SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene30588

Length: 1518 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene30588

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative NPK1-related protein kinase [Arabidopsis thaliana] emb\|CAB79544.1\| putative NPK1-related protein kinase [Arabidopsis thaliana]	-	-	39%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	40%
Blast2go	protein	-	-	64%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	64%

Source	GOs	Term	Type	Identity
Blast2go	protein autophosphorylation	GO:0046777	Biological Process	64%
Blast2go	protein binding	GO:0005515	Molecular Function	64%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	64%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Phospholipase C, phosphatidylinositol-specific , X domain	IPR000909	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR015748	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Transcription regulator, HTH DeoR-type, conserved site	IPR018356	-	-
Sma3	Carbohydrate kinase, FGGY, conserved site	IPR018483	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LR19

Fln msg: STOP codon was not found. Distance to subject end: 6 aas, your sequence is shorter than subject: 505 - 902

Fln protein: KKQTILLHCVLNWVRMGIELEMGRTQERGWVRGAIIGAGASGTVSLAMSSSNGELFAVKSAAGSNVALDNEYQILRLLDSPYIVRCLGCGYSVENGAEVHNLFMEYMPGGSVADLLIKFGGRLDESVIRAYTRGILHGIDYLHRQGIVHCDIKGKNVLVGVNGVKLADFGSAKRIGDEGKGQEALRLRGTPQWMAPEVVNQVEQGPASDIWSLGCTVLEMVTGKPPWSHVSSTLAAMYRIGCTEELPELPASLSLHLRDFLEKCFRRDPKRRWTSAELLNHPFLNEHCSVIEAGEALKCPKSPISHLDFRNQVWDSYSSEITNIPSLNISSPIGEFSGELNRQVKQCPRPSPRDRMMELAAACKFEDVAHSPNWITSPHGQWIVVKSSKRNSTASVSSATSSKSLSKSDISNDFSIQGLPFAKEKCSASLETVNWEELQPRCELNECLEAVVSEHQSFSSSSAETLHHDLFSMTAASNLIEEGGDKYENSSQSVACGHLGNLNIP

Protein Length: 506

Fln nts: AAAAAAACAGACAATTCTTCTTCACTGTGTATTGAATTGGGTGCGAATGGGGATTGAACTCGAGATGGGGAGAACACAGGAAAGGGGATGGGTGAGAGGCGCCATTATTGGGGCCGGAGCTTCTGGGACAGTTAGCCTTGCTATGAGCAGCTCTAATGGCGAACTCTTTGCAGTCAAATCCGCAGCCGGTTCTAATGTCGCTTTGGATAATGAATACCAGATCTTGCGGCTTCTGGATTCTCCATATATTGTTCGCTGCCTGGGGTGCGGTTACAGCGTTGAGAACGGTGCAGAGGTGCACAATTTGTTCATGGAGTACATGCCGGGCGGCAGTGTAGCGGATCTTCTGATTAAATTTGGTGGGCGCCTGGACGAATCCGTGATCAGGGCTTACACTCGTGGCATCCTACATGGAATTGATTACCTCCACAGGCAAGGGATTGTGCACTGTGATATCAAGGGGAAAAATGTTCTTGTGGGTGTCAATGGTGTGAAGCTTGCGGATTTTGGTTCTGCCAAGAGGATTGGTGATGAGGGTAAGGGCCAGGAAGCCCTGCGATTGAGGGGGACACCTCAATGGATGGCTCCAGAGGTGGTGAATCAGGTGGAGCAGGGGCCGGCTTCCGATATTTGGTCTCTTGGCTGCACAGTGCTTGAGATGGTCACTGGCAAGCCTCCATGGAGCCATGTTTCCAGTACCCTCGCTGCAATGTATCGGATTGGATGCACAGAGGAGCTGCCTGAGTTGCCTGCATCGCTTTCACTTCATCTCCGGGATTTCTTAGAGAAATGCTTTCGTAGGGATCCGAAGAGGAGGTGGACCAGTGCAGAGCTGTTGAACCATCCTTTCCTAAATGAGCACTGCTCTGTCATAGAGGCAGGGGAAGCCCTTAAATGTCCGAAATCCCCAATTAGTCATTTGGATTTCAGGAATCAAGTATGGGATTCCTACTCTTCTGAGATTACTAACATTCCTTCACTCAATATCTCCAGCCCAATTGGAGAATTCAGTGGGGAGTTGAATAGACAAGTTAAGCAATGCCCAAGGCCTTCTCCCAGGGACAGAATGATGGAACTGGCCGCAGCTTGTAAATTTGAAGATGTTGCTCACAGCCCTAATTGGATCACAAGCCCTCATGGTCAGTGGATTGTTGTGAAATCTTCGAAAAGGAACTCGACAGCTTCAGTTTCCTCTGCAACTTCAAGCAAATCTCTGTCAAAATCAGATATCAGTAATGACTTTTCCATCCAAGGGCTTCCATTCGCAAAAGAAAAGTGTAGTGCCAGCTTGGAAACTGTCAATTGGGAAGAGTTGCAGCCCAGATGTGAACTAAATGAATGCTTGGAGGCTGTGGTCTCTGAACATCAATCATTTTCCAGTTCTTCTGCTGAGACTCTGCATCATGACTTGTTTTCAATGACGGCGGCATCCAATTTGATTGAAGAAGGCGGTGACAAGTATGAAAATTCATCCCAGAGTGTTGCTTGTGGCCATCTTGGAAATCTTAACATACCAGT

Fln Alignment:

AllPineV4_c12929___WVRGAIIGAGASGTVSLAMSSSNGELFAVKS---------AAGSNVALDNEYQILRLLDSPYIVRCLGCGYSVENGAEVHNLFMEYMPGGSVADLLIKFGGRLDESVIRAYTRGILHGIDYLHRQGIVHCDIXXXXXXXXXXXXXX-ADFGSAKRIGDEGKGQEALRLRGTPQWMAPEVVNQVEQGP--ASDIWSLGCTVLEMVTGKPPWSHVSSTLAAMYRIGCTXXXXXXXXXXXXXXRDFLEKCFRRDPKRRWTSAELLNHPFL
B8LR19_______________WQKGKLLGCGTFGTVYVGFNRETGDMCAMKEVPLVPDDSKSSESIKQLEQEINLLSGLEHPNIVQY----YGSETVEDLFYIYLEYVPGGSIYKLVNDYGP-LEEPVIRIYTRQILSGLAYLHSMNTVHRDIKGGNILVDTYGRVKLADFGMAKHINGPAT---PLSLKGSPYWMAPEVLMQKNTGHDLAVDIWSLGCTVIEMATGKPPWSEYEGA-AAMFKV-FKSEVPPIPDSLSPEGRHFVQCCLCRNPAQRPKASQLLEHLFV

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
247		50		50	1.0
269	25		75		0.996473
270		50	50		1.0
291		50		50	0.999893
292	50		50		0.999786
305		33		66	0.999956
325	33		66		0.999721
328		33		66	0.999978
335			33	66	0.999994
346			33	66	0.999993
359	33		66		0.997452
361	33			66	0.997452
385		60		40	1.0
484		28		71	1.0
485		71		28	1.0
537		25	75		1.0
539	14		85		0.993533
601	40		60		1.0
625			80	20	0.995496
726	16		83		0.994512
917		11		88	0.991528
954		22	77		0.999999
1085			70	30	1.0
1166		12		87	0.992554
1245	80	20			0.999996
1248	80	20			0.999996
1249	80		20		0.999996
1257			28	71	1.0
1258			28	71	1.0
1260		71		28	1.0
1261	71		28		1.0
1266	71			28	1.0
1267	71	28			1.0
1268	28		71		1.0
1419	57		42		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain