SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene60253

Length: 1503 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene60253 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cathepsin B-like cysteine protease n=2 Tax=Oryza sativa RepID=Q5D214_ORYSJ	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	cathepsin b-like	-	-	81%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Peptidase C1A, papain	IPR013128	-	-
Sma3	Peptidase C1A, papain C-terminal	IPR000668	-	-
Sma3	Peptidase C1A, cathepsin B	IPR015643	-	-
Sma3	Peptidase C1A, propeptide	IPR012599	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NKL4

Fln msg:

Fln protein: MAWRFLFCSMVMVAMVATIQASLPESFLTQSQDGILKEPMVEEINRHPNAGWKAGMNPRFSNHTVGQFKHLLGVLPTPRKLLESVPLRTYPKGLDLPKEFDARKAWPQCTSVRTILDQGHCGSCWAFGAVEALSDRFCIHYEVNVTLSENDLVACCGFRCGEGCEGGYPLSAWQYFISTGVVTAECDPYFDEAGCQHPGCEPLYPTPQCVKQCKDENQDWGNSKRFSATAYRINSKPYDIMAEVYTNGPVEVDFLVYEDFAHYKSGVYKYIKGDYLGGHAVKLIGWGTENGTDYWLVANSWNTAWGEDGYFKIVRGSNECNIEEDVVAGMPSTKNLVSVPAAGTEGHASS*

Protein Length: 352

Fln nts: CGTCGTGACCTTGGCTGTCATGGGGATCCAACGAGAGGTAGTAGTACTGCAAAAGTGAGCGGCT_-_ATGGCATGGAGATTCCTTTTCTGTTCAATGGTCATGGTGGCCATGGTTGCTACAATTCAGGCATCTCTGCCGGAGTCCTTTCTCACACAGAGCCAAGATGGAATCCTCAAGGAACCCATGGTAGAAGAAATTAACAGGCATCCAAATGCTGGATGGAAGGCTGGCATGAATCCTCGGTTTTCCAATCACACAGTAGGACAATTTAAGCATCTCCTAGGAGTACTTCCTACCCCCAGGAAATTATTAGAAAGTGTTCCTCTCAGAACTTATCCGAAAGGTTTGGACTTGCCAAAGGAATTCGATGCACGAAAAGCTTGGCCGCAGTGTACTTCTGTTCGAACCATCCTAGATCAGGGGCACTGTGGTTCTTGCTGGGCATTTGGTGCAGTGGAAGCACTTTCTGATAGGTTTTGCATTCACTATGAAGTGAACGTTACTCTCTCCGAAAATGACCTGGTGGCATGTTGTGGATTCCGGTGTGGGGAAGGCTGTGAAGGTGGTTATCCATTATCCGCATGGCAATACTTCATCAGTACTGGTGTTGTAACTGCCGAGTGTGACCCCTACTTTGATGAAGCCGGTTGCCAACATCCAGGCTGCGAGCCTTTATATCCAACTCCCCAGTGTGTGAAGCAATGCAAAGATGAAAACCAAGACTGGGGCAATTCCAAACGCTTTAGCGCAACTGCTTACAGAATTAACTCCAAGCCATATGATATCATGGCGGAGGTCTATACAAATGGCCCTGTGGAGGTTGATTTCCTTGTTTACGAGGACTTCGCTCATTACAAGTCCGGTGTTTACAAGTACATCAAGGGTGACTACCTGGGAGGACATGCTGTGAAGCTGATTGGTTGGGGAACTGAGAATGGAACTGATTATTGGCTGGTGGCAAACTCATGGAATACAGCATGGGGTGAGGATGGTTATTTTAAAATTGTTAGGGGTTCTAATGAATGTAACATAGAAGAAGATGTGGTCGCAGGGATGCCTTCAACGAAGAATTTAGTAAGTGTTCCTGCAGCTGGCACTGAAGGCCATGCATCTTCGTAG___TCTTCCATTTTACAGATCTGCAACTGCAATAAGCTAGTAATTTTATTTGTACAAAAGACGCTGTAAATATATTTGGTAGTGCTGATATCAAGGTACTTGTGGTCTTTTGCTTGTTAAAGGAATCTGATGAGCAAGATAAAACTAAAGAACGTAAATTATCTGTAACATTTATATTTGCTTGGATGTTAGTTTTCATACTGGGAACAAGTTGTGTTTGCTATTTGGGCTTTGCTAGGACTACAATGGTTGGGTCTTGTATTATAGTCTTCATTTCTTTTGGACAAGGTGGCAGTTATGTTTATTTGCTAACACAAAATGCTGCACCCTCAAATGGTTCTCTGAATAAGAAAAAAGAGTGATATATTTTATGTACAAGAAAAAAATCT

Fln Alignment:

AllPineV4_rep_c62136___MAWRFLFCSMVMVAMVATIQASLPESFLTQSQDGILKEPMVEEINRHPNAGWKAGMNPRFSNHTVGQFKHLLGVLPTPRKLLESVPLRTYPKGLDLPKEFDARKAWPQCTSVRTILDQGHCGSCWAFGAVEALSDRFCIHYEVNVTLSENDLVAXXXXXXXXXXXXGYPLSAWQYFISTGVVTAECDPYFDEAGCQHPGCEPLYPTPQCVKQCKDENQDWGNSKRFSATAYRINSKPYDIMAEVYTNGPVEVDFLVYEDFAHYKSGVYKYIKGDYLGGHAVKLIGWGTENGTDYWLVANSWNTAWGEDGYFKIVRGSNECNIEEDVVAGMPSTKNLVSVPAAGTEGHASS
A9NKL4_______________MASRLLFCLMVLVAMAATPQASLVESFPAQSQDRILKEPIVEEINRHPKAGWKAGMNSRFSNHTVGQFKRLLGVLPTPRNLLENVPVRTYPKGLNLPKQFDARKAWPQCTSVRTILDQGHCGSCWAFGAVEALSDRFCIHYKVNVTLSENDLVACCGFRCGDGCDGGYPLSAWQYFISTGVVTAECDPYFDEAGCQHPGCEPLYPTPQCVKQCKDENQNWGNSKRFSATAYRITSKPYDIMAEVYTKGPVEVDFLVYEDFAHYKSGVYKYITGDFLGGHAVKLIGWGTENGTDYWLVANSWNTAWGEDGYFKIARGSNECSIEEDVVAGMPSTKNLVMDPAAGTKGHASS

SNPs (tot: 38)

Position	A %	C %	G %	T %	Probability
471	2		97		0.956062
535	97			2	0.969538
538		95	4		0.99997
540		97	2		0.971188
544		97	2		0.969538
548	2			97	0.969538
552	2			97	0.966038
553		2	97		0.966038
554			2	97	0.966038
556		2		97	0.966038
557			2	97	0.966038
639	96			3	0.984346
645	3			96	0.98536
648		96	3		0.986325
651	3			96	0.98536
652	3			96	0.986325
654	96		3		0.986325
657		3		96	0.987242
660	3			95	0.918242
661		3		96	0.987242
817		4		95	0.99692
1186	2		97		0.914425
1190			97	2	0.927345
1199	2			97	0.960269
1203	2		97		0.964186
1204		2	97		0.964186
1207	97			2	0.966038
1345		1		93	0.999965
1377	2			97	0.971188
1379			2	97	0.972772
1473		3	96		0.991164
1516		32	67		1.0
1517		32	67		1.0
1520		66		5	1.0
1521		72		27	1.0
1522		72	27		1.0
1523	33	66			1.0
1524	66		27	5	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain