SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28038

Length: 1406 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene28038 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	heat shock protein 21 [Arabidopsis thaliana] sp\|P31170.1\|HS25P_ARATH RecName: Full=25.3 kDa heat shock protein, chloroplastic; Short=AtHsp25.3; Flags: Precursor emb\|CAA38036.1\| heat shock protein [Arabidopsis thaliana] gb\|AAA32818.1\| heat shock protein 21	-	-	2.0e-31	40%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	69%
Blast2go	heat shock protein 21	-	-	0.0	59%

Source	GOs	Term	Type	e value	Identity
Blast2go	response to stress	GO:0006950	Biological Process	0.0	59%
Blast2go	response to abiotic stimulus	GO:0009628	Biological Process	0.0	59%

Source	InterPros	Term	Type	e value	Identity
Sma3	Heat shock protein Hsp20	IPR002068	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NNU0

Fln msg:

Fln protein: MACPLSYLPATTSAYSTPISPRFRASVPWKLGFTRTSINVATFFPVGKRLQSNVSWSSPSNQRLPNGVVVRAGSNREGVDSAIDGHEKLKKEISPFGVVDPLLPKRTMRQMVDVMEVQEVSVRTPWDIVENEDELKMRFDMPGLSRDDVKVSVVEDRVLVIEDREEREKDSWPSHSRYHTRLVLPENYETSKIKTELKIGVLNITIPKVKVVSKMKDHSQNREAEKMQAEYKNGVLNIIIPQSKVESKVTDLSVN*

Protein Length: 257

Fln nts: ATGGGGTCAACTTCACAGGTAAGCCAACTGTGTATTACCTTATGCCACTTTCAAGCTTACGTGAATTTGGGACTCATCTTTCATTTGGACTACTTGTACCTACGGCACGGCCCCGATTTCATCTGTTAAGGTTTTCCTCCGACTTGTCAACTTCACAGGTCCGGCTCGGCCCACCTCGACTCGCTAAAGA_-_ATGGCTTGCCCGCTGTCTTACTTGCCTGCAACTACTTCTGCTTATTCGACGCCAATATCCCCTAGATTTCGAGCCTCTGTTCCCTGGAAGCTTGGATTTACTCGTACTAGTATTAATGTTGCTACTTTCTTTCCAGTGGGCAAACGGTTGCAGTCCAATGTAAGCTGGAGTTCTCCTTCCAATCAACGACTTCCAAATGGTGTCGTTGTCAGAGCCGGAAGCAACAGGGAAGGCGTTGACAGTGCCATAGACGGTCACGAGAAGCTCAAGAAGGAGATTTCACCGTTTGGTGTCGTGGACCCCTTGTTGCCAAAGAGGACGATGCGCCAGATGGTTGATGTGATGGAGGTCCAGGAAGTGTCGGTGCGAACCCCATGGGACATTGTGGAAAACGAAGATGAACTGAAAATGAGATTTGACATGCCCGGATTGTCCAGGGACGATGTGAAGGTGTCTGTTGTTGAGGATCGCGTTCTGGTGATCGAAGATAGAGAAGAGAGGGAAAAGGACTCTTGGCCTTCGCACAGCAGATACCATACTCGCCTGGTGTTGCCTGAAAACTATGAGACGAGCAAAATCAAGACGGAATTGAAGATTGGTGTGCTGAATATCACGATCCCCAAGGTGAAGGTGGTGTCGAAGATGAAGGACCATTCTCAGAACCGTGAGGCGGAGAAAATGCAGGCAGAATACAAGAATGGAGTGCTGAATATCATCATCCCCCAGAGTAAAGTGGAGTCCAAGGTTACGGATCTCTCCGTTAACTAA___CGGAAGTTGAAGGCCACAAAGAGAGCTCTTCGCACACAATAAAATGAGCGTTCTCTCCAATTTGACCCGTCTTTCTAATATGCTATTTTCTCATAGTGTGGGTGTGGGTGTGAGTATATAATAGGTATTCGTTGGCCTTAGTAAGTATATAATTTCGCATTCGTCCAACATGTAATAAGTTTATAATTTCGCATTCGTGTGATATGTATTCCGGATAAAGGATGCCTCTTTTGCGGTATCCCAAAAATAAGAACAGCCTTTTGGATATCTGGTAAGAGCTCTGCGTAATGATTTGCCATCTGTACACGTGTTTAATATACAACAAAGTATATTTAGTGTAATTGTTTAATATACATCAAGATACTTTTTGGGTGGAATTCTAACGGATTATGTTTTTAACTCTTAATTTTATCAATAATATTATCACGGATCAAAAGATCAAATTTAC

Fln Alignment:

Contig28038___MACPLSYLPATTSAYSTPISPRFRASVPWKLGFTRTSINVATFFPVGKRLQSNVSWSSPSNQRLPNGVVVRAGSNREGVDSAID---------GHEKLKKEISPFGVVDPLLPKRTMRQMVDVMEVQEVSVRTPWDIVENEDELKMRFDMPGLSRDDVKVSVVEDRVLVIEDREEREKDSWPSHSRYHTRLVLPENYETSKIKTELKIGVLNITIPKVKVVSKMKDHSQNREAEKMQAEYKNGVLNIIIPQSKVESKVTDLSVN
A9NNU0_______________MACSLSYLTAVASTCTTPISARNGASGPWKLGCTRSSINVVTFFPVGKRLQSNVGWSSSSHQRLTNALVVRAESGKENVAGAIDLKVTKSKNVDDEKHRAEVSPFGLVDALLPKRTMRQMLDTMEGQASSVRTPWDIIENENELKMRFDMPGLSKDDVKVSVVEDRVLVIEEREERQKDLWSFYSSYHTRLVLPENYETNEIGAELNNGVLKITIPKTKVMSKINDLPGNREMDKILAEFKNGVLNIIIPISKVVSKVKDLSVN

SNPs (tot: 89)

Position	A %	C %	G %	T %	Probability
258		83		16	0.994512
264		83		16	0.994512
282	16			83	0.994512
329		11		88	0.923564
399		88		11	0.923564
440		88	11		0.923564
683		11	88		0.923564
684		11		88	0.923564
686		11		88	0.923564
687	12	87			0.992554
689			87	12	0.992554
705		33	66		0.997452
738	75			25	0.996473
764		75	25		0.996473
958		83	16		0.994512
990		83		16	0.994512
1011	83		16		0.994512
1012		83		16	0.994512
1014	83	16			0.994512
1018		83		16	0.994512
1020			16	83	0.994512
1021	16			83	0.994512
1022		16		83	0.994512
1026	83			16	0.994512
1027		16	83		0.994512
1028	16			83	0.994512
1032	20			80	0.995493
1033	20			80	0.995493
1035	80	20			0.995493
1037	20			80	0.995493
1038	20		80		0.995493
1068		80		20	0.995493
1126	85		14		0.993533
1254	16		83		0.999991
1255	83		16		0.999991
1256	83			16	0.999991
1257	16		83		0.999991
1258	16			83	0.999991
1261	83	16			0.999991
1262	16	83			0.999991
1263	83		16		0.999991
1265	16		83		0.999991
1267		83		16	0.999991
1269	16		83		0.999991
1271	18		81		0.999994
1272	18		81		0.999994
1274	81			18	0.999994
1277	18	81			0.999994
1278		27	72		1.0
1280	18		9	72	0.999994
1281	72		9	18	0.999994
1287	18			81	0.999994
1288			81	18	0.999994
1289	81	18			0.999994
1291	81		18		0.999994
1292		18		81	0.999994
1293	18	81			0.999994
1294	18		81		0.999994
1295	18		81		0.999994
1296			81	18	0.999994
1298		81		18	0.999994
1299		81		18	0.999994
1300	18		81		0.999994
1301		18		81	0.999994
1302			81	18	0.999994
1303		81		18	0.999994
1305		18	81		0.999994
1306		72		27	1.0
1309	9		72	18	0.999994
1310		18		81	0.999994
1311	81		18		0.999994
1312		81		18	0.999994
1314	90	10			0.90298
1315		10	90		0.90298
1316		10		90	0.90298
1317	90			10	0.90298
1318			90	10	0.90298
1319			10	90	0.90298
1320		90	10		0.90298
1323	90	10			0.90298
1326	10		90		0.90298
1328	88			11	0.923564
1329	87			12	0.992554
1330			87	12	0.992554
1331	87	12			0.992554
1334	85		14		0.993533
1335			85	14	0.993533
1337		71	14		0.984337
1339	14	85			0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain