SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28649

Length: 2338 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28649
AATGTATGGGGAGGCAATTACAATTATTCCTCTGAGAGGCCTGGAAATGGAGCTTGGAAGGGTAGAGCATCTTATATTGCGGATGGCCAGAAGCCTGGCCGCTGGGCGAACAACCCTCGTAGCGATAGAGACCGGGGTTTTAATCAACAGGGATGGAAGGAGTTGGGGAAACCTCATCAGAGGAATGTAAATCAAGAAAATAGGACCGCCAGAGCTAGAAATGCTAGCGGGTGGGGCCATTATGGCAGGGCAAATCAAGCAACACAGAGCAGCTGGAAGCCCGAAAACTCAGAGTCTGCCTATGGAAAGAAGGGCAAGGCAGATCTTTCTGTTCCTCCCCCACTTGCTAACGGTTGGCAATGGCGCAGCGACGCCAAGTTGGATTCTTCAATGGCATCATTACCCCGTGGAAAAGGTTCCGGACACAGGAGTGCAGATGCTAGTGAAGAGAAATATGAATTGGGGAGTAAGATGCTTTCTAAACTTCTTCCTTTTGAGCAATCAGGGGAGCCATCTCATGAAACAACTGGAGCTGGAGACCGATCCAGTGATAACGATACAGATATTGATAATCTTGACAGCGACGAGGATTTAGTAAGCAGCGATGACTATGATTCTGAAGGGAGTAATGTAAGTCATGATACTCGCAAGAAGAATAAGTGGTTCAAAAGTTTTTTGAATCCCTGGATAACCTGACCCTGGAGGAGGTGAATGATCCTGAGAGGCAGTGGCACTGCCCTGCATGCAGAGGCGGCGTTGGGGCCATCGATTGGTACAGTGGTATGCAACCCATTTTAGCCCATGCCAAAACTATCAGATCAAAGAGGGTAAAGGTGCACCGCGAACTTTCGGAAGTACTGGAAGAAGAATTGAAAAGGCGAGGAGCTGCATGCTTAAATGCCGAGGAGATGTTTGGTAAGTGGAAGGGCCTGCGAGAGACTGTAAACGATCAAGAAATTGTCTGGCCTCCTATGATTATTATTCAGAACACGCTCCTTGATCAAGATGAAAATGAACAGTGGATTGGAATGGGAAATAAAGAATTACTGGAGTATTTCAAAGGATATAAAGCCATGAAGGCAAGACATGCATATGGCCCAAAGGGACATCGTGGAATGAGTCTTCTTATATTTGAGGAATCAGCAATGGGATACTTGGAGGCAGAGCGGTTACAGAAACAGTTTTTAAAGGAAGCGAGAGGAAAAGATGACTGGGAGCGACGTCGGGTATTGTTTTATCCAGGTGGAAAACGTGTACTATATGGTTACTTCGCAACAAAAGAAGAGATGGAGATTTTCAACAGACATTCAAAAGGCAAAGCAAGGTTGAAGTATGAGATGAGATCATATCATAAAATGGTTGTGGAACCCATGAAGCAAATGGATGAGGACAATCAGAAGTTGTCTTGGTTTAAGACTAAAGTTGCTAAACAACAGGAGCACAGTAAAAATTTAGAAGAGACAGTTAGTATAGTCTCATCAAAATTGCGTATGAAAGATCAAGAAATTAAGATTATTAGACAGAGGGCTACAGAGCAGCACGAGGAATGCAAGAAAGAGATGGATTATTTGGAGCAAAGTTATAGAGAACAAATTGATCAGCTGAATGCGGATATTACAAAAAGAGAATCAGAGATGGAAGAGATGGAAGAGGAGTTCAAGAAAGCACACTTAGATCGTTGTCACCAACTTGAAGTTAACTCTGCAAAGCTCCCAAAAGGTGCACAAGTTGTGAATGACGAACAACAGGAACAACAGAGCAAAATTGCTGAAGAGATTGCAAGAAGAAAAATGATAGTTGAAGGCAGCTTCAAAGATTCTGAAGAATATGAACGTGAGAGGCAAGAACTTATTAAAATGCATGACAACAAGAGGAGGGAGATCAAGCTAAAACAGTTACAGGAAGAAGTAGCCTTAGAGAAGGAATTGGAGCGTGAGAGAGTTGAACTACTGGACAAGCATCTAAAGAGAAGGCAAGAAGTACAGAATGTTGCTGTTTAATTGTAGGGGTCGGAGAATTTCTATGCAATTAAATAAGCATACTTTATATTATAACCGTTTTTGAATTTTATGCACCCCTTGAAAAGCTACACATGAAGAGCAAGGAAACTTCTGTAGCCATCCTGTTGTTTGTATGTTGAAAAGCTACCCATGAAGAGCAAGGAAACTTCTGTAGCCATCCTGCTGTTTGTATGTTGTGATCACTAGAAATTGGAGAACTGAATCATACGGATTGTAGAATCTATTTTGCCTTTTGATGAGAATGTTGAATCATTTTCCCTGGATACAAATATCCCTTTTAAATACACACCAAAAAAAGGAAAAAAGAGGAGATTTAC

Ace file of the UniGene sp_v2.0_unigene28649 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein SUPPRESSOR OF GENE SILENCING 3 n=1 Tax=Solanum lycopersicum RepID=SGS3_SOLLC	-	-	48%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	53%
Blast2go	protein suppressor of gene silencing 3-like	-	-	69%

Source	InterPros	Term	Type	Identity
Sma3	Lipase, GDSL	IPR001087	-	-
Sma3	Zinc finger, RING-type	IPR001841	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	XS domain	IPR005380	-	-
Sma3	Zinc finger-XS domain	IPR005381	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-
Sma3	Zinc finger, RING-type, conserved site	IPR017907	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNA2

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: VVQKFFESLDNLTLEEVNDPERQWHCPACRGGVGAIDWYSGMQPILAHAKTIRSKRVKVHRELSEVLEEELKRRGAACLNAEEMFGKWKGLRETVNDQEIVWPPMIIIQNTLLDQDENEQWIGMGNKELLEYFKGYKAMKARHAYGPKGHRGMSLLIFEESAMGYLEAERLQKQFLKEARGKDDWERRRVLFYPGGKRVLYGYFATKEEMEIFNRHSKGKARLKYEMRSYHKMVVEPMKQMDEDNQKLSWFKTKVAKQQEHSKNLEETVSIVSSKLRMKDQEIKIIRQRATEQHEECKKEMDYLEQSYREQIDQLNADITKRESEMEEMEEEFKKAHLDRCHQLEVNSAKLPKGAQVVNDEQQEQQSKIAEEIARRKMIVEGSFKDSEEYERERQELIKMHDNKRREIKLKQLQEEVALEKELERERVELLDKHLKRRQEVQNVAV*

Protein Length: 448

Fln nts: AATGTATGGGGAGGCAATTACAATTATTCCTCTGAGAGGCCTGGAAATGGAGCTTGGAAGGGTAGAGCATCTTATATTGCGGATGGCCAGAAGCCTGGCCGCTGGGCGAACAACCCTCGTAGCGATAGAGACCGGGGTTTTAATCAACAGGGATGGAAGGAGTTGGGGAAACCTCATCAGAGGAATGTAAATCAAGAAAATAGGACCGCCAGAGCTAGAAATGCTAGCGGGTGGGGCCATTATGGCAGGGCAAATCAAGCAACACAGAGCAGCTGGAAGCCCGAAAACTCAGAGTCTGCCTATGGAAAGAAGGGCAAGGCAGATCTTTCTGTTCCTCCCCCACTTGCTAACGGTTGGCAATGGCGCAGCGACGCCAAGTTGGATTCTTCAATGGCATCATTACCCCGTGGAAAAGGTTCCGGACACAGGAGTGCAGATGCTAGTGAAGAGAAATATGAATTGGGGAGTAAGATGCTTTCTAAACTTCTTCCTTTTGAGCAATCAGGGGAGCCATCTCATGAAACAACTGGAGCTGGAGACCGATCCAGTGATAACGATACAGATATTGATAATCTTGACAGCGACGAGGATTTAGTAAGCAGCGATGACTATGATTCTGAAGGGAGTAATGTAAGTCATGATACTCGCAAGAAGAATAAGTGGTTCAAAAGTTTTTTGAATCCCTGGATAACCTGACCCTGGAGGAGGTGAATGATCCTGAGAGGCAGTGGCACTGCCCTGCATGCAGAGGCGGCGTTGGGGCCATCGATTGGTACAGTGGTATGCAACCCATTTTAGCCCATGCCAAAACTATCAGATCAAAGAGGGTAAAGGTGCACCGCGAACTTTCGGAAGTACTGGAAGAAGAATTGAAAAGGCGAGGAGCTGCATGCTTAAATGCCGAGGAGATGTTTGGTAAGTGGAAGGGCCTGCGAGAGACTGTAAACGATCAAGAAATTGTCTGGCCTCCTATGATTATTATTCAGAACACGCTCCTTGATCAAGATGAAAATGAACAGTGGATTGGAATGGGAAATAAAGAATTACTGGAGTATTTCAAAGGATATAAAGCCATGAAGGCAAGACATGCATATGGCCCAAAGGGACATCGTGGAATGAGTCTTCTTATATTTGAGGAATCAGCAATGGGATACTTGGAGGCAGAGCGGTTACAGAAACAGTTTTTAAAGGAAGCGAGAGGAAAAGATGACTGGGAGCGACGTCGGGTATTGTTTTATCCAGGTGGAAAACGTGTACTATATGGTTACTTCGCAACAAAAGAAGAGATGGAGATTTTCAACAGACATTCAAAAGGCAAAGCAAGGTTGAAGTATGAGATGAGATCATATCATAAAATGGTTGTGGAACCCATGAAGCAAATGGATGAGGACAATCAGAAGTTGTCTTGGTTTAAGACTAAAGTTGCTAAACAACAGGAGCACAGTAAAAATTTAGAAGAGACAGTTAGTATAGTCTCATCAAAATTGCGTATGAAAGATCAAGAAATTAAGATTATTAGACAGAGGGCTACAGAGCAGCACGAGGAATGCAAGAAAGAGATGGATTATTTGGAGCAAAGTTATAGAGAACAAATTGATCAGCTGAATGCGGATATTACAAAAAGAGAATCAGAGATGGAAGAGATGGAAGAGGAGTTCAAGAAAGCACACTTAGATCGTTGTCACCAACTTGAAGTTAACTCTGCAAAGCTCCCAAAAGGTGCACAAGTTGTGAATGACGAACAACAGGAACAACAGAGCAAAATTGCTGAAGAGATTGCAAGAAGAAAAATGATAGTTGAAGGCAGCTTCAAAGATTCTGAAGAATATGAACGTGAGAGGCAAGAACTTATTAAAATGCATGACAACAAGAGGAGGGAGATCAAGCTAAAACAGTTACAGGAAGAAGTAGCCTTAGAGAAGGAATTGGAGCGTGAGAGAGTTGAACTACTGGACAAGCATCTAAAGAGAAGGCAAGAAGTACAGAATGTTGCTGTTTAA___TTGTAGGGGTCGGAGAATTTCTATGCAATTAAATAAGCATACTTTATATTATAACCGTTTTTGAATTTTATGCACCCCTTGAAAAGCTACACATGAAGAGCAAGGAAACTTCTGTAGCCATCCTGTTGTTTGTATGTTGAAAAGCTACCCATGAAGAGCAAGGAAACTTCTGTAGCCATCCTGCTGTTTGTATGTTGTGATCACTAGAAATTGGAGAACTGAATCATACGGATTGTAGAATCTATTTTGCCTTTTGATGAGAATGTTGAATCATTTTCCCTGGATACAAATATCCCTTTTAAATACACACCAAAAAAAGGAAAAAAGAGGAGATTTAC

Fln Alignment:

AllPineV4_c5700___VVQKFFESLDNLTLEEVNDPERQWHCPACRGGVGAIDWYSGMQPILAHAKTIRSKRVKVHRELSEVLEEELKRRGAACLNAEEMFGKWKGLRETVNDQEIVWPPMIIIQNTLLDQDENEQWIGMGNKELLEYFKGYKAMKARHAYGPKGHRGMSLLIFEESAMGYLEAERLQKQFLKEARGKDDWERRRVLFYP-GGKRVLYGYFATKEEMEIFNRHSKGKARLKYEMRSYHKMVVEPMKQMDEDNQKLSWFKTKVAKQQEHSKNLEETVSIVSSKLRMKDQEIKIIRQRATEQHEECKKEMDYLEQSYREQIDQLNADITKR
B8LNA2_______________VFKKLFESMDALTFKEVNN--REWTCPACRAG--AAETHKGLKPLIDHAKNARQWNVRLHRKLVKVLEEELKRRSAAGFAVQEVYGKWLSLQEGHIDSQIVWPPIVIVENTGFKLQDNQQSSGMGNKELLEAFKAYKPTKAKQAYGPKGHRGRSLLIFEESAVGYLEADRLQKDFLKEGRGRADWDRRGTLIHPTGAKRVLYGYMANKEDMENFNKHS-GSGKSKYDMLSYKAMVIEPVKKMKENSQKLDWLDAKMLEDKAITKSLEETVDTMAARLEIQKSEVKIIIGKVAEKCDEWKKGLDHLERIYKEQIDELMGDVEKR

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
2118	11	88			0.923564
2119		88	11		0.923564
2122	11	88			0.923564
2127		11		88	0.923564
2130			12	87	0.992554
2132			85	14	0.993533
2135	14	85			0.993533
2137		85	14		0.993533
2140		85	14		0.993533
2232	80			20	0.995493
2233		80	20		0.995493
2234	20	60		20	0.992609
2235		50	50		1.0
2236	20	60		20	0.992609
2239	20		80		0.995493
2240	20	20		60	0.99255
2241	20	80			0.995493
2243			40	60	1.0
2245		40		60	1.0
2247	40			60	1.0
2248		60	40		1.0
2250		60		40	1.0
2251		40		60	1.0
2256		40	40		1.0
2307		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain