SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene62570

Length: 859 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene62570 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	salt-induced AAA-Type ATPase [Mesembryanthemum crystallinum]	-	-	78%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	62%
Blast2go	vacuolar sorting protein 4b	-	-	92%

Source	InterPros	Term	Type	Identity
Sma3	Kinesin, motor domain	IPR001752	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	ATPase, AAA-type, core	IPR003959	-	-
Sma3	ATPase, AAA-type, conserved site	IPR003960	-	-
Sma3	MIT	IPR007330	-	-
Sma3	Vps4 oligomerisation, C-terminal	IPR015415	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9P2N1

Fln msg: Overlapping hits, possible frame ERROR between 178 and 160, and possible frame ERROR between 219 and 222, your sequence is shorter than subject: 241 - 439

Fln protein: TGKSYLAKAVATEADSNVFQMARDSAPSIIFIDEIDSLCGTRGESNESEASRxxxxxxxxQMQGVGNNDDKVxxLAATNTPYALDQAIRRRFDKRIYIPLPEVKARQHMFKVHLGDTPHNLTESDFEELACRTEGFSGSDIAVCVKDVLFEPVRKTQDAMFFRKVSTEEGEMWMPCGPRQAGAVQTTMQELAAEGLAAKILPPPIAKADFDKVIARQRPTVSKSDLEVHERFTKEFGEDG*

Protein Length: 242

Fln nts: AACTGGAAAGTCATACTTGGCAAAAGCTGTTGCGACCGAAGCAGATTCTAACGTTTTTCAGATGGCCCGTGATTCTGCACCTTCTATTATATTCATTGATGAAATAGATTCTTTATGTGGCACACGTGGAGAAAGTAATGAGAGTGAGGCTTCTCGAxxxxxxxxxxxxxxxxxxxxxxxxCAGATGCAGGGTGTAGGAAACAATGATGATAAAGTCGxxxACCTGGCTGCCACCAACACTCCATATGCATTGGATCAGGCAATTCGGCGTCGGTTTGACAAAAGAATTTATATCCCACTACCAGAGGTCAAGGCAAGGCAGCATATGTTCAAGGTCCATTTAGGTGACACACCCCACAACTTAACTGAGAGCGATTTTGAGGAACTGGCATGCAGGACAGAGGGCTTTTCTGGATCTGATATAGCTGTCTGTGTTAAAGATGTACTCTTTGAACCTGTTCGTAAAACTCAAGATGCCATGTTCTTCAGAAAGGTTTCCACTGAGGAAGGGGAGATGTGGATGCCATGTGGTCCTAGGCAAGCTGGTGCTGTGCAGACAACCATGCAAGAACTTGCAGCAGAAGGCTTAGCGGCCAAGATTCTGCCACCACCAATTGCAAAAGCAGATTTTGATAAAGTTATCGCAAGGCAAAGGCCAACTGTTAGCAAAAGCGACCTTGAGGTGCATGAGAGATTCACGAAGGAGTTTGGAGAAGACGGCTGACAAATTTCAGTCTAATTGTATATGAATGCAGGAAAAGGGGCTCCAGCGTTTTGGGTAAAAATGGTTTCATTGCAACGATGTTATTGAATCTGGTGTTCTACTTCTTACGAGGAAAATCTTGCCCATTATGGGAAAAA

Fln Alignment:

AllPineV4_rep_c75642___TGKSYLAKAVATEADS-----------------------NVFQMARDSAPSIIFIDEIDSLCGTRGESNESEASRRxxxxxxVQMQGVGNNDDKVVxLAATNTPYALDQAIRRRFDKRIYIPLPEVKARQHMFKVHLGDTPHNLTESDFEELACRTEGFSGSDIAVCVKDVLFEPVRKTQDAMFFRKVSTEEGEMWMPCGPRQAGAVQTTMQELAAEGXXXXXXXXXXXXADFDKVIARQRPTVSKSDLEVHERFTKEFGEDG
A9P2N1_______________TGKSYLAKAVATEADSTFYSISSSDLVSKWMGESEKLVSNLFQMARDCAPSIIFIDEIDSLCGQRGEGNESEASRRxxxxxxVQMQGVGNNDQKVLxLAATNTPYALDQAIRRRFDKRIYIPLPDLKARQHMFKVHLGDTPNNLTEGDFEDLARRTDGFSGSDISVCVKDVLFEPVRKTQDAMFFSKVSTKDGEMWMPCGPRQPGAVQTTMQELAVKGLASQILPPPISKADFDKVLARQRPTVSKHDLEVQERFTKEFGEEG

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
279		33		66	0.997452
285		33		66	0.997453
583		20		80	0.995493
591		80		20	0.995493
597	20	80			0.995493
598		80	20		0.995493
599			80	20	0.995493
600	80		20		0.995493
602		20		80	0.995493
603	20			80	0.995493
608	20			80	0.995493
610	80		20		0.995493
615	80		20		0.995493
617		20		80	0.995493
636	50		50		1.0
645	50		50		1.0
646	50			50	1.0
649	50	50			1.0
654			50	50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain