SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene30536

Length: 1961 nt

Origin of reads: Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene30536
CCGATGGTACGCATACTTCGTCTACTGTCGCCGGGTCGGAGGTGCTTGGTCGAGTCTGCTTGGCTTCGCCCAAGGAACGGCTAGAGGCATGGCCACCAAAGCCAGATTGGCCGTCTACAAGGTCTGCTGGGCAGAGGGTTGTGCCGACTCCGACCTGCTCGCAGGGATGGAGAAGGCAGTTTCCGACGGAGTGGACGTGATATCTGTATCCATTGGCAGCCGTAGCAATCATGTCCTCCCTTATTACGAAGATTCTATAGCGATGGGAGCTCTTGGAGCCATTGAGAAGGGTATTTTTGTGTCCTGTTCCGCTGGTAATAGAGGGCCGATTCCTTCTGCAATTTCCAATACGGCACCATGGATAACCACCGTAGGCGCCAGCACCATCGACAGAGAGTTCCCGGCTCCAGTTGTTCTGGGGAATGACAAAAACTACACAGGCGCGTCCCTTTACAAAGGCAAGACGGCGGGCAGCAAACAATTGCCGTTGGTATACGGGAAAACCGCGTCGACCACTGCTGAGACTGCAAATCTCTGTCTGGAGGGAAGCCTTGATCCCACTATGGTTCGCGGAAAAATTGTGGTCTGTGATCTTGGAGAAGAGGCAAGGGCGGAGAAGGGACTGGTCGTGCGACGAGCTGGCGGTGCGGGGATGATTCTGACCAACGGGCCCGATGAAGGGGAGGATTTGTCGACCGACTCTCATGTGTTACCCGCAACGAAGGTCAGTTTCAAATCGGCAGAAGACATTAAAGCGTATATCAACAGCACCAAAAATCCGACAGCATCAATTGAAGCCGATGGTCTCACTGTGGTGGGTAAAGCACGAGCTCCGGTTGTAGCTAGCTTCTCGTCCAGAGGGCCGAATCTGGTCGCTCCGGAAGTCCTTAAACCGGACATGGTTGCTCCGGGCGTCAACATATTGGCAGCGTGGACTGGTGACGCCGCTCCAACCGACTTGCCTTCTGATAAGAGAAGAGTAGATTACAACATCATATCTGGGACTTCGATGTCATGCCCTCATGTCACCGGCATAGCGGCTCTCATACGCGCTGTGCGTCCGACATGGACACCCGCTGCAATAAAATCGGCTCTCATGACATCAACCGTGCTACTTGATGATACTGAACGCCATATCATCGATGCAGTCACCGGGCGACCAGCAAACGCCTTAGCAGTAGGTGCGGGTCATGTGAATCCCAGTGCTGCCTTGGATCCAGGTCTTGTTTACGATCTGGAACTTGACGATCACTCATGTTTCTGTGTAGCCTAAATTACACCACAAAGCAAATTCATCTTCTAACAAGGAAGGCGAAGGCGAGCTCCTGCCCTAGGGTACCAGCACATCCCGTTGATCTCAACTACCCATCATTCTCCGTGGTGTTTAAACCGCCTAATTTAGTTCGCGTTACAACCAGAACTGTGACGAACGTGGGGGCAGCCCCCTCTGTGTATAAGATCGCAGTTGACAGCCCTTCAAGCGTAAACATAATCGTGGAGCCTAGCACACTGACCTTTCGGAAGAAAAATGAGAAGGCGAATTTTACAGTGAGATTCGAAAGCAAAATAGCATTCGGTCACAAATCTAGCGGACGCCAAAGGTTCGGGCAAATTTCATGGAAATGCATCAAGGGTGGAACGCAGGTTGTCCGAAGTCCGGTAGCCATAACCTGGACAAATGATGGTGAAACAACCGTCTCAGAAGCACATAAAAATTCTATTAGTCCATAATTGTATGGGATGCCTCCAATATACACAGTTCTTCTCTTTTTAATAAAAACTGATATGAGCAATCCTTCAAGATGCTGCACACTGTGTTAGATTTAGTTGGTTAATGGATTCAAACCCCTCGATGCATGTTTCTAATGTTTCTAGCTGCTGGAGCATATATGTTGATCAATCTTGCTCCAGCAGCTCATATGTTGATCAATCTTGCTCAGCAAGCAGCTCATATGTTGATC

Ace file of the UniGene sp_v2.0_unigene30536 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	subtilisin-like protease [Arabidopsis thaliana] sp\|O65351.1\|SUBL_ARATH RecName: Full=Subtilisin-like protease; AltName: Full=Cucumisin-like serine protease; Flags: Precursor gb\|AAK25995.1\|AF360285_1 putative subtilisin serine protease ARA12 [Arabidopsis t	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	80%
Blast2go	subtilisin-like protease	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on peptide bonds (peptide hydrolases), Serine endopeptidases.	EC:3.4.21.-	-	0.0	72%

Source	GOs	Term	Type	Identity
Blast2go	mucilage metabolic process involved seed coat development	GO:0048359	Biological Process	72%
Blast2go	mucilage extrusion from seed coat	GO:0080001	Biological Process	72%
Blast2go	serine-type endopeptidase activity	GO:0004252	Molecular Function	72%
Blast2go	plant-type cell wall	GO:0009505	Cellular Component	72%
Blast2go	apoplast	GO:0048046	Cellular Component	72%

Source	InterPros	Term	Type	Identity
Sma3	C2 calcium-dependent membrane targeting	IPR000008	-	-
Sma3	Phosphofructokinase domain	IPR000023	-	-
Sma3	Ornithine/DAP/Arg decarboxylase	IPR000183	-	-
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3		IPR000991	-	-
Sma3	Arf GTPase activating protein	IPR001164	-	-
Sma3	GMP synthase, C-terminal	IPR001674	-	-
Sma3	Protease-associated domain, PA	IPR003137	-	-
Sma3	GMP synthase, N-terminal	IPR004739	-	-
Sma3	Domain of unknown function DUF591	IPR007649	-	-
Sma3	Proteinase inhibitor I9	IPR010259	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3		IPR011702	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Peroxisome membrane protein, Pex16	IPR013919	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Peptidase S8, subtilisin-related	IPR015500	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Glutamine amidotransferase type 1	IPR017926	-	-
Sma3	C2 membrane targeting protein	IPR018029	-	-
Sma3	Chromogranin, conserved site	IPR018054	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-
Sma3	Peptidase S10, serine carboxypeptidase, active site	IPR018202	-	-
Sma3		IPR018318	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQY5

Fln msg: Overlapping hits, possible frame ERROR between 50 and 50, and overlapping frame ERROR between 1261 and 1258, your sequence is shorter than subject: 577 - 783

Fln protein: DGTHTSSTVAGSEVLxxSLLGFAQGTARGMATKARLAVYKVCWAEGCADSDLLAGMEKAVSDGVDVISVSIGSRSNHVLPYYEDSIAMGALGAIEKGIFVSCSAGNRGPIPSAISNTAPWITTVGASTIDREFPAPVVLGNDKNYTGASLYKGKTAGSKQLPLVYGKTASTTAETANLCLEGSLDPTMVRGKIVVCDLGEEARAEKGLVVRRAGGAGMILTNGPDEGEDLSTDSHVLPATKVSFKSAEDIKAYINSTKNPTASIEADGLTVVGKARAPVVASFSSRGPNLVAPEVLKPDMVAPGVNILAAWTGDAAPTDLPSDKRRVDYNIISGTSMSCPHVTGIAALIRAVRPTWTPAAIKSALMTSTVLLDDTERHIIDAVTGRPANALAVGAGHVNPSAALDPGLVYDLELDDHxxxxCSLNYTTKQIHLLTRKAKASSCPRVPAHPVDLNYPSFSVVFKPPNLVRVTTRTVTNVGAAPSVYKIAVDSPSSVNIIVEPSTLTFRKKNEKANFTVRFESKIAFGHKSSGRQRFGQISWKCIKGGTQVVRSPVAITWTNDGETTVSEAHKNSISP*

Protein Length: 578

Fln nts: CCGATGGTACGCATACTTCGTCTACTGTCGCCGGGTCGGAGGTGCTTxxxxxxAGTCTGCTTGGCTTCGCCCAAGGAACGGCTAGAGGCATGGCCACCAAAGCCAGATTGGCCGTCTACAAGGTCTGCTGGGCAGAGGGTTGTGCCGACTCCGACCTGCTCGCAGGGATGGAGAAGGCAGTTTCCGACGGAGTGGACGTGATATCTGTATCCATTGGCAGCCGTAGCAATCATGTCCTCCCTTATTACGAAGATTCTATAGCGATGGGAGCTCTTGGAGCCATTGAGAAGGGTATTTTTGTGTCCTGTTCCGCTGGTAATAGAGGGCCGATTCCTTCTGCAATTTCCAATACGGCACCATGGATAACCACCGTAGGCGCCAGCACCATCGACAGAGAGTTCCCGGCTCCAGTTGTTCTGGGGAATGACAAAAACTACACAGGCGCGTCCCTTTACAAAGGCAAGACGGCGGGCAGCAAACAATTGCCGTTGGTATACGGGAAAACCGCGTCGACCACTGCTGAGACTGCAAATCTCTGTCTGGAGGGAAGCCTTGATCCCACTATGGTTCGCGGAAAAATTGTGGTCTGTGATCTTGGAGAAGAGGCAAGGGCGGAGAAGGGACTGGTCGTGCGACGAGCTGGCGGTGCGGGGATGATTCTGACCAACGGGCCCGATGAAGGGGAGGATTTGTCGACCGACTCTCATGTGTTACCCGCAACGAAGGTCAGTTTCAAATCGGCAGAAGACATTAAAGCGTATATCAACAGCACCAAAAATCCGACAGCATCAATTGAAGCCGATGGTCTCACTGTGGTGGGTAAAGCACGAGCTCCGGTTGTAGCTAGCTTCTCGTCCAGAGGGCCGAATCTGGTCGCTCCGGAAGTCCTTAAACCGGACATGGTTGCTCCGGGCGTCAACATATTGGCAGCGTGGACTGGTGACGCCGCTCCAACCGACTTGCCTTCTGATAAGAGAAGAGTAGATTACAACATCATATCTGGGACTTCGATGTCATGCCCTCATGTCACCGGCATAGCGGCTCTCATACGCGCTGTGCGTCCGACATGGACACCCGCTGCAATAAAATCGGCTCTCATGACATCAACCGTGCTACTTGATGATACTGAACGCCATATCATCGATGCAGTCACCGGGCGACCAGCAAACGCCTTAGCAGTAGGTGCGGGTCATGTGAATCCCAGTGCTGCCTTGGATCCAGGTCTTGTTTACGATCTGGAACTTGACGATCACTCxxxxxxxxxGTGTAGCCTAAATTACACCACAAAGCAAATTCATCTTCTAACAAGGAAGGCGAAGGCGAGCTCCTGCCCTAGGGTACCAGCACATCCCGTTGATCTCAACTACCCATCATTCTCCGTGGTGTTTAAACCGCCTAATTTAGTTCGCGTTACAACCAGAACTGTGACGAACGTGGGGGCAGCCCCCTCTGTGTATAAGATCGCAGTTGACAGCCCTTCAAGCGTAAACATAATCGTGGAGCCTAGCACACTGACCTTTCGGAAGAAAAATGAGAAGGCGAATTTTACAGTGAGATTCGAAAGCAAAATAGCATTCGGTCACAAATCTAGCGGACGCCAAAGGTTCGGGCAAATTTCATGGAAATGCATCAAGGGTGGAACGCAGGTTGTCCGAAGTCCGGTAGCCATAACCTGGACAAATGATGGTGAAACAACCGTCTCAGAAGCACATAAAAATTCTATTAGTCCATAATTGTATGGGATGCCTCCAATATACACAGTTCTTCTCTTTTTAATAAAAACTGATATGAGCAATCCTTCAAGATGCTGCACACTGTGTTAGATTTAGTTGGTTAATGGATTCAAACCCCTCGATGCATGTTTCTAATGTTTCTAGCTGCTGGAGCATATATGTTGATCAATCTTGCTCCAGCAGCTCATATGTTGATCAATCTTGCTCAGCAAGCAGCTCATATGTTGATC

Fln Alignment:

AllPineV4_c12748___GTHTSSTVAGSEVLGSSLLGFAQGTARGMATKARLAVYKVCWAEGCADSDLLAGMEKAXXXXXXXXXXXXXSRSNHVLPYYEDSIAMGALGAIEKGIFVSCSAGNRGPIPSAISNTAPWITTVGASTIDREFPAPVVLGNDKNYTGASLYKGKTAGSKQLPLVYGKTASTTAETANLCLEGSLDPTMVRGKIVVCDLGE-EARAEKGLVVRRAGGAGMILTNGPDEGEDLSTDSHVLPATKVSFKSAEDIKAYINSTKNPTASIEADGLTVVGKARAPVVASFSSRGPNLVAPEVLKPDMVAPGVNILAAWTGDAAPTDLPSDKRRVDYNIISGTSMSCPHVTGIAALIRAVRPTWTPAAIKSALMTSTVLLDDTERHIIDAVTGRPANALAVGAGHVNPSAALDPGLVYDLELDDHSCxLCSLNYTTKQIHLLTRKAKASSCPRVPAH-PVDLNYPSFSVVFKPPNLXXXXXXXXXXVGAAPSVYKIAVDSPSSVNIIVEPSTLTFRKKNEKANFTVRFESKIAFGHKSSGRQRFGQISWKCIKGGTQVVRSPVAITW
B8LQY5_______________GTHTSSTAAGAEVPGSSLLGFAAGTARGIATKARLAVYKVCWPEECLSSDLLAGMEAAISDGVDLLSLSISDSRN--LPYYKDAIAIGALGAIEKGVFVSCAAGNAGPIPSKIFNTAPWITTVGASTIDREFPAPVVLGNGKNYRGSSLYKGKTLGNGQLPLIYGKSASSN-ETAKFCLAGSLDSNRVSGKIVLCDLGGGEGTAEMGLVVRQAGGAGMIQANRLVDGEDLWTDCHFLPATKVDFKSGIEIKAYINRTKNPTATIKAEGATVVGKTRAPVVASFSSRGPNPLVPEILKPDLIAPGVNVLAAWSGHVSPTGLTSDKRRVDYNIISGTSMACPHVTGIAALILAVHSAWTPAAIKSALMTSSVPFDHSKRLISESVTALPADAFAIGAGHVNPSAALDPGLVYDADFDDYVSxLCSLNYTRSQIHILTRKA--SSCTRIHSQQPGDLNYPSFSVVFKPLNLVRALRRTVTNVGGAPCVYEVSMESPPGVNIIVEPRTLVFKEQNEKASYTVRFESKTASHNKSSGRQEFGQIWWKCVKGGTQVVRSPVAIVW

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
1477		20		80	0.995493
1483	20	80			0.995493
1492		33		66	1.0
1494		33		66	1.0
1496		66		33	1.0
1504		66		33	1.0
1525		66	33		1.0
1527	33	66			1.0
1538		33		66	1.0
1544		33		66	1.0
1546	66		33		1.0
1558	66		33		1.0
1561			33	66	1.0
1594	40		60		1.0
1678	66		33		0.997458

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain