SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene34807

Length: 1039 nt

Origin of reads: Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene34807

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=C0PT44_PICSI	-	-	60%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	60%
Blast2go	pectin methylesterase-like protein	-	-	70%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Peptide chain release factor class I/class II	IPR000352	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Peptide chain release factor 1	IPR004373	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Peptide chain release factor	IPR005139	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Carbohydrate-binding/sugar hydrolysis domain	IPR006633	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: C0PT44

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 85 aas, your sequence is shorter than subject: 346 - 601

Fln protein: *VL*NGGRQLTFRLGSAALTNQATCVESLQEASGTLQNILSKSVLDVSRVVSNSLALVKNIPVIGKASNTPPLHSRRLFSNPTSHERHNQVDVDDEFPSWLPASDRRRLLQTSEAAGNVVTVAQDGSGNYTTITDALNAAPNNSLDRYVIHITNGVYQEYLEVPKHKKNIMLVGDGINATVITGNRSVVDGWTTYRSATVAVAGQGFLARDITFENTAGPEKHQAVALRVASDLSAFYRCSFKGYQDTLYAHSLRQFYRECEIYGTVDFIFGNAAAVFQNCNLLARKPMDNQKNCFTAQGRDDPNQNTGISIQNCTVSAAPDLAPVKSSFQTFLGRPWKNYSRTVY

Protein Length: 347

Fln nts: CTAAGTACTTTGAAATGGAGGCAGGCAGTTGACATTCAGACTTGGTAGCGCAGCTCTTACTAATCAAGCTACCTGCGTCGAAAGTCTCCAGGAGGCCAGCGGAACCCTGCAAAACATACTGAGTAAGAGTGTTCTTGATGTGTCACGAGTCGTAAGCAATTCTTTGGCTCTTGTCAAAAATATTCCTGTCATTGGCAAAGCTTCAAATACGCCTCCTTTACACAGCCGACGCCTGTTCTCCAATCCAACGAGTCATGAGCGTCATAATCAAGTTGATGTAGATGATGAATTCCCTTCATGGTTGCCTGCTTCAGACAGAAGGAGACTTCTTCAGACCTCCGAGGCAGCCGGCAATGTTGTTACGGTGGCTCAGGACGGAAGTGGTAATTATACCACAATTACTGACGCGCTGAATGCTGCCCCAAACAATAGTTTAGATAGATATGTGATACATATTACAAATGGAGTCTACCAGGAATATCTTGAGGTACCCAAACACAAAAAAAATATCATGCTGGTGGGAGATGGCATAAATGCCACAGTGATCACCGGCAATAGAAGCGTTGTGGACGGATGGACTACtTACCGCTCTGCgACAGTCGCTGTAGcTGGGCAAGGCTTTCTCGCTCGGGATATCACTTTCGAGAACACGGCGGGCCCAGAGAAGCATCAGGCTGTCGCTCTCCGCGTTGCCTCGGATCTATCAGCATTCTACCGCTGCAGCTTCAAAGGTTACCAAGACACTCTTTACGCACACTCTCTTCGACAATTtTACcGGGAATGTGAGaTTTACGGcACAGTTGATTTTATCTTtGGCAACGCTGCAGCTGTGTTTCAAAACTGcAATCTCCTGGCTAGGAAACCGATGGAtAATCAAAAGAATTGtTTCACGGCTCAAGGAAGAGATGACCCAAATCAgAACACCGGAATCTCTATACAAAATTGCACCGTCTCTGCTGCGCCTGATCTCGCTCCAGTGAAGAGTTCTTTCCAGACATTCTTGGGGAGGCCATGGAAAAATTACTCCCGCACAGTTTAT

Fln Alignment:

AllPineV4_c24968___SAALTNQATCVESLQEASGTLQNILSKSVLDVSRVVSNSLALVKNIPVIGKASNTPPLHSRRLFSNPTSHERHNQVDVDDEFPSWLPASDRRRLLQTSEAAGNVVTVAQDGSGNYTTITDALNAAPNNSLDRYVIHITNGVYQEYLEVPKHKKNIMLVGDGINATVITGNRSVVDGWTTYRSATVAVAGQGFLARDITFENTAGPEKHQAVALRVASDLSAFYRCSFKGYQDTLYAHSLRQFYRECEIYGTVDFIFGNAAAVFQNCNLLARKPMDNQKNCFTAQGRDDPNQNTGISIQNCTVSAAPDLAPVKSSFQTFLGRPWKNYSRTVY
C0PT44_______________SAAITNQYTCLDS--SARSNLRQELQGGLMSISHLVSNSLAIVKNIATRASNVTVNSIHNRRLLSDDQGSEF--MAMESDGFPSWMSAKERSLLQSSRDNIMPNAVVAKDGSGHHTSIGDAVNAAPQKSRTRYVIHIKAGIYWENVEVNKKKTHLMFIGDGIGATVVAGNRNVKDGYTTYRSATVAVNGNGFIARDITFENTAGAAKHQAVALRVGSDFSAFYRCSFQGYQDTLYVHSLRQFYRECNVYGTVDFIFGNAAVVLQNCNLFARKPLANQQIVYTAQGRQDPNENTGISIQNCQVIAASDLIPVKRSFPAYLGRPWRQYSRTVF

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
377	33		66		0.997452
613		50		50	1.0
623	40	60			1.0
746		75		25	0.996473
776		33		66	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain