SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene60209

Length: 3012 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene60209
CGTCGTGACCTTGGCTGTCACTCATCATCTTCGTCGTGACCTTGGCTGTCACTCAATTCGCAAACTGTGCCCTTTACTAATCGATGTACAATTTTTGACATCCGCAATTTAAAAGATTTTCAGATCCGCTTTAAGGCCGCAAAGAGTGACGATAACTCAGGAAAGAAGCACAGAATCACTAGGCCGACCGGGACGTTCATTTTGTAGGTTGTTGGTGAACTGACGGAACTCCGATCCTAAAAAACAAACCGCAGAATGGCTGAACCAGACTCGTGGGAGGAGATGGATTCTCAGCCTTCGCATTCTGCAGCTGCCGCATCATCGATGATGTCAAAATTGAACGCAAATGCCCCGTCGTTTACATTTAATGTCAAAGCCCCTGCTTTTTCACCGGTTCCGAACGCGTATTTTCCCGCACAACCTAGTACACAACAGGCCTTTTTTCCGACCAATCCTGTGAACTCCACCAATCCTCAATTTGTAAACCCTACAAGCCCCATACAACCTATCAGCCCTACAAACCCCTCTGCAGGGTTGCCCCCACAGAACGCACCGGGGGCCGTTTCACCATACGTCCAACCCTCATATCCTCCTGCGGTCGACCATCAAGTGGCGGCTAGAAATGACGTTGCCTCTAAAATTGAAACCCTGCGGCATTCTGAGCGGGAGCGGTATGAAACGAGGCCGGACTTAACCGATAAGGATGTTGAGATGGAAGTGGATGGTGGATCTGCCCAAGTCGAAGCAAAAGAAACGGCTGCCGCCAACGGCGAGAGTAACGGTGCGTTGAGAGGGGACGAGGAGCAGGTAGTATCTAATTTGAACTCATTGAAATTAAAGGACACAGAACAGAATAAAGAAGTTGACATTAAGCATGAAGAAATGGCTCAAGAACATTTTGAGGATTCCCAACAATTGGAAGAGGAAGAGGAGATTGAAGAGAAGCCACACATGAATGTTGTCTTTATTGGTCATGTTGATGCTGGTAAATCAACAACTGGTGGGCAAATATTATATCTATCTAAAATGGTGGATGAGCGTACAATTCAAAAATATGAAAAAGAAGCAAAGGAAAAGAACCGAGAAAGTTGGTATATGGCATATATCATGGACACAAATGAGGAAGAACGAGTAAAGGGAAAGACAGTGGAGGTTGGTAGAGCTCATTTTGAAACTGAGAAGACAAGATTTACAATCTTGGATGCTCCAGGGCACAAAAGCTACGTTCCAAACATGATTAGTGGTGCATCACAGGCTGATGTAGGAGTTTTGGTGATATCGGCTCGAAAGGGTGAGTTTGAGACTGGATTCGAAAGAGGTGGTCAGACAAGAGAGCATGCTATGTTGGCAAAAACATTAGGAATTTCTAAACTATTTGTGGTGGTCAATAAAATGGATGATCCTTCAGTTGAGTGGGCCAAGGAGAGGTTTGATGAAATTGAAAAGAAGATAAGCCCCTTCTTGAAGTCTTGTGGCTACAATGTAAAGAAAGATGTGCTGTTTCTTCCAATATCAGCACTTCATGGTACTAACATCAAAGAGAAGATCCCAAAAAATGTGTGTGGTTGGTGGAATGGTCCAACCCTTTTTGAAGGGTTGGATTCAATCGAATTACCTCCAAGAGATCCTAAAGGTCCTTTCAGGATGCCATTAATAGATAAATTCAAGGATATGGGAACTGTGGTTATGGGAAAAATAGAGTCAGGCACTGTAAGGCAAGGAGACAGCTTAATTATAATGCCAAATAAGGTTCAAGTGAAAGTTGTAGCTGTTTATCGTGAAAGTGATGAAATAAAGTATGCAAGGCCTGGAGAAAATGTTAGGGTGAAATTATCTGGTGTTGAAGAAGATGACATCTCTACAGGCTTTGTTTTGTCTTCCATTTCAAATCCAATTCAAGCAGTGCTGGAGTTTGAGGCTCAGTTACAGATATTAGAACTACTGGAACATAAAGCAATATTTACTGCTGGTTATAAAGCTGTACTGCATATTCATTCTGTTGTCGAGGAATGTGAGATAATGGAGCTGTTAGAGCAGATGGATCCAAGGACGAAAAAGCCAATGAAAAAGAAATGTCTTTTTGTTAAGACTGGAGCAGTTGTGTTGTGTCGCATTCAGGTAAATGACACTATCTGTGTTGAGAAGTTCTCTGATTTTCCTCAACTTGGAAGGTTCACACTTCGGGATGAAGGTAAAACAGTCGCAATTGGAAAAGTTGTGAAGCTCCTAACTGCCACAGGACATGAATAACCGACGGAAATTGACTGAGACTATCCCTCGGCAATTATTCGATATGCACTAATAGAATGAGTGGGGCCTCGAGGGATTCTTTAATTCATCTTTCTGCACAGATTATGTGAGGCAACCCTTGAAATCGTTTCAATTTTCAGGTGCAAGGTGAACATGATGTCAGTAATCTTTATTTCTTGAACTGTCTTCAAGTGAAGAATGATGCAAGGGATGGATAAAGCGTTCATTGTGGTAGCAGTGAAGGTTTAGATATATATAGAATGATTGCTATTTTTGCAAGCCTCTATTCTGCCATGTCATGGGGGGCATTTAAACAAGAATACACAGATTTAACAGATTTAAGTGAACCTCAATTATAGGTCTGTTTGTATCTGGTCTATATTTTGTTCAATTTTATGTGGACGTAAGAGTTGTAATATTTTCAGCAATTTCATGTGCTCTAATGGCTTGAACATTGTTTGTTCAGCTTATATAAAGACACTGAATTTTCAACGATTTTACCGTTCTAATAGCTTGAGCATTATCTGTTCAGCTAATATTAAGGCACTGAATTATTTGTTTATCAGCGTTCTATGGTCAAGCCATTTCTCGTGATCTTGTGGCTTGGGCATTATATGTTCAGCTGACATTAGAGAGTTAATGGCTTTTAATCACCATTCATTTATTTCATGTTCTTGTTTTGAATATTTGGAACTATGATTTGCCCTGGTTGCTCAATGTATATAAACTTCTGGGGTAAACCCAAAAATTCTGTTGCCATGA

Ace file of the UniGene sp_v2.0_unigene60209 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	[J] COG5256 Translation elongation factor EF-1alpha (GTPase)	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	g1 to s phase transition protein	-	-	83%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	83%
Blast2go	vacuole	GO:0005773	Cellular Component	0.0	83%

Source	InterPros	Term	Type	Identity
Sma3	Protein synthesis factor, GTP-binding	IPR000795	-	-
Sma3	Translation elongation factor EFTu/EF1A, domain 2	IPR004161	-	-
Sma3	Translation elongation factor EFTu/EF1A, C-terminal	IPR004160	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNF8

Fln msg: your sequence is longer than subject: 665 - 466

Fln protein: MAEPDSWEEMDSQPSHSAAAASSMMSKLNANAPSFTFNVKAPAFSPVPNAYFPAQPSTQQAFFPTNPVNSTNPQFVNPTSPIQPISPTNPSAGLPPQNAPGAVSPYVQPSYPPAVDHQVAARNDVASKIETLRHSERERYETRPDLTDKDVEMEVDGGSAQVEAKETAAANGESNGALRGDEEQVVSNLNSLKLKDTEQNKEVDIKHEEMAQEHFEDSQQLEEEEEIEEKPHMNVVFIGHVDAGKSTTGGQILYLSKMVDERTIQKYEKEAKEKNRESWYMAYIMDTNEEERVKGKTVEVGRAHFETEKTRFTILDAPGHKSYVPNMISGASQADVGVLVISARKGEFETGFERGGQTREHAMLAKTLGISKLFVVVNKMDDPSVEWAKERFDEIEKKISPFLKSCGYNVKKDVLFLPISALHGTNIKEKIPKNVCGWWNGPTLFEGLDSIELPPRDPKGPFRMPLIDKFKDMGTVVMGKIESGTVRQGDSLIIMPNKVQVKVVAVYRESDEIKYARPGENVRVKLSGVEEDDISTGFVLSSISNPIQAVLEFEAQLQILELLEHKAIFTAGYKAVLHIHSVVEECEIMELLEQMDPRTKKPMKKKCLFVKTGAVVLCRIQVNDTICVEKFSDFPQLGRFTLRDEGKTVAIGKVVKLLTATGHE*

Protein Length: 666

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCATCTTCGTCGTGACCTTGGCTGTCACTCAATTCGCAAACTGTGCCCTTTACTAATCGATGTACAATTTTTGACATCCGCAATTTAAAAGATTTTCAGATCCGCTTTAAGGCCGCAAAGAGTGACGATAACTCAGGAAAGAAGCACAGAATCACTAGGCCGACCGGGACGTTCATTTTGTAGGTTGTTGGTGAACTGACGGAACTCCGATCCTAAAAAACAAACCGCAGA_-_ATGGCTGAACCAGACTCGTGGGAGGAGATGGATTCTCAGCCTTCGCATTCTGCAGCTGCCGCATCATCGATGATGTCAAAATTGAACGCAAATGCCCCGTCGTTTACATTTAATGTCAAAGCCCCTGCTTTTTCACCGGTTCCGAACGCGTATTTTCCCGCACAACCTAGTACACAACAGGCCTTTTTTCCGACCAATCCTGTGAACTCCACCAATCCTCAATTTGTAAACCCTACAAGCCCCATACAACCTATCAGCCCTACAAACCCCTCTGCAGGGTTGCCCCCACAGAACGCACCGGGGGCCGTTTCACCATACGTCCAACCCTCATATCCTCCTGCGGTCGACCATCAAGTGGCGGCTAGAAATGACGTTGCCTCTAAAATTGAAACCCTGCGGCATTCTGAGCGGGAGCGGTATGAAACGAGGCCGGACTTAACCGATAAGGATGTTGAGATGGAAGTGGATGGTGGATCTGCCCAAGTCGAAGCAAAAGAAACGGCTGCCGCCAACGGCGAGAGTAACGGTGCGTTGAGAGGGGACGAGGAGCAGGTAGTATCTAATTTGAACTCATTGAAATTAAAGGACACAGAACAGAATAAAGAAGTTGACATTAAGCATGAAGAAATGGCTCAAGAACATTTTGAGGATTCCCAACAATTGGAAGAGGAAGAGGAGATTGAAGAGAAGCCACACATGAATGTTGTCTTTATTGGTCATGTTGATGCTGGTAAATCAACAACTGGTGGGCAAATATTATATCTATCTAAAATGGTGGATGAGCGTACAATTCAAAAATATGAAAAAGAAGCAAAGGAAAAGAACCGAGAAAGTTGGTATATGGCATATATCATGGACACAAATGAGGAAGAACGAGTAAAGGGAAAGACAGTGGAGGTTGGTAGAGCTCATTTTGAAACTGAGAAGACAAGATTTACAATCTTGGATGCTCCAGGGCACAAAAGCTACGTTCCAAACATGATTAGTGGTGCATCACAGGCTGATGTAGGAGTTTTGGTGATATCGGCTCGAAAGGGTGAGTTTGAGACTGGATTCGAAAGAGGTGGTCAGACAAGAGAGCATGCTATGTTGGCAAAAACATTAGGAATTTCTAAACTATTTGTGGTGGTCAATAAAATGGATGATCCTTCAGTTGAGTGGGCCAAGGAGAGGTTTGATGAAATTGAAAAGAAGATAAGCCCCTTCTTGAAGTCTTGTGGCTACAATGTAAAGAAAGATGTGCTGTTTCTTCCAATATCAGCACTTCATGGTACTAACATCAAAGAGAAGATCCCAAAAAATGTGTGTGGTTGGTGGAATGGTCCAACCCTTTTTGAAGGGTTGGATTCAATCGAATTACCTCCAAGAGATCCTAAAGGTCCTTTCAGGATGCCATTAATAGATAAATTCAAGGATATGGGAACTGTGGTTATGGGAAAAATAGAGTCAGGCACTGTAAGGCAAGGAGACAGCTTAATTATAATGCCAAATAAGGTTCAAGTGAAAGTTGTAGCTGTTTATCGTGAAAGTGATGAAATAAAGTATGCAAGGCCTGGAGAAAATGTTAGGGTGAAATTATCTGGTGTTGAAGAAGATGACATCTCTACAGGCTTTGTTTTGTCTTCCATTTCAAATCCAATTCAAGCAGTGCTGGAGTTTGAGGCTCAGTTACAGATATTAGAACTACTGGAACATAAAGCAATATTTACTGCTGGTTATAAAGCTGTACTGCATATTCATTCTGTTGTCGAGGAATGTGAGATAATGGAGCTGTTAGAGCAGATGGATCCAAGGACGAAAAAGCCAATGAAAAAGAAATGTCTTTTTGTTAAGACTGGAGCAGTTGTGTTGTGTCGCATTCAGGTAAATGACACTATCTGTGTTGAGAAGTTCTCTGATTTTCCTCAACTTGGAAGGTTCACACTTCGGGATGAAGGTAAAACAGTCGCAATTGGAAAAGTTGTGAAGCTCCTAACTGCCACAGGACATGAATAA___CCGACGGAAATTGACTGAGACTATCCCTCGGCAATTATTCGATATGCACTAATAGAATGAGTGGGGCCTCGAGGGATTCTTTAATTCATCTTTCTGCACAGATTATGTGAGGCAACCCTTGAAATCGTTTCAATTTTCAGGTGCAAGGTGAACATGATGTCAGTAATCTTTATTTCTTGAACTGTCTTCAAGTGAAGAATGATGCAAGGGATGGATAAAGCGTTCATTGTGGTAGCAGTGAAGGTTTAGATATATATAGAATGATTGCTATTTTTGCAAGCCTCTATTCTGCCATGTCATGGGGGGCATTTAAACAAGAATACACAGATTTAACAGATTTAAGTGAACCTCAATTATAGGTCTGTTTGTATCTGGTCTATATTTTGTTCAATTTTATGTGGACGTAAGAGTTGTAATATTTTCAGCAATTTCATGTGCTCTAATGGCTTGAACATTGTTTGTTCAGCTTATATAAAGACACTGAATTTTCAACGATTTTACCGTTCTAATAGCTTGAGCATTATCTGTTCAGCTAATATTAAGGCACTGAATTATTTGTTTATCAGCGTTCTATGGTCAAGCCATTTCTCGTGATCTTGTGGCTTGGGCATTATATGTTCAGCTGACATTAGAGAGTTAATGGCTTTTAATCACCATTCATTTATTTCATGTTCTTGTTTTGAATATTTGGAACTATGATTTGCCCTGGTTGCTCAATGTATATAAACTTCTGGGGTAAACCCAAAAATTCTGTTGCCATGA

Fln Alignment:

AllPineV4_c62008___EQNKEVDIKHEEMAQEHFEDSQQLXXXXXXXEKPHMNVVFIGHVDAGKSTTGGQILYLSKMVDERTIQKYEKEAKEKNRESWYMAYIMDTNEEERVKGKTVEVGRAHFETEKTRFTILDAPGHKSYVPNMISGASQADVGVLVISARKGEFETGFERGGQTREHAMLAKTLGISKLFVVVNKMDDPSVEWAKERFDEIEKKISPFLKSCGYNVKKDVLFLPISALHGTNIKEKIPKNVCGWWNGPTLFEGLDSIELPPRDPKGPFRMPLIDKFKDMGTVVMGKIESGTVRQGDSLIIMPNKVQVKVVAVYRESDEIKYARPGENVRVKLSGVEEDDISTGFVLSSISNPIQAVLEFEAQLQILELLEHKAIFTAGYKAVLHIHSVVEECEIMELLEQMDPRTKKPMKKKCLFVKTGAVVLCRIQVNDTICVEKFSDFPQLGRFTLRDEGKTVAIGKVVKLLTATGHE
B8LNF8_______________EQDTEVDIKHEEMAQAHSEDSQQ--PEEEVEEKPHMNVVFIGHVDAGKSTTGGQILYLSKMVDERTIQKYEKEAKEKNRESWYMAYIMDTNEEERAKGKTVEVGRAHFETDKTRFTILDAPGHKSYVPNMISGASQADVGVLVISARKGEFETGFERGGQTREHAMLAKTLGICKLFVVVNKMDDPSVEWAKDRFDEIEKKISPFLKSCGYNLKKDVQFLPISALHGTNMKDRVPKNVCGWWNGPCLFEALDAIELPPRDPRGPFRMPLIDKFKDMGTVVMGKIESGTVRQGDSLFIMPNKVHVKVVAVYRESDELKYARPGENVRVKLSGVEEDDISTGFVLSSIAKPIHAVLEFDAQLQILELLEHKAIFTAGYKAVLHIHSVVEECEIMELLEQMDPRTKKPMKKKILFVKTGAVVLCRIQVNDTICVEKFSDFPQLGRFTLRDEGKTVAIGKVVKLPTITRNE

SNPs (tot: 63)

Position	A %	C %	G %	T %	Probability
1877	16			83	0.994512
1879		83		16	0.994512
1880		83	16		0.994512
1881	83			16	0.994512
2186	16		83		0.999991
2195		88		11	0.923564
2196		88	11		0.923564
2197	11			88	0.923564
2198			11	88	0.923564
2200	88		11		0.923564
2202		11		77	0.968132
2203	11			88	0.923564
2204			11	88	0.923564
2206	88	11			0.923564
2208	11			88	0.991528
2213		12		87	0.992554
2215	87		12		0.992554
2217	87	12			0.992554
2218			12	87	0.992554
2244			14	85	0.993533
2245		85		14	0.993533
2247	14	85			0.993533
2248	85	14			0.993533
2249	85			14	0.993533
2251			85	14	0.993533
2254	85		14		0.993533
2259			16	83	0.994512
2261		83	16		0.994512
2263		83	16		0.994512
2264	16			83	0.994512
2270	25			75	0.996473
2271	25			75	0.996473
2274		66		33	0.997452
2275		33	66		0.997452
2276	33		66		0.997452
2280	33	66			0.997452
2427	75		25		0.996473
2758	83	16			0.994512
2759		16		83	0.994512
2760	16	83			0.994512
2940	10		90		0.90298
2941	90		10		0.90298
2944	10			90	0.90298
2967	77		11	11	0.968132
2968	88		11		0.923564
2969	88	11			0.923564
2970	11		88		0.923564
2973	11	11	77		0.968132
2982			20	80	0.999996
2984		20		80	0.999996
2985	20		80		0.999996
2989	80	20			0.999996
2990			10	60	1.0
2991			11	88	0.92373
2992	28		71		1.0
2993	71			28	1.0
3004	75			25	1.0
3006	25		75		1.0
3008	25	75			1.0
3009	87		12		0.992555
3010	37	62			1.0
3011			87	12	0.992555
3013	25	75			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain