SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene30650

Length: 1484 nt

Origin of reads: Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene30650 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	O-Glycosyl hydrolases family 17 protein [Arabidopsis thaliana] gb\|AAF02143.1\|AC009853_3 putative glucan endo-1-3-beta-glucosidase [Arabidopsis thaliana] dbj\|BAC42699.1\| putative beta-1,3-glucanase precursor [Arabidopsis thaliana] gb\|AAO64098.1\| putative g	-	-	47%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	39%
Blast2go	protein	-	-	69%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Ribosomal protein S7	IPR000235	-	-
Sma3	Glycoside hydrolase, family 17	IPR000490	-	-
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	Aliphatic acid kinase, short-chain	IPR000890	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Histone H2A	IPR002119	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	X8	IPR012946	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Ribosomal protein S2, conserved site	IPR018130	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AAN5

Fln msg: Separated hits, possible frame ERROR between 548 and 572, query STOP codon too far from subject stop. Distance to subject end: 111 aas, putative chimeric sequence, your sequence is shorter than subject: 357 - 462

Fln protein: MKGVGFFTMLLLLLFPLNSRAEIARKRSRVPSMGINYGRLGDDLPPPEQAVALIKSLGFGQVKIFDSDPNILNALANTSLRVVMAATNEELETLAASPAAAAEWLDQQVRPHLPAARIRMITVGNELLSHPEINQPRWPLLLPAMQNLQEAxxxxxxxxxIKVSTCIAMDALNVSYPPSAARFRADIADSVLRPVLQLLSSTKSYIFLNAYPYLAWSDDNNDIPLDYALFGPGATVVADGDLQYGDLLDAQVDSFVAAMEDIGLPGVKIAISETGWPTAGGPDEVGASVSNAALYNGRLVKKMMSNPPKGTPRRPGVGVPTFIFALFNENQKPGPVTERNWGLLYPNASHVYPLNF*

Protein Length: 358

Fln nts: GGGAGAATCTCTAAGAGATCTCAGGGTCGAATCCTTTTACCAGGAACACTcGTCAGCAAAATCgCTTCggATtATATTATCAGATTGCAATA_-_ATGAAAGGAGTAGGGTTCTTCACAATGCTGCTGCTGCTGCTTTTTCCGCTGAACTCCAGAGCAGAAATTGCCAGGAAGAGAAGCAGGGTGCCAAGCATGGGGATAAACTATGGGCGCTTAGGGGACGACCTGCCTCCGCCGGAGCAAGCAGTGGCGCTGATAAAATCTCTGGGCTTTGGACAGGTCAAGATCTTTGACTCCGAcCCGaACATTCTCAACGCTCTGGCCAACACCAGCCTCCGCGTGGTGATGGCAGCGACAAACGAAGAGCTCGAAACCCTAGCTGCCTCGCCCGCCGCCGCAGCAGAATGGCTGGACCAGCAAGTCCGGCCACACCTGCCCGCTGCCCGGATCCGCATGATCACGGTGGGCAACGAGCTCCTGAGCCACCCGGAGATCAACCAGCCGCGCTGGCCCCTGCTCCTCCCGGCAATGCAGAACCTCCAGGAAGCACTxxxxxxxxxxxxxxxxxxxxxxxxGATCAAAGTCTCCACCTGCATTGCCATGGACGCCCTCAACGTCTCATACCCGCCCTCCGCCGCAAGGTTCCGCGCCGACATCGCCGATTCCGTCCTCCGCCCGGTGCTCCAATTGTTGTCCTCCACCAAGTCCTACATTTTTCTCAACGCCTACCCCTACCTGGCGTGGTCCGACGACAACAACGACATACCGCTGGACTACGCTCTTTTCGGCCCCGGAGCCACTGTCGTGGCCGACGGGGATCTGCAATACGGTGACCTGCTGGATGCCCAGGTAGACTCCTTCGTGGCCGCCATGGAGGACATCGGGCTCCCAGGTGTGAAGATCGCGATCAGCGAGACAGGGTGGCCCACCGCCGGGGGCCCCGATGAGGTCGGGGCTTCCGTAAGCAACGCTGCTCTGTACAATGGAAGGCTAGTTAAGAAAATGATGTCGAATCCGCCCAAGGGTACCCCGAGAAGGCCCGGAGTTGGCgTACCCACCTTCATTTTTGCTCTCTTCAATGAAAACCAGAAGCCTGGGCCTGTGACCGAGAGAAACTGGGGGCTTCTCTATCCCAATGCTTCTCACGTTTATCCCCTGAATTTTTGA___GCTCTGTAATTAGGTTCCCATACTCTGCCATAATCTGTAATTAGGTTCCCATAGGCACTAATTTCCAGTTTCCATTCATTCATTTCGATTATTTTTTGAAATCTTTTGAGTAAATCGTGATTGAATTTGTACGCCAGATTCTGTGGCCTAGGCAGTGAAATTGTATATCAAATTCTGTGGCTCAAGATTGTTATGCTTGGCCAGCTTGTTTCAAATGTAATAATGGATATCACTAGTGAAACTCCTCAACTTCAATTGTCATGAACTACATTGAACTTCCATGAAACTGAATAAAATCACTGaTAAGCAGTGTCATTCCAAA

Fln Alignment:

AllPineV4_c13106___SMGINYGRLGDDLPPPEQAVALIKSLGFGQVKIFDSDPNILNALANTSLRVVMXXXXXXXXXXXXXXXXXXXXLDQQVRPHLPAARIRMITVGNELLSHPEINQPRWPLLLPAMQNLQEALxxxxxxxxIKVSTCIAMDALNVSYPPSAARFRADIADSVLRPVLQLLSSTKSYIFLNAYPYLAWSDDNNDIPLDYALFGPGATVVADGD-LQYGDLLDAQVDSFVAAMEDIGLPGVKIAISETGWPTAGGPDEVGASVSNAALYNGRLVKKMMSNPPKGTPRRPGVGVPTFIFALFNENQKPGPVTERNWGLLYPNASHVYPLN
D5AAN5_______________TIGVDYGQLGDNLPSTQEAIHLIQRMKAGRVKIYDTNPEILKALSHTGIKVSVMVKNEDIANVSSNQSFANKWVKNNVVYFYPATRINIILVGNEILSDYSNNQ-TWYQLVPAMLRIRRALxxxxxxxxIKVGTPLAMDVLNTSFPPSSGIFRDDVAETVMKPMLEFLSRTRSYFFIDVYPYFAWSSDSNNISLEYANFGDHDKNYTDPNGLVYTNLLDQQLDAVIAAMSKLGYEDIRLVIAETGWPNAGDLNQLGANIFNAAHYNRRIIRRMLADPPLGTPRRPNQFIPTYIFSLFNENQKTGLGTERHWGLLYPNGSRLYEID

SNPs (tot: 60)

Position	A %	C %	G %	T %	Probability
785			85	14	0.993533
786			14	85	0.993533
795	14		85		0.993533
797		14		85	0.993533
798		14	85		0.993533
799	14		85		0.993533
803	85			14	0.993533
808	85		14		0.993533
809	71		28		1.0
811		14		85	0.993533
861	16	83			0.994512
863			83	16	0.994512
864		83	16		0.994512
865	16		83		0.994512
866		16	83		0.994512
869		20	80		0.995493
870		20	80		0.995493
871	20	80			0.995493
872	20		80		0.995493
878		25		75	0.996473
895			66	33	0.997476
898	25			75	0.996473
900		75	25		0.996473
902		25		75	0.996473
1115	40	60			1.0
1135	20		80		0.995493
1145			40	60	1.0
1198		50		50	1.0
1202	50		50		1.0
1248		80	20		0.995493
1251		80		20	0.995493
1253	20	80			0.995493
1257			20	80	0.995493
1258		20	80		0.995493
1260	20			80	0.995493
1261	20			80	0.995493
1263		80	20		0.995493
1269	20		80		0.995493
1288		33		33	0.997753
1299	75			25	0.996473
1302			25	75	0.996473
1304			25	75	0.996473
1306		25		75	0.996473
1307	25	75			0.996473
1308		75	25		0.996473
1312	25			75	0.996473
1314		75	25		0.996473
1316		25		75	0.996473
1317	25		75		0.996473
1318		25	75		0.996473
1319		75	25		0.996473
1322		75	25		0.996473
1332		33		66	0.997452
1439		33	66		0.997452
1441	33			66	0.997452
1442			33	66	0.997452
1443			33	66	0.997452
1445		33	66		0.997452
1446	33	66			0.997452
1447		33		66	0.997452

SSRs (tot: 1)

Start position	End position	Sequence	Length
1352	1367	AGC AGC AGC AGC AGC A	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain