDIQVLDLKGCRRVGDDGLKVVSSLGKLKLLHLNSSDISDVGLSYLGSGNAPLVSLSLRSCQRLTDKGISALVAGSLVQTLQNLDLSNIPNLTDNAILVLVKSGMQIVELRLRECPLIGDTSVIALASMHFQGRGYGSTLRLLDIYNSGGITKLAISWFKKPYFSRLRWLGIGSNV---NGYVDVLGRDRPLLRILWQGNEL

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
750	33			66	0.997452
751	33	66			0.997452
753			33	66	0.997452
754	66			33	0.997452
783		75		25	0.996473
944	88	11			0.923564
1040		71		28	1.0
1046			83	16	0.994512
1047	83			16	0.994512
1103	75	25			0.996473
1156	80	20			0.995493
1158		20		80	0.995493
1160	80			20	0.995493
1161	80		20		0.995493
1162	80			20	0.995493
1473		33		66	0.997452
1474		33		66	0.997452
1475	66	33			0.997452
1477			66	33	0.997452
1566	37		62		1.0
1591		12		87	0.992554
1592	12		87		0.992554
2044	66			33	0.997452
2052	66		33		0.997452
2055			33	66	0.997452
2056	66			33	0.997452
2059		33	66		0.997452
2063		33		66	0.997452
2064		66	33		0.997452
2232	33			66	0.997452
2347	33			66	0.997452
2349	33	33	33		0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain