SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene76810

Length: 1715 nt

Origin of reads: Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene76810 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Aconitate hydratase, cytoplasmic n=8 Tax=rosids RepID=ACOC_CUCMA	-	-	82%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	aconitate hydratase	-	-	91%

Source	ECs	Term	Type	e value	Identity
Blast2go	Aconitate hydratase.	EC:4.2.1.3	-	0.0	91%

Source	KEGGs	Term	Identity
Blast2go	Citrate cycle (TCA cycle)	00020	91%
Blast2go	Glyoxylate and dicarboxylate metabolism	00630	91%
Blast2go	Carbon fixation pathways in prokaryotes	00720	91%
Blast2go	Biosynthesis of phenylpropanoids	01061	91%
Blast2go	Biosynthesis of terpenoids and steroids	01062	91%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	91%
Blast2go	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	91%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	91%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	91%
Blast2go	Biosynthesis of plant hormones	01070	91%
Blast2go	Metabolic pathways	01100	91%
Blast2go	Biosynthesis of secondary metabolites	01110	91%

Source	GOs	Term	Type	Identity
Blast2go	cellular response to iron ion	GO:0071281	Biological Process	91%
Blast2go	response to salt stress	GO:0009651	Biological Process	91%
Blast2go	cellular response to fatty acid	GO:0071398	Biological Process	91%
Blast2go	cellular response to potassium ion starvation	GO:0051365	Biological Process	91%
Blast2go	isocitrate metabolic process	GO:0006102	Biological Process	91%
Blast2go	cellular response to nitric oxide	GO:0071732	Biological Process	91%
Blast2go	citrate metabolic process	GO:0006101	Biological Process	91%
Blast2go	copper ion binding	GO:0005507	Molecular Function	91%
Blast2go	mRNA 5'-UTR binding	GO:0048027	Molecular Function	91%
Blast2go	aconitate hydratase activity	GO:0003994	Molecular Function	91%
Blast2go	mitochondrion	GO:0005739	Cellular Component	91%
Blast2go	vacuole	GO:0005773	Cellular Component	91%
Blast2go	plasma membrane	GO:0005886	Cellular Component	91%
Blast2go	apoplast	GO:0048046	Cellular Component	91%

Source	InterPros	Term	Type	Identity
Sma3	G-patch domain	IPR000467	-	-
Sma3	Aconitase A/isopropylmalate dehydratase small subunit, swivel	IPR000573	-	-
Sma3	Oxysterol-binding protein	IPR000648	-	-
Sma3	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	IPR001030	-	-
Sma3	Aconitase/iron regulatory protein 2	IPR006249	-	-
Sma3	Aconitase/3-isopropylmalate dehydratase, swivel	IPR015928	-	-
Sma3	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	IPR015931	-	-
Sma3	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2	IPR015932	-	-
Sma3	Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase	IPR015934	-	-
Sma3	Aconitase/isopropylmalate dehydratase	IPR015937	-	-
Sma3	Aconitase family, 4Fe-4S cluster binding site	IPR018136	-	-
Sma3	PDZ-binding protein, CRIPT	IPR019367	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NX49

Fln msg: Warning!, your query overlaps and the subject is separated, Distance to subject end: 69 aas, your sequence is shorter than subject: 420 - 565

Fln protein: MGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSDETVKMIEAYLRANKMFVDYNEPQIERSYSSYLELDLSSVEPCISGPKRPHDRVTLKEMKSDWHACLDNKVGFKGFAIPKEKQEKVVKFTYNGAPAELRHGDVVIAAITSCTNTSNPNVMLGAGLVAKKACELGLEVKPWIKTSLAPGSGVVTKYLKRSGLDKYLDQQGFQLVGYGCTTCIGNSGDLHESVSSAIAENDLVASAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFETEPIGTGKDGKKVYFRDVWPSTEEIAEVVQSAVLPDMFKQTYEAITKGNPMWNQLPVPTGTLYTWDPKSTYIHDPPYFKxxxxxSSRDWAAKGPMLQGVKAVISKSFERIHRSNLVGMGIIPLCFKSGEDAES

Protein Length: 421

Fln nts: CGTCGTACCTTGCGTCATCAATTGCCTGCAACTCAAGTTTGAATTTAATCGTAACACAGAAAGGTTTGCTTTCCTCAAGTGGGGCTCAACTGCTTTTGAGAACATGCTCGTTGTTCCTCCGGGTTCTGGTATTGTACACCAGGTGAATTTGGAATATCTGGGAAGGGTGGTATTTAATACCAATGGTCTACTTTATCCGGATAGTGTTGTTGGAACTGATTCCCACACAACTATGATAGATGGATTAGGTGTTGCTGGCTGGGGAGTTGGAGGAATCGAAGCAGAAGCTGCTATGCTTGGCCAGCCAATGAGTATGGTTCTTCCTGGTGTTGTTGGTTTCAAATTACTGGCACTTTGAACAATGGAGTGACAGCTACGGACTTGGTTCTAACGGTGACTCAAATTTTACGAAAGCATGGTGTGGTTGGTAAATTTGTTGAGTTCTATGGTGATGGC_-_ATGGGTGAATTATCACTTGCCGATCGAGCAACTATTGCAAACATGTCTCCAGAGTATGGGGCAACCATGGGATTTTTCCCTGTAGATCATGTTACTCTGCAGTATTTGAGGTTAACAGGTCGCAGTGATGAGACTGTGAAGATGATTGAGGCCTACCTCCGTGCCAACAAAATGTTTGTGGACTATAATGAGCCACAAATAGAGAGGTCTTATTCGTCCTACTTGGAATTGGATCTTTCATCTGTTGAACCTTGTATATCAGGACCAAAACGACCTCATGATCGGGTTACTCTCAAGGAAATGAAATCTGATTGGCATGCTTGCCTAGACAACAAGGTTGGGTTTAAGGGTTTTGCCATTCCGAAAGAGAAACAAGAAAAAGTTGTGAAGTTTACATACAATGGTGCACCTGCTGAACTAAGACATGGTGATGTTGTTATAGCGGCTATCACCAGTTGCACGAACACATCAAATCCTAATGTCATGCTTGGGGCTGGCCTTGTTGCAAAGAAAGCTTGTGAATTAGGTCTGGAGGTCAAGCCATGGATTAAGACCAGTCTTGCCCCAGGATCTGGAGTTGTTACTAAATATTTGAAGCGGAGCGGCCTGGATAAGTACTTAGATCAACAAGGCTTTCAACTTGTTGGGTATGGATGTACTACATGTATTGGAAACTCAGGAGATCTTCATGAATCTGTGAGCTCTGCTATTGCTGAAAATGATTTAGTTGCGTCAGCTGTGCTGTCTGGCAACAGAAATTTTGAAGGCCGTGTTCATCCTCTGACTCGGGCAAACTACCTCGCTTCTCCTCCACTTGTAGTTGCATATGCTCTTGCTGGCACTGTTGACATTGATTTCGAGACGGAGCCTATTGGTACTGGAAAGGATGGGAAGAAAGTTTACTTCAGAGATGTCTGGCCTTCAACTGAAGAAATAGCAGAGGTTGTACAATCTGCTGTCCTACCAGACATGTTTAAGCAAACATATGAAGCAATAACAAAGGGCAATCCTATGTGGAACCAGCTTCCTGTTCCAACCGGCACTTTGTATACGTGGGATCCAAAATCCACTTACATACATGATCCTCCATATTTTAAAxxxxxxxxxxxxxxxAGTTCTCGGGATTGGGCTGCAAAGGGTCCAATGTTGCAGGGTGTGAAAGCTGTTATCTCAAAAAGTTTTGAGCGTATTCATCGCAGCAATTTGGTTGGCATGGGTATTATTCCATTGTGTTTCAAATCTGGTGAGGACGCTGAGTCG

Fln Alignment:

AllPineV4_rep_c112325___MIEAYLRANKMFVDYNEPQIERXXXXXXXXXXXSVEPCISGPKRPHDRVTLKEMKSDWHACLDNKVGFKGFAIPKEKQEKVVKFTYNGAPAELRHGDVVIAAITSCTNTSNPNVMLGAGLVAKKACELGLEVKPWIKTSLAPGSGVVTKYLKRSGLDKYLDQQGFQLVGYGCTTCIGNSGDLHESVSSAIAENDLVASAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFETEPIGTGKDGKKVYFRDVWPSTEEIAEVVQSAVLPDMFKQTYEAITKGNPMWNQLPVPTGTLYTWDPKSTYIHDPPYFKDMVVxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYGSGSSRDWAAKGPMLQGVKAVISKSFERIHRSNLVGMGIIPLCFKSGEDAES
A9NX49_______________MIESYLRANKMFVDYNEPQIERTYSSYLELDLSSVEPCISGPKRPHDRVTLKEMKADWHSCLDNKVGFKGFAIPKEKQEKVVKFTYNGTPAELRHGDVVIAAITSCTNTSNPNVMLGAGLVAKKACELGLEVKPWVKTSLAPGSGVVTKYLKKSGLDKYLDQQGFQLVGYGCTTCIGNSGDLHESVSSAITENDLVASAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFETEPIGTGKDGKKVYFRDIWPSTEEIAEVVHSAVLPDMFRQTYEAITKGNPMWNQLPVPTGTLYTWDPTSTYIHDPPYFKDMTMxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYGSGSSRDWAAKGPMLQGVKAVISKSFERIHRSNLVGMGIIPLCFKSGEDAES

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
1438		20		80	0.995494
1439		20		80	0.995494
1580		83	16		0.994512
1583		83	16		0.994512
1586	16		83		0.994512
1587		16		83	0.994512
1588	16		83		0.994512
1589			16	83	0.994512
1590	83	16			0.994512
1594			14	85	0.993534
1595	85		14		0.993534
1596		85		14	0.993534
1599		14	85		0.993534
1600	85		14		0.993534
1603		85	14		0.993535
1605			80	20	0.995493
1609			80	20	0.995493
1610	80		20		0.995493
1613		80	20		0.995493
1615	66		16		0.988813
1616		66		16	0.988813
1621		20	80		0.995493
1625	40			60	1.0
1627			33	66	1.0
1630	33			66	1.0
1633	60		40		1.0
1635	60	40			1.0
1640	25			75	0.996477
1641		33	33		0.997753
1644			75	25	0.996476
1648		66	33		0.997476
1651	33	66			0.997476
1653		66	33		0.997458
1654			33	66	0.997458
1656		33	66		0.997458
1658		33	66		0.997458
1662	66	33			0.997459

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain