SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28134

Length: 2072 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28134
TAAGACTATTGACAGTTCTGTGGTTGCAATCGATAGCAAGGAAATGTGCGCTTCCAACAATTTTTCTTGTAATTTTCAATCCGAACCTTCTTACCGTCATTTTAGAACTGGCAGTTTGAGTATCGTTCTTGGAATGGCATTGACTCTTAACCAATACAAACCCGAAAGATTTTTAGCAGATATAAAGGGACTAGTCAATATTTCATCATCTTGTAAACTTTGGTCCCCCAAAATTCCTATGAGTGAAGGGTATTTTGAACCTCTATGATACCCCATTTGGCACAGCAGCTCGTGCTGACATTGTGTCTGATTATTTAGAGAGCAACTGGTCACCATATAAAAACCAGGCAATCGGGGGATATTTGGCTGTCACAGGACTTGGATAAATGTTCCCTCTTGCAAGATTTTGCCCTATGGGGACAGCTCATGATACTGGAAGAACCATTTTAATGAATGGTGCTGTACCTACCAAATTGCCATACTCAGTTGAAAATGTAGTATGGCCACAAAAAGAATGCATGGATTCATCTAAAAGTGATCTGATAGTTGAGAAAGACGCCATTGATGATGATTTTAAGAATATTTTGAAAACCCTGCCACTGACTAAAGCCTTTCCAATAGTGAAGGCATTCGCAAAGAAAGAAGCCACTAGAGCCTGTGTCAGGTCCTCAGCTCAGCAGGAATGTATATCAAAAAGTATTCTATGTAATGATCGTGATTATGAATCTGCTCTTTTGCCAGGCCTTCCTGATGATGTTGCCAAGTATTGTCTTGCACTAGTACCTCGCTCAGATTTTCAGTCACTGAGATTTGTTTCGAAAGCATGGAGGAGTTTCATTCTGAGCAATGAATTTTATATGTCAAGAAAACTAGCTGGCACCCTTGAAGAATGGCTTTATGTGCTGACTGCAGATTCATGTAGTGGCAGAATGCAGTGGCAAGTTCTTGATTCTGCCCGTGGTAAATGGGAATTTCTTCCGTCCATGCCTGGTCCTTTGAAGAGAGCTTTTGGGTTTGTTGTGATAGATGAAAAACTAATGGTCATTGGTGGGTTGGTTGATAATGGCTACTCAGCAGAGGCTTCAGCAGATACATACAGTTATGATCCAATATTAAATAGGTGGAGTTTGCTTGCAAAGATGAATGTGGCACGTTTTGAATTTGCTTGTTCGGTGGTTGATGACCTTGTTTATGCAATTGGAGGTCATGGAGCAGGTGGGGAGGGTCTGTCCAGTGTTGAGGTATTTGATAGACAACGAAATAAGTGGACTCTAATAGAGAGTCTGAAACGCCCAAGATGGGGTTGCTTTGCCTGTGGGCTAGAAGATAAGCTGTATGTCATGGGCGGCCGTTCAAGTTTCACCATTGGGAATTCAAGGTATGTGGATATTTATGACCCCGAAAAGCACGTGTGGAATGAAACAAAGAATGGTTGTGTTATGGTTGTTGCTCATGCTGTTTTGAATGAAAATCTCTTTTGCATTGAGTGGAAGAACGAAAGACAATTGGCAGTCTTTGACCCAGCCAAAAATTCATGGAAAAGGGTTCCTGTTCCACTGACAAACAGCATGACTGTAGGCTTTTGTTTTGGTCTTGTCTGTGGCTCGTTATTGTTGTTTTCCACTAAAGCGGAGCCCCTGTATGAGACGCTAGTTTATGATCCAAATTCTCTGCCAGGTTCAGAGTGGCAGGTTACATCTATTAGACCCTCTGGCACTTGTATGTGTAGTGTGACTATCACAGCCTGAGATGGATTTCTGGGAATTGGCTGATACCATCTTTTCTGAGGTTTTGCTTATGTTTCGACGGGGACAACTTTTATGGTAATTCTCAATTGTGGCATGTTCAACTTTTTTAGTCAGGCATTATATTGAATATTTGCTGGAATGACATTTCTAAGGAACTCCATAACAGTTTTAGAGGCCTGGCATTATTAGATAAGCATTGTCAGTTTTATACCCCTGGCATTATTAGATGAAGCATGGTCGGCTTTATTGATAATTTATTCTGGACATAATTACGATTCAATTTGTAATCTTATCAATTTTAATCTGTGGGGCTATTATATTACG

Ace file of the UniGene sp_v2.0_unigene28134 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	F-box/kelch-repeat protein [Arabidopsis thaliana] ref\|NP_001077785.1\| F-box/kelch-repeat protein [Arabidopsis thaliana] sp\|Q9CAG8.1\|FBK28_ARATH RecName: Full=F-box/kelch-repeat protein At1g67480 gb\|AAG52295.1\|AC011020_2 unknown protein [Arabidopsis thalia	-	-	49%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	62%
Blast2go	f-box family protein	-	-	70%

Source	InterPros	Term	Type	Identity
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3	Kelch repeat type 1	IPR006652	-	-
Sma3		IPR013089	-	-
Sma3	Kelch-type beta propeller	IPR015915	-	-
Sma3	Galactose oxidase, beta-propeller	IPR015916	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NX50

Fln msg: your sequence is longer than subject: 454 - 386

Fln protein: MFPLARFCPMGTAHDTGRTILMNGAVPTKLPYSVENVVWPQKECMDSSKSDLIVEKDAIDDDFKNILKTLPLTKAFPIVKAFAKKEATRACVRSSAQQECISKSILCNDRDYESALLPGLPDDVAKYCLALVPRSDFQSLRFVSKAWRSFILSNEFYMSRKLAGTLEEWLYVLTADSCSGRMQWQVLDSARGKWEFLPSMPGPLKRAFGFVVIDEKLMVIGGLVDNGYSAEASADTYSYDPILNRWSLLAKMNVARFEFACSVVDDLVYAIGGHGAGGEGLSSVEVFDRQRNKWTLIESLKRPRWGCFACGLEDKLYVMGGRSSFTIGNSRYVDIYDPEKHVWNETKNGCVMVVAHAVLNENLFCIEWKNERQLAVFDPAKNSWKRVPVPLTNSMTVGFCFGLVCGSLLLFSTKAEPLYETLVYDPNSLPGSEWQVTSIRPSGTCMCSVTITA*

Protein Length: 455

Fln nts: TAAGACTATTGACAGTTCTGTGGTTGCAATCGATAGCAAGGAAATGTGCGCTTCCAACAATTTTTCTTGTAATTTTCAATCCGAACCTTCTTACCGTCATTTTAGAACTGGCAGTTTGAGTATCGTTCTTGGAATGGCATTGACTCTTAACCAATACAAACCCGAAAGATTTTTAGCAGATATAAAGGGACTAGTCAATATTTCATCATCTTGTAAACTTTGGTCCCCCAAAATTCCTATGAGTGAAGGGTATTTTGAACCTCTATGATACCCCATTTGGCACAGCAGCTCGTGCTGACATTGTGTCTGATTATTTAGAGAGCAACTGGTCACCATATAAAAACCAGGCAATCGGGGGATATTTGGCTGTCACAGGACTTGGATAA_-_ATGTTCCCTCTTGCAAGATTTTGCCCTATGGGGACAGCTCATGATACTGGAAGAACCATTTTAATGAATGGTGCTGTACCTACCAAATTGCCATACTCAGTTGAAAATGTAGTATGGCCACAAAAAGAATGCATGGATTCATCTAAAAGTGATCTGATAGTTGAGAAAGACGCCATTGATGATGATTTTAAGAATATTTTGAAAACCCTGCCACTGACTAAAGCCTTTCCAATAGTGAAGGCATTCGCAAAGAAAGAAGCCACTAGAGCCTGTGTCAGGTCCTCAGCTCAGCAGGAATGTATATCAAAAAGTATTCTATGTAATGATCGTGATTATGAATCTGCTCTTTTGCCAGGCCTTCCTGATGATGTTGCCAAGTATTGTCTTGCACTAGTACCTCGCTCAGATTTTCAGTCACTGAGATTTGTTTCGAAAGCATGGAGGAGTTTCATTCTGAGCAATGAATTTTATATGTCAAGAAAACTAGCTGGCACCCTTGAAGAATGGCTTTATGTGCTGACTGCAGATTCATGTAGTGGCAGAATGCAGTGGCAAGTTCTTGATTCTGCCCGTGGTAAATGGGAATTTCTTCCGTCCATGCCTGGTCCTTTGAAGAGAGCTTTTGGGTTTGTTGTGATAGATGAAAAACTAATGGTCATTGGTGGGTTGGTTGATAATGGCTACTCAGCAGAGGCTTCAGCAGATACATACAGTTATGATCCAATATTAAATAGGTGGAGTTTGCTTGCAAAGATGAATGTGGCACGTTTTGAATTTGCTTGTTCGGTGGTTGATGACCTTGTTTATGCAATTGGAGGTCATGGAGCAGGTGGGGAGGGTCTGTCCAGTGTTGAGGTATTTGATAGACAACGAAATAAGTGGACTCTAATAGAGAGTCTGAAACGCCCAAGATGGGGTTGCTTTGCCTGTGGGCTAGAAGATAAGCTGTATGTCATGGGCGGCCGTTCAAGTTTCACCATTGGGAATTCAAGGTATGTGGATATTTATGACCCCGAAAAGCACGTGTGGAATGAAACAAAGAATGGTTGTGTTATGGTTGTTGCTCATGCTGTTTTGAATGAAAATCTCTTTTGCATTGAGTGGAAGAACGAAAGACAATTGGCAGTCTTTGACCCAGCCAAAAATTCATGGAAAAGGGTTCCTGTTCCACTGACAAACAGCATGACTGTAGGCTTTTGTTTTGGTCTTGTCTGTGGCTCGTTATTGTTGTTTTCCACTAAAGCGGAGCCCCTGTATGAGACGCTAGTTTATGATCCAAATTCTCTGCCAGGTTCAGAGTGGCAGGTTACATCTATTAGACCCTCTGGCACTTGTATGTGTAGTGTGACTATCACAGCCTGA___GATGGATTTCTGGGAATTGGCTGATACCATCTTTTCTGAGGTTTTGCTTATGTTTCGACGGGGACAACTTTTATGGTAATTCTCAATTGTGGCATGTTCAACTTTTTTAGTCAGGCATTATATTGAATATTTGCTGGAATGACATTTCTAAGGAACTCCATAACAGTTTTAGAGGCCTGGCATTATTAGATAAGCATTGTCAGTTTTATACCCCTGGCATTATTAGATGAAGCATGGTCGGCTTTATTGATAATTTATTCTGGACATAATTACGATTCAATTTGTAATCTTATCAATTTTAATCTGTGGGGCTATTATATTACG

Fln Alignment:

Contig28134___IVKAFAKKEATRACVRSSAQQECISKSILCNDRDYESALLPGLPDDVAKYCLALVPRSDFQSLRFVSKAWRSFILSNEFYMSRKLAGTLEEWLYVLTADSCSGRMQWQVLDSARGKWEFLPSMPGPLKRAFGFVVIDEKLMVIGGLV-DNGYSAEASADTYSYDPILNRWSLLAKMNVARFEFACSVVDDLVYAIXXXXXXXXGLSSVEVFDRQRNKWTLIESLKRPRWGCFACGLEDKLYVMGGRSSFTIGNSRYVDIYDPEKHVWNETKNGCVMVVAHAVLNENLFCIEWKNERQLAVFDPAKNSWKRVPVPLTNSMTVGFCFGLVCGSLLLFSTKAEPLYETLVYDPNSLPGSEWQVTSIRPSGTCMCSVTITA
A9NX50_______________VLKTKPRIHATGIYISSSMRQQSLPTNAMPCNNEPESALLPGLPDDVAKHCLALVPRIHFQSLGSVCKPWRKFLQSKEFHVVRKLAGTVEEWIYVLTTDADTERTHWQVLNSVQGKWQSLPPMPGPMKTGFGYVVIDGKLLVMAGLFEDDSGTAKASANVYMYDSALNRWSELPNMKVARYGFACAEVNGLVYAVGGHGERDENLSSVEVFDPKTNEWTMVESLRRPRWGCFACGLEGRLYVMGGRSSFTIGHSRCIDVYDPEIHTWAEMKNGCVMAVAHAVLDKKLFCMEWKNERKLAVFNVVDNSWQRVPLPLTGSVTVGFCFGILNGNLLLFPTKMEPLCKTLVYDPNAMPGLEWQAIDIRPLGICICTVTITA

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
887		90	10		0.90298
1293		16		83	0.994512
1314	11		88		0.923564
1331			88	11	0.923564
1333		88		11	0.923564
1334	11	88			0.923564
1336	11		88		0.923564
1337			88	11	0.923564
1346		87		12	0.992554
1376		14		85	0.993533
1540		80		20	0.995493
1553	20	80			0.995493
1557			80	20	0.995493
1573		80		20	0.995493
1613	20	80			0.995493
1904	80		20		0.995493
1905	80	20			0.995493
1906			20	80	0.995493
1907	20	80			0.995493
1908	20			80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain