SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene29835

Length: 1913 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene29835 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pfam03145, Sina, Seven in absentia protein family	-	-	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	76%
Blast2go	ubiquitin ligase	-	-	92%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3	Zinc finger, RING-type	IPR001841	-	-
Sma3	Seven-in-absentia protein, sina	IPR004162	-	-
Sma3	Zinc finger, SIAH-type	IPR013010	-	-
Sma3	TRAF-type	IPR013322	-	-
Sma3	Seven In Absentia Homolog-type	IPR013323	-	-
Sma3	Aldehyde dehydrogenase domain	IPR015590	-	-
Sma3	Zinc finger, C2H2-like	IPR015880	-	-
Sma3	Aldehyde dehydrogenase, N-terminal	IPR016162	-	-
Sma3	Zinc finger, RING-type, conserved site	IPR017907	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-
Sma3	Seven-in-absentia protein, TRAF-like domain	IPR018121	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NX30

Fln msg: Separated hits, possible frame ERROR between 789 and 801,

Fln protein: MSCLRARKEQLGDGAPLVVPGLTYMDETGSPFTDIDSPPGPINEQPYVKDAYPPKNGKPGTLPKSIVHELLECPVCTNFMYPPIHQCPNGHTLCSHCKSKVHNKCPTCRQELGNIRHLTLxxxxxTLDLPCKYHNLGCPDIFPYYSKLKHEAQCTFRPYNCPYAGSECPITGDLQTLVAHLKDDHKVDMHNGSSFNHRYVKANPHEVENATWMLTIFHCYGAYFCLHFEAFQLGKSPVYIAFLRFMGDDNEAKNFSYSLEVGANGRKLIWQGVPRSIRDSHCKVRDSYDGLIIQRSMALFFSGGDMKELKLKVTGRIWKEQ*

Protein Length: 323

Fln nts: CCTAGTCAATCCACAATAACAAACAGCATTTTGCAAACCCCCATTTACGAAAGATCGCATGGGAAACTGCAAAAATCTTCAATAAAGAAATGGCTTGCTCTGTTTCGATGAAAGACCATCATTAGATCGATTCTCGAGGTCAACGTGCTGATCTGTACAGACTTGTCAATTATTGAATGCAGACTTCTCGTGCTGCGATCCACTGCTATCTCTAAAAACTTCTGTTTCATTTGAGCCAGAGTTCCACGCAAATTGCTGGAATTTTTGTTGCAGAGAGAATTGTAATAAGTCCTTATCAAAGCTTCACCTGGTACGTTTGAAGCAGCCAAGAAGTTGATACCTGCAAAATATTGTTGATTAAGTACAGTGAAGATAACCATTGTGTGGAGGACAAATCTTAACCAGATCAAGAAGTGGAAGATCTAT_-_ATGAGCTGTCTTAGAGCTAGAAAAGAACAACTAGGAGATGGAGCTCCTCTCGTAGTACCAGGTCTTACCTATATGGATGAAACAGGGTCACCGTTTACAGATATTGATTCTCCTCCAGGGCCAATAAATGAGCAACCTTATGTCAAAGATGCTTATCCACCCAAAAATGGAAAGCCTGGAACATTGCCAAAAAGTATTGTGCATGAACTGCTTGAGTGTCCTGTCTGCACAAATTTTATGTATCCACCAATTCATCAGTGTCCTAATGGGCATACCCTGTGCTCCCACTGTAAGTCAAAGGTGCATAACAAGTGCCCCACATGCAGACAGGAGCTTGGAAATATAAGACACCTAACATTGGAxxxxxxxxxxxxCACTCTTGATCTGCCTTGCAAGTATCATAATCTTGGTTGCCCAGATATTTTCCCATATTATAGCAAGCTTAAACATGAGGCCCAATGTACTTTCAGGCCATACAATTGCCCTTATGCAGGTTCCGAATGTCCTATCACAGGGGATCTTCAGACACTTGTGGCACATTTAAAGGATGATCACAAAGTTGATATGCACAATGGTAGCTCGTTCAACCACCGTTATGTGAAGGCTAATCCACATGAAGTGGAGAATGCAACCTGGATGCTGACGATTTTCCACTGTTATGGAGCATATTTTTGTCTTCATTTTGAGGCATTTCAGTTAGGCAAGTCACCAGTGTATATTGCTTTCCTTCGCTTTATGGGAGATGATAATGAAGCCAAGAATTTCAGCTATAGTCTTGAAGTAGGGGCGAATGGCAGGAAGCTAATATGGCAAGGAGTGCCTAGGAGCATCCGGGACAGCCATTGTAAAGTTCGTGACAGCTATGACGGGCTAATCATACAAAGAAGCATGGCATTGTTCTTTTCTGGTGGAGACATGAAAGAATTGAAGTTGAAAGTTACTGGACGAATCTGGAAGGAACAATGA___GCTATGAAGTGGTGCTCGGAATTGCAACTTCCATACAACTGACTTGTGCTCAATGCCTGGTCGATGGAAGCCTCTGTTGAGAAGGTCGTGTTGGGTTGTGTTTTGTGTGAAGTTCATAGTCTAGTTGGACTGCAATAGTTCTGCCTAGGATATACAAGGTCATTCTTTATTTAGGAGAAAAGTGTATACCAATAAACATGTAACTTATATCATTTGTATGATTGtATCgTGtcATTGAGCGATtacTgATACCTGATCTGTTTAAAACAATCAAAGCTGTTTTCTGTCAGATGTTTTGAAGTTTGCAGTCATATTTGTTGGTGGTTTTGCATAATTTGTGGAGGCAATTGAGCAGTTCATGTCTTCTCTAATTGTCAGGAGGTAATGATGAGGTGATAAGTGAGAAGCAAGGTGTTTTACAACCTATGCGAGTTGAGTTTTTTGTGTCGTATATGTGTAATGCTAAAAAGATGTAGATCCATAGATCCATATTAATCCCAGATCTATTAAAAAAGTGAGAAG

Fln Alignment:

AllPineV4_c10633___NGKPGTLPK--SIVHELLECPVCTNFMYPPIHQCPNGHTLCSHCKSKVHNKCPTCRQELGNIRHLTLExxxxTLDLPCKYHNLGCPDIFPYYSKLKHEAQCTFRPYNCPYAGSECPITGDLQTLVAHLKDDHKVDMHNGSSFNHRYVKANPHEVENATWMLTIFHCYGAYFCLHFEAFQLGKSPVYIAFLRFMGDDNEAKNFSYSLEVGANGRKLIWQGVPRSIRDSHCKVRDSYDGLIIQRSMALFFSGGDMKELKLKVTGRIWKEQ
A9NX30_______________NSNNGTISPAASSVHELLECPVCTNSMYPPIHQCHNGHTLCSSCKSRVHNKCPTCRQELGDIRCLALExxxxSLELPCKHYNLGCPEIFPYYSKLKHEALCTFRPYNCPYAGSECSVTGDIQSLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNFSYSLEVGANGRKMIWQGVPRSIRDSHRKVRESHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
1444	80			20	0.995493
1507		66		33	0.997452
1810		20		80	0.995493
1811		20		80	0.995494
1812		20		80	0.995494
1844	75	25			0.996481
1845	75	25			0.996479
1846		25	75		0.996479
1880	66	33			0.997453
1881	66	33			0.997453

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain