SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene35103

Length: 973 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene35103 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	clathrin adaptor complexes medium subunit family protein [Arabidopsis thaliana] gb\|AAL58937.1\|AF462850_1 At2g20790/F5H14.24 [Arabidopsis thaliana] gb\|AAL90943.1\| At2g20790/F5H14.24 [Arabidopsis thaliana] gb\|AEC07073.1\| clathrin adaptor complexes medium su	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	64%
Blast2go	clathrin adaptor complexes medium subunit family protein	-	-	76%

Source	InterPros	Term	Type	Identity
Sma3	Clathrin adaptor, mu subunit, C-terminal	IPR008968	-	-
Sma3	Clathrin adaptor, mu subunit, conserved site	IPR018240	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: tr_plants

Fln subject: D7T6N6

Fln msg: Separated hits, possible frame ERROR between 235 and 285, Distance to subject end: 195 aas, your sequence is shorter than subject: 324 - 627

Fln protein: WPLVLHIKGQYRILVLPLVEPYHLMMYKKICQRDDCGNPNEVKKNSFPGESLSSLLLDLPCITGAFMVAQALRDVVTxxxxxxxxxxxxxxxxxxGCHSTLTGSMGIAGIAARAKPVGTPVAATATVSSTATGTTTSDLPGSKGSSRTVDKDALRNFISSSMPFGTPVDLGVANLSATRLNGFSSLDIPHTDLKQPAWKPYLFRGKQRILFMIHEVVSAAMYDRDDVPDTISISGQINCRADLEGLPDVSFPLTSLNTAHIEALSFHPCAQVPEQGVDKQALIFSPPLGNFILMHYQASLCNIGPPIKGFYQLSMVSEDEGAFL

Protein Length: 325

Fln nts: GTGGCCTCTAGTTTTGCACATTAAGGGGCAGTATCGTATTCTGGTTCTTCCATTGGTAGAACCTTATCACTTGATGATGTACAAGAAAATATGCCAAAGGGATGACTGCGGAAATCCAAATGAAGTTAAGAAAAATAGCTTCCCAGGCGAAAGTCTATCATCGCTCTTACTTGATCTTCCATGCATCACAGGGGCATTCATGGTGGCGCAAGCCTTGCGAGATGTGGTTACTGGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTGGCTGTCACTCAACTTTAACTGGAAGCATGGGGATAGCGGGCATTGCAGCTAGGGCTAAGCCTGTAGGAACACCTGTTGCAGCTACAGCAACTGTCAGTTCTACTGCTACAGGCACTACGACATCAGATCTTCCTGGTAGCAAAGGTAGCTCCAGAACAGTGGATAAAGATGCCCTGAGGAACTTCATCAGTAGTTCAATGCCTTTTGGTACTCCTGTTGACCTTGGTGTTGCTAATCTTTCTGCTACGAGATTGAATGGGTTTTCATCATTGGACATACCTCATACAGATCTAAAACAGCCAGCATGGAAGCCTTACCTGTTCAGAGGAAAGCAGAGGATATTGTTTATGATTCATGAAGTCGTATCTGCAGCAATGTATGACAGGGATGATGTTCCAGATACTATATCCATTTCTGGTCAAATAAATTGTCGAGCTGATTTAGAAGGTTTGCCAGATGTTTCTTTTCCCTTAACAAGCCTGAATACAGCTCACATTGAAGCATTATCTTTCCACCCATGTGCACAGGTTCCTGAGCAAGGTGTGGACAAGCAAGCTCTAATATTTTCACCGCCGCTTGGAAACTTTATTTTAATGCATTACCAGGCTTCTCTGTGCAACATAGGTCCTCCAATTAAAGGTTTTTACCAGCTTTCTATGGTCTCAGAAGACGAAGGTGCATTTTTGTT

Fln Alignment:

AllPineV4_c26047___WPLVLHIKGQYRILVLPLVEPYHLMMYKKICQRDDCGNPNEVKKNSFPGESLSSLLLDLPCITGAFMVAQALRDVVTGxxxxxxxxxxxxxxxxxGCHSTLTGSMGIAGIAARAKXXXXXXXXXXXXXXXXXXXXXSDLPGSKGSSRTVDKDALRNFISSSMPFGTPVDLGVANLSATRLNGFSSLDIPHTDLKQPAWKPYLFRGKQRILFMIHEVVSAAMYDRDDVPDTISISGQINCRADLEGLPDVSFPLTSLNTAHIEALSFHPCAQVPEQGVDKQALIFSPPLGNFILMHYQASLCNIGPPIKGFYQLSMVSEDEGAFL
D7T6N6_______________WPLILHMKGHYCILVLPLVEPQHLKAYAGVCRRSDCGNAIGV-----PG-SLSSLLFDLPSITGACMVAHAIGDVITGxxxxxxxxxxxxxxxxxGLLDSLTGSIGISGISTRTKPVAAPVAASTTSSTAVIGAVTSDAP--KFGSRPLDKDALRTFINSSMPFGTPLDLSYSNIFAIKVNGFSSSDLPLPDLKQPAWKPYLYKGKQRMLFTIHETVHAAMYDRDEIPDSISISGQVNCRAELEGLPDVSFPLTGLNKAGIEVLSFHPCAQVPEQGVDKQAVMFSPPLGNFVLMHYQA-FCGLGPPVKGFYQLSMVSEDEGAFL

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
264		25		75	0.996473
265		25		75	0.996473
266	75	25			0.996473
267		25		75	0.996473
268	25			75	0.996473
284	80	20			0.995493
285		20		80	0.995493
286	80	20			0.995493
287	20		80		0.995493
346		85		14	0.993533
347	85		14		0.993533
350	85		14		0.993533
353		85	14		0.993533
354	14			85	0.993533
356	14			85	0.993533
359		14		85	0.993533
360	14			85	0.993533
361	14			85	0.993533
362		85	14		0.993533
363		85	14		0.993533
366	14			85	0.993533
367		14	85		0.993533
368	85		14		0.993533
421			33	66	1.0
587			75	25	0.996476
693	66	33			0.997476
694		33	66		0.997476
695			66	33	0.997476
696	33			66	0.997476
701	66		33		0.997464

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain