SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene106

Length: 2384 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene106
CGTCGTGACCTTGGCTGTCACTCAATAAATTCTCTTTCCCCTTGGTGGATTAACACAGCAGAACAGCAGGAACCATATTTGTTCTGTTCATACTCTGGAGACAGCATGCAGCAGGGCAAGTTTCCTCTGTTCAAATGGCCCTGGGAAAGCCTCAACAACTACAAGTATTATCTATATGTGCCCTTAGCACTAATGGCTGCTCATGAAATATGGTTTCGTGGAAAACATAATGATTACTGGTTCGTGTACGTTCTCGTAATATCTTTCTTAAGGTGTGTGATATACCAAACTTGGCACCTTTATTCTCGAGCGGATTTTCTTTCAAAGGGACAACAAATTCACCCTTTCTCTACCGATTTCGATCAGATTGACAGAGAATTACACTGGGATAATTATATAATATTTCAAGCTTGGGTGGCAACTTTGGGCTATTGTTTTTTCCTGGGTTAAGCAACCTTCCAATCTGGAATTACAGAGGGATGATCTCAGTCTTGCTGCTGCACATGGGCCCAACTGAGCTACTTTACTACTGCATTCATAGATCTTTTCACAGAGGTTCCTTATTTGAAAATTACCATTCTCTTCACCATGCATCCACCAGGCCTGAACCTTCAACAGCTGGTACCACCTCTTTCTTGGAGCAGTTGGTGATGGTTTCATTGATGACGATTCCCATATTTGGGGTGGTTTTGATGGGCGAGGGATCTATTGGCATGTTCTTTGTATACTTCCTGGGGTTTGATTTCATGAAGTTTATGATTCACTCAAATGCTGAGGTAGTTCCCCTGTGGCTTTTTCGTACGTTTCCATCGCTCAAGTATCTTCTAGTGACGCCCTCGTATCATCATGTGCATCACACCAAACTGAACTCAAATTATTGCCTCTTTATGCCTCTATACGATTATTTGGGAGGGACATTGAACCAAGATGCCTTTAATCTCCATTTTAACATGCGTTGCCAAGGCCAGAAGAGGAGAGCTCCAGATCTTGTGTTCTTGGGTCATGGTGTAGACTTCACAGGCCTTGTTCATGTTCCATATTTTGGACGATCTTTTGCCGCACAGCCATGTTCTTCTCAATGGTATGTGTGGCCTATTATGCCACTCATCTGGCTTGGTTTCTTTGGATGGCAGTTTACAAACACACATATAATCTCGAGGTACACTATTGGAGAGTTTAATCAGGAAACATGGGCTATACCGCGACTTGGTTTTCAGTTTTTCCTGCCATTTGGAAAGGATAAAATCAACGAACTTATTGAGGAGGCCATTCTTGAAGCAGATAGAATGGGAGTGAAAGTAATTTCTCTCGCAGGTCTAGTTAAGAATGAGGTCTTGAATGGAGGGGGACAACTATTTGTGAACAAGCATCCTAATCTTCACATTCGGGTGGCCCATGGAAATACTTTAACGACAGCTGTGCTTTTACATGAACTTCCACACGATGTAGATGCAGTATTCCTCACTGGCTCAACGTCAAAAATTGGCAAGGCTATTGCTCTCTACCTCTGCAAGAAACGAGTTAGAGTCTTGATGTTTACAGCATCCTTTGAGCGATTTAAAGCAGTGAGGAATCAAGCCCCTGCTGAATATCAACAATACCTCGAACATGTTTCTAAATATGAAGCAGGCAGGTTTTGCAAGACATGGATCATAGGCAAATGGGCAGGCTACGAAGATCAAATGTGGGCTCCTTCAGGAACCCACTTTTACCAGTTTACTATACCAATGATTATTGAATTCAGGAAAGATTGCACGTATGGAGGTTCATCAGCACTTAAATTACCAGACACCGCCAGAGGCGTGAATGGCTGTGAGCATAAGATGCCGAGAAGAGTAATACACGCATGCCATGCAGGAGGGTTGATACATGCTCTTGAAGGCTATGAGCATCATGAAGTTGGGGCAATCGATGTAGATCGCCTTGATATTGTATGGGAGGCAGCAATGAAACATGGACTCAGGCCTGTTTCACAAAGCAAGATTCAGTAATTGATTTCTATTTCTGTTAGATTGGAATGTTGGTTTATTTTAGTATCGGTTCTCAATCGCTGGCCCAGTCATTCATCTATCTAATAAAGGTATTTATGTGCACAATTGCACAACTAGACTTTTGGAAGGCTGCTTATTTTCTTGGTACCGATCGTCCACCTATTCTCATATACTGATGAGAAATGGACCTGTTATGTCGATCTACCAGCATATTCATATTAAGTTTGACTCATAACATGCAACTAGTGAGGAATAATGAGCCTGATGAATTTTAATAAAGGCTACAAAAGTTGCCTCATACTATCTGAAAGTTTGGTTCTAGTGATATGCTACTTTAAAATAAAATCGCAGTTCACATAGACCTACATCAAATCATCATAGCTTTTCATAAAG

Ace file of the UniGene sp_v2.0_unigene106 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Wax2_C multi-domain protein	-	-	44%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	40%
Blast2go	protein wax2-like	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	wax biosynthetic process	GO:0010025	Biological Process	70%
Blast2go	pollen sperm cell differentiation	GO:0048235	Biological Process	70%
Blast2go	cuticle hydrocarbon biosynthetic process	GO:0006723	Biological Process	70%
Blast2go	binding	GO:0005488	Molecular Function	70%
Blast2go	catalytic activity	GO:0003824	Molecular Function	70%
Blast2go	plasma membrane	GO:0005886	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Blue (type 1) copper domain	IPR000923	-	-
Sma3	WW/Rsp5/WWP	IPR001202	-	-
Sma3	DNA methylase, N-6 adenine-specific, conserved site	IPR002052	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Fatty acid hydroxylase	IPR006694	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A835

Fln msg: Separated hits, possible frame ERROR between 438 and 465, No stop codon before M and low homology subject,

Fln protein: MQQGKFPLFKWPWESLNNYKYYLYVPLALMAAHEIWFRGKHNDYWFVYVLVISFLRCVIYQTWHLYSRADFLSKGQQIHPFSTDFDQIDRELHWDNYIIFQAWVATLGYCxxxxxxxxxxWNYRGMISVLLLHMGPTELLYYCIHRSFHRGSLFENYHSLHHASTRPEPSTAGTTSFLEQLVMVSLMTIPIFGVVLMGEGSIGMFFVYFLGFDFMKFMIHSNAEVVPLWLFRTFPSLKYLLVTPSYHHVHHTKLNSNYCLFMPLYDYLGGTLNQDAFNLHFNMRCQGQKRRAPDLVFLGHGVDFTGLVHVPYFGRSFAAQPCSSQWYVWPIMPLIWLGFFGWQFTNTHIISRYTIGEFNQETWAIPRLGFQFFLPFGKDKINELIEEAILEADRMGVKVISLAGLVKNEVLNGGGQLFVNKHPNLHIRVAHGNTLTTAVLLHELPHDVDAVFLTGSTSKIGKAIALYLCKKRVRVLMFTASFERFKAVRNQAPAEYQQYLEHVSKYEAGRFCKTWIIGKWAGYEDQMWAPSGTHFYQFTIPMIIEFRKDCTYGGSSALKLPDTARGVNGCEHKMPRRVIHACHAGGLIHALEGYEHHEVGAIDVDRLDIVWEAAMKHGLRPVSQSKIQ*

Protein Length: 630

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATAAATTCTCTTTCCCCTTGGTGGATTAACACAGCAGAACAGCAGGAACCATATTTGTTCTGTTCATACTCTGGAGACAGC_-_ATGCAGCAGGGCAAGTTTCCTCTGTTCAAATGGCCCTGGGAAAGCCTCAACAACTACAAGTATTATCTATATGTGCCCTTAGCACTAATGGCTGCTCATGAAATATGGTTTCGTGGAAAACATAATGATTACTGGTTCGTGTACGTTCTCGTAATATCTTTCTTAAGGTGTGTGATATACCAAACTTGGCACCTTTATTCTCGAGCGGATTTTCTTTCAAAGGGACAACAAATTCACCCTTTCTCTACCGATTTCGATCAGATTGACAGAGAATTACACTGGGATAATTATATAATATTTCAAGCTTGGGTGGCAACTTTGGGCTATTGTTTxxxxxxxxxxxxxxxxxxxxxxxxxxxCTGGAATTACAGAGGGATGATCTCAGTCTTGCTGCTGCACATGGGCCCAACTGAGCTACTTTACTACTGCATTCATAGATCTTTTCACAGAGGTTCCTTATTTGAAAATTACCATTCTCTTCACCATGCATCCACCAGGCCTGAACCTTCAACAGCTGGTACCACCTCTTTCTTGGAGCAGTTGGTGATGGTTTCATTGATGACGATTCCCATATTTGGGGTGGTTTTGATGGGCGAGGGATCTATTGGCATGTTCTTTGTATACTTCCTGGGGTTTGATTTCATGAAGTTTATGATTCACTCAAATGCTGAGGTAGTTCCCCTGTGGCTTTTTCGTACGTTTCCATCGCTCAAGTATCTTCTAGTGACGCCCTCGTATCATCATGTGCATCACACCAAACTGAACTCAAATTATTGCCTCTTTATGCCTCTATACGATTATTTGGGAGGGACATTGAACCAAGATGCCTTTAATCTCCATTTTAACATGCGTTGCCAAGGCCAGAAGAGGAGAGCTCCAGATCTTGTGTTCTTGGGTCATGGTGTAGACTTCACAGGCCTTGTTCATGTTCCATATTTTGGACGATCTTTTGCCGCACAGCCATGTTCTTCTCAATGGTATGTGTGGCCTATTATGCCACTCATCTGGCTTGGTTTCTTTGGATGGCAGTTTACAAACACACATATAATCTCGAGGTACACTATTGGAGAGTTTAATCAGGAAACATGGGCTATACCGCGACTTGGTTTTCAGTTTTTCCTGCCATTTGGAAAGGATAAAATCAACGAACTTATTGAGGAGGCCATTCTTGAAGCAGATAGAATGGGAGTGAAAGTAATTTCTCTCGCAGGTCTAGTTAAGAATGAGGTCTTGAATGGAGGGGGACAACTATTTGTGAACAAGCATCCTAATCTTCACATTCGGGTGGCCCATGGAAATACTTTAACGACAGCTGTGCTTTTACATGAACTTCCACACGATGTAGATGCAGTATTCCTCACTGGCTCAACGTCAAAAATTGGCAAGGCTATTGCTCTCTACCTCTGCAAGAAACGAGTTAGAGTCTTGATGTTTACAGCATCCTTTGAGCGATTTAAAGCAGTGAGGAATCAAGCCCCTGCTGAATATCAACAATACCTCGAACATGTTTCTAAATATGAAGCAGGCAGGTTTTGCAAGACATGGATCATAGGCAAATGGGCAGGCTACGAAGATCAAATGTGGGCTCCTTCAGGAACCCACTTTTACCAGTTTACTATACCAATGATTATTGAATTCAGGAAAGATTGCACGTATGGAGGTTCATCAGCACTTAAATTACCAGACACCGCCAGAGGCGTGAATGGCTGTGAGCATAAGATGCCGAGAAGAGTAATACACGCATGCCATGCAGGAGGGTTGATACATGCTCTTGAAGGCTATGAGCATCATGAAGTTGGGGCAATCGATGTAGATCGCCTTGATATTGTATGGGAGGCAGCAATGAAACATGGACTCAGGCCTGTTTCACAAAGCAAGATTCAGTAATTGATTTCTATTTCTGTTAGATTGGAATGTTGGTTTATTTTAGTATCGGTTCTCAATCGCTGGCCCAGTCATTCATCTATCTAATAAAGGTATTTATGTGCACAATTGCACAACTAGACTTTTGGAAGGCTGCTTATTTTCTTGGTACCGATCGTCCACCTATTCTCATATACTGATGAGAAATGGACCTGTTATGTCGATCTACCAGCATATTCATATTAAGTTTGACTCATAACATGCAACTAGTGAGGAATAATGAGCCTGATGAATTTTAATAAAGGCTACAAAAGTTGCCTCATACTATCTGAAAGTTTGGTTCTAGTGATATGCTACTTTAAAATAAAATCGCAGTTCACATAGACCTACATCAAATCATCATAGCTTTTCATAAAG

Fln Alignment:

Contig106___LFKWPWESLNNYKYYLYVPLALMAAHEIWFRGKHNDYWFVYVLVISFLRCVIYQTWHLYSRADFLSKGQQIHPFSTDFDQIDRELHWDNYIIFQAWVATLGYCFxxxxxxxxxWNYRGMISVLLLHMGPTELLYYCIHRSFHRGSLFENYHSLHHASTRPEPSTAGTTSFLEQLVMVSLMTIPIFGVVLMGEGSIGMFFVYFLGFDFMKFMIHSNAEVVPLWLFRTFPSLKYLLVTPSYHHVHHTKLNSNYCLFMPLYDYLGGTLNQDAFNLHFNMRCQGQKRRAPDLVFLGHGVDFTGLVHVPYFGRSFAAQPCSSQWYVWPIMPLIW-LGFFGWQFTNTHIISRYTIGEFNQETWAIPRLGFQFFLPFGKDKINXXXXXXXXXADRMGVKVISLAGLVKNEVLNGGGQLFVNKHPNLHIRVAHGNTLTTAVLLHELPHDVDAVFLTGSTSKIGKAIALYLCKKRVRVLMFTASFERFKAVRNQAPAEYQQYLEHVSKYEAGRFCKTWIIGKWAGYEDQMWAPSGTHFYQFTIPMIIEFRKDCTYGGSSALKLPDTARGVNGCEHKMPRRVIHACHAGGLIHALEGYEHHEVGAIDVDRLDIVWEAAMKHGLRPV
D5A835_____________LLDWPWAYLESLKYVLYGPLIAKAVHTNLYGGKEADNWCFHILLLTSLRYVTYQLWATFSNMYCLSHRYKICKKGAEFDQMDREWDWDNFLLLQAFMATAAHHFxxxxxxxxxWNAGGLICLAILRMGPAEVLYYWAHRAFHKDFLFQRYHSLHHAAIVLQPQTAGTATFLEHIGLTIIMAVPMVGASWMGGASMGMIYIYCLLFDFLRYMGHSNVEIVPETIFRCLPPLKYLIYTPLYHTLHHTEMDTNFCPFMPLYDYLGHTINSKSWDLHRSMSA-GQVEDVPDYVFLAHIVDVLSSLHVRFLLRGFCSTPFATWWFLLPLWPVVIPVALAMWVWAKTFVNTGHRLKGRLHQTWIVPRFGFQYFIPFAQAGINNLIQDAILSADKMGVKVISLAALNKNEALNGGGTLFVNRLPDLRVRVVHGNTLTAAVILNELNPDVKEVFLTGATSKLGRVIALYLCRKGIRVMMLTYSKERFKSIQSEAPPEFQNFLVQVTKYEAAQNCKTWIVGKWIAYKEQTWAPVGCHLHQFVVPPIFELRKDCTYGKLAGMQLPDAVEGLSTCEYTMPRRCVHACHAGGILHSLEGWEHHEVGAIDVNKIDMVWEAALKHGFKPM

SNPs (tot: 40)

Position	A %	C %	G %	T %	Probability
1213		71	28		1.0
1307	10			90	0.90298
1310	90			10	0.90298
1337		10		90	0.90298
1338	10	90			0.90298
1339			90	10	0.90298
1447		12	87		0.999964
1451	87		12		0.999964
1454		11	88		0.999952
1458	11			88	0.999952
1459		88	11		0.999952
1460	11			88	0.999952
1463		12	87		0.999964
1466		87		12	0.999964
1470		12	87		0.999964
1474	12	81		6	0.999943
1476		86		13	0.999973
1477		80		13	0.999961
1478		80		13	0.999961
1602	12		87		0.992554
1603		12	87		0.992554
1605	87			12	0.992554
1606		87		12	0.992554
1700	16	83			0.994512
1701	83			16	0.994512
1702		83		16	0.994512
1712	20		80		0.995493
1713		20	80		0.995493
1716	83			16	0.994512
1777	16		83		0.994512
1845		80	20		0.995493
1888	25		75		0.996473
1891			75	25	0.996473
1892		75		25	0.996473
1973		66		33	0.997452
1974		66		33	0.997452
2076		57		42	1.0
2283	16		83		0.994512
2285	16			83	0.994512
2286			83	16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain