SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene32252

Length: 1502 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene32252 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=A9NUI7_PICSI	-	-	89%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	pectin methylesterase	-	-	72%

Source	InterPros	Term	Type	Identity
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NUI7

Fln msg: your sequence is shorter than subject: 421 - 559

Fln protein: TQQLNPRNIKDVKIWMSAALSYQYDCSSALKYVNTTQMVGRSMQELVTVMNFTSTALSMVDALDTYGKDMVIWKPPKTERTSNLSTTAHYYHHNNKIWDVSQVNELVPDVTVSKDESSMSIQQAVNSAPDYSERRFVIRIKAGVYEEIVRIPPSKTNLMFVGDGMDRTVIRGSMSVPSLPGVPTTYDSATVAVNADGFLARDIAFENAAGPGSHQAVALRVDSDLSAFYSCALLGNQDTLYSHTLRQFYRNCRIEGTVDFIFGNSAAVFENCVILVRPRQVNSSQGSSDAVTAHGRIDPAQPTGFVFHNCTINGTEEYMKDYYANPKIYKAYLGRPWKMYSRVIITNSYLGEIISPEGWLPWSGDFALDTLYYGEYQNYGPGATLSGRVPWSNQIPGGNVGMYSIESFIQGDEWLPGTIN*

Protein Length: 422

Fln nts: GGACTCAGCAGCTGAACCCAAGGAATATTAAAGACGTCAAGATCTGGATGAGCGCAGCGCTGAGTTATCAGTATGACTGCAGTTCGGCTCTCAAATATGTCAATACCACACAGATGGTGGGAAGATCCATGCAAGAGTTAGTTACAGTTATGAACTTCACTAGTACTGCTCTGAGTATGGTCGATGCTTTGGATACTTACGGGAAGGATATGGTCATATGGAAGCCTCCCAAAACTGAGAGAACATCCAATTTATCTACCACTGCACATTATTACCATCACAATAATAAAATTTGGGATGTATCACAGGTAAATGAATTGGTTCCCGACGTTACTGTTTCCAAGGATGAATCCTCCATGAGCATTCAACAAGCGGTGAACAGTGCACCGGATTATTCTGAGCGGAGATTTGTGATACGTATCAAGGCTGGGGTTTACGAGGAGATCGTTAGGATTCCTCCCAGTAAGACTAATCTCATGTTTGTGGGCGATGGCATGGATAGGACTGTGATCAGGGGGTCTATGAGTGTGCCGTCCTTGCCTGGTGTTCCCACTACATATGACTCTGCAACTGTTGCTGTAAATGCGGACGGTTTTTTAGCACGGGACATTGCATTTGAAAATGCAGCAGGACCAGGCAGTCACCAAGCAGTAGCCCTAAGAGTAGATAGTGATCTCTCTGCCTTCTACAGCTGTGCTCTTCTTGGCAACCAGGATACACTGTATAGTCATACTCTTCGCCAGTTCTACAGAAACTGCAGAATAGAAGGCACTGTTGACTTCATATTTGGTAACTCAGCAGCAGTTTTTGAAAACTGTGTTATTCTGGTGCGTCCTCGTCAAGTAAACTCAAGCCAAGGATCATCAGATGCTGTTACTGCGCATGGCCGAATAGACCCTGCTCAGCCCACAGGATTTGTTTTTCACAACTGCACCATCAATGGAACTGAAGAATACATGAAAGATTACTATGCCAATCCAAAAATTTACAAGGCGTACTTGGGTCGGCCATGGAAGATGTACTCCAGAGTGATTATCACGAACTCATACTTGGGAGAGATCATTAGCCCCGAAGGATGGTTGCCTTGGTCAGGGGACTTTGCTCTTGACACACTTTACTATGGGGAATACCAAAATTATGGGCCAGGTGCTACGCTTTCTGGAAGAGTGCCATGGTCTAATCAGATTCCTGGGGGGAATGTAGGAATGTATTCTATTGAAAGCTTCATACAGGGTGATGAGTGGCTACCAGGTACGATCAATTAA___TCCAAAAACAAAGGGTGATTAGTATGTATTTTCTGACGGACGTCCTTCCTGATATTGCTTCATCTCTGTAGTAGTACTGCAATTTCAACCAAAGTGATATTGCTTCATCTCCGGAGTAGTACTGCAATTTCAATTTGAGTTGTTATTGCTAATGAATATTTATATCTTTAGAGATGTTATCTCTTTGTTATTCTGATCGAAGTAAAAAGTATAAAGAGAAATATCTTTGTTATCTTT

Fln Alignment:

AllPineV4_c16859___TQQLNPRNIKDVKIWMSAALSYQYDCSSALKYVNTTQMVGRSMQELVTVMNFTSTALSMVDALDTYGKDMVIWKPPKTERTSNLSTTAHYYHHNNKIWDVSQVNELVPDVTVSKDESSMSIQQAVNSAPDYSERRFVIRIKAGVYEEIVRIPPSKTNLMFVGDGMDRTVIRGSMSVPSLPGVPTTYDSATVAVNADGFLARDIAFENAAGPGSHQAVALRVDSDLSAFYSCALLGNQDTLYSHTLRQFYRNCRIEGTVDFIFGNSAAVFENCVILVRPRQVNSSQGSSDAVTAHGRIDPAQPTGFVFHNCTINGTEEYMKDYYANPKIYKAYLGRPWKMYSRVIITNSYLGEIISPEGWLPWSGDFALDTLYYGEYQNYGPGATLSGRVPWSNQIPGGNVGMYSIESFIQGDEWLPGTIN
A9NUI7_______________TQQLNPRNIKDVKIWMSAALSHQYDCSSALKYVNTTQMVGRSMQELVIVMNFTSNALSMVDALDTYGKDMVIWRPPKTERSSKLSSTADYSHHYNKIWDVLEVDDLVSDVTVSKDESSMSIQQAVNSAPDYSERRFVIRIKAGVYEEIVRIPPTKTNLMFVGDGMDRTVITGSMRVPSLPGVPSTYDSATVAVNADGFLARDIAFENAAGPVSQQAVALRVDSDLSAFYNCALLGHQDTLYTHTLRQFYRNCRIEGTVDFIFGNSAAIFENCLILVRPRQVNASKGSSDAVTAHGRTDPAQPTGFVFHNCTINGTEEYMKEYYSNPKIYKAYLGRPWKMYSRVIFMNSYLGELIVPEGWMPWTGDFALDTLYYGEYQNYGPGAKVSGRVPWSNQIPKINAGKYSINSFIQGDEWLPATIN

SNPs (tot: 52)

Position	A %	C %	G %	T %	Probability
1078	33		66		1.0
1079	66		33		1.0
1087	37		62		1.0
1090	66		33		1.0
1098		62		37	1.0
1102			66	33	1.0
1104		37		62	1.0
1106		62		37	1.0
1107	37		62		1.0
1114	62		37		1.0
1122			62	37	1.0
1123	37	62			1.0
1128	62		37		1.0
1130	33			66	1.0
1135	37	62			1.0
1155			66	33	1.0
1189	57		42		1.0
1195	42		57		1.0
1197		57		42	1.0
1203		62		37	1.0
1204		62	37		1.0
1215		57		42	1.0
1218		50	50		1.0
1220	50		50		1.0
1226		33		50	1.0
1229	40	60			1.0
1240	50		50		1.0
1241	50			50	1.0
1242	50			50	1.0
1243	50		50		1.0
1250			40	60	1.0
1257		50	50		1.0
1265		40	60		1.0
1266		20		80	0.995493
1267	80	20			0.995493
1268			80	20	0.995493
1276		25	75		0.996473
1277	25		75		0.996473
1281	25			75	0.996473
1288	75	25			0.996473
1301		25		75	0.996473
1317		75		25	0.996473
1319		25		75	0.996473
1335	75		25		0.996473
1348	75		25		0.996473
1351		75		25	0.996473
1358			66	33	0.997452
1379	33		66		0.997452
1383		66		33	0.997452
1384			33	66	0.997452
1386	66		33		0.997452
1387		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain