SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene51

Length: 2473 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene51
CGTCGTGACCTTGGCTGTCACTCTATTCCAATTTGACCAAATGTAGGTTGGTTCAACCTTATAACAATGGCATGTCGGGACGAGGTAGTGGCTGTGCGGAAGCATGGAGGGGAGGCCGTCTACCTCTTATTCGTTCTCAGGTGACCCTGCAGCCCCCAGTGGAAGTCGGCAGACGCCGAAGGTCATTCTTCTGCAACATCCTCCTCCATCTTCTGCATCATCTTTAATTCGTCCCAGTCCTAGTCATTTTGCGAAATGGCTTTCCAGGATACGAAGAAAGTCTGCCATGGAATGGCTGGAGCTGTTGCTGCCCTGCACCCGCTGGATTCGAACCTACAAATGGAGAGAATACCTGCAGGCCGACATTATGGCCGGGATCACAGTCGGCACAATGCTCGTTCCTCAGGCAATGTCATATGCAAAGTTAGCTGGACTTCACCCAATTTATGGACTTTATTCTGGTTTTGTTCCTGTATTTGCATATGCAATCTTTGGCTCATCGAGGCAACTTGCCATTGGACCCGTAGCGTTGGTTTCACTTTTGGTTTCTAACACCTTATCCAGTATTGTGGATTCAACTGATGACTTATACACACAGCTTGCAATACTGTTGGCTCTTCTAGTTGGCATCTTGGAGTGTTTAATGGGAATTCTCAGGCTTGGATGGCTTATTCGTTTTATAAGCCATTCTGTCATTTCAGGTTTCACCACATCTTCAGCTATAGTTATTGCCCTGTCACAGGCAAAATATTTCCTTGGATATAGTATCACTCGCAGCAGCAAAATAATACCACTTGTTAAAAGCATAATTGCTGGTGCTGACAAGTTTTCATGGCCTCCATTTGTTATGGGTTCTATAATGCTGGCCATTCTTCTGACAATGAAGCAGCTGGGGAAAAAACATAAAAAGCTGAGATTCTTGCGAGTGGCGGGGCCTCTTACAGCAGTAATTTTGGGAACTGTTTTTGTCAAGATTCTCCATCCTCAATCTATTAGTGTGGTGGGTGGAATTCCTGAAGGCCTTCCAAGTTTTTCAGTTCCAACATGCTTTGATTATGTAAAGCGTTTAATACCAACAGCCCTTCTTATAACGGGTGTGGCTATCCTCGAATCAGTAGGAATTGCTAAAGCACTTGCAGCAAAGAATGGCTATGAACTAGATTCAAATCAAGAATTATTTGGGCTTGGAGTGGCCAATATTTGCGGTTCATTCTTTTCTTCATATCCTACCACGGGATCATTTTCAAGATCGGCTGTTAATAATGAAAGTGGTGCCAAGACCGGTTTATCTGGATTCATTATGGGAGTGATAATTCTCTGCGCTCTTCAATTTTTGACACCTCTTTTTACAGATATACCTCAATGCACTTTGGCTGCAATTGTGGTATCAGCTGTCATGGGTCTGATTGATTATGAAGAGGCTATATTTCTTTGGCATGTTGATAAAAGAGATTTCCTTTTATGGGTTACAACAAGTATTACCACGCTATTCCTTGGCATTGAGATCGGAGTACTTGTTGGAGTTGGTGCATCTCTTGCATTTGTGATACATGAATCAGCAAATCCACATATCGCTGTACTAGGTCGTCTTCCTGGCACTACAGTATACAGAAACATCAAGCAATACCCAGAGGCCTATACATACAAAGGGATTGTTGTTGTCCGCATTGATGCACCTATTTATTTTGCAAATATAAGCTATATCAAAGAGAGGTTACAAAAATATGAAGTTGGCTTTGATGGGACTACAAATTCTGGAATTGAAGTTAATAAAATGTCCTATGTTGTCATCGAGATGGCTCCGGTCACGTATATTGACTCCAGCGCTGCTCAGGCCTTGAAGGAGCTCTATCAAGAATACAAAGCACGCAACATTCAGATGGCCCTCTCGAACCCTAATCGAGAAGTTCTCTCAACACTTGCCATGGCTGGAGTCCTTGAACTTCTTGGCAAGCAGTGGTATTTTGTGAGAGTCCACGATGCTGTTCAAGTTTGCCTTTCCCACATGCAGGGAAGGTTTGAAACATCAAACACCAGCGGTGAAGAGCCCAAACAAGATATTGTAAGAAAGAAAGGAAATACTTCACTATCAAGCTTCTTCTTTGGGAAGGATGCTGAAGAATTAGACGCAGAAAGACAGCCTCTCATTATCGAAATACCTGAGGATTGAGAATATTTGCACAACACTACGACTTTGGTCCGCGATTCACTATGTATTCCTGGCTGTTATTCGTTGTATTCTTTTTATACACGCTTGTATTATATTGCCAGTAACACTGTTTCTCTGAACAGAAAGGCAGCACTGGTATTATTTTCAGGTGCAAAAAATGTTATTTCATTTTAATTGTGACATCAAAATATGTACCAATGTACCCATCAACATTAGAAACAAAATATCAATAACGTTTCAAGGCTTATTATGGACATTTACAACTTATTTTATATAGACCAAAATGCTCTCTTTGCCATTGCC

Ace file of the UniGene sp_v2.0_unigene51 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	sulfate transporter [Populus tremula x Populus alba]	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	sulfate transporter	-	-	85%

Source	GOs	Term	Type	Identity
Blast2go	sulfate transport	GO:0008272	Biological Process	85%
Blast2go	transmembrane transport	GO:0055085	Biological Process	85%
Blast2go	secondary active sulfate transmembrane transporter activity	GO:0008271	Molecular Function	85%
Blast2go	integral to membrane	GO:0016021	Cellular Component	85%

Source	InterPros	Term	Type	Identity
Sma3	Sulphate transporter/antisigma-factor antagonist STAS	IPR002645	-	-
Sma3	Sulphate anion transporter	IPR001902	-	-
Sma3	Sulphate transporter	IPR011547	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NX60

Fln msg:

Fln protein: MEGRPSTSYSFSGDPAAPSGSRQTPKVILLQHPPPSSASSLIRPSPSHFAKWLSRIRRKSAMEWLELLLPCTRWIRTYKWREYLQADIMAGITVGTMLVPQAMSYAKLAGLHPIYGLYSGFVPVFAYAIFGSSRQLAIGPVALVSLLVSNTLSSIVDSTDDLYTQLAILLALLVGILECLMGILRLGWLIRFISHSVISGFTTSSAIVIALSQAKYFLGYSITRSSKIIPLVKSIIAGADKFSWPPFVMGSIMLAILLTMKQLGKKHKKLRFLRVAGPLTAVILGTVFVKILHPQSISVVGGIPEGLPSFSVPTCFDYVKRLIPTALLITGVAILESVGIAKALAAKNGYELDSNQELFGLGVANICGSFFSSYPTTGSFSRSAVNNESGAKTGLSGFIMGVIILCALQFLTPLFTDIPQCTLAAIVVSAVMGLIDYEEAIFLWHVDKRDFLLWVTTSITTLFLGIEIGVLVGVGASLAFVIHESANPHIAVLGRLPGTTVYRNIKQYPEAYTYKGIVVVRIDAPIYFANISYIKERLQKYEVGFDGTTNSGIEVNKMSYVVIEMAPVTYIDSSAAQALKELYQEYKARNIQMALSNPNREVLSTLAMAGVLELLGKQWYFVRVHDAVQVCLSHMQGRFETSNTSGEEPKQDIVRKKGNTSLSSFFFGKDAEELDAERQPLIIEIPED*

Protein Length: 690

Fln nts: CGTCGTGACCTTGGCTGTCACTCTATTCCAATTTGACCAAATGTAGGTTGGTTCAACCTTATAACAATGGCATGTCGGGACGAGGTAGTGGCTGTGCGGAAGC_-_ATGGAGGGGAGGCCGTCTACCTCTTATTCGTTCTCAGGTGACCCTGCAGCCCCCAGTGGAAGTCGGCAGACGCCGAAGGTCATTCTTCTGCAACATCCTCCTCCATCTTCTGCATCATCTTTAATTCGTCCCAGTCCTAGTCATTTTGCGAAATGGCTTTCCAGGATACGAAGAAAGTCTGCCATGGAATGGCTGGAGCTGTTGCTGCCCTGCACCCGCTGGATTCGAACCTACAAATGGAGAGAATACCTGCAGGCCGACATTATGGCCGGGATCACAGTCGGCACAATGCTCGTTCCTCAGGCAATGTCATATGCAAAGTTAGCTGGACTTCACCCAATTTATGGACTTTATTCTGGTTTTGTTCCTGTATTTGCATATGCAATCTTTGGCTCATCGAGGCAACTTGCCATTGGACCCGTAGCGTTGGTTTCACTTTTGGTTTCTAACACCTTATCCAGTATTGTGGATTCAACTGATGACTTATACACACAGCTTGCAATACTGTTGGCTCTTCTAGTTGGCATCTTGGAGTGTTTAATGGGAATTCTCAGGCTTGGATGGCTTATTCGTTTTATAAGCCATTCTGTCATTTCAGGTTTCACCACATCTTCAGCTATAGTTATTGCCCTGTCACAGGCAAAATATTTCCTTGGATATAGTATCACTCGCAGCAGCAAAATAATACCACTTGTTAAAAGCATAATTGCTGGTGCTGACAAGTTTTCATGGCCTCCATTTGTTATGGGTTCTATAATGCTGGCCATTCTTCTGACAATGAAGCAGCTGGGGAAAAAACATAAAAAGCTGAGATTCTTGCGAGTGGCGGGGCCTCTTACAGCAGTAATTTTGGGAACTGTTTTTGTCAAGATTCTCCATCCTCAATCTATTAGTGTGGTGGGTGGAATTCCTGAAGGCCTTCCAAGTTTTTCAGTTCCAACATGCTTTGATTATGTAAAGCGTTTAATACCAACAGCCCTTCTTATAACGGGTGTGGCTATCCTCGAATCAGTAGGAATTGCTAAAGCACTTGCAGCAAAGAATGGCTATGAACTAGATTCAAATCAAGAATTATTTGGGCTTGGAGTGGCCAATATTTGCGGTTCATTCTTTTCTTCATATCCTACCACGGGATCATTTTCAAGATCGGCTGTTAATAATGAAAGTGGTGCCAAGACCGGTTTATCTGGATTCATTATGGGAGTGATAATTCTCTGCGCTCTTCAATTTTTGACACCTCTTTTTACAGATATACCTCAATGCACTTTGGCTGCAATTGTGGTATCAGCTGTCATGGGTCTGATTGATTATGAAGAGGCTATATTTCTTTGGCATGTTGATAAAAGAGATTTCCTTTTATGGGTTACAACAAGTATTACCACGCTATTCCTTGGCATTGAGATCGGAGTACTTGTTGGAGTTGGTGCATCTCTTGCATTTGTGATACATGAATCAGCAAATCCACATATCGCTGTACTAGGTCGTCTTCCTGGCACTACAGTATACAGAAACATCAAGCAATACCCAGAGGCCTATACATACAAAGGGATTGTTGTTGTCCGCATTGATGCACCTATTTATTTTGCAAATATAAGCTATATCAAAGAGAGGTTACAAAAATATGAAGTTGGCTTTGATGGGACTACAAATTCTGGAATTGAAGTTAATAAAATGTCCTATGTTGTCATCGAGATGGCTCCGGTCACGTATATTGACTCCAGCGCTGCTCAGGCCTTGAAGGAGCTCTATCAAGAATACAAAGCACGCAACATTCAGATGGCCCTCTCGAACCCTAATCGAGAAGTTCTCTCAACACTTGCCATGGCTGGAGTCCTTGAACTTCTTGGCAAGCAGTGGTATTTTGTGAGAGTCCACGATGCTGTTCAAGTTTGCCTTTCCCACATGCAGGGAAGGTTTGAAACATCAAACACCAGCGGTGAAGAGCCCAAACAAGATATTGTAAGAAAGAAAGGAAATACTTCACTATCAAGCTTCTTCTTTGGGAAGGATGCTGAAGAATTAGACGCAGAAAGACAGCCTCTCATTATCGAAATACCTGAGGATTGA___GAATATTTGCACAACACTACGACTTTGGTCCGCGATTCACTATGTATTCCTGGCTGTTATTCGTTGTATTCTTTTTATACACGCTTGTATTATATTGCCAGTAACACTGTTTCTCTGAACAGAAAGGCAGCACTGGTATTATTTTCAGGTGCAAAAAATGTTATTTCATTTTAATTGTGACATCAAAATATGTACCAATGTACCCATCAACATTAGAAACAAAATATCAATAACGTTTCAAGGCTTATTATGGACATTTACAACTTATTTTATATAGACCAAAATGCTCTCTTTGCCATTGCC

Fln Alignment:

Contig51___MEGRPSTSYSFSGDPAAPSGSRQTPKVILLQHXXXXXXXXXXXXXXSHFAKWLSRIRRKSAMEWLELLLPCTRWIRTYKWREYLQADIMAGITVGTMLVPQAMSYAKLAGLHPIYGLYSGFVPVFAYAIFGSSRQLAIGPXXXXXXXXXXXXXXXVDSTDDXXXXXXXXXXXXXXXXXXLMGILRLGWLIRFISHSVISGFTTSSAIVIALSQAKYFLGYSITRSSKIIPLVKSIIAGADKFSWPPFVMGSIMLAILLTMKQLGKKHKKLRFLRVAGPLTAVILGTVFVKILHPQSISVVGGIPEGLPSFSVPTCFDYVKRLIPTALLITGVAILESVGIAKALAAKNGYELDSNQELFGLGVANICGSFFSSYPTTGSFSRSAVNNESGAKTGLSGFIMGVIILCALQFLTPLFTDIPQCTLAAIVVSAVMGLIDYEEAIFLWHVDKRDFLLWVTTSITTLFXXXXXXXXXXXGASLAFVIHESANPHIAVLGRLPGTTVYRNIKQYPEAYTYKGIVVVRIDAPIYFANISYIKERLQKYEVGFDGTTNSGIEVNKMSYVVIEMAPVTYIDSSAAQALKELYQEYKARNIQMALSNPNREVLSTLAMAGVLELLGKQWYFVRVHDAVQVCLSHMQGRFETSNTSGEEPKQDIVRKKGNTSLSSFFFG-KDAEELDAERQPLIIEIPED
A9NX60____________MEWRPSSSYASSSSPATPGGSRQTPKVILLQHPPPSSTSSLIRPSPSHFAKWLSRIQRMPAMEWLELLLPCTRWIRTYKWREYLQADIMAGVTVGTMLVPQAMSYAKLAGLHPIYGLYSGFVPVFAYAIFGSSRQLAIGPVALVSLLVSNTLSSIVDSTDELYTELAILLALLVGILECVMGILRLGWLIRFISHSVISGFTTSSAIVIALSQAKYFLGYSITRTSKIIPLVKSIVAGADKFSWPPFVMGSIMLAILLTMKQLGKKRKKLRFLRVAGPLTAVILGTVYVKIFHPQSISVVGGIPEGLPSFSVPTCFDYVKRLIPTALLITGVAILESVGIAKALAAKNGYELDSNQELFGLGVANICGSFFSAYPTTGSFSRSAVNHESGAKTGLSGFIMGVIILCALQFLTPLFTDIPQCTLAAIVVSAVMGLIDYEEAIFLWRVDKRDFLLWVATSITTLFLGIEIGVLVGVGASLAFVIHESANPHIAVLGRLPGTTVYRNIKQYSEAYTYKGIVVVRIDAPIYFANISYIKERLQKYEVGFNGTTNSGIEGNKMFYVVIEMAPVTYIDSSAAQALKELYQEYKARNIQMALSNPNREVLSTLAMSGVLELVGKQWYFVRVHDAVQVCLSHMQGNLETLNTGGEEPKQDTVRKRGNTSLSNFFLGKKDAEELDTERQPLIIEIPED

SNPs (tot: 118)

Position	A %	C %	G %	T %	Probability
282	87			12	0.992554
381	11		88		0.923564
392	88		11		0.923564
506		11		88	0.923564
508	88	11			0.923564
511	88	11			0.923564
513	88			11	0.923564
581		11	88		0.923564
582		11	88		0.923564
583		11		88	0.923564
625		12		87	0.992554
649		12	87		0.992554
651		12	87		0.992554
652	12	87			0.992554
666		14	85		0.993533
700	87	12			0.992554
701		12		87	0.992554
702	12			87	0.992554
788	14	85			0.993533
789	83	16			0.994512
803		80		20	0.995493
884		14	85		0.993533
885	85		14		0.993533
1039		75		25	0.996473
1106	40		60		1.0
1153	20		80		0.995493
1154		80		20	0.995493
1156		80		20	0.995493
1158	16		83		0.994512
1159	83			16	0.994512
1171	14	85			0.993533
1172	14	85			0.993533
1173		85		14	0.993533
1174	85		14		0.993533
1240		85	14		0.993533
1241	14	85			0.993533
1243	14		85		0.993533
1244			14	85	0.993533
1245		14	85		0.993533
1246		14	85		0.993533
1247	12			87	0.992554
1254		12		87	0.992554
1257	87	12			0.992554
1259	85	14			0.993533
1260	85	14			0.993533
1264	85		14		0.993533
1267		14	85		0.993533
1269	85	14			0.993533
1270		85	14		0.993533
1279		16		83	0.994512
1280		16		83	0.994512
1281		16	83		0.994512
1282	16		83		0.994512
1283		83		16	0.994512
1532	16	83			0.994512
1536	83		16		0.994512
1538		16	83		0.994512
1541		16	83		0.994512
1543	16	83			0.994512
1544	16	83			0.994512
1545		50	16	16	0.988986
1547		83		16	0.994512
1551			16	83	0.994512
1563	20	80			0.995493
1599		85		14	0.993533
1737	11			88	0.923564
1738		11	88		0.923564
1741	90			10	0.90298
1742			88	11	0.923564
1744			88	11	0.923564
1777			88	11	0.923564
1778	88		11		0.923564
1780	88		11		0.923564
1787	90	10			0.90298
1789	10			90	0.90298
1795	90	10			0.90298
1798	90	10			0.90298
1799	90		10		0.90298
2014	25	75			1.0
2017			75	25	1.0
2018	75			25	1.0
2021	77	11	11		0.968132
2023	77			22	0.999999
2025	22			77	0.999999
2027	12	87			0.992554
2028	87		12		0.992554
2029		12		87	0.992554
2030	87	12			0.992554
2033			11	88	0.923564
2034			11	88	0.923564
2035			88	11	0.923564
2036		11	88		0.923564
2037	11		88		0.923564
2038		11		88	0.923564
2041	88	11			0.923564
2063	90	10			0.90298
2066			10	90	0.90298
2067	10			90	0.90298
2068	10		90		0.90298
2069		90		10	0.90298
2076	10			90	0.90298
2123			90	10	0.90298
2135	90			10	0.90298
2136	10			90	0.90298
2203		33		66	1.0
2223		66	33		1.0
2269			72	27	1.0
2276		27	72		1.0
2282	27			72	1.0
2284			37	62	1.0
2292	62	37			1.0
2363		80		20	0.995493
2392	50	50			1.0
2394	25	25		50	0.995533
2395	50	50			1.0
2396		50		50	1.0
2397	25	50	25		0.995533
2398	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain