SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene81328

Length: 896 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene81328 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cysteine protease, putative n=1 Tax=Ricinus communis RepID=B9RHA4_RICCO	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Blast2go	cysteine proteinase rd19a	-	-	72%

Source	GOs	Term	Type	Identity
Blast2go	defense response to bacterium	GO:0042742	Biological Process	72%
Blast2go	response to desiccation	GO:0009269	Biological Process	72%
Blast2go	response to salt stress	GO:0009651	Biological Process	72%
Blast2go	vacuole	GO:0005773	Cellular Component	72%
Blast2go	nucleus	GO:0005634	Cellular Component	72%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Peptidase C1A, papain	IPR013128	-	-
Sma3	Peptidase C1A, papain C-terminal	IPR000668	-	-
Sma3	Proteinase inhibitor I29, cathepsin propeptide	IPR013201	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NKS7

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 52 aas, your sequence is shorter than subject: 260 - 366

Fln protein: MGSSTLLLLVLCISSVICLSLAIRPDDDLIRQVTDEVDSDPQILDARSALFNAEAHFRRFIRRYGKKYSGPEEHEHRFGVFKSNLLRALEHQKLDPQASHGVTEFSDLTQEEFDWREKGAVTEVKNQGSCGSCWAFSTTGALEGANFLKTGKLVSLSEQQLVDCDHECDPSDARSCDSGCNGGLMTSAYQYALKAGGLEREEDYPYTGKDGTCSFNKNKIVAHVSNFSVVSIDEDQIAANLVKNGPLSVGIKNTHLLFC*

Protein Length: 261

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTATCTGTAATTGCTCAACA_-_ATGGGATCCTCCACGCTGCTGCTTTTGGTTCTCTGTATTTCCTCTGTAATTTGCCTTTCCTTGGCCATAAGGCCTGACGATGACCTTATTCGTCAAGTGACGGACGAAGTAGATTCAGATCCACAGATCCTCGATGCTCGGAGCGCCCTGTTCAACGCTGAAGCGCACTTCAGGCGCTTCATCAGGCGCTACGGTAAGAAGTACTCGGGGCCAGAAGAGCACGAGCACCGCTTCGGTGTCTTCAAGAGCAATTTACTAAGAGCCTTGGAGCACCAGAAGCTCGACCCCCAGGCCTCCCATGGCGTCACCGAATTCTCTGATTTGACACAAGAGGAGTTCGATTGGAGAGAGAAGGGAGCCGTGACCGAGGTTAAGAATCAGGGATCTTGCGGTTCCTGCTGGGCTTTCAGCACAACCGGGGCGCTAGAGGGCGCGAATTTCCTGAAGACGGGGAAGCTGGTGAGCCTGAGCGAGCAACAATTGGTGGACTGTGATCACGAGTGCGATCCTTCGGACGCAAGATCATGTGATTCTGGTTGCAATGGTGGGCTAATGACAAGTGCTTATCAATATGCTCTGAAAGCTGGTGGATTGGAGAGGGAAGAGGACTACCCATACACTGGAAAAGACGGAACTTGCAGCTTCAACAAGAACAAAATTGTCGCACATGTTTCGAATTTCAGCGTTGTTTCTATTGATGAAGATCAAATTGCTGCAAATCTGGTGAAGAATGGACCTCTATCAGTGGGAATCAAAAATACGCACCTACTATTTTGTTAGTTTCTTAGAGAAAGATTACACTTTTATTGCAGAAACCCTATGCAATTTAATAAGCATCTTTTGTCACTTATT

Fln Alignment:

AllPineV4_rep_c136259___AIRPDDDLIRQVTDEVDSDPQILDARSALFNAEAHFRRFIRRYGKKYSGPEEHEHRFGVFKSNLLRALEHQKLDPQASHGVTEFSDLTQEEF------------------------------DWREKGAVTEVKNQGSCGSCWAFSTTGALEGANFLKTGKLVSLSEQQLVDCDHECDPSDARSCDSGCNGGLMTSAYQYALKAGGLEREEDYPYTGKDGTCSFNKNKIVAHVSNFSVVSIDEDQIAANLVKNGPLSVGI
A9NKS7_______________ATRPDDDLIRQVTDEVVSDPQILDARSALFNAEVHFRHFIRRYGKKYSGPEEHEHRFGVFKSNLLRALEHQKLDPRASHGVTKFSDLTQEEFRHQYLGLRAPPLRDAHDAPILPTNDLPEDFDWREKGAVTEVKNQGSCGSCWAFSTTGALEGANFLKTGELVSLSEQQLVDCDHECDPSDARSCDSGCNGGLMTSAYQYALKSGGLEKEEDYPYTGKDGTCSFNKNKIVAHVSNFSVVSIDEGQIAANLVKNGPLSVGI

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
521		66		33	0.997452
525	66			33	0.997452
526		66	33		0.997452
527	33			66	0.997452
529	33	66			0.997452
530			33	66	0.997452
532		33		66	0.997452
533	33			66	0.997452
534	20		80		0.995493
535			20	80	0.995493
540	80	20			0.995493
541	80		20		0.995493
542	20			80	0.995493
553	25		75		0.996473
628	20		80		0.995493
717		66		33	0.997452
879		33		66	0.997452
884		0	33	33	0.998258
885		25	75		0.996475
886	75	25			0.996475
887		25	50	25	0.995556
888	25	25		50	0.995539
889		25	50	25	0.995556
890	66	33			0.997454
893			66	33	0.997461

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain