SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene33572

Length: 1357 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene33572

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	cysteine-rich receptor-like protein kinase 2 [Arabidopsis thaliana] sp\|Q9CAL3.1\|CRK2_ARATH RecName: Full=Cysteine-rich receptor-like protein kinase 2; Short=Cysteine-rich RLK2; Flags: Precursor gb\|AAG52471.1\|AC010796_10 putative protein kinase; 37247-3480	-	-	35%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	54%
Blast2go	cysteine-rich receptor-like protein kinase 2	-	-	53%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	53%
Blast2go	protein kinase activity	GO:0004672	Molecular Function	53%
Blast2go	cell part	GO:0044464	Cellular Component	53%

Source	InterPros	Term	Type	Identity
Sma3	EGF-type aspartate/asparagine hydroxylation site	IPR000152	-	-
Sma3	Toll/interleukin-1 receptor homology (TIR) domain	IPR000157	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	S-locus glycoprotein	IPR000858	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Chaperonin TCP-1, conserved site	IPR002194	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Peptidoglycan-binding Lysin subgroup	IPR002482	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Apple-like	IPR003609	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	PAN-2 domain	IPR013227	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Peptidoglycan-binding lysin domain	IPR018392	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: C0PPW7

Fln msg: Overlapping hits, possible frame ERROR between 860 and 848, Unexpected STOP codon at 3' end. Distance to subject end: 92 aas, your sequence is shorter than subject: 404 - 702

Fln protein: GTTKTAPNPKLLGAVAVDAPSDLMENTYGLAECWADLLERAADRV*FLHGNIYSHAKLELLVHDSDLRPAACVTRFSEGKRFSVLGISLGVVATMIVLIAVIGTWKRNICSPLKCWEGISPTGEPELAGEEEDSIVTPTIANPELIFKYYILRQITSNFEAENKLGNGGFGSVFKGVLPNGREVAAKLLFMGPRQAYAEYLNEANLISRVQHKNLVKLLDCSVDGSRRLLVYEYLHNSSIDKIAFDATKRHLLKWKERYEIIVGTVGGVAYLHGESHIGVVxxxxxxSNILLDYKQGPKIADFGLARFFAEDQSHVSTRVAGTL*QSSPTVPD*LF*KN*SN*SNYNSYI*IWILQF*GG*VVVI*LHMKFCRGYMVPEYALCGQLKETAGYDYEILQRLHGG*

Protein Length: 405

Fln nts: ATGGGACCACTAAAACTGCACCAAACCCCAAACTCTTGGGTGCAGTTGCAGTAGACGCCCCTTCTGATTTAATGGAGAATACCTACGGCTTAGCGGAGTGTTGGGCAGATCTGCTTGAACGGGCTGCAGATCGTGTGTAATTTTTACATGGGAATATTTATTCTCATGCCAAACTGGAGCTGTTGGTGCACGATTCAGATCTAAGACCTGCTGCCTGCGTTACCAGATTTTCAGAGGGTAAAAGATTTAGCGTGTTGGGCATCAGTCTGGGCGTAGTAGCCACTATGATTGTCTTAATTGCAGTCATAGGCACTTGGAAACGGAATATTTGCTCTCCTCTGAAATGTTGGGAAGGGATTTCTCCAACTGGAGAACCTGAACTTGCAGGTGAAGAGGAAGATTCTATCGTAACGCCGACTATAGCGAATCCAGAACTCATTTTCAAATACTACATCCTGAGACAGATTACCTCCAATTTTGAAGCAGAGAACAAACTTGGCAATGGAGGGTTTGGATCCGTCTTTAAAGGGGTTCTCCCTAATGGCAGAGAGGTTGCAGCTAAACTGCTGTTTATGGGTCCAAGGCAAGCATATGCTGAATATTTGAATGAAGCAAATCTCATCAGTCGTGTTCAGCACAAGAATCTAGTGAAACTGCTTGATTGCTCCGTTGATGGTTCCAGAAGGTTACTAGTTTATGAATACCTCCACAACAGTAGTATTGACAAGATTGCATTCGATGCAACAAAACGTCATCTACTTAAATGGAAAGAGAGGTATGAAATCATAGTTGGAACTGTAGGTGGTGTGGCCTATCTTCACGGGGAATCTCATATTGGGGTAGTCxxxxxxxxxxxxxxxxxxAGCAATATTTTACTAGATTATAAACAAGGACCCAAAATAGCAGACTTTGGGTTGGCCAGATTCTTTGCGGAAGATCAAAGCCATGTTAGCACTAGAGTTGCAGGAACTTTGTAGCAATCTTCTCCTACCGTTCCCGACTAATTGTTTTAAAAAAATTAGAGCAACTGAAGCAATTACAACTCATATATCTGAATATGGATCCTGCAATTTTGAGGCGGCTAGGTTGTGGTGATTTGATTGCATATGAAATTCTGCAGAGGTTACATGGTGCCAGAGTACGCCTTATGCGGACAGTTGAAGGAAACGGCAGGGTACGATTATGAAATTCTGCAGAGGTTACATGGTGGCTGAGTAAAGGCAGGCTACAATTTTGCCCGGAATGTTATTTTCAATAGTAATTTGGATGTTTTTTCATAACATTTGACGAATGGAGTGTTTGAATTGATTTTGAGTGTGAGTATTGTCTATCAATTCAATAGTAATAAGGACTTACTC

Fln Alignment:

AllPineV4_c21130___TTKTAPNPKLLGAVAVDAPSDLMENTYGLAECWADLLERAADRV*FLHGNIYSHAKLELLVHDSDLRPAA------CVTRF----------------------SEGKRFSVLGISLGVVATMIVLIAVIGTWKRNICSPLKCWEGISPTGEPELAGEEEDSIVTPTIANPELIFKYYILRQITSNFEAENKLGNGGFGSVFKGVLPNGREVAAKLLFMGPRQAYAEYLNEANLISRVQHKNLVKLLDCSVDGSRRLLVYEYLHNSSIDKIAFDATKRHLLKWKERYEIIVGTVGGVAYLHGESHIGVVHxxxxASNILLDYKQGPKIADFGLARFFAEDQSHVSTRVAGTL*QSSPTVPD*LF*KXXXXXXXXXXXXXXWILQF*GG*VVVI*LHMKFCRGYMVPEYALCGQLKETA
C0PPW7_______________TAKAAGSPKLFATDSVAGPSSSRGNIYSLAQCWRYLSRTDC-------GSCLTFAQETLLFGCHMGAPGAQFGSDKCYLRYEVYEFFTTSVLSPPPGESPPSSSEGKRPRVLGISLSVVAFTIVLIAAIGFWKWNFCSRAKRREEIYRTG-----GDEGDSVLMPTIANPELIFKFDILRESTSNFKAENKLGEGGFGSVFKGVLPDGREVAVKRLFMGTRQADAEFLNEANLISRVQHRNLVKLLGCSVEVSERLLVYEYLQNSSLDKILFDPTKRHLLDWKKRSEIILGTARGLAYLHEESDVRVIHxxxxASNILLDDKHRPKIADFGLARFFAEDQSHVSTRVAGTL-------------------------------------------------GYMAPEYALRGQLTEKA

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
252		75		25	0.996473
270	50		50		1.0
294		75		25	0.996473
314		75		25	0.996473
538	33	66			0.997467
542	66			33	0.997464
611	33		66		0.997452
1031	80		20		0.995493
1032	80			20	0.995493
1039	80	20			0.995493
1040			80	20	0.995493
1041		80		20	0.995493
1042	80		20		0.995493
1043	80		20		0.995493
1044		20		80	0.995493
1046	80		20		0.995493
1047		80		20	0.995493
1048	80	20			0.995493
1081			40	60	1.0
1100			40	60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain