SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene76784

Length: 2741 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene76784
CGTCGTGACCTTGGCTGTCACTCAATTTGCATTCCAATATTTGCTGCTTGGGATTGAACCGGCCAGGGATGATCCAAACCCTCACTCTACAAAACCTAGGACGATCCAGAGCTCTGGATATTGTGTCTGGTTCTGCGCTAAATCGGCTTAATTCGATCCACGGAAATGCTTTGCGACTAACGCAGCATGTCGAAGCGAACACATTTATGTCCGATTCGCAGAAAACAGTGTACCATTTATTCCAAGTTTGCTAACAACTGCTGAATCCCGAAAACCGGTCAAGCAATTGTCGGTTTTAATTGGATCTGCAATCTGCTTTCTTAAATTGGCGGTCTTAGTGCAGAATGCATATTTGTGGCCTTTTGAGAACTTTTCTTTTGCTGTATAGGGTTTGCTTATAAATAATCTGCTTGGCGATTTGCGGGGATTAATCTTCTTAGCCGTGAATTAAAACGTTTCACTGTTAATTTCTTCTCCAAAGATGGCGGGAAGAGGGTTTCAGGGTATGAATTACAGTTACTGTCTGGCCGCGGTGTTATTGGTGATAATCTCTTTGACGACCATGGCTATGGCGAAATTTGTGGTGGAGAAGAACAGCTTGAAAGTTCTCTCTCCGGATAATATTAAGGGCACTTATGACAGTGCTATAGGCAATTTTGGGATCCCTCAGTACGGTGGAACCATGGTCGGTACGGCTGTTTATCCGAAGGAGAATCAGAATGGCTGTAAGACTTTCGATACTTTTAATATTGAGTTTAAATCGAAGCCGGGAGGGCTTCCTAATGTCGTGCTGGTTAACCGTGGAGATTGTTATTTTGCTTTGAAAGTTTGGAATGCTCAGAATGCTGGTGCAGCAGCTGTTCTTGTTTCTGATGATAGAGAAGAGCCATTGATAACTATGGATTCTCCTGAAGAAGATAGTTCGGCAGCGGAATATCTGCAGAATATCACCATTCCTTCTGCCCTTATTGAGAAAGAGTTTGGAGACAAGCTGAAGGGGGCTATCAACAAGGGTGACATGGTTAATATTAATTTAGACTGGAGGGAGTCTGTTCCTCATCCAGATGATCGTGTGGAGTATGAGTTGTGGACAAATAGCAATGATGAATGTGGGTCAAAGTGTGATACACAACTGGAATTTATAAAGAATTTTAAAGGAGCTGCCCAAATTCTTGAGAAAAGTGGTTACACACAATTCACTCCTCACTATATCACCTGGTACTGTCCCCAAGCATTTACCGTAAGTAAGCAATGCAAGGCTCAATGCATCAATCATGGAAGATACTGTGCACCTGATCCAGAACAGGATTTCAGTCGGGGTTATGATGGTAAAGATGTTGTACTTGAAAATCTGAGGCAATTGTGTATCTTCAAAGTTGCAAATGAAAGTAATAGACCCTGGGCATGGTGGGATTATGTCACTGATTTCCATATTAGATGTCCAATGAAGGAGAAAAAGTATACCAAGGAATGTGCAGAAAATGTGATCCAGTCTCTAGGTTTGGATAACAAAAAGATAGAAACATGCATGGGGGATCCAAATGCAGATGCAGAGAACCCAATTTTGAAACAAGAGCAAGATGCACAGGTTGGACATGGATCACGAGGTGATGTCACCATATTGCCCACTCTCATTATTAACAACAGACAATATAGAGGAAAACTGGACAAAGGGCCTGTGTTGAAGGCAATTTGTGCAGGCTTTCAGGAAACTACTGAGCCAACTGTATGCTTGAGTGATGATATGGAAACAAATGAATGTTTGAAAAATAATGGTGGTTGCTGGCAAGACAAGAAGTCAAATATAACTGCCTGCAAGGATACTTTTCGGGGAAGGGTTTGTGAGTGTCCCTTGGTAGACGGTGTTCAATTTAAGGGAGATGGTTATTCTAACTGTGAAGGTAAAAAGGCTGAAGAGGGAAAGGTTGGCTGGGCTGCTGTATGGGTTGTATTATTAGGTTTGGCCGCTGCTGCTGCTGGAGCTTATGTAGTTTACAAATACAGACTAAGATCATACATGGACTCTGAGATCAGGGCCATCATGGCACAATACATGCCTCTCGACAGCCAAGGAGAGATTCCTAACCATGTTAATAATGAGACGTTATAAAATTCTAGAGAGTCGAGACTCCTGTGTAATGACTCTTCATGTTCGTGGCTATTGTGAGTACATATTAGAAGTTCTCTGCTTGATCTTACAAAAGCTGTGCAAATTATAATGTTCTTACAATTCAAAGACATCCTTTGGGAAGCTCCTCATTCATGAACATTGTACCCACATTCAGACTGTATATTAAGAGTTGGGAAGAGGATTAGATATTAGCAATCCAGTTTCAGGTCTTTACTTGGTTGATATGGTTTCTTGACAGACAGATGACTTGGTTATCTTGTTGTAACTCCAGTTAGTCTTCAAAGTTCCAACATTACAGTTTTGCAGTATCTGAAAATGATTTATTAAAGGAGGTTGCTGATAGAGCAGAGCTTTTATTGAGTAGAAAGTCTACTTGAAGGCTAACACTGCCATGTTTCTGAGTATAGTTTAGTCACTTCCTTTAACTACATAGTCTTAAAGATGGTCTCTTTATCTAGAAATCTTTAAAAATTATTGGAGAATAAACATGTTGAAGGTTTACATCTTACACGAGGTCAATCTAGCATCTCCATCTTTTGCATGTTTTTGTGCAGTTCTTTGTTGTTATGATTATTTCAATTATCTGAAATTTCGTATTGTACCATACATT

Ace file of the UniGene sp_v2.0_unigene76784 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	PA domain containing protein	-	-	5.0e-12	30%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	94%
Blast2go	vacuolar-sorting receptor 3	-	-	0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	protein targeting to vacuole	GO:0006623	Biological Process	89%
Blast2go	Golgi transport complex	GO:0017119	Cellular Component	89%
Blast2go	membrane	GO:0016020	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	EGF-type aspartate/asparagine hydroxylation site	IPR000152	-	-
Sma3	EGF-like calcium-binding, conserved site	IPR018097	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	Protease-associated domain, PA	IPR003137	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPV3

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 93 aas, putative chimeric sequence, your sequence is shorter than subject: 543 - 635

Fln protein: MAGRGFQGMNYSYCLAAVLLVIISLTTMAMAKFVVEKNSLKVLSPDNIKGTYDSAIGNFGIPQYGGTMVGTAVYPKENQNGCKTFDTFNIEFKSKPGGLPNVVLVNRGDCYFALKVWNAQNAGAAAVLVSDDREEPLITMDSPEEDSSAAEYLQNITIPSALIEKEFGDKLKGAINKGDMVNINLDWRESVPHPDDRVEYELWTNSNDECGSKCDTQLEFIKNFKGAAQILEKSGYTQFTPHYITWYCPQAFTVSKQCKAQCINHGRYCAPDPEQDFSRGYDGKDVVLENLRQLCIFKVANESNRPWAWWDYVTDFHIRCPMKEKKYTKECAENVIQSLGLDNKKIETCMGDPNADAENPILKQEQDAQVGHGSRGDVTILPTLIINNRQYRGKLDKGPVLKAICAGFQETTEPTVCLSDDMETNECLKNNGGCWQDKKSNITACKDTFRGRVCECPLVDGVQFKGDGYSNCEGKKAEEGKVGWAAVWVVLLGLAAAAAGAYVVYKYRLRSYMDSEIRAIMAQYMPLDSQGEIPNHVNNETL*

Protein Length: 544

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTGCATTCCAATATTTGCTGCTTGGGATTGAACCGGCCAGGGATGATCCAAACCCTCACTCTACAAAACCTAGGACGATCCAGAGCTCTGGATATTGTGTCTGGTTCTGCGCTAAATCGGCTTAATTCGATCCACGGAAATGCTTTGCGACTAACGCAGCATGTCGAAGCGAACACATTTATGTCCGATTCGCAGAAAACAGTGTACCATTTATTCCAAGTTTGCTAACAACTGCTGAATCCCGAAAACCGGTCAAGCAATTGTCGGTTTTAATTGGATCTGCAATCTGCTTTCTTAAATTGGCGGTCTTAGTGCAGAATGCATATTTGTGGCCTTTTGAGAACTTTTCTTTTGCTGTATAGGGTTTGCTTATAAATAATCTGCTTGGCGATTTGCGGGGATTAATCTTCTTAGCCGTGAATTAAAACGTTTCACTGTTAATTTCTTCTCCAAAG_-_ATGGCGGGAAGAGGGTTTCAGGGTATGAATTACAGTTACTGTCTGGCCGCGGTGTTATTGGTGATAATCTCTTTGACGACCATGGCTATGGCGAAATTTGTGGTGGAGAAGAACAGCTTGAAAGTTCTCTCTCCGGATAATATTAAGGGCACTTATGACAGTGCTATAGGCAATTTTGGGATCCCTCAGTACGGTGGAACCATGGTCGGTACGGCTGTTTATCCGAAGGAGAATCAGAATGGCTGTAAGACTTTCGATACTTTTAATATTGAGTTTAAATCGAAGCCGGGAGGGCTTCCTAATGTCGTGCTGGTTAACCGTGGAGATTGTTATTTTGCTTTGAAAGTTTGGAATGCTCAGAATGCTGGTGCAGCAGCTGTTCTTGTTTCTGATGATAGAGAAGAGCCATTGATAACTATGGATTCTCCTGAAGAAGATAGTTCGGCAGCGGAATATCTGCAGAATATCACCATTCCTTCTGCCCTTATTGAGAAAGAGTTTGGAGACAAGCTGAAGGGGGCTATCAACAAGGGTGACATGGTTAATATTAATTTAGACTGGAGGGAGTCTGTTCCTCATCCAGATGATCGTGTGGAGTATGAGTTGTGGACAAATAGCAATGATGAATGTGGGTCAAAGTGTGATACACAACTGGAATTTATAAAGAATTTTAAAGGAGCTGCCCAAATTCTTGAGAAAAGTGGTTACACACAATTCACTCCTCACTATATCACCTGGTACTGTCCCCAAGCATTTACCGTAAGTAAGCAATGCAAGGCTCAATGCATCAATCATGGAAGATACTGTGCACCTGATCCAGAACAGGATTTCAGTCGGGGTTATGATGGTAAAGATGTTGTACTTGAAAATCTGAGGCAATTGTGTATCTTCAAAGTTGCAAATGAAAGTAATAGACCCTGGGCATGGTGGGATTATGTCACTGATTTCCATATTAGATGTCCAATGAAGGAGAAAAAGTATACCAAGGAATGTGCAGAAAATGTGATCCAGTCTCTAGGTTTGGATAACAAAAAGATAGAAACATGCATGGGGGATCCAAATGCAGATGCAGAGAACCCAATTTTGAAACAAGAGCAAGATGCACAGGTTGGACATGGATCACGAGGTGATGTCACCATATTGCCCACTCTCATTATTAACAACAGACAATATAGAGGAAAACTGGACAAAGGGCCTGTGTTGAAGGCAATTTGTGCAGGCTTTCAGGAAACTACTGAGCCAACTGTATGCTTGAGTGATGATATGGAAACAAATGAATGTTTGAAAAATAATGGTGGTTGCTGGCAAGACAAGAAGTCAAATATAACTGCCTGCAAGGATACTTTTCGGGGAAGGGTTTGTGAGTGTCCCTTGGTAGACGGTGTTCAATTTAAGGGAGATGGTTATTCTAACTGTGAAGGTAAAAAGGCTGAAGAGGGAAAGGTTGGCTGGGCTGCTGTATGGGTTGTATTATTAGGTTTGGCCGCTGCTGCTGCTGGAGCTTATGTAGTTTACAAATACAGACTAAGATCATACATGGACTCTGAGATCAGGGCCATCATGGCACAATACATGCCTCTCGACAGCCAAGGAGAGATTCCTAACCATGTTAATAATGAGACGTTATAA___AATTCTAGAGAGTCGAGACTCCTGTGTAATGACTCTTCATGTTCGTGGCTATTGTGAGTACATATTAGAAGTTCTCTGCTTGATCTTACAAAAGCTGTGCAAATTATAATGTTCTTACAATTCAAAGACATCCTTTGGGAAGCTCCTCATTCATGAACATTGTACCCACATTCAGACTGTATATTAAGAGTTGGGAAGAGGATTAGATATTAGCAATCCAGTTTCAGGTCTTTACTTGGTTGATATGGTTTCTTGACAGACAGATGACTTGGTTATCTTGTTGTAACTCCAGTTAGTCTTCAAAGTTCCAACATTACAGTTTTGCAGTATCTGAAAATGATTTATTAAAGGAGGTTGCTGATAGAGCAGAGCTTTTATTGAGTAGAAAGTCTACTTGAAGGCTAACACTGCCATGTTTCTGAGTATAGTTTAGTCACTTCCTTTAACTACATAGTCTTAAAGATGGTCTCTTTATCTAGAAATCTTTAAAAATTATTGGAGAATAAACATGTTGAAGGTTTACATCTTACACGAGGTCAATCTAGCATCTCCATCTTTTGCATGTTTTTGTGCAGTTCTTTGTTGTTATGATTATTTCAATTATCTGAAATTTCGTATTGTACCATACATT

Fln Alignment:

AllPineV4_rep_c112141___MAGRGFQGMNYSYCLAAVLLVIISLTTMAMAKFVVEKNSLKVLSPDNIKGTYDSAIGNFGIPQYGGTMVGTAVYPKENQNGCKTFDTFNIEFKSKPGGLPNVVLVNRGDCYFALKVWNAQNAGAAAVLVSDDREEPLITMDSPEEDSSAAEYLQNITIPSALIEKEFGDKLKGAINKGDMVNINLDWRESVPHPDDRVEYELWTNSNDECGSKCDTQLEFIKNFKGAAQILEKSGYTQFTPHYITWYCPQAFTVSKQCKAQCINHGRYCAPDPEQDFSRGYDGKDVVLENLRQLCIFKVANESNRPWAWWDYVTDFHIRCPMKEKKYTKECAENVIQSLGLDNKKIETCMGDPNADAENPILKQEQDAQVGHGSRGDVTILPTLIINNRQYRGKLDKGPVLKAICAGFQETTEPTVCLSDDMETNECLKNNGGCWQDKKSNITACKDTFRGRVCECPLVDGVQFKGDGYSNCE
B8LPV3_______________MAGRGFQGMNYSYGLVAVLLVIIYLKTMAMAKFVVEKNSLRVLSPENIKGNYDSAIGNFGIPQYGGTMVGTAVYPKENQNGCKTFDTFEISFKSKPGGLPNVVLVNRGDCYFALKVWNAQNAGAAAVLVSDDRDEPLITMDSPEEDSSAAEYLQNITIPSALIEKAFGDKLKVAINKGDMVNINLDWRESVPHPDDRVEYELWTNSNDECGAKCDTQLEFAKNFKGAAQILEKSGYTQFTPHYITWYCPQAFTVSKQCKAQCINHGRYCAPDPEQDFSRGYDGKDVVLENLRQLCIFKVANESNRAWVWWDYVTDFHIRCPMKEKKYNKECAENVIQSLGLDAKKIDKCMGDPNADAENPVLKQEQDAQVGHGSRGDVTILPTLIINNRQYRGKLDKGAVLKAVCAGFQETTEPTVCLSEDMETNECLKNNGGCWQDKKANITACKDTFRGRVCECPLVEGVQFKGDGYSNCE

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
1966		90	10		0.90298
1967	10			90	0.90298
1968		90	10		0.90298
1969		88		11	0.991528
1972	11		88		0.991528
1974		11		88	0.991528
1975		11		88	0.991528
1979		88	11		0.991528
1980	88		11		0.991528
1981			88	11	0.991528
1988	87		12		0.992554
2061		20		80	0.995493
2064	25			75	0.996473
2553		33		66	1.0
2554	33		66		1.0
2555	33			66	1.0
2556			33	66	1.0
2557			33	66	1.0
2561	33		66		1.0
2653	85		14		0.993533
2664	14	14			0.984337
2666	14	85			0.993533
2734	80	20			0.995505
2735	75			25	0.996473
2741	25			25	0.995533
2742	75			25	0.996473
2743	75		25		0.996473
2745		25		75	0.996473
2748			25	75	0.996473
2749		25	75		0.996473
2750		25	75		0.996473
2753			25	75	0.996473
2755		80		20	0.995498
2756	80	20			0.995498
2760		20		80	0.995498

SSRs (tot: 1)

Start position	End position	Sequence	Length
763	775	CAG CAG CAG CAG C	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain