SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene60959

Length: 1266 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene60959 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Tubulin alpha-6 chain n=85 Tax=Eukaryota RepID=TBA6_ARATH	-	-	98%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	98%
Blast2go	alpha-tubulin	-	-	98%

Source	GOs	Term	Type	e value	Identity
Blast2go	cellular protein metabolic process	GO:0044267	Biological Process	0.0	98%
Blast2go	protein binding	GO:0005515	Molecular Function	0.0	98%

Source	InterPros	Term	Type	Identity
Sma3	Tubulin	IPR000217	-	-
Sma3	Alpha tubulin	IPR002452	-	-
Sma3	Tubulin/FtsZ, GTPase domain	IPR003008	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3	Tubulin, conserved site	IPR017975	-	-
Sma3	Tubulin/FtsZ, 2-layer sandwich domain	IPR018316	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: C0PQU1

Fln msg: Separated hits, possible frame ERROR between 489 and 495, your sequence is shorter than subject: 320 - 450. Was predicted as Complete, but is very much shorter than de subject

Fln protein: MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNxxxVISAEKAYHEQLSVAEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESAEGEEGDEGDEY*

Protein Length: 321

Fln nts: CGTCGTACCTTGGCTGTCACTCGTCGTGACCTTGCTTTTTAGGGTTTTTTTCCTTCGTCACCAGCGAAA_-_ATGAGAGAGTGCATCTCGATCCACATTGGACAGGCCGGTATCCAGGTTGGAAATGCTTGCTGGGAACTTTACTGCCTCGAGCACGGCATTCAGCCTGATGGCCAGATGCCCAGCGACAAAACTGTCGGTGGCGGAGACGATGCATTCAACACTTTCTTCAGCGAAACTGGAGCCGGAAAGCATGTTCCCCGCGCTGTCTTTGTAGATCTGGAGCCGACTGTCATTGACGAGGTACGGACTGGTACTTACCGTCAGCTTTTCCATCCTGAACAGCTTATCAGCGGCAAGGAAGATGCGGCCAACAACTTTGCCCGTGGTCATTACACCATTGGCAAGGAAATTGTGGACCTCTGCCTGGACCGTATTCGGAAGCTGGCTGACAACTGCACGGGTCTTCAAGGATTCCTCGTCTTCAACGCxxxxxxAGTGATCTCTGCAGAAAAGGCCTACCATGAGCAGCTTTCTGTGGCAGAAATTACTAACAGTGCATTTGAGCCGTCCTCCATGATGGCCAAGTGCGATCCTCGCCACGGAAAATACATGGCCTGTTGTCTTATGTACCGTGGAGATGTCGTGCCCAAGGATGTCAATGCCGCTGTCGCAACGATTAAGACAAAGAGAACCATCCAGTTTGTTGATTGGTGTCCCACAGGATTTAAATGCGGAATCAACTACCAGCCTCCCACCGTTGTCCCCGGAGGCGATCTCGCCAAGGTTCAGAGAGCCGTGTGCATGATTTCCAACTCGACCAGTGTTGCAGAGGTGTTCTCGAGAATCGATCATAAGTTCGATCTCATGTATGCCAAGCGAGCATTTGTTCATTGGTATGTGGGCGAGGGTATGGAAGAGGGCGAGTTTTCTGAAGCGCGTGAGGATCTTGCTGCCCTCGAGAAGGACTATGAGGAGGTTGGCGCTGAGTCTGCAGAGGGAGAGGAAGGTGATGAGGGAGACGAGTACTAA___TCACCCCTTCAGATAGCAATGCTGATTGTTGGTACACGAGGTGATGTACAACCATTCGTAGCCATTGGTAAACATTTGCAGGAGTATGGGCACCGTGTGAGATTAGCAACTCATTCAAATTTTGAGGAATTTGTATTGACGGCTGGTCTTGAGTTCTATCCACTTGGGGGAGATCCAAAAGTTCTTGCTGAATACATGGTTAAGAACAAAGGGTTTTTGCCATCTGGGCCATCAGAGA

Fln Alignment:

AllPineV4_rep_c66441___MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAxxVISAEKAYHEQLSVAEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD
C0PQU1_______________MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHAxxVISAEKAYHEQLSVAEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
913		10		90	0.90298
1150		88	11		0.999952
1151	11	88			0.999952
1152		88	11		0.999952
1153	88			11	0.999952
1155	11	88			0.999952
1156	88	11			0.999952
1162	5		11	76	0.999894
1163			17	82	1.0
1167	87		12		0.999964
1170		87	12		0.999964
1235	90	10			0.903003
1237	70	10			0.999998
1239	90			10	0.903076
1243		66	22		0.999999
1244	22	66			0.999999
1245		66	33		1.0
1248	66		33		1.0
1251	77		22		0.999999
1252	66	33			1.0
1253	77	22			0.999999
1254	66		33		1.0
1255	33	33	33		1.0
1257	33	55		11	1.0
1258	55		44		1.0
1261	33		55	11	1.0
1262	22			55	0.999999
1263	33	55	11		1.0
1265	22	77			0.999999
1266	11	22	55	11	0.999999
1267	55		22	22	0.999999
1268		77		22	0.999999
1269		11	88		0.923584
1274		33	66		0.997461
1279	33	66			0.997461

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain