SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene32386

Length: 1240 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene32386 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative receptor-like kinase (with alternative splicing) [Oryza sativa Japonica Group] gb\|ABF98430.1\| BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor, putative, expressed [Oryza sativa Japonica Group] gb\|ABF98431.1\| BRASSINOSTEROID I	-	-	78%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	brassinosteroid insensitive 1-associated receptor kinase 1 expressed	-	-	90%

Source	GOs	Term	Type	e value	Identity
Blast2go	kinase activity	GO:0016301	Molecular Function	0.0	90%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	90%

Source	InterPros	Term	Type	Identity
Sma3	IQ motif, EF-hand binding site	IPR000048	-	-
Sma3	Major intrinsic protein	IPR000425	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Myosin head, motor domain	IPR001609	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Calponin homology domain	IPR001715	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	Multicopper oxidase, copper-binding site	IPR002355	-	-
Sma3	Dilute	IPR002710	-	-
Sma3	Adenovirus E3 region protein CR2	IPR003470	-	-
Sma3	Leucine-rich repeat, typical subtype	IPR003591	-	-
Sma3	Pistil-specific extensin-like protein	IPR003882	-	-
Sma3	Myosin, N-terminal, SH3-like	IPR004009	-	-
Sma3	Tyrosine-protein kinase, active site	IPR008266	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Homeobox, conserved site	IPR017970	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	Dil domain	IPR018444	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PSK3

Fln msg: Overlapping hits, possible frame ERROR between 892 and 891, your sequence is shorter than subject: 344 - 606

Fln protein: VGQWKRFSWRELQLATDNFSEKNVLGQGGFGKVYKGILGDNTKVAVKRLTDYNSPGGEAAFLREVEMISVAVHRNLLKLIGFCITSSERLLVYPYMENLSVAYRLRELKPGEKGLDWPTRKQVAFGAARGLEYLHEHCNPRIIHRDLKAANILLDEDYEAVVGDFGLAKLVDAKKTHITTQVRGTMGHIAPEYLSTGRSSEKTDVFGYGIMLLELVTGQRAIDFSRLEEEEEVLLLDHVKKLQRDKRLDVIVDRYLKQNYNPMEVEAIIQVALLCTQTSPEERPKMTEVVRMLERHxxLAERWEEWQQQEVIRRREYALMPRRFEWAEDSTYNQEAIELSEAR*

Protein Length: 345

Fln nts: CGTCGGACAGTGGAAGAGGTTTTCTTGGCGTGAACTGCAGCTAGCAACTGATAATTTTAGTGAGAAAAATGTGCTTGGGCAGGGAGGTTTTGGTAAAGTGTACAAGGGAATACTTGGAGATAACACAAAGGTTGCAGTGAAACGCTTGACTGACTATAACAGCCCTGGTGGTGAAGCAGCTTTTTTGCGGGAAGTTGAGATGATTAGTGTGGCAGTTCACCGTAATCTTTTAAAATTGATAGGATTTTGTATTACTTCGTCGGAAAGGCTTCTTGTGTACCCTTACATGGAGAATCTCAGTGTTGCATATCGACTACGGGAACTCAAACCTGGGGAGAAAGGACTTGACTGGCCTACACGAAAACAGGTGGCTTTTGGTGCAGCTCGTGGTTTGGAGTACCTTCATGAGCACTGTAATCCAAGGATCATTCACCGTGACCTGAAGGCTGCCAATATTTTGTTAGATGAAGATTACGAAGCAGTTGTTGGTGATTTTGGATTAGCGAAGTTGGTTGATGCTAAAAAAACTCACATCACAACTCAAGTACGTGGAACTATGGGTCATATTGCTCCTGAATACTTATCAACTGGAAGGTCATCAGAGAAGACAGATGTGTTTGGATATGGTATTATGCTTTTAGAACTTGTCACAGGGCAGCGTGCCATAGACTTTTCTCGTCTTGAAGAAGAAGAGGAGGTGTTACTACTCGATCATGTCAAAAAATTGCAAAGAGATAAAAGGTTAGATGTCATTGTAGACAGATACCTCAAGCAGAATTATAATCCAATGGAAGTTGAAGCTATAATCCAGGTAGCATTATTGTGTACACAAACCTCACCTGAAGAACGGCCAAAGATGACAGAAGTTGTACGCATGTTGGAACGTCATxxxxxxCTAGCAGAACGCTGGGAGGAATGGCAGCAACAAGAAGTGATTCGTCGACGAGAATATGCACTGATGCCTAGAAGGTTTGAGTGGGCTGAGGATTCTACATATAACCAAGAAGCCATTGAACTATCTGAGGCAAGATGATGATGATCTGTCCGCTGTAAGGAACACTTCTTATTTGAAGCAGCTAATGCCTTTGTATCTAAATTTCTGAAAGTGACTCATTGAGAGCATAAGCTTGTCTCATTTTGTCCTTGAGTGTTTTATCACTGTGACAAAACTCCTATGAAATGTAAGATTACTAGACTTCCATTTGCTTGACATGTAATTTTTACCTTGGCTGATGTTCAAAA

Fln Alignment:

AllPineV4_c17157___GQWKRFSWRELQLATDNFSEKNVLGQGGFGKVYKGILGDNTKVAVKRLTDYNSPGGEAAFLREVEMISVAVHRNLLKLIGFCITSSERLLVYPYMENLSVAYRLRELKPGEKGLDWPTRKQVAFGAARGLEYLHEHCNPRIIHRDLKAANILLDEDYEAVVGDFGLAKLVDAKKTHITTQVRGTMGHIAPEYLSTGRSSEKTDVFGYGIMLLELVTGQRAIDFSRXXXXXXXXXLDHVKKLQRDKRLDVIVDRYLKQNYNPMEVEAIIQVALLCTQTSPEERPKMTEVVRMLERHxLAERWEEWQQQEVIRRREYALMPRRFEWAEDSTYNQEAIELSEAR
C0PSK3_______________GQLKRFSWRELQIATDNFSEKNVLGQGGFGKVYKGVLGDNTKVAVKRLTDYNSPGGEAAFLREVEMISVAVHRNLLKLIGFCITSSERLLVYPYMENLSVAYRLRELKPGEKGLDWPTRKQVAFGAARGLEYLHEHCNPKIIHRDLKAANILLDEYFEAVVGDFGLAKLVDAKKTHITTQVRGTMGHIAPEYLSTGRSSEKTDVFGYGIMLLELVTGQRAIDFSRLEEEDEVLLLDHVKKLQRDKRLDVIVDSNLKQNYDPMEVEAVIQVALLCTQTSPEERPKMTEVVRMLEGExLAERWEEWQQQEVIRRREYALMPRRFEWAEDSTYNQEAIELSEAR

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
965	20		80		0.999996
982	10			90	0.90298
983		90	10		0.90298
984		90		10	0.90298
1000	90	10			0.90298
1002	77	11	11		0.968132
1004	87			12	0.992554
1016	14			85	0.993533
1075	14	85			0.993533
1076			14	85	0.993533
1078		85	14		0.993533
1081	14	85			0.993533
1082	85		14		0.993533
1086	14		85		0.993533
1091		14	85		0.993533
1092	14			85	0.993533
1093		14		85	0.993533
1094	85		14		0.993533
1095	14			85	0.993533
1096	85	14			0.993533
1171	20		80		0.995493
1173	20	80			0.995493
1174		80		20	0.995493
1175	80			20	0.995493
1235		66		33	0.997461
1236		66	33		0.997461
1237	33			66	0.997452
1239	33			66	0.997457

SSRs (tot: 1)

Start position	End position	Sequence	Length
203	214	ATC ATC ATC ATC	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain