SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene62332

Length: 1410 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene62332 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Aspartic proteinase nepenthesin-2, putative n=1 Tax=Ricinus communis RepID=B9SD00_RICCO	-	-	37%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	51%
Blast2go	aspartic proteinase nepenthesin-2-like	-	-	51%

Source	GOs	Term	Type	e value	Identity
Blast2go	cell wall	GO:0005618	Cellular Component	0.0	51%

Source	InterPros	Term	Type	e value	Identity
Sma3	Peptidase A1	IPR001461	-	0.0	-
Sma3	Peptidase aspartic, catalytic	IPR009007	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AC09

Fln msg: your sequence is longer than subject: 429 - 370

Fln protein: MVASKLKLMFFIFLSLQLSATLTQPKEHEMDGVSMTLHMKMRMKIKSSKKINVEEPLYYQSEDIEYYIKLSVGTPAQDLLLVMDTGSDVVWAPCTTNYTCNYNCGGYGTVFLPRKSSSHVPIKCADPKCRDLCDNEGCVESFQNCSGICPHFNLRYGSGNATGRLLSDTLTLALEEGGRREKKRFAIGCSVVSSGFSGIAGFGRGGLSMPSQLAPLLGDKFAYCLDHVSNSSKIVLGNKAVPRNIPFTWTPLFINPVNPSVFTFYYLGLQAVSIGGKRLTLPSNLLSFDSHGNGGTAIDSGSSYTYFPDSIYKQIAGEFASRIGYKKAPGFEAVTGNGLCYNVSGLENIQWPKFVFHFKCGSDMVLPDNNSFTAAASDTFCLAMGNSGPLVNGPAVLVGNFQQNNLYILYDREKNRLGFTQQSCALLG*

Protein Length: 430

Fln nts: ACATGGGACTCATGGCCAATGGGAATCCCATTCTTCCCCGATTAGGTTTCCCTAAGAAGTCGTACT_-_ATGGTGGCCTCCAAGTTGAAGCTGATGTTCTTCATTTTCCTCTCACTGCAACTCTCTGCAACATTGACTCAACCCAAAGAACATGAAATGGATGGAGTGTCCATGACCCTCCACATGAAGATGAGGATGAAGATCAAGTCTTCAAAGAAGATCAACGTGGAGGAGCCTCTGTACTATCAAAGCGAGGATATCGAGTATTACATCAAATTGTCGGTGGGTACTCCTGCACAGGATCTATTGCTCGTCATGGACACGGGTAGTGATGTGGTGTGGGCACCCTGCACCACAAATTATACATGTAATTACAACTGCGGTGGATACGGCACGGTTTTTCTTCCAAGAAAGTCATCCTCTCATGTTCCTATAAAGTGCGCAGATCCGAAGTGCAGAGACCTGTGCGATAATGAGGGCTGTGTGGAAAGCTTCCAGAATTGCTCCGGGATTTGTCCACATTTCAACCTTCGCTATGGCTCGGGAAACGCGACGGGGCGTCTACTGAGTGACACCTTGACTTTGGCACTGGAAGAGGGAGGAAGACGAGAAAAAAAGCGGTTCGCAATAGGATGTTCAGTGGTTTCATCCGGGTTTTCCGGTATTGCAGGATTTGGCAGAGGAGGACTGTCTATGCCTTCCCAGCTTGCTCCTCTTCTTGGTGATAAATTTGCGTATTGCCTTGACCATGTAAGCAACAGCAGCAAGATAGTGCTTGGTAACAAGGCGGTTCCCAGGAATATCCCTTTCACTTGGACTCCCCTTTTCATCAACCCAGTAAACCCTTCGGTTTTCACTTTCTATTACCTTGGCCTCCAAGCAGTGAGTATTGGTGGAAAGCGCTTGACGCTGCCGTCCAATTTATTGTCATTTGACAGCCATGGCAATGGCGGAACCGCAATAGATTCGGGTTCTTCTTACACTTACTTTCCTGATTCAATCTATAAGCAGATTGCAGGTGAGTTTGCTTCTCGAATTGGATATAAAAAAGCTCCTGGATTTGAAGCTGTTACTGGTAATGGGTTGTGTTACAATGTGTCTGGTTTAGAAAATATTCAGTGGCCTAAATTTGTATTCCATTTCAAGTGTGGCTCAGACATGGTACTACCTGATAATAATAGTTTCACGGCTGCCGCTTCTGATACATTCTGCTTGGCAATGGGGAATAGTGGCCCTCTTGTTAACGGACCTGCCGTTCTCGTAGGAAACTTTCAGCAGAACAATTTATATATATTGTATGACAGAGAGAAGAATCGTTTGGGCTTCACTCAGCAGTCGTGTGCATTATTGGGATGA___GTCATTGATGCAGCGCTCTACATATTTTCCATAGTGTATGATTTCTTTGAACATATG

Fln Alignment:

AllPineV4_rep_c74004___MDTGSDVVWAPCTTNYTCNYNC---GGYGTVFLPRKSSSHVPIKCADPKCRDLCDN------EGCVESFQNCSGICPHFNLRYGSGNATGRLLSDTLTLALEEG-GRREKKRFAIGCXXXXXXXXXXXXXXXXXXS-MPSQLAPLLG-DKFAYCL-----DHVSNSSKIVLGNKAVPRNIPFTWTPLFIN---PVNPSVFTFYYLGLQAVSIGGKRLT-LPSNLLSFDSHGNGGTAIDSGSSYTYFPDSIYKQIAGEFASRIGYKKAPGFEAVTGNGLCYNVSGLENIQWPKFVFHFKCGSDMVLPDNNSFTAAAS-DTFCLAMGNSGPLV---NGPAVLVGNFQQNNLYILYDREKNRLGFTQQSC
D5AC09_______________MDTGSDLVWVPCTRNYSC-INCPEDSASNGVFLPRMSSSLHLVTCADSNCKTLYGNNTELLCQSCAGSLKNCSETCPPYGIQYGRGSTAGLLLTETLNLPLENGEGARAITHFAVGCSIVSSQQPSGIAGFGRGALSMPSQLGEHIGKDRFAYCLQSHRFDEENKKSLMVLGDKALPNNIPLNYTPFLTNSRAPPSSQYGVYYYIGLRGVSIGGKRLKQLPSKLLRFDTKGNGGTIIDSGTTFTVFSDEIFKHIAAGFASQIGYRRAGEVEDKTGMGLCYDVTGLENIVLPEFAFHFKGGSDMVLPVANYFSYFSSFDSICLTMISSRGLLEVDSGPAVILGNDQQQDFYLLYDREKNRLGFTQQTC

SNPs (tot: 42)

Position	A %	C %	G %	T %	Probability
796		50	50		1.0
797		50		50	1.0
803	40			60	1.0
820		50	50		1.0
822		60		40	1.0
826		50		50	1.0
827	50		50		1.0
831		50	50		1.0
832		50		50	1.0
865		50		50	1.0
880	80		20		0.995493
883		50	50		1.0
884		50		50	1.0
900		20	80		0.995493
910		40	60		1.0
916	50	50			1.0
1030		14		85	0.993533
1041		14		85	0.993533
1051		14		85	0.993533
1090	57		42		1.0
1109	60	40			1.0
1113		50	50		1.0
1122		60	40		1.0
1132		66		33	1.0
1163		60	40		1.0
1167		83	16		0.994512
1194	28	14		57	1.0
1207	50			50	1.0
1208	85		14		0.993533
1215	50	50			1.0
1243		28		71	1.0
1259		57	28	14	1.0
1281	80		20		0.995493
1283	40			60	1.0
1290		20		80	0.995493
1308	20	20	60		0.99255
1357	50	50			1.0
1360		75		25	0.996473
1388			66	33	0.997471
1394		66	33		0.997476
1396	66	33			0.997452
1397	33		33	33	0.997752

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain