SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene29817

Length: 2045 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene29817
CGTCGTGACCTTGGCTGTCACTCAAAGTTCTGTTAGTTACTTTTCCTAACTGACAATCTCCTGTATATCAATCAAACTGCAGCCTATTGAAAAATCTGTTTTCCTGTGTGTTTAGCAGACAAGAATTTGAAGTCTGTAGAAGGTATGGCCATGGAGAAACTATGGACGGGCTTGCTGTTACTGGCCTTGTTGCTTATGATGAGCTATGCAGGGGACGCAGGGCGTCCTTCCTTCTGGAGGCCCAAAACTCTGGGCGAGTTTTCAATTATTAACCGAGAGAAGTCATTTTTACAATCATCACAGTACGAGTACGAGACCAAGTATTTCACACAAAGGCTTGATCATTTTAGCTTCAAGAATTATGAGAATTCAACGTTCCAGCAGCGTTATCTCATAAATGACAAGTACTGGCTAGGGGCAGAACGTATGGGGCCTATCTTTCTTTATTGCGGGAATGAGGGCTACATTGACTGGTTTGCTGTGAACACTGGATTTATGTGGGACATAGCACCTCAGTTCGGAGCTCTGCTCGTCTTTCCAGAACACCGCTACTATGGAGAGTCTATGCCCTATGGCAATCAGAGTGTGGCCTACAAGGATGGAGATTCACTATCTTATCTCACAGCTGAGCAAGCACTGGCTGATTTTGCAACTTTGATAGTAGACCTAAAAAAGAACCTATCTGCCAAGGCTTGTCCAGTTGTCTTGTTTGGTGGTTCTTATGGTGGAATGTTAGCTGCTTGGATGAGGTTGAAATATCCACATATTGCAATAGGTGCCCTAGCTTCATCTGCTCCAATATTGCAGTTTGAAGACATTGTACCATCTGATACATTCTACAATCTTGTTTCCAATGATTTCAAGCGGGAAAGTGAGAACTGTTTCAAGGTGATACAGCAATCATGGAAAGCATTGGAGACATATGGTGAAAGAGATGAAGGGTTACAAAATCTTAGCAAGAAGTTTCGCATGTGCAGGGATCTCAAAAGCACAGAGGAAGTACAAAACTGGTTAAGCTCAGCATACAGTAACCTTGCTATGGTGGACTATCCATATCCAGCTAGTTTCTTAATGCCTTTGCCTGGCTTCCCAATAAGAGAGGTCTGCAAAGTGATTGATAGTTTTTCTAATGGCACGGATGTGTTGGACCAAATATTTGCGGGTGTTAGCGTTTACTACAACTATACAGGAGAAGAAAAATGTTTTGACGTCAATGATGATCCTCATGGCGAAAACGGGTGGAATTGGCAGGCTTGCACGGAGATGGTTATGCCTATGTCTAGCAATCCAGAGAACAGCATTTTCCCTCAGTTTACTTTCGACATGGACTCATACAAACAAGATTGTTTGAATATGTTTGGTGTAGAACCACGAGCTCACTGGATAACAACGGAATTTGGTGGTCATGACATACAGAAAGTATTGAAAAACTACGGTAGCAATATAATTTTCTCAAATGGTCTTTTGGACCCCTGGAGCGGGGGAGGTGTTCTTCAAAACATTTCGAGCAGCATTGTAGCACTCGTAACAGAGCTAGGTGCCCATCACTTGGATTTGCGTGCGGCAACGGAAAATGATCCAATTTGGCTAGTAGAGCAACGAAATGCGGAAATTCAAATTATAAATAATTGGATGAATGAATACTATCAAAACATAGGAATTTATACCTCTGAAATTTTGGGCAAGAATGAGTACTATCAAGACAAAGGAATTTATGCTTCTGAAATTTAGGGGCAAATATTGCCCTTCTGGTGGGCATGATTAATACCAACCAGTTTCTGTCTGTTTTGAGCCTGTTTGTGTTGCGTGTTGTAGAACATTTGGGTTAAATAGAAGACAAACACGTGCCAAACAGTTGGTGCCAGAAATTAGTGAAAACTGATCCTTACATCACAGTGTATCGAATGAGTTTGTGGATCAATTTCATTGACTTCCTGAAGTGGAGTTTGTGACTTGTAGAAATGAAAATTTCATTTTAACATTATTATTATTTTTGAAATTGTTTCCCATGCGACATTGAAAATCAATTCGCCCTTATATTAAGG

Ace file of the UniGene sp_v2.0_unigene29817 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Serine carboxypeptidase S28 family protein [Arabidopsis thaliana] gb\|ABF57288.1\| At5g65760 [Arabidopsis thaliana] dbj\|BAF00060.1\| lysosomal Pro-X carboxypeptidase [Arabidopsis thaliana] gb\|AED98102.1\| Serine carboxypeptidase S28 family protein [Arabidopsi	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	serine carboxypeptidase s28 family protein	-	-	78%

Source	GOs	Term	Type	e value	Identity
Blast2go	vacuole	GO:0005773	Cellular Component	0.0	78%
Blast2go	chloroplast	GO:0009507	Cellular Component	0.0	78%

Source	InterPros	Term	Type	e value	Identity
Sma3	Peptidase S28	IPR008758	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPX2

Fln msg:

Fln protein: MAMEKLWTGLLLLALLLMMSYAGDAGRPSFWRPKTLGEFSIINREKSFLQSSQYEYETKYFTQRLDHFSFKNYENSTFQQRYLINDKYWLGAERMGPIFLYCGNEGYIDWFAVNTGFMWDIAPQFGALLVFPEHRYYGESMPYGNQSVAYKDGDSLSYLTAEQALADFATLIVDLKKNLSAKACPVVLFGGSYGGMLAAWMRLKYPHIAIGALASSAPILQFEDIVPSDTFYNLVSNDFKRESENCFKVIQQSWKALETYGERDEGLQNLSKKFRMCRDLKSTEEVQNWLSSAYSNLAMVDYPYPASFLMPLPGFPIREVCKVIDSFSNGTDVLDQIFAGVSVYYNYTGEEKCFDVNDDPHGENGWNWQACTEMVMPMSSNPENSIFPQFTFDMDSYKQDCLNMFGVEPRAHWITTEFGGHDIQKVLKNYGSNIIFSNGLLDPWSGGGVLQNISSSIVALVTELGAHHLDLRAATENDPIWLVEQRNAEIQIINNWMNEYYQNIGIYTSEILGKNEYYQDKGIYASEI*

Protein Length: 530

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAAGTTCTGTTAGTTACTTTTCCTAACTGACAATCTCCTGTATATCAATCAAACTGCAGCCTATTGAAAAATCTGTTTTCCTGTGTGTTTAGCAGACAAGAATTTGAAGTCTGTAGAAGGT_-_ATGGCCATGGAGAAACTATGGACGGGCTTGCTGTTACTGGCCTTGTTGCTTATGATGAGCTATGCAGGGGACGCAGGGCGTCCTTCCTTCTGGAGGCCCAAAACTCTGGGCGAGTTTTCAATTATTAACCGAGAGAAGTCATTTTTACAATCATCACAGTACGAGTACGAGACCAAGTATTTCACACAAAGGCTTGATCATTTTAGCTTCAAGAATTATGAGAATTCAACGTTCCAGCAGCGTTATCTCATAAATGACAAGTACTGGCTAGGGGCAGAACGTATGGGGCCTATCTTTCTTTATTGCGGGAATGAGGGCTACATTGACTGGTTTGCTGTGAACACTGGATTTATGTGGGACATAGCACCTCAGTTCGGAGCTCTGCTCGTCTTTCCAGAACACCGCTACTATGGAGAGTCTATGCCCTATGGCAATCAGAGTGTGGCCTACAAGGATGGAGATTCACTATCTTATCTCACAGCTGAGCAAGCACTGGCTGATTTTGCAACTTTGATAGTAGACCTAAAAAAGAACCTATCTGCCAAGGCTTGTCCAGTTGTCTTGTTTGGTGGTTCTTATGGTGGAATGTTAGCTGCTTGGATGAGGTTGAAATATCCACATATTGCAATAGGTGCCCTAGCTTCATCTGCTCCAATATTGCAGTTTGAAGACATTGTACCATCTGATACATTCTACAATCTTGTTTCCAATGATTTCAAGCGGGAAAGTGAGAACTGTTTCAAGGTGATACAGCAATCATGGAAAGCATTGGAGACATATGGTGAAAGAGATGAAGGGTTACAAAATCTTAGCAAGAAGTTTCGCATGTGCAGGGATCTCAAAAGCACAGAGGAAGTACAAAACTGGTTAAGCTCAGCATACAGTAACCTTGCTATGGTGGACTATCCATATCCAGCTAGTTTCTTAATGCCTTTGCCTGGCTTCCCAATAAGAGAGGTCTGCAAAGTGATTGATAGTTTTTCTAATGGCACGGATGTGTTGGACCAAATATTTGCGGGTGTTAGCGTTTACTACAACTATACAGGAGAAGAAAAATGTTTTGACGTCAATGATGATCCTCATGGCGAAAACGGGTGGAATTGGCAGGCTTGCACGGAGATGGTTATGCCTATGTCTAGCAATCCAGAGAACAGCATTTTCCCTCAGTTTACTTTCGACATGGACTCATACAAACAAGATTGTTTGAATATGTTTGGTGTAGAACCACGAGCTCACTGGATAACAACGGAATTTGGTGGTCATGACATACAGAAAGTATTGAAAAACTACGGTAGCAATATAATTTTCTCAAATGGTCTTTTGGACCCCTGGAGCGGGGGAGGTGTTCTTCAAAACATTTCGAGCAGCATTGTAGCACTCGTAACAGAGCTAGGTGCCCATCACTTGGATTTGCGTGCGGCAACGGAAAATGATCCAATTTGGCTAGTAGAGCAACGAAATGCGGAAATTCAAATTATAAATAATTGGATGAATGAATACTATCAAAACATAGGAATTTATACCTCTGAAATTTTGGGCAAGAATGAGTACTATCAAGACAAAGGAATTTATGCTTCTGAAATTTAG___GGGCAAATATTGCCCTTCTGGTGGGCATGATTAATACCAACCAGTTTCTGTCTGTTTTGAGCCTGTTTGTGTTGCGTGTTGTAGAACATTTGGGTTAAATAGAAGACAAACACGTGCCAAACAGTTGGTGCCAGAAATTAGTGAAAACTGATCCTTACATCACAGTGTATCGAATGAGTTTGTGGATCAATTTCATTGACTTCCTGAAGTGGAGTTTGTGACTTGTAGAAATGAAAATTTCATTTTAACATTATTATTATTTTTGAAATTGTTTCCCATGCGACATTGAAAATCAATTCGCCCTTATATTAAGG

Fln Alignment:

AllPineV4_c10579___MEKLWTGXXXXXXXLMMSYAGDAGRPSFWRPKTLGEFSIINREKSFLQSSQYEYETKYFTQRLDHFSFKNYENSTFQQRYLINDKYWLGAERMGPIFLYCGNEGYIDWFAVNTGFMWDIAPQFGALLVFPEHRYYGESMPYGNQSVAYKDGDSLSYLTAEQALADFATLIVDLKKNLSAKACPVVLFGGSYGGMLAAWMRLKYPHIAIGALASSAPILQFEDIVPSDTFYNLVSNDFKRESENCFKVIQQSWKALETYGERDEGLQNLSKKFRMCRDLKSTEEVQNWLSSAYSNLAMVDYPYPASFLMPLPGFPIREVCKVIDSFSNGTDVLDQIFAGVSVYYNYTGEEKCFDVNDDPHGENGWNWQACTEMVMPMSSNPENSIFPQFTFDMDSYKQDCLNMFGVEPRAHWITTEFGGHDIQKVLKNYGSNIIFSNGLLDPWSGGGVLQNISSSIVALVTELGAHHLDLRAATENDPIWLVEQRNAEIQIINNWMNEYYQNIGIYTSEI
B8LPX2_______________MEQLWTGFLLLSLLPMMSCVADAGRPYFLRPRTLGEFSTLNREKSFLQSSQYEYETKYFTQRLDHFSFKNHKNSTFQQRYLINDKYWLGAERMGPIFYYCGNEGYIDWFAVNTGFMWDIAPQFGALLVFPEHRYYGESMPYGSQSMAYKDGDSLSYLTAEQALADFATLIVDLKKNLSAEACPVVLFGGSYGGMLAAWMRLKYPHIAIGALASSAPILQFEDIVPSDTFYNLVSNDFKRESENCFKVIQQSWKALETYGERDEGLQNLSKKFRMCRDLNSTDEIEDWLNSAYSNLAMVDYPYPASFLMPLPAYPIKEVCKVIDSFSNGTDVLDRIFAGVSVYYNYTGEEKCFDVNDDPHGENGWNWQACTEMVMPMSSNPESSIFPQFTFDIESYTKNCLNMFGVEPRPHWITTEFGGQDIKRVLKNYGSNIIFSNGLLDPWSGGGVLQNISNSIVALVTELGAHHLDLRAATENDPLWLVEQRNAEMKIINKWMNEYYQDKGIYTFEI

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
1223		16		83	0.999991
1226		83		16	0.999991
1403		10		90	0.90298
1672		72		27	1.0
1806		25	75		0.996476
1960	83	16			0.994512
1961		16	83		0.994512
1962	83	16			0.994512
1963		16		83	0.994513
1964	20			80	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
54	65	AAT AAT AAT AAT	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain