SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene35821

Length: 1133 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene35821 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	CBS; Cl- channel, voltage gated; Twin-arginine translocation pathway signal [Medicago truncatula]	-	-	69%
FL-Next	sp=Chloride channel protein CLC-c; Arabidopsis thaliana (Mouse-ear cress).	-	-	68%
Blast2go	cl-channel clc-7	-	-	82%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Cystathionine beta-synthase, core	IPR000644	-	-
Sma3	Chloride channel, voltage gated	IPR001807	-	-
Sma3	Chloride channel ClC-plant	IPR002251	-	-
Sma3	Twin-arginine translocation pathway, signal sequence	IPR006311	-	-
Sma3	Chloride channel, core	IPR014743	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: sp_plants

Fln subject: Q96282

Fln msg: Distance to subject end: 512 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 269 - 779

Fln protein: MNREVRMAREVKDSGCEPKPDEHPLSEIGICEPLLANSKQQKQRMSRSISNATSQLAIVGSNVCPIESLDYEIIENDLFKHDWRSRTQIQIFQYIILKWTLAFLIGLLTGLVGFTINLAVENIAGTKLAITNNFMLQKRYVLAFGVYAGSNFILVIFAAILCAYIAPAAAGSGIPEVKAYLNGIDAPGILSPSTLIVKILGSICAVSGSLYVGKEGPLVHTGACIASLLGQGGSKRYHLTWRWLRYFKNDRDRRDLITCGSAAGVAAAF

Protein Length: 270

Fln nts: ATGGGGCCGTCAAACTTAATTAATACTTTCCTTTACTTGATAATACTCTCTGCTTGCTGTGTTAATGGTGATCAATACCACTACCCAGAGTAACCAATCAATCAGTTGTCACAGATCGGCAGAATCGGTTGTCAAAAACACCCATTCCAACACGGCAGCAGTTTCAATTATTGAATCAATGCATATAGGGAACCGTCGTGACCTTGGCTGTCACTCGTTCTGGTTACTCTATCTATCACCCATTCATTATTTGATGTGATTGCCAGTCTCCCGCCCCAAATGACCACATCTCTTTTGCTCCTCGCCGGTTAATGCCGGTGTTAG_-_ATGAACAGAGAAGTGAGAATGGCAAGAGAAGTAAAAGATTCAGGTTGTGAGCCTAAGCCTGATGAGCATCCCCTTTCAGAGATTGGAATATGCGAACCATTATTAGCGAACTCGAAGCAGCAGAAGCAAAGGATGAGCCGATCCATTTCCAATGCGACCTCGCAGCTAGCCATTGTGGGTTCAAACGTTTGTCCTATCGAGAGCTTGGATTACGAGATTATTGAAAATGATCTCTTCAAACATGATTGGCGTAGCCGCACACAGATTCAAATATTTCAGTACATCATTCTGAAATGGACTCTGGCATTTTTAATTGGCCTTCTAACTGGACTTGTTGGCTTCACCATCAATCTAGCAGTTGAAAACATTGCTGGGACCAAGCTAGCAATTACCAATAATTTCATGCTACAGAAAAGGTATGTATTAGCATTTGGAGTATATGCCGGATCTAATTTTATTCTAGTTATATTTGCAGCAATACTTTGTGCCTATATTGCTCCAGCGGCTGCCGGATCTGGTATACCAGAAGTTAAAGCTTATTTGAATGGTATTGACGCTCCAGGAATTCTGTCTCCAAGCACACTGATAGTGAAGATTCTAGGTAGCATTTGTGCGGTCTCTGGTTCCTTATATGTAGGTAAGGAGGGACCTCTTGTGCATACTGGGGCATGCATAGCATCACTGTTGGGGCAGGGGGGCTCCAAACGGTACCATTTAACATGGAGATGGCTGCGGTATTTCAAAAATGATAGAGATAGACGTGATCTCATAACATGTGGTTCTGCTGCTGGAGTTGCTGCTGCATTTCG

Fln Alignment:

AllPineV4_c28569___IGICEPLLANSKQQKQRMSRSISNATSQLAIVGSNVCPIESLDYEIIENDLFKHDWRSRTQIQIFQYIILKWTLAFLIGLLTGLVGFTINLAVENIAGTKLAITNNFMLQKRYVLAFGVYAGSNFILVIFXXXXXXXXXXXXAGSGIPEVKAYLNGIDAPGILSPSTLIVKILGSICAVSGSLYVGKEGPLVHTGACIASLLGQGGSKRYHLTWRWLRYFKNDRDRRDLITCGSAAGVAAAF
Q96282_______________VGFRQPLLARNRK----------NTTSQIAIVGANTCPIESLDYEIFENDFFKQDWRSRKKIEILQYTFLKWALAFLIGLATGLVGFLNNLGVENIAGFKLLLIGNLMLKEKYFQAFFAFAGCNLILATAAASLCAFIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFGVAAGFVVGKEGPMVHTGACIANLLGQGGSKKYRLTWKWLRFFKNDRDRRDLITCGAAAGVAAAF

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
607	75		25		0.996479
617			14	85	0.993533
825	40		60		1.0
836	25		75		0.996473
929	33			66	0.997452
930	33		66		0.997452
931	66			33	0.997452
934	33		66		0.997452
935		66		33	0.997452
936		66		33	0.997452
938	75		25		0.99649
939		25	75		0.99649
943	66			33	0.997452
944		66		33	0.997452
945			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain