SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene18

Length: 2738 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene18
ATGGGGGAATGAGCAAATAAATCACAAAGCGTCGTGACCTTGGCTGTCACTCTTCCAACACCATTGTTGGGCATCCCGCTGCAACTAGACAGCAGTATCTAGCTGCAACTAGAGCAGCAGTACCTAACGATGGCCATGTCACTACAATTGCAACTACTACATGGCTCTCAATCCTTTCAATCTCTACCTGAAAACCATCCCCACCATGGATTTCAAAGACTATTTCTCGGAAAAAATTTATTTGGCAAATCTCTGTTTAAGTCTAGCGTTATTAAAAGTTCTTCGAAGAGTGTGGATGCTGGGAAACACCACAAACAGTTGAAGCTGAGTACCAGATGTTCAGTCTCAACGACAGAGCCTTCGACAGCTGAAAATCAAAAAAAGAATCAGCTGAAAGCAAGGAGAGATGTAAGAAACATAGCAATTGTTGCTCATGTAGATCATGGGAAGACAACTTTAGTGGATGCAATGTTAAAGCAAGCCAAGGTGTTTCGTGTCAATCAATCTGTTCAAGAAAGAATTATGGACTCCAATGCACTGGAGAGAGAGCGGGGCATAACAATACTCAGCAAGAATACAGCAATATGCTACAAGGATACAAAGATTAACATCATTGATACTCCAGGTCATTCAGACTTTGGAGGGGAAGTGGAACGTGTGCTTAACATGGTGGAAGGTGTCCTTCTTGTGGTGGACTCTGTTGAGGGACCAATGCCACAAACACGATTTGTTTTAAAGAAAGCTCTAGAGCTTGGACTTTCTGTTCTAGTGGTGGTGAATAAGATTGACAGGCCTTCAGCTCGTCCTGATCATGTGGTCAATGCAACGTTTGAGCTTTTTATTGAATTGAATGCTACTGATGAACAGTGTGATTTCCAAGTCATATATGCAAGTGGTGTCAAGGGCCAAGCTGGATTAAGTCCTGAAAAAGTTGGGAATGACCTTGGACCTCTTTTTGAAGCAGTCCTTCGGTGCATACCTGAGCCGACTATTGATCCTGATAGTTATTTGCAGATGCTGGTAACGAATTTGGATTATGATGAACACAAAGGACGAATAGCTATTGGAAGAGTGCATGCTGGTAAAATGCAGAGGGGTATGGAGGTAAAGGTGTGTACACCAGATGAAAATTGCAGGTTTGGCAAAGTTAGTGAGGTCTTTGTTTACGAGAATTTTATGAGAGTCCCAGCTGAAACAGTAGAAGCTGGAGATATCTGTGCCGTCTGTGGTATTGATGATGTCCTTATTGGTGAGACATTGGCAGATAAAGTTAATGGCTTAGCTTTGCCTGGAATAAAAGTTGAGGAACCAACTGTAAGGATGTCATTTTCGATCAACACTTCACCATTTGTTGGCCGTGAGGGGAAACATGTGACAAGCAGAAAACTTCGTGAAAGGCTTTATCGTGAACTTGAGAGAAACTTGGCAATGAAAGTTGAAGATGGGGAGATGGCTGATACATTCATTATCAGTGGGAGGGGTACATTACACATAACCATACTGATTGAGAACATGCGGAGGGAAGGATATGAATTTATGGTGGGCCCACCAAAGGTTATAAACAAAAGACTAAATGACAAGCTGCTTGAACCCTATGAGATTGCTATTGTGGAGGTGCCAGAAGAATACATGGGTTCTGTTGTTGAACTTCTGGGAAAACGTCGTGGTGTGATGGTCGATATGCAAACTGGTGTGGAGAATACAACAGTGGTGAAATACAAAATACCAACTCGTGGTCTACTTGGTCTTCGAAATGCGATTTTGACTGCTTCTAGAGGCACTGCAGTTCTAAACACTGTTTTTGATGAGTATGGACCATGGGCCGGAGACATCAGTACAAGGGACCAAGGCTCTCTGGTCTCATTTGAGACAGGAACAAGTACTTCATATGCCTTATCCAGTTCTCAAGAAAGAGGCCAGTTGTTTTTAGGTCCTGGTGTAGATGTCTACAAGGGTCAAATTGTAGGTATCCATCAAAGAACAGGCGACCTGTCCCTAAATGTTTGCAAGAGGAAGGCTGCAACAAATGTCCGTTCAAACAAGGAACAGACAGTGGTCCTTAATGCACCTTTGGATTTCAGTCTGGATGATTGTATTGAATATATCCAAGAGGATGAACTAGTTGAAGTCACACCATTGAGCGTGCGCATGTGCAAGAACCCAAGGGCAATGAAGAAAACAAAATAACTTTCACCATTAACATACTACAATAAATATATCAAATATTTACACCATAAAATACTTTTTCACCTGTATAATCAGTGGTCTTGACTCTTTATTACGTTATCCAGGATCTGAAGACGGACTATCTAGATTTCCCAAGCAGTAGGTAACCTTATGGGTTGTAGATAAAAAGATTTGTCTTGGATTAATACCTCTCTCAATTCTGATTTTCTAGTAACCTAGAGTTTTACACCACTGGAAGAGTTGGGAACTTTGGGTTTCTCCCAAAGTCAGAGATCAGAGTGCCTTGTGTGTAGTTCAAGGTTTGACTTGACCTATTAGAGTCTCATTCATTGCTTTTGTATATGAAATTTCATTGCCATATTGTTATTACGTGTTAGTGGTGAACTGGCAGCAAAACAGCCGAGGAAGACTAATAAGCTGCTGGCCCATAACTTTAGGCATTTTCGATTATGAGATGCTGTTAGAAGAAGTAAAAGTTTATTTAAATTACATGGCTAAAAGCCATATTTCATGATTTAATGGAAAAGGTTATGGGGCTTCATTTTTGTTTC

Ace file of the UniGene sp_v2.0_unigene18 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	GTP-binding protein TypA [Trifolium pratense]	-	-	77%
FL-Next	sp=Translation factor GUF1 homolog, chloroplastic; Ostreococcus lucimarinus (strain CCE9901).	-	-	28%
Blast2go	elongation factor family protein	-	-	89%

Source	GOs	Term	Type	Identity
Blast2go	chloroplast RNA processing	GO:0031425	Biological Process	89%
Blast2go	translational elongation	GO:0006414	Biological Process	89%
Blast2go	GTP catabolic process	GO:0006184	Biological Process	89%
Blast2go	translation elongation factor activity	GO:0003746	Molecular Function	89%
Blast2go	GTPase activity	GO:0003924	Molecular Function	89%
Blast2go	GTP binding	GO:0005525	Molecular Function	89%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Translation elongation factor EFG/EF2, C-terminal	IPR000640	-	-
Sma3	Protein synthesis factor, GTP-binding	IPR000795	-	-
Sma3	Translation elongation factor EFTu/EF1A, domain 2	IPR004161	-	-
Sma3	Small GTP-binding protein domain	IPR005225	-	-
Sma3	GTP-binding protein TypA	IPR006298	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: A4RZA6

Fln msg: your sequence is longer than subject: 686 - 605

Fln protein: MAMSLQLQLLHGSQSFQSLPENHPHHGFQRLFLGKNLFGKSLFKSSVIKSSSKSVDAGKHHKQLKLSTRCSVSTTEPSTAENQKKNQLKARRDVRNIAIVAHVDHGKTTLVDAMLKQAKVFRVNQSVQERIMDSNALERERGITILSKNTAICYKDTKINIIDTPGHSDFGGEVERVLNMVEGVLLVVDSVEGPMPQTRFVLKKALELGLSVLVVVNKIDRPSARPDHVVNATFELFIELNATDEQCDFQVIYASGVKGQAGLSPEKVGNDLGPLFEAVLRCIPEPTIDPDSYLQMLVTNLDYDEHKGRIAIGRVHAGKMQRGMEVKVCTPDENCRFGKVSEVFVYENFMRVPAETVEAGDICAVCGIDDVLIGETLADKVNGLALPGIKVEEPTVRMSFSINTSPFVGREGKHVTSRKLRERLYRELERNLAMKVEDGEMADTFIISGRGTLHITILIENMRREGYEFMVGPPKVINKRLNDKLLEPYEIAIVEVPEEYMGSVVELLGKRRGVMVDMQTGVENTTVVKYKIPTRGLLGLRNAILTASRGTAVLNTVFDEYGPWAGDISTRDQGSLVSFETGTSTSYALSSSQERGQLFLGPGVDVYKGQIVGIHQRTGDLSLNVCKRKAATNVRSNKEQTVVLNAPLDFSLDDCIEYIQEDELVEVTPLSVRMCKNPRAMKKTK*

Protein Length: 687

Fln nts: ATGGGGGAATGAGCAAATAAATCACAAAGCGTCGTGACCTTGGCTGTCACTCTTCCAACACCATTGTTGGGCATCCCGCTGCAACTAGACAGCAGTATCTAGCTGCAACTAGAGCAGCAGTACCTAACG_-_ATGGCCATGTCACTACAATTGCAACTACTACATGGCTCTCAATCCTTTCAATCTCTACCTGAAAACCATCCCCACCATGGATTTCAAAGACTATTTCTCGGAAAAAATTTATTTGGCAAATCTCTGTTTAAGTCTAGCGTTATTAAAAGTTCTTCGAAGAGTGTGGATGCTGGGAAACACCACAAACAGTTGAAGCTGAGTACCAGATGTTCAGTCTCAACGACAGAGCCTTCGACAGCTGAAAATCAAAAAAAGAATCAGCTGAAAGCAAGGAGAGATGTAAGAAACATAGCAATTGTTGCTCATGTAGATCATGGGAAGACAACTTTAGTGGATGCAATGTTAAAGCAAGCCAAGGTGTTTCGTGTCAATCAATCTGTTCAAGAAAGAATTATGGACTCCAATGCACTGGAGAGAGAGCGGGGCATAACAATACTCAGCAAGAATACAGCAATATGCTACAAGGATACAAAGATTAACATCATTGATACTCCAGGTCATTCAGACTTTGGAGGGGAAGTGGAACGTGTGCTTAACATGGTGGAAGGTGTCCTTCTTGTGGTGGACTCTGTTGAGGGACCAATGCCACAAACACGATTTGTTTTAAAGAAAGCTCTAGAGCTTGGACTTTCTGTTCTAGTGGTGGTGAATAAGATTGACAGGCCTTCAGCTCGTCCTGATCATGTGGTCAATGCAACGTTTGAGCTTTTTATTGAATTGAATGCTACTGATGAACAGTGTGATTTCCAAGTCATATATGCAAGTGGTGTCAAGGGCCAAGCTGGATTAAGTCCTGAAAAAGTTGGGAATGACCTTGGACCTCTTTTTGAAGCAGTCCTTCGGTGCATACCTGAGCCGACTATTGATCCTGATAGTTATTTGCAGATGCTGGTAACGAATTTGGATTATGATGAACACAAAGGACGAATAGCTATTGGAAGAGTGCATGCTGGTAAAATGCAGAGGGGTATGGAGGTAAAGGTGTGTACACCAGATGAAAATTGCAGGTTTGGCAAAGTTAGTGAGGTCTTTGTTTACGAGAATTTTATGAGAGTCCCAGCTGAAACAGTAGAAGCTGGAGATATCTGTGCCGTCTGTGGTATTGATGATGTCCTTATTGGTGAGACATTGGCAGATAAAGTTAATGGCTTAGCTTTGCCTGGAATAAAAGTTGAGGAACCAACTGTAAGGATGTCATTTTCGATCAACACTTCACCATTTGTTGGCCGTGAGGGGAAACATGTGACAAGCAGAAAACTTCGTGAAAGGCTTTATCGTGAACTTGAGAGAAACTTGGCAATGAAAGTTGAAGATGGGGAGATGGCTGATACATTCATTATCAGTGGGAGGGGTACATTACACATAACCATACTGATTGAGAACATGCGGAGGGAAGGATATGAATTTATGGTGGGCCCACCAAAGGTTATAAACAAAAGACTAAATGACAAGCTGCTTGAACCCTATGAGATTGCTATTGTGGAGGTGCCAGAAGAATACATGGGTTCTGTTGTTGAACTTCTGGGAAAACGTCGTGGTGTGATGGTCGATATGCAAACTGGTGTGGAGAATACAACAGTGGTGAAATACAAAATACCAACTCGTGGTCTACTTGGTCTTCGAAATGCGATTTTGACTGCTTCTAGAGGCACTGCAGTTCTAAACACTGTTTTTGATGAGTATGGACCATGGGCCGGAGACATCAGTACAAGGGACCAAGGCTCTCTGGTCTCATTTGAGACAGGAACAAGTACTTCATATGCCTTATCCAGTTCTCAAGAAAGAGGCCAGTTGTTTTTAGGTCCTGGTGTAGATGTCTACAAGGGTCAAATTGTAGGTATCCATCAAAGAACAGGCGACCTGTCCCTAAATGTTTGCAAGAGGAAGGCTGCAACAAATGTCCGTTCAAACAAGGAACAGACAGTGGTCCTTAATGCACCTTTGGATTTCAGTCTGGATGATTGTATTGAATATATCCAAGAGGATGAACTAGTTGAAGTCACACCATTGAGCGTGCGCATGTGCAAGAACCCAAGGGCAATGAAGAAAACAAAATAA___CTTTCACCATTAACATACTACAATAAATATATCAAATATTTACACCATAAAATACTTTTTCACCTGTATAATCAGTGGTCTTGACTCTTTATTACGTTATCCAGGATCTGAAGACGGACTATCTAGATTTCCCAAGCAGTAGGTAACCTTATGGGTTGTAGATAAAAAGATTTGTCTTGGATTAATACCTCTCTCAATTCTGATTTTCTAGTAACCTAGAGTTTTACACCACTGGAAGAGTTGGGAACTTTGGGTTTCTCCCAAAGTCAGAGATCAGAGTGCCTTGTGTGTAGTTCAAGGTTTGACTTGACCTATTAGAGTCTCATTCATTGCTTTTGTATATGAAATTTCATTGCCATATTGTTATTACGTGTTAGTGGTGAACTGGCAGCAAAACAGCCGAGGAAGACTAATAAGCTGCTGGCCCATAACTTTAGGCATTTTCGATTATGAGATGCTGTTAGAAGAAGTAAAAGTTTATTTAAATTACATGGCTAAAAGCCATATTTCATGATTTAATGGAAAAGGTTATGGGGCTTCATTTTTGTTTC

Fln Alignment:

Contig18___LKARRDVRNIAIVAHVDHGKTTLVDAMLKQAKVFRVNQSVQERIMDSNALERERGITILSKNTAICYKDTK-----INIIDTPGHSDFGGEVERVLNMVEGVLLVVDSVEGPMPQTRFVLKKALELGLSVLVVVNKIDRPSARPDHVVNATFELFIELNATDEQCDFQVIYASGVKGQAGLSPEKVGNDLGPLFEAVLRCIPEPTIDPDSYLQMLVTNLDYDEHKGRIAIGRVHAGKMQRGMEVKVCTPDENCRFGKVSEVFVYENFMRVPAETVEAGDICAVCG----IDDVLIGETLADKVN--GLALPGIKVEEPTVRMS-FSINTSPFVGREGKHVTSXXXXXXXXXXXXRNLAMKVE-DGEMADTFIISGRGTLHITILIENMRRE-GYEFMVGPPKVINK--------------------RLNDKLLEPYEIAIVEVPEEYMGSVVELLGKRRGVMVDMQTGVENTTVVKYKIP
A4RZA6____________LPARR-IRNFSIIAHIDHGKSTLADTLLMRTKTVKERDMVKQ-VLDSMDLERERGITIKLNSARMDYVASDGELYVLNLIDTPGHVDFSYEVSRSLAACEGALLVVDSSQGVEAQTVANVYLALENDLEIFTVLNKIDLAGSEPDR-VKQEIEDVLGLDASD----------------AILASAKANIGMDDILERIVQVVPPPRDTANEPLRALIFDSYFDPYRGVVAVFRVMDGTMKTGDAMKMMATGATFAADEIGIM----RPDKVPVKELGPGEVGYIIGGIKSVADARVGDTITLSKNPAAEALPGYSKSVPMVFAGIFPTDTDRY--------DDLRESLQRLQINDCSLSFEPEQNSAMGTGFRCGFLGLLHMEIIQERLEREYDLDLITTAPSVVYNVYRTDGTMDSISNPADLCPPELRERIEEPYCRLDMIAPSDYVGTLMELASQRRGEFIDMTYLSETRTSIKYDIP

SNPs (tot: 58)

Position	A %	C %	G %	T %	Probability
1395		12		87	0.992554
1736			18	81	0.999994
1739		90	10		0.90298
1741		10	90		0.90298
1742	10		90		0.90298
1743	90		10		0.90298
1744		10		90	0.90298
1748			10	90	0.90298
1753	10		90		0.90298
1754		10	90		0.90298
1755		90	10		0.90298
1756	10			90	0.90298
1758	90		10		0.90298
1897	81			18	0.999994
2131	18			81	0.999994
2132		18		81	0.999994
2134	80			20	0.999996
2167			16	83	0.994512
2295	80		20		0.995493
2332	25			75	0.996473
2333		75		25	0.996473
2334	25			75	0.996473
2337	25	75			0.996473
2340	25			75	0.996473
2341		25	75		0.996473
2345	25			75	0.996473
2451	10	90			0.90298
2475	90		10		0.90298
2489	90	10			0.90298
2496	85	14			0.993533
2497	85	14			0.993533
2506	16			83	0.994512
2507	16			83	0.994512
2508	16			83	0.994512
2509	16	83			0.994512
2568	11			88	0.923564
2627		87	12		0.992554
2632			12	87	0.992554
2633	12		87		0.992554
2638		12		87	0.992554
2641	87		12		0.992554
2645			12	87	0.992554
2646	14		85		0.993533
2648			14	85	0.993533
2683		16	83		0.994512
2684		16	83		0.994512
2685	83	16			0.994512
2687			83	16	0.994512
2690	16	16		66	0.988813
2691	33	16	50		1.0
2692	66	16		16	0.988813
2696	20		80		0.995493
2697	20	60		20	0.99255
2698	20	60		20	0.99255
2699	75			25	0.996473
2706	33	66			0.997452
2707			75	25	0.996473
2709		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain