SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene82847

Length: 938 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene82847

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Tubulin alpha-6 chain n=85 Tax=Eukaryota RepID=TBA6_ARATH	-	-	87%
FL-Next	tr=Alpha tubulin 1; Pseudotsuga menziesii var. menziesii.	-	-	100%
Blast2go	alpha tubulin 1	-	-	99%

Source	InterPros	Term	Type	Identity
Sma3	Tubulin	IPR000217	-	-
Sma3	Alpha tubulin	IPR002452	-	-
Sma3	Tubulin/FtsZ, GTPase domain	IPR003008	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3	Tubulin, conserved site	IPR017975	-	-
Sma3	Tubulin/FtsZ, 2-layer sandwich domain	IPR018316	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: Q5ME66

Fln msg: Separated hits, possible frame ERROR between 441 and 459, and possible frame ERROR between 563 and 593, Unexpected stop codon in the beginning of your sequence, STOP codon was not found. Distance to subject end: 0 aas, your sequence is shorter than subject: 313 - 451

Fln protein: MGDG*ALRIRQHKTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPxxxxxxxSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICxxxxxxxxxxxTK*TIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEACEDLAALEKDYEEVGAESAEGEEGDEGDEY

Protein Length: 314

Fln nts: ATGGGGGACGGCTGAGCTCTTCGAATACGGCAGCACAAAACTGTCGGTGGCGGAGACGATGCATTCAACACTTTCTTCAGCGAAACTGGAGCCGGAAAGCATGTTCCCCGCGCTGTCTTTGTAGATCTGGAGCCGACTGTCATTGACGAGGTACGGACTGGTACTTACCGTCAGCTTTTCCATCCTGAACAGCTTATCAGCGGCAAGGAAGATGCGGCCAACAACTTTGCCCGTGGTCATTACACCATTGGCAAGGAAATTGTGGACCTCTGCCTGGACCGTATTCGGAAGCTGGCTGACAACTGCACGGGTCTTCAAGGATTCCTCGTCTTCAACGCAGTTGGTGGAGGAACTGGTTCTGGTCTTGGTTCTCTTCTACTGGAACGTCTTTCAGTGGACTACGGCAAAAAATCCAAACTTGGATTCACTGTGTATCCATCxxxxxxxxxxxxxxxxxxTTCGGTCGTTGAGCCGTACAACAGCGTTCTCTCCACGCATTCTCTTCTGGAACACACCGATGTGGCCGTTCTCCTCGATAATGAAGCTATTTACGATATTTGCCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAGACAAAGTGAACCATCCAGTTTGTTGATTGGTGTCCCACAGGATTTAAATGCGGAATCAACTACCAGCCTCCCACCGTTGTCCCCGGAGGCGATCTCGCCAAGGTTCAGAGAGCCGTGTGCATGATTTCCAACTCGACCAGTGTTGCAGAGGTGTTCTCGAGAATCGATCATAAGTTCGATCTCATGTATGCCAAGCGAGCATTTGTTCATTGGTATGTGGGCGAGGGTATGGAAGAGGGCGAGTTTTCTGAAGCGTGTGAGGATCTTGCTGCCCTCGAGAAGGACTATGAGGAGGTTGGCGCTGAGTCTGCAGAGGGAGAGGAAGGTGATGAGGGAGACGAGTACT

Fln Alignment:

AllPineV4_rep_c142508___KTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSxxxxxxSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRxxxxxxxxxxTK*TIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEACEDLAALEKDY
Q5ME66_______________KTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSxxxxxxSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRxxxxxxxxxxTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY

SNPs (tot: 3)

Position	A %	C %	T %	Probability
230	33	66		0.997452
231	33	66		0.997452
232		66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain