SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28591

Length: 1270 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene28591 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cyclin B1-4 n=1 Tax=Daucus carota RepID=Q2PHK7_DAUCA	-	-	40%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	80%
Blast2go	b-type cyclin	-	-	56%

Source	InterPros	Term	Type	Identity
Sma3	Armadillo	IPR000225	-	-
Sma3	U box domain	IPR003613	-	-
Sma3	Cyclin, C-terminal	IPR004367	-	-
Sma3	Basic-leucine zipper domain	IPR004827	-	-
Sma3		IPR006670	-	-
Sma3	Cyclin, N-terminal	IPR006671	-	-
Sma3	Armadillo-like helical	IPR011989	-	-
Sma3	Cyclin-like	IPR013763	-	-
Sma3	Cyclin A/B/D/E	IPR014400	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AC16

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 144 aas, your sequence is shorter than subject: 320 - 465

Fln protein: MFPNKQTQGLVQNKKMASKAAQPKAMVPPQRVSQAANNRRALGDIGNIVADVGGKCNVTKDGVNGKPLAQVSRPITRSFGAQLLAQAAANKGISAANNQTQVPVVIPKADVRGNKQRRTSKSKDIPPTTVVTNESDDCVIIEQAQRIKPTCNHNAGAAGNKEKPQLLTAKPKSLTATLTSRSAVALRGFRFDDEMTEAEEDPLPNIDGGDRDNQLAVVEYVEDIYKFYRRTEQMSCVPDYMPRQQEINAKMRAVLINWLIEVHYRFGLMPETLYLTTNLLDRYLATQLVSRSNYQLVGATAMLLRLNKKYGGNLGSRDE*

Protein Length: 321

Fln nts: TTGACTGTAATCATTTTCTTGTGCTACAATTCTCCTCGCACCTTATGTATAAGCCGATCCCGCCATCTGTAGAACAGTGAAGAAGTTTGCGAGCACGAGATACCTCGTTTCTGTGGTCGTTGTGATTTCAGGATTTTGATTTGTAGACACCGATTGGGTTTTGTGTTGGAGTTTTTTGGTTGCCAGAGCTGCCACCCCTGGGGCTCTGGATACTGCCAGATTCAACCcTGTTATCCAGGGTTTTATAACTTGTTTCTTGCTACTAGCCAGCAAATTTAGTTTGCTTAA_-_ATGTTCCCCAACAAGCAAACCCAAGGGCTTGTACAGAACAAGAAGATGGCTTCTAAGGCAGCCCAACCAAAAGCAATGGTCCCTCCCCAGAGGGTTTCTCAAGCTGCAAACAATAGGAGGGCTCTGGGAGATATTGGTAACATTGTGGCAGATGTCGGTGGTAAATGCAATGTCACCAAGGACGGAGTCAATGGGAAACCTCTTGCTCAGGTCTCTCGCCCTATTACAAGGAGTTTTGGTGCTCAGTTACTTGCACAGGCAGCTGCAAACAAGGGGATCTCTGCTGCCAATAACCAAACTCAGGTACCGGTTGTGATTCCAAAGGCAGATGTGCGGGGAAACAAGCAAAGGAGGACTAGCAAGAGCAAGGACATTCCTCCGACAACTGTAGTGACCAATGAATCAGATGATTGTGTTATTATTGAACAAGCACAGAGAATAAAACCAACTTGTAATCACAATGCTGGAGCTGCAGGGAACAAAGAGAAACCTCAATTACTAACGGCAAAGCCTAAATCACTAACAGCAACACTTACATCTAGAAGTGCAGTTGCGTTACGTGGGTTTAGATTTGATGATGAAATGACTGAGGCAGAGGAGGACCCCTTGCCAAATATTGATGGGGGGGACCGTGATAACCAGTTAGCGGTGGTTGAATATGTTGAGGATATATACAAGTTCTATCGCAGGACAGAGCAAATGAGCTGTGTGCCAGATTATATGCCCAGACAACAAGAGATTAATGCCAAAATGAGAGCAGTATTAATAAATTGGTTAATTGAGGTACATTACAGATTTGGGTTGATGCCAGAGACTTTGTATCTTACCACAAATCTTCTTGATAGATATCTTGCTACTCAACTTGTATCGAGGAGCAACTATCAATTAGTGGGTGCCACTGCAATGCTTCTAAGACTTAACAAAAAGTATGGAGGAAATTTGGGCTCCAGAGATGAATGACTTTCTTGACATCTTAGAAAAT

Fln Alignment:

AllPineV4_c5330___AAQPKAMVPPQRVSQAANNRRALGDIGNIVADVGGKCNVTKDGVNG-KPLAQVSRPITRSFGAQLLAQAAANKGISAANNQTQVPVVIPKADV-RGNKQRRTSKSKDIPPTTVVTNE-SDDCVIIEQAQRIKPTCNHNAGAAGNKEKPQLLTAKPKSLTATLTSRSAVALRGFRFDDEMTEAEEDPLPNIDGGDRDNQLAVVEYVEDIYKFYRRTEQMSCVPDYMPRQQEINAKMRAVLINWLIEVHYRFGLMPETLYLTTNLLDRYLATQLVSRSNYQLVGATAMLL
D5AC16_______________AAAKREVVPNQRVSRANNNRRALGDIGNIVVDAG--VNVTKEGVNGRKPLAQVSRPITRSFGAQLIAQAAANKGIFAANNQTQAPVVIPKAEVVRANNQRRTRKSKDIPPTTAVPEDKSDDCVIIEQPHRIKPACNRNAGAAGNKEKPRLVTAKPRSLTASLTSRTAVALHDFGFDDEMPEAEEDPLPNIDGGDLDNQLAVVEYVEGIYKFYRRTEHMSCVPDYMPRQRDINGKMRAILINWLIEVHYRFGLMPETLYLTINLLDRYLSIQRVSRNNFQLVGTTAMLL

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
424		85		14	0.993533
464		14		85	0.993533
561		11		88	0.991528
680		88		11	0.991528
832		85		14	0.993533
836		83		16	0.994512
837	83		16		0.994512
838	16			83	0.994512
840	16			83	0.994512
848			16	83	0.994512
851		16		83	0.994512
854		83	16		0.994512
856	83	16			0.994512
857			16	83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain