SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28307

Length: 3044 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28307
TGGAGTTGGGCGCGTTGACAAGGCGAGGGTTTCTTCTTTGTTCCTCTCTTATTCTCTTCATCTTGATCATTGTCCTTGCTCCACAACTGCGGCTGCGTCTTCAGATCTGTTCGTTACCTCTCGGCGGTTGCGTGTTCGAGCCTCTACGCTCTCTGCGCGTCACATTTTCTCACGGTAGAAACCAACATGGGAGAATTGAAGGAAAGCGACACTTACCAGGAAGAACTCCTTGACTATGAAGAAGAAGATGTTGCACAGGAATCAGTTGCAGCAAGGGGAGCTGGTGAAACTGTGAAAAAAGGTTATGTTGGCATTCATAGTTCAGGCTTCAGGGATTTTCTTCTGAAACCAGAGTTACTTCGTGCAATTGTAGACTCTGGATTTGAACATCCGTCAGAAGTACAACACGAGTGTATTCCCCAAGCAATCCTTGGGATGGATGTAATTTGCCAGGCAAAGTCTGGAATGGGGAAGACTGCTGTTTTTGTACTATCAACACTGCAGCAGATTGAACCTGTTGCAGGACAAGTTGCTGCTCTTGTCCTGTGTCATACTCGAGAATTGGCATATCAGATATGCCATGAATTCGAAAGGTTTATTACCTACTTGACAGACTTGAAAGTTGCTGTATTCTATGGGGGTGTAAATATCAAAACACATAAATCATTACTAAAAAATGAATGCCCCCATATAGTTGTTGGAACCCCTGGAAGGATACTGCAATTAGCAAAAGAGAAAGATCTGAATCTAAAAAATGTCCGGCATTTTATTCTGGATGAATGTGACAGGATGCTAGAATCTCTTGGTATGCTAGTAAACCACTCTTTCAAATATCTCGTGATATACCTTTTTTGTCACTTCAATATTAACTATTTTATTTTTATACATTCAAGTAGTTTTGCTGCTATATCTGACATTACTTGCTGTGTAGACATGCGGAAAGATGTCCAGGATATTTTCAAGATGACCCCTCATGACAAGCAAGTAATGATGTTCTCAGCCACACTGAGCAAGGAAATTCGGCCTGTCTGCAAAAGATTTATGCAAGATGTAAAGTCTTTTGTCCAAGTGATTTCATGTTAACTGACAGAATATTCTTTCCCTATCAAGGAAACTAGTTAGGGGGTAATGATTAACGTGTTCAATTTTCTTAGCTACATGCATATTAGTGTTATTAGTCACATGCAGTAGAGTTCAGTTTTTCTATTTGTTTAAAGTGCTGACTTGTATTTACACAGATACAATTTGACATATTATGTTCTGGATAAGGTTCTGTAGATGTCAATGTTTGTGTTTACAAATTCTTTCTTAAGTAGGCATGTTGTTAATTCCCAGTGATTTTTCTTGGAGTCTGTCAACTGTGAAGAATCTGAGATTTGTAATTAATGTAATAAATGTTCGATGTTGGGTTTGCCTGTTACTTAAGACTTCTCTTTGCATTCTCAAGAATGGAGCTGCATTAGGGGGAATGCAGGGCTGCATTTTATAATGGTGGCCATTGCACATGTGATGGAGATATGGCTACATATGTGGCCTCAGTTACTGGCTTTTGTAACAGCAATACAAATAATTAATTCATTGCAGTAGCAAAGTATTAAATTCAGAACTCAGAGACAGTATGTTGGACCACGATGCTGCTAGTTGAACATTTCAGTCCTTTTCGTTCCACATATGCTCTGCTTGGATCAAGGAGAAGCAAGTGTTGTGGCACTTTTGGCTCCTCATCGCAACACCTCACCTAATGTTGCCCTGACATTTGTTTCCATTTACAGCCTATGGAGATATACGTTGATGATGAGGCAAAGTTGACACTTCATGGACTAGTTCAGCACTACATCAAGTTGAGTGAGCTGGAGAAGAACCGTAAATTGAATGATTTGCTCGATGCGCTGGACTTCAACCAAGTGGTGATATTTGTTAAGAGCGTGAACAGAGCTGCAGAGCTAAACAAGCTACTTGTGGAGTGTAACTTTCCTTCCATATGCATTCATTCTGGGATGACACAGGAAGAAAGATTGACAAGATACAAAGGTTTCAAGGAGGGTCATAAGAGGATATTAGTTGCAACTGATTTGGTTGGTAGAGGAATCGATATTGAGCGTGTAAATATTGTTGTTAACTATGACATGCCAGATTCTGCGGATACTTACTTGCACAGAGTTGGAAGGGCTGGCAGATTTGGTACGAAGGGTCTTGCAATCACGTTTGTTTCCTCTGCTTCTGACTCAGATGTGCTCAACCAGGTTCAAGAACGTTTTGAAGTTGATATAAAGGAGCTCCCAGCACAAATAGATACATCTACATATATGCCATCATAGATGATTTGGTATGATGCTACTTTCAAAGGCTTTTCAGTTCGAGCAATTTCAAGAAGGGGTTGATCTCGTTTCTGCCTCCCCTGGAAGGATGTCTGGAAAATTTTGAACACATCTGAGCTGGAGACAGCAGCGATTTTTGTTGAGCAGCATATATTTAGATTGATCAGTTAGTTTAACCTGACCTACTCTTATGATGGGATAATCACTCTGGAAATGCTGCTGAAGACTTGACTGAATTGATATATCCTATAAATGAACACATTGCTGTTGTATTAGCATTTGTTTATTAAGTACCTGGTCTTTCTTTTCACCCTGAAATGCTTTTGTAGGCTTATTTATTCAAATCTTTTACTTGTAACATATATATTGGTGCATTATTAATGCTCTCAGAAATGCCTAAAGGCTGAGATCCAAAATAGTATGAAGTGCCCTAATTTATGGCTATATCTTGAAAATATTGTGAATTAAAGATCATATGCAGGCATACGTATCTGTTTGATCTCCGTTAATGGTAGCATTTGTTCTGCAGGCATATATCTTGATTATCGAAGTAGAATGATTGATATCGAAGTTAGATAGGATGATGTAACAGAGTAGAAAACATGTATGTTGTATAGTGGCATGTTGATTCGGCAAACCTGGCTATGTGAATATCTTGTTTAGAAGTTGAGGACATGAAAGTAATGTCAAAGACATGCATTTCTTCATATTAGAGCAATGGTAAAT

Ace file of the UniGene sp_v2.0_unigene28307 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DEAD BOX RNA helicase RH15-like protein [Arabidopsis thaliana]	-	-	75%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Blast2go	protein	-	-	82%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	82%
Blast2go	hydrolase activity	GO:0016787	Molecular Function	82%
Blast2go	nucleolus	GO:0005730	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-
Sma3		IPR014021	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9C0

Fln msg: Separated hits, possible frame ERROR between 1050 and 1761, your sequence is longer than subject: 711 - 427

Fln protein: MGELKESDTYQEELLDYEEEDVAQESVAARGAGETVKKGYVGIHSSGFRDFLLKPELLRAIVDSGFEHPSEVQHECIPQAILGMDVICQAKSGMGKTAVFVLSTLQQIEPVAGQVAALVLCHTRELAYQICHEFERFITYLTDLKVAVFYGGVNIKTHKSLLKNECPHIVVGTPGRILQLAKEKDLNLKNVRHFILDECDRMLESLGMLVNHSFKYLVIYLFCHFNINYFIFIHSSSFAAISDITCCVDMRKDVQDIFKMTPHDKQVMMFSATLSKEIRPVCKRFMQxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFHLQPMEIYVDDEAKLTLHGLVQHYIKLSELEKNRKLNDLLDALDFNQVVIFVKSVNRAAELNKLLVECNFPSICIHSGMTQEERLTRYKGFKEGHKRILVATDLVGRGIDIERVNIVVNYDMPDSADTYLHRVGRAGRFGTKGLAITFVSSASDSDVLNQVQERFEVDIKELPAQIDTSTYMPS*

Protein Length: 712

Fln nts: TGGAGTTGGGCGCGTTGACAAGGCGAGGGTTTCTTCTTTGTTCCTCTCTTATTCTCTTCATCTTGATCATTGTCCTTGCTCCACAACTGCGGCTGCGTCTTCAGATCTGTTCGTTACCTCTCGGCGGTTGCGTGTTCGAGCCTCTACGCTCTCTGCGCGTCACATTTTCTCACGGTAGAAACCAAC_-_ATGGGAGAATTGAAGGAAAGCGACACTTACCAGGAAGAACTCCTTGACTATGAAGAAGAAGATGTTGCACAGGAATCAGTTGCAGCAAGGGGAGCTGGTGAAACTGTGAAAAAAGGTTATGTTGGCATTCATAGTTCAGGCTTCAGGGATTTTCTTCTGAAACCAGAGTTACTTCGTGCAATTGTAGACTCTGGATTTGAACATCCGTCAGAAGTACAACACGAGTGTATTCCCCAAGCAATCCTTGGGATGGATGTAATTTGCCAGGCAAAGTCTGGAATGGGGAAGACTGCTGTTTTTGTACTATCAACACTGCAGCAGATTGAACCTGTTGCAGGACAAGTTGCTGCTCTTGTCCTGTGTCATACTCGAGAATTGGCATATCAGATATGCCATGAATTCGAAAGGTTTATTACCTACTTGACAGACTTGAAAGTTGCTGTATTCTATGGGGGTGTAAATATCAAAACACATAAATCATTACTAAAAAATGAATGCCCCCATATAGTTGTTGGAACCCCTGGAAGGATACTGCAATTAGCAAAAGAGAAAGATCTGAATCTAAAAAATGTCCGGCATTTTATTCTGGATGAATGTGACAGGATGCTAGAATCTCTTGGTATGCTAGTAAACCACTCTTTCAAATATCTCGTGATATACCTTTTTTGTCACTTCAATATTAACTATTTTATTTTTATACATTCAAGTAGTTTTGCTGCTATATCTGACATTACTTGCTGTGTAGACATGCGGAAAGATGTCCAGGATATTTTCAAGATGACCCCTCATGACAAGCAAGTAATGATGTTCTCAGCCACACTGAGCAAGGAAATTCGGCCTGTCTGCAAAAGATTTATGCAAGAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTTCCATTTACAGCCTATGGAGATATACGTTGATGATGAGGCAAAGTTGACACTTCATGGACTAGTTCAGCACTACATCAAGTTGAGTGAGCTGGAGAAGAACCGTAAATTGAATGATTTGCTCGATGCGCTGGACTTCAACCAAGTGGTGATATTTGTTAAGAGCGTGAACAGAGCTGCAGAGCTAAACAAGCTACTTGTGGAGTGTAACTTTCCTTCCATATGCATTCATTCTGGGATGACACAGGAAGAAAGATTGACAAGATACAAAGGTTTCAAGGAGGGTCATAAGAGGATATTAGTTGCAACTGATTTGGTTGGTAGAGGAATCGATATTGAGCGTGTAAATATTGTTGTTAACTATGACATGCCAGATTCTGCGGATACTTACTTGCACAGAGTTGGAAGGGCTGGCAGATTTGGTACGAAGGGTCTTGCAATCACGTTTGTTTCCTCTGCTTCTGACTCAGATGTGCTCAACCAGGTTCAAGAACGTTTTGAAGTTGATATAAAGGAGCTCCCAGCACAAATAGATACATCTACATATATGCCATCATAG___ATGATTTGGTATGATGCTACTTTCAAAGGCTTTTCAGTTCGAGCAATTTCAAGAAGGGGTTGATCTCGTTTCTGCCTCCCCTGGAAGGATGTCTGGAAAATTTTGAACACATCTGAGCTGGAGACAGCAGCGATTTTTGTTGAGCAGCATATATTTAGATTGATCAGTTAGTTTAACCTGACCTACTCTTATGATGGGATAATCACTCTGGAAATGCTGCTGAAGACTTGACTGAATTGATATATCCTATAAATGAACACATTGCTGTTGTATTAGCATTTGTTTATTAAGTACCTGGTCTTTCTTTTCACCCTGAAATGCTTTTGTAGGCTTATTTATTCAAATCTTTTACTTGTAACATATATATTGGTGCATTATTAATGCTCTCAGAAATGCCTAAAGGCTGAGATCCAAAATAGTATGAAGTGCCCTAATTTATGGCTATATCTTGAAAATATTGTGAATTAAAGATCATATGCAGGCATACGTATCTGTTTGATCTCCGTTAATGGTAGCATTTGTTCTGCAGGCATATATCTTGATTATCGAAGTAGAATGATTGATATCGAAGTTAGATAGGATGATGTAACAGAGTAGAAAACATGTATGTTGTATAGTGGCATGTTGATTCGGCAAACCTGGCTATGTGAATATCTTGTTTAGAAGTTGAGGACATGAAAGTAATGTCAAAGACATGCATTTCTTCATATTAGAGCAATGGTAAAT

Fln Alignment:

AllPineV4_c2099___MGELKESDTXXXXXXXXXXXDVAQESVAARGAGETVKKGYVGIHSSGFRDFLLKPELLRAIVDSGFEHPSEVQHECIPQAILGMDVICQAKSGMGKTAVFVLSTLQQIEPVAGQVAALVLCHTRELAYQICHEFERFITYLTDLKVAVFYGGVNIKTHKSLLKNECPHIVVGTPGRILQLAKEKDLNLKNVRHFILDECDRMLESLGMLVNHSFKYLVIYLFCHFNINYXXXXXXXXXXXXXXITCCVDMRKDVQDIFKMTPHDKQVMMFSATLSKEIRPVCKRFMQDxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFHLQPMEIYVDDEAKLTLHGLVQHYIKLSELEKNRKLNDLLDALDFNQVVIFVKSVNRAAELNKLLVECNFPSICIHSGMTQEERLTRYKGFKEGHKRILVATDLVGRGIDIERVNIVVNYDMPDSADTYLHRVGRAGRFGTKGLAITFVSSASDSDVLNQVQERFEVDIKELPAQIDTSTYMPS
D5A9C0_______________MGELKESDTYQEELLDYEEEDVAQESVAARGAGETVKKGYVGIHSSGFRDFLLKPELLRAIVDSGFEHPSEVQHECIPQAILGMDVICQAKSGMGKTAVFVLSTLQQIEPVAGQVAALVLCHTRELAYQICHEFERFITYLTDLKVAVFYGGVNIKTHKSLLKNECPHIVVGTPGRILALAREKDLNLKNVRHFILDECDRMLESL------------------------------------------DMRKDVQEIFKMTPHDKQVMMFSATLSKEIRPVCKRFMQDxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFMQDPMEIYVDDEAKLTLHGLVQHYIKLSELEKNRKLNDLLDALDFNQVVIFVKSVNRAAELNKLLVECNFPSICIHSGMTQEERLTRYKGFKEGHKRILVATDLVGRGIDIERVNIVVNYDMPDSADTYLHRVGRAGRFGTKGLAITFVSSASDSDVLNQVQERFEVDIKELPAQIDTSTYMPS

SNPs (tot: 52)

Position	A %	C %	G %	T %	Probability
1818		87		12	0.992554
1914	16		83		0.994512
2324		80		20	0.995493
2802	93		6		0.99903
2806	12	87			1.0
2809	90		6	3	0.996531
2811	87		6		0.993531
2812		7		92	0.999532
2816		89	10		1.0
2817	3		3	85	0.997433
2818		7		92	0.999602
2822	3			88	0.999079
2823		88	3		0.999079
2824	88		3		0.999079
2825		92	8		0.999718
2829			8	91	0.999766
2830	8			91	0.999766
2837		91	8		0.999766
2840	6	93			0.999153
2842		93	6		0.99903
2843	5			94	0.998894
2844		5	2	91	0.995912
2881		95	4		0.996252
2883			4	95	0.996252
2884	95			4	0.996252
2886	4			95	0.996252
2890		4	95		0.996599
2894			4	95	0.996599
2897		4		93	0.983471
2898			4	95	0.996599
2899	4	95			0.99692
2992	10	89			1.0
3047	75	20			1.0
3048	73	21		4	1.0
3049		21	73		1.0
3050		21	73	4	1.0
3051	90			9	0.999842
3052		71	4	23	1.0
3055	12			87	0.999964
3060	42	50			1.0
3061	64	35			1.0
3062	57	35	7		1.0
3063		50	50		1.0
3066		28	57	14	1.0
3067	83	16			0.994512
3068	66	33			1.0
3069		16	83		0.994512
3071		16		16	0.988813
3077	75	25			0.996473
3078	75	25			0.996473
3079		25	75		0.996473
3081			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain