SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene29048

Length: 2114 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

>sp_v2.0_unigene29048
ATGGGGAAATAAATCGCATGGCAAAGTGTAGTATTATGGCTGCGATTTCTGAATCTGCTTCCGCCGAAATGAGCAATGAGATTTCCATCCCTCGACGAACGGCCAATCGCCACGGAAACGTGTGGGACGATGAGTTCATACTATCTCTCAACTCGCCCTATGGGGCACCGGCATATTATGAACGCGTTGGAATGCTTGTTGAGGAGATCAAGCAATTACTTTTGAGCGAAATGGAAGATAGCAATTATGATCTAATCAAACGTCTTGACATAGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACATGAAATAAAACAGGTGCCCTAGATTACGTTTACAGATGTTGGAACGAGAAGGGCATCGGAGTGGGATCGAGCGATTCCTGCACAGATCTTAACGCGACGGCTCTAGGATTTCGTGCTCTCCGACTGCATCGATATAACGTGTCTTCAGGTGTGATGGAGAATTTCAAGGGCGAAAACGCGAAGTTCGTCTGCAATTTTACTGGAGAGAAACGAGTGAGAAGCATGTTGTCGTTACTTCGAGCTTCAGACATTTCATTTCCCGGAGAAAAAGTGATGCAAGAGGCAAACGCTTTCACAAGAGAATATCTAAATCATGTTTTAGCTGGACGCGGGGATGTGACCGACGTAGATCAAAGTCTTTTGGAAGAGGTAAAGTACGCATTGGAGTTTCCCTGGTATTGCAGTGCGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATATATGGACAAAACAGCGCGTGGCTCAAGTCGAATTTCAACCAAAAAGTTCTGGAGTTAGCCAAATTGGACTTCAATATCCTGCAATGCATACATCAGAAAGAGTTACAGTTTATTACAAGGTGGTGGCGAGAGTCAGAAATTTCACAGCTGAATTTCTATAGGATACGTCACGTGGAATATCTTTTCTGGGCGGCTACATGCATTTTCGAGCCCGAATTCTCTCAAAGCAGAATTGCCTTGGCACAAGTTTGTGTGTTGGCAACTGTTCTAGATGACTTCTATGACACTCACGGTCTCTTGGACGAACTGAAAACCACTACGGAGGCAGTCAGACGATGGGATCTTTCACTGATAGATGACCTTCCAGACCACATAAAAATTGCATTCCGGTTTTTCTTCAAGACAACGAATGAATTGGCTGATCAAGTCCTCAAAGTGCAAAAGCGGGATATGGCAGCCACATTAAAGTGTAGTAGCTGGCAGCGCTATGTTGAGTCTTATTTGCAGGAAGGGGAATGGATAGCGACTGCTCATGTGCCCACGTTTAATGAGTACATAAAGAATGCGCGCGCTAGCTCAGGGATGTGTATTCTAAATTTGTTTCCCCTTCTCTTGATGGGTCAACTTCTCCCAAACAACGTTCTGGAGCAAATATATTCTCCGTCTAAGATGCAGGAGCTCTCAGAATTTACGGGCAGACTCATCGACGACTTGAAAGATTTCGAGGACGAGAAGGAACACGGGGAGATGGCTTCAAGTATAGCTTGTTATATTAAAGACAATCCTGATTGTACCGTGGAAAATGCGATAAATCACATCAAGGGCATCCTTCATCTCTCCCTTGAGGAATTGAATCGGGAGTATCTTAAGGAGGACAGTGTACCATTATGTTGCAAGAAGTTCAATTTCAATATAACACGAGGACTTCAATTTCTGTTCAGATACGGAGACGGCTTATCCGTATCTAACAAGGAAGTGAAGGACCAAATATTCAAAATTCTCGTCGATCAAGTGCTTATCAAGGAATAATGGCGACCCGCTTACTGTTACTAGGTGTGTGTATTAAGTATGTTACTGTTACTAGCTGTGTGTCTTAAGTATGTTTTGAATAAGTGGGGCTACTAAGAATAAGAGTTTTAGTGCAAATGTTTATGGACGTAGTTTAGCCAAGTTCTGCCAAAGTGCCCGTGGAAATTGTTCCCTGACAAATTCCAAGTATCGTGTACCGTAATTACAATCATCACGCAATCACCGCTGCACCATTTTCCACACCATTTTCCATTTTTCTACTGTATTCTTATATGTTTTATCATCCTGAAAGAAATATTATATTTTCCTTATAGTTAGGTTAAAAAGT

Ace file of the UniGene sp_v2.0_unigene29048

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Alpha-humulene synthase n=2 Tax=Picea RepID=F2XF97_PICGL	-	-	76%
FL-Next	tr=Delta-selinene-like synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Blast2go	a chain structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production	-	-	77%

Source	InterPros	Term	Type	Identity
Sma3	Terpene synthase-like	IPR001906	-	-
Sma3	Terpene synthase, metal-binding domain	IPR005630	-	-
Sma3	Terpenoid synthase	IPR008949	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: Q20HU6

Fln msg: Overlapping hits, possible frame ERROR between 326 and 323,

Fln protein: MAKCSIMAAISESASAEMSNEISIPRRTANRHGNVWDDEFILSLNSPYGAPAYYERVGMLVEEIKQLLLSEMEDSNYDLIKRLDIVDTLECLGIDRHFQHExxxGALDYVYRCWNEKGIGVGSSDSCTDLNATALGFRALRLHRYNVSSGVMENFKGENAKFVCNFTGEKRVRSMLSLLRASDISFPGEKVMQEANAFTREYLNHVLAGRGDVTDVDQSLLEEVKYALEFPWYCSAPRWEARSFIEIYGQNSAWLKSNFNQKVLELAKLDFNILQCIHQKELQFITRWWRESEISQLNFYRIRHVEYLFWAATCIFEPEFSQSRIALAQVCVLATVLDDFYDTHGLLDELKTTTEAVRRWDLSLIDDLPDHIKIAFRFFFKTTNELADQVLKVQKRDMAATLKCSSWQRYVESYLQEGEWIATAHVPTFNEYIKNARASSGMCILNLFPLLLMGQLLPNNVLEQIYSPSKMQELSEFTGRLIDDLKDFEDEKEHGEMASSIACYIKDNPDCTVENAINHIKGILHLSLEELNREYLKEDSVPLCCKKFNFNITRGLQFLFRYGDGLSVSNKEVKDQIFKILVDQVLIKE*

Protein Length: 591

Fln nts: ATGGGGAAATAAATCGC_-_ATGGCAAAGTGTAGTATTATGGCTGCGATTTCTGAATCTGCTTCCGCCGAAATGAGCAATGAGATTTCCATCCCTCGACGAACGGCCAATCGCCACGGAAACGTGTGGGACGATGAGTTCATACTATCTCTCAACTCGCCCTATGGGGCACCGGCATATTATGAACGCGTTGGAATGCTTGTTGAGGAGATCAAGCAATTACTTTTGAGCGAAATGGAAGATAGCAATTATGATCTAATCAAACGTCTTGACATAGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACATGAAxxxxxxxxxGGTGCCCTAGATTACGTTTACAGATGTTGGAACGAGAAGGGCATCGGAGTGGGATCGAGCGATTCCTGCACAGATCTTAACGCGACGGCTCTAGGATTTCGTGCTCTCCGACTGCATCGATATAACGTGTCTTCAGGTGTGATGGAGAATTTCAAGGGCGAAAACGCGAAGTTCGTCTGCAATTTTACTGGAGAGAAACGAGTGAGAAGCATGTTGTCGTTACTTCGAGCTTCAGACATTTCATTTCCCGGAGAAAAAGTGATGCAAGAGGCAAACGCTTTCACAAGAGAATATCTAAATCATGTTTTAGCTGGACGCGGGGATGTGACCGACGTAGATCAAAGTCTTTTGGAAGAGGTAAAGTACGCATTGGAGTTTCCCTGGTATTGCAGTGCGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATATATGGACAAAACAGCGCGTGGCTCAAGTCGAATTTCAACCAAAAAGTTCTGGAGTTAGCCAAATTGGACTTCAATATCCTGCAATGCATACATCAGAAAGAGTTACAGTTTATTACAAGGTGGTGGCGAGAGTCAGAAATTTCACAGCTGAATTTCTATAGGATACGTCACGTGGAATATCTTTTCTGGGCGGCTACATGCATTTTCGAGCCCGAATTCTCTCAAAGCAGAATTGCCTTGGCACAAGTTTGTGTGTTGGCAACTGTTCTAGATGACTTCTATGACACTCACGGTCTCTTGGACGAACTGAAAACCACTACGGAGGCAGTCAGACGATGGGATCTTTCACTGATAGATGACCTTCCAGACCACATAAAAATTGCATTCCGGTTTTTCTTCAAGACAACGAATGAATTGGCTGATCAAGTCCTCAAAGTGCAAAAGCGGGATATGGCAGCCACATTAAAGTGTAGTAGCTGGCAGCGCTATGTTGAGTCTTATTTGCAGGAAGGGGAATGGATAGCGACTGCTCATGTGCCCACGTTTAATGAGTACATAAAGAATGCGCGCGCTAGCTCAGGGATGTGTATTCTAAATTTGTTTCCCCTTCTCTTGATGGGTCAACTTCTCCCAAACAACGTTCTGGAGCAAATATATTCTCCGTCTAAGATGCAGGAGCTCTCAGAATTTACGGGCAGACTCATCGACGACTTGAAAGATTTCGAGGACGAGAAGGAACACGGGGAGATGGCTTCAAGTATAGCTTGTTATATTAAAGACAATCCTGATTGTACCGTGGAAAATGCGATAAATCACATCAAGGGCATCCTTCATCTCTCCCTTGAGGAATTGAATCGGGAGTATCTTAAGGAGGACAGTGTACCATTATGTTGCAAGAAGTTCAATTTCAATATAACACGAGGACTTCAATTTCTGTTCAGATACGGAGACGGCTTATCCGTATCTAACAAGGAAGTGAAGGACCAAATATTCAAAATTCTCGTCGATCAAGTGCTTATCAAGGAATAA___TGGCGACCCGCTTACTGTTACTAGGTGTGTGTATTAAGTATGTTACTGTTACTAGCTGTGTGTCTTAAGTATGTTTTGAATAAGTGGGGCTACTAAGAATAAGAGTTTTAGTGCAAATGTTTATGGACGTAGTTTAGCCAAGTTCTGCCAAAGTGCCCGTGGAAATTGTTCCCTGACAAATTCCAAGTATCGTGTACCGTAATTACAATCATCACGCAATCACCGCTGCACCATTTTCCACACCATTTTCCATTTTTCTACTGTATTCTTATATGTTTTATCATCCTGAAAGAAATATTATATTTTCCTTATAGTTAGGTTAAAAAGT

Fln Alignment:

AllPineV4_c7832___PRRTANRHGNVWDDEFILSLNSPYGAPAYYERVGMLVEEIKQLLLSEMEDSNYDLIKRLDIVDTLECLGIDRHFQHEIxTGALDYVYRCWNEKGIGVGSSDSCT-DLNATALGFRALRLHRYNVSSGVMENFKGENAKFVCNFTGEKRVRSMLSLLRASDISFPGEKVMQEANAFTREYLNHVLAGRGDVTDVDQSLLEEVKYALEFPWYCSAPRWEARSFIEIYGQNSAWLKSNFNQKVLELAKLDFNILQCIHQKELQFITRWWRESEISQLNFYRIRHVEYLFWAATCIFEPEFSQSRIALAQVCVLATVLDDFYDTHGLLDELKTTTEAVRRWDLSLIDDLPDHIKIAFRFFFKTTNELADQVLKVQKRDMAATLKCSSWQRYVESYLQEGEWIATAHVPTFNEYIKNARASSGMCIXXXXXXXXXXXXXXXXXXEQIYSPSKMQELSEFTGRLIDDLKDFEDEKEHGEMASSIACYIKDNPDCTVENAINHIKGILHLSLEELNREYLKEDSVPLCCKKFNFNITRGLQFLFRYGDGLSVSNKEVKDQIFKILVDQVLIKE
Q20HU6_______________PRRTANHHGNVWDDDLILSLDSPYGAPAYYERVANLIEEMKHLLLREMEDSNHDLIRRLQIVDTLECLGIDRHFQHEIxTAALDYVYRCWNEKGIGMGSSDSGSKDLDATALGLRALRLHRYNVSSGVLENFKDENGKFFCNLTGDKRVRSMLSLLRASEISFPGEKVMQEAKAFTREYLTQVLAGRGDVTDVDQSLLREVKYALEFPWHWSVPRWEARSFIEIYGQNHSWLKSNINQKVLELAKLDFNILQCIHQKEIQFIVRWWRESEIAQLNFYRKRHVEFYFWVVICIFEPEFSQSRIAFAKICTVATVLDDLYDTHGMLDELKTVTEGVSRWDLPLIDDLPDNIKIAFQFFFNTANELAVEVVKKQGRDMIALLK-ANWQRYVESYLQEAEWIATRHVPSFDEYIKNARASSGMCIGNLIPLLLLGQLLANNIVEQIHSPSKIQELSELTIRLIDDIRDFEDEKERGEIASAIECYMKDNPDSTVENALNHLEGILHLSLEELNWEFIKQDTVPLCCKKFTFNIVRGLQFVYKYGDGLSISNKEVKDQIFKILIDQVPIEE

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
16		66	33		0.997454
18		66	33		0.997454
223		12		87	0.992554
260		75		25	1.0
284	87		12		0.992554
286		12		87	0.992554
302		12		87	0.992554
627	25			75	0.996473
630		75	25		0.996473
879		20		80	0.995493
968	14		85		0.993533
1275		88		11	0.991528
1894			25	68	1.0
1995	14			85	0.993533
1996		71		28	1.0
2017		14		85	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain