SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene60088

Length: 1107 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene60088

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	78%
Blast2go	unknown [Picea sitchensis]	-	-	66%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NXT1

Fln msg: Separated hits, possible frame ERROR between 380 and 386,

Fln protein: MSATLSKRCLEPEALLDSTVIRESKRSFNGIGEETDLFRHLPQLDKxxxENEECASSEDFVSGVMRSLEEEISATCSTIYGPISGDNSSAYDICSVQEGQTLLSDSHIDLCYLLEASDDELFIPSSPPILDSKNEFCQPSKETFNAEGLSETPDLKSQGENWDFVEEFQNYQQFALLENTWDASHAQDYTNKDFVSQDMFFEGDFSAAWTMETAGYM*

Protein Length: 219

Fln nts: ATGGGCTGCCATTTTATTCTTCAACCTCCCTTGCCGTTTGCCAGACTCTTCCGACTAAAGCCAGATCACACAGCTATATAAATATATAGCTTCCCCTGTGTTTCATGCAGGAAATCCTGCTATAGCCTGTATCACATCTGCTCAATTATACATATTGTCAGTTAGAGCAGCTCTTCGCCGCAGCCGAACTATCATAACCGTTGTGTTCCGTGTTCGAATCTCTGGTCCGTCTTTCAACG_-_ATGTCTGCCACGCTCAGCAAGCGGTGCCTAGAGCCGGAGGCTCTGCTGGACTCCACTGTTATCCGGGAGTCCAAACGCTCTTTCAATGGCATTGGCGAAGAAACGGATCTGTTTCGTCATTTGCCACAGTTGGACAAAACxxxxxxAGAAAATGAAGAATGCGCTTCCAGTGAGGATTTCGTAAGTGGTGTGATGAGAAGCCTGGAGGAAGAGATCAGCGCCACATGCTCCACTATTTATGGTCCCATCTCGGGGGATAATTCGTCGGCCTACGATATTTGTAGTGTCCAGGAGGGACAAACTCTACTTTCGGACTCACATATTGACCTGTgTTACCTCCTAGAGGCTTCAGACGACGAGCTTtTCATTCcCTCTAGCCCCCCCATTCTGGACTCTAAGAACGAGTTCTGTCAACCTTCTAAGGAGACCTTCAACGCCGAGGGTTTGTCCGAGACCCCCGATCTGAAATCCCAGGGTGAAAATTGGGATTTTGTAGAAGAATTTCAGAATTATCAACAGTTTGCTTTGTTGGAAAATACGTGGGATGCGAGCCACGCGCAGGATTATACGAACAAAGATTTTGTGAGCCAAGACATGTTCTTTGAGGGGGATTTTTCTGCGGCATGGACAATGGAAACTGCCGGTTATATGTAA___TCAACCCGCTGCTCGCAACATTTCTTAGACGAAGACACGCGCTCTTTGAATCGGTTGGAAAAGCTTACAGCAGACTTTCCAGAATTGTGAGACATATTAATTGAACAAGAGATTTTAACATCCCATTTTTCTACAGCCTGCAGTGAATATGAGTATCTAACCTGCTGCTCATAAAACTTCAGTGCTAGTAGGAGTCTTTCCATCTGAAAGATAAC

Fln Alignment:

AllPineV4_c61668___MSATLSKRCLEPEALLDSTVIRESKRSFNGIGEETDLFRHLPQLDKTxxENEECASSEDFVSGVMRSLEEEISATCSTIYGPISGDNSSAYDICSVQEGQTLLSDSHIDLCYLLEASDDELFIPSSPPILDSKNEFCQPSKETFNAEGLSETPDLKSQGENWDFVEEFQNYQQFALLENTWDASHAQDYTNKDFVSQDMFFEGDFSAAWTMETAGYM
A9NXT1_______________MSDTLRKRCREPEALPDSTDVPESKR-FNGIGDETDLFRHLPLLDKTxxENEECASSEDFVIGVMRSLEEEISATCSTFYAPTSGDNSSAYDICSVHESQTLVSGSDIDLCYLLEASDDELGIPSSP-VLDSKDKFCQPSKETFPAEGLSGTPNLKCQGENWDFVDEFQNYQQFALFEDAWDANHVQDYMNEDFISQDMLFEGEFSAAWTLETAGCM

SNPs (tot: 45)

Position	A %	C %	G %	T %	Probability
98	25		75		0.996474
107	33		66		0.997452
111		50	50		1.0
116		66		33	0.997452
142			50	50	1.0
174		33		66	0.997452
201		33		66	0.997452
220	33	66			0.997458
226		33		66	1.0
239		33	66		1.0
246	33		66		0.997452
257	33	66			1.0
398			33	66	0.997476
570	50		50		1.0
603			40	60	1.0
604			50	50	1.0
610	40	60			1.0
656	14			85	0.993533
657		28		71	1.0
658		85	14		0.993533
659			14	85	0.993533
660	85		14		0.993533
673	85	14			0.993533
695		63	36		1.0
709		63	36		1.0
710		63	36		1.0
768	20		80		0.999999
803	80		20		0.999999
816		88		11	0.991528
825			14	85	0.993533
826	14		71	14	0.984337
885		83	16		0.994512
892	83			16	0.994512
896	83		16		0.994512
924	83		16		0.994512
936		20	80		0.995493
937		80	20		0.995493
939		80		20	0.995493
961		75		25	0.996473
984	20		80		0.995493
1001	80	20			0.995493
1004	80			20	0.995493
1005	80		20		0.995493
1028			20	80	0.995493
1041	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain