SustainPineDB

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Assembly info
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Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene83257

Length: 390 nt

Origin of reads: Biogeco1 v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene83257
CGTCGTGACCTTGGCTGTCACTCATTCTTGCATGGGGACTTGCATGAAAAAATCTATATGGAACAACCTTCTGGCTGTATACAAACATACTCTAGCCTTGTATGTCGGCTTAAGAAATCCCTTAATGGTCTCAAGCAAGCCCCTCAGGCTTGGTATGCCAAAATGTATAACTTTCTTTTAGATATTGGCTTTTCTAGGTGTCATTCTGACAAGATTGTCTACACCAAGAAAGTGGGTAAGTCTCTTATCATTTGATGATCTTATCCTTACTGGTAGTGATCCCAACCTTATAAACCATGTGAAGTCCAACCTTAAAAAGCAGTGTGAAATGACAAACTTAGGACACTTGCATTATTCTCTTGGTCTTCAAGTCTTGCAATCCAAGAAAGG

Ace file of the UniGene sp_v2.0_unigene83257 (antisense)

If you push the Download ace button, you will download an ace file of this UniGene.

To watch this ACE file you will need an ACE viewer program as Tablet:

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	RVT_2 domain containing protein	-	-	2.0e-27	49%
FL-Next	tr=Reverse transcriptase; Picea glauca (White spruce) (Pinus glauca).	-	-	0.0	46%
Blast2go	reverse transcriptase	-	-	6.73647e-22	65%

Source	InterPros	Term	Type	Identity
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Pleckstrin homology domain	IPR001849	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3		IPR013084	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: Q9M5J7

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 233 aas, your sequence is shorter than subject: 85 - 542

Fln protein: RRDLGCHSFLHGDLHEKIYMEQPSGCIQTYSSLVCRLKKSLNGLKQAPQAWYAKMYNFLLDIGFSRCHSDKIVYTKKVGKSLII*

Protein Length: 86

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTCTTGCATGGGGACTTGCATGAAAAAATCTATATGGAACAACCTTCTGGCTGTATACAAACATACTCTAGCCTTGTATGTCGGCTTAAGAAATCCCTTAATGGTCTCAAGCAAGCCCCTCAGGCTTGGTATGCCAAAATGTATAACTTTCTTTTAGATATTGGCTTTTCTAGGTGTCATTCTGACAAGATTGTCTACACCAAGAAAGTGGGTAAGTCTCTTATCATTTGATGATCTTATCCTTACTGGTAGTGATCCCAACCTTATAAACCATGTGAAGTCCAACCTTAAAAAGCAGTGTGAAATGACAAACTTAGGACACTTGCATTATTCTCTTGGTCTTCAAGTCTTGCAATCCAAGAAAGG

Fln Alignment:

AllPineV4_rep_c143691___SFLHGDLHEKIYMEQPSG-CIQTYSSLVCRLKKSLNGLKQAPQAWYAKMYNFLLDIGFSRCHSDKIVYTKKVGKSLI
Q9M5J7_______________TFLHGDLEEEIYMKQPEGFVVKGNKELVCKINKSLCGVKQSPRMWYQKFDTYILRLGFVISRADHCVYSKQVGNHFI

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain