SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27850

Length: 1430 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene27850 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	branched-chain alpha-keto acid decarboxylase E1 beta subunit [Arabidopsis thaliana] gb\|AAD10651.1\| branched-chain alpha-keto acid decarboxylase E1 beta subunit [Arabidopsis thaliana] gb\|ABD59079.1\| At1g55510 [Arabidopsis thaliana] gb\|AEE33256.1\| branched-	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	38%
Blast2go	branched-chain alpha-keto acid decarboxylase e1 beta subunit	-	-	94%

Source	GOs	Term	Type	e value	Identity
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	0.0	94%

Source	InterPros	Term	Type	Identity
Sma3	Transketolase, C-terminal	IPR005476	-	-
Sma3	Transketolase-like, C-terminal	IPR015941	-	-
Sma3	Transketolase-like, pyrimidine-binding domain	IPR005475	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPU2

Fln msg: your sequence is shorter than subject: 359 - 378

Fln protein: MATIARKLGWRRAMSTLSSSSSIQSVNSSIVQSEGKRINLFTAINQALHIALETDPRSYVFGEDVSFGGVFRCTTGLTERFGKHRVFNTPLCEQGIVGFGIGLAAMGNQAIVEIQFADYIYPAFDQIVNEAAKYRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHAPGIKVVIPRSPMQAKGLLLASIRDPNPVLFFEPKWLYRLAVEEVPDHDYTLPLSQAEVIREGTDITLVGWGAQLAIMEQACLDASKEGISCELIDLRTLIPWDKETVEASVRKTGRLLVSHEAPVTGGFGAEIAASVVERCFLRLEAPVARVCGLDTPFPLVFEPFYMPTKNKVLDAIRATVNY*

Protein Length: 360

Fln nts: ATGGGGGCCCAACATTAAACAACCGTCGTGACtTTGcaTGCgtGGCATAGGTAGAATTCTATTCTCACGCTTGAGGAGAGGTTAGCCCAGATCGTCGAGGAGAAGCGACAGAGGCGACAGGA_-_ATGGCGACGATTGCGAGGAAATTGGGATGGAGGAGGGCGATGAGCACATTGTCTTCATCTTCTTCAATCCAATCTGTGAATTCGTCGATAGTTCAATCAGAGGGGAAGAGAATTAATCTTTTCACGGCCATAAATCAGGCCCTTCACATTGCTCTTGAAACCGATCCTCGGTCTTATGTGTTTGGGGAGGATGTGAGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTAACAGAGCGATTTGGTAAACATCGAGTTTTTAACACTCCCCTTTGTGAGCAGGGCATTGTTGGATTTGGAATTGGATTGGCTGCAATGGGAAATCAGGCAATAGTGGAAATTCAATTTGCAGATTACATTTACCCTGCATTTGACCAGATTGTTAATGAAGCTGCAAAATATCGATATCGAAGTGGGAATCAATTCAATTGTGGAGGACTTACTATAAGAGCTCCTTACGGTGCTGTTGGCCATGGTGGGCACTATCACTCACAATCACCAGAGGCCTTCTTTTGTCATGCGCCAGGTATCAAGGTTGTTATACCACGGAGCCCAATGCAAGCAAAAGGTTTACTTCTTGCATCAATACGCGATCCAAATCCAGTGCTCTTTTTTGAGCCAAAGTGGCTCTATCGTCTGGCAGTCGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGACATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATGGAACAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCTTGTGAGCTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTCAGAAAGACTGGGAGACTTCTTGTTAGTCATGAGGCTCCTGTTACTGGGGGCTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAGGTTAGAAGCTCCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCCATCAGAGCAACTGTAAATTACTAG___CATTGCAGAATCCTTGCATCTGATTTCCCCGTTGTAAAACAAAGCGCTTCTATAGCTGATCTGCGTTCAGATATTTTGCAGAGGGTGCTGTACTTTATACCTGTAACTGACACAAATTAAATGATTAAATGATTTACTTGACAAATATTGAAAATTCAAAGTTCAATTATTGTACATAAAAAAACTGTTGAATATATTGAAAAATATCTTCAAGATCATTGGGGAAGTTTG

Fln Alignment:

Contig27850___KRINLFTAINQALHIALETDPRSYVFGEDVS-FGGVFRCTTGLTERFGKHRVFNTPLCEQGIVGFGIGLAAMGNQAIVEIQFADYIYPAFDQIVNEAAKYRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHAPGIKVVIPRSPMQAKGLLLASIRDPNPVLFFEPKWLYRLAVE---EVPDHDYTLPLSQAEVIREGTDITLVGWGAQLAIMEQACLDASKEGISCELIDLRTLIPWDKETVEASVRKTGRLLVSHEAPVTGGFGAEIAASVVERCFLRLEAPVARVCGLDTPFPLV--FEPFYMPTKNKVLDAIRAT
B8LPU2_______________KEMTVRDALNSAIDEEMSADPKVFLMGEEVGEYQGAYKISKGLLQKFGPDRVLDTPITEAGFTGIGVGAAFYGLRPIVEFMTFNFAMQAIDQIINSAAKTYYMSAGQISVP-IVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLTPYSAEDSRGLMKAAIRDPDPVIFLENELLYGESFPVSAECLDPSFCLPIGKAKIEREGKDVTITAFSKMVGYALQAAQELEKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGIGAEICTSVVEESFEYLDAPVERITGADIPMPYAANLERLAVP---QVEDIIRAS

SNPs (tot: 38)

Position	A %	C %	G %	T %	Probability
757		10	90		0.90298
759	10	90			0.90298
760	10			90	0.90298
761		90	10		0.90298
926	81	18			0.999994
1266		90	9		0.999872
1268		90	9		0.999872
1270	4	9		85	0.999726
1271	9	85	4		0.999726
1275	9	90			0.999872
1276	9		4	85	0.999726
1277	4	85	9		0.999726
1278		90	9		0.999872
1282	91		8		0.999766
1283	8			91	0.999766
1285		91	8		0.999766
1292	23	76			1.0
1366		14	85		0.999981
1367	85	14			0.999981
1368		14	85		0.999981
1369	85	14			0.999981
1388	37	62			1.0
1389		62	37		1.0
1390	57			42	1.0
1391		57	42		1.0
1392	42		57		1.0
1394	42			14	1.0
1395	14		42	42	1.0
1396	42	57			1.0
1398	85	14			0.993533
1399	85	14			0.993533
1400		14	42	28	1.0
1401	14		71		0.984337
1404	60	20		20	0.99255
1405		80	20		0.995493
1406		20	80		0.995493
1407	80		20		0.995493
1408		80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain