SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27742

Length: 1762 nt

Origin of reads: Biogeco1 v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene27742

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Putative receptor protein kinase [Oryza sativa Japonica Group] gb\|AAP53098.1\| Leucine Rich Repeat family protein [Oryza sativa Japonica Group]	-	-	43%
FL-Next	tr=Somatic embryogenesis receptor-like kinase 1; Pinus massoniana.	-	-	35%
Blast2go	probable lrr receptor-like serine threonine-protein kinase at3g47570-like	-	-	61%

Source	InterPros	Term	Type	Identity
Sma3	C2 calcium-dependent membrane targeting	IPR000008	-	-
Sma3	EGF-type aspartate/asparagine hydroxylation site	IPR000152	-	-
Sma3	ATPase, V0 complex, proteolipid subunit C	IPR000245	-	-
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	ATPase, F0 complex, subunit A	IPR000568	-	-
Sma3	Intradiol ring-cleavage dioxygenase, C-terminal	IPR000627	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	Glutathione peroxidase	IPR000889	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Leucine-rich repeat, typical subtype	IPR003591	-	-
Sma3	Putative S-adenosyl-L-methionine-dependent methyltransferase	IPR004159	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Copine	IPR010734	-	-
Sma3	ATPase, V0 complex, proteolipid subunit C, eukaryotic	IPR011555	-	-
Sma3	Domain of unknown function DUF1618	IPR011676	-	-
Sma3		IPR012335	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	ABC transporter, conserved site	IPR017871	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3	C2 membrane targeting protein	IPR018029	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-
Sma3	EGF-like calcium-binding, conserved site	IPR018097	-	-
Sma3	Glycoside hydrolase, family 1, active site	IPR018120	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5FY57

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 197 aas, putative chimeric sequence, your sequence is shorter than subject: 348 - 626

Fln protein: PEIAQLHDLVFYLDLSWNLLEGSLPMGIGKITMVQAIDISANKLTGVIPPTLGSCSELQSLNLSQNAFQGFIPDSLGNLQSLVKLDLSSNSLLGTIPESLEKLKMLQYLNFSFNNLTGEIPKGGIFANQTISIYFIGNPGLCGPQVFRLTACPSTKGHFPFVKKVLLPVSGAIVFILCCLLLEFFRRRNIHMQNMDLPRALIQRISYQELHISTNGFSEANLIGAGSFGSVYKGILSDGTLVAVKVLDLQNDQVEKSFKAECSVLKKVRHRNLVRIITSCSNLHFKGLVFQFMCNGSLEKHLYPNRDDNNNEDVCQLGLKTRLNIAIDVAHAMEYLHHYSFVASSAL*

Protein Length: 349

Fln nts: CCAGAAATTGCTCAACTTCATGATTTAGTTTTCTACTTGGATTTGTCATGGAATTTATTAGAAGGCTCTTTGCCCATGGGGATTGGTAAAATTACAATGGTTCAAGCTATAGACATTTCTGCCAATAAGTTGACTGGGGTCATTCCACCTACACTAGGAAGCTGCAGCGAATTGCAATCATTGAATTTATCTCAAAATGCCTTCCAAGGATTTATTCCAGATTCACTTGGAAATTTGCAAAGTCTTGTGAAGCTAGATCTCTCTTCCAACTCCTTATTGGGTACAATACCAGAATCACTTGAGAAGTTGAAAATGCTTCAATATTTGAATTTCTCTTTCAACAATCTAACAGGGGAAATTCCAAAAGGTGGTATATTTGCAAATCAAACAATTTCCATTTATTTCATTGGAAATCCTGGCCTTTGTGGGCCACAAGTCTTCAGGTTAACTGCATGTCCATCAACCAAAGGTCATTTTCCCTTTGTGAAAAAGGTATTATTACCAGTGAGTGGAGCTATTGTTTTTATACTATGCTGTTTACTTTTGGAATTTTTTCGGAGAAGGAATATCCATATGCAAAACATGGATTTGCCACGAGCTCTAATTCAAAGGATCTCATATCAAGAACTTCACATATCAACTAATGGATTTTCTGAGGCCAACTTGATTGGAGCCGGTAGTTTTGGTTCAGTGTACAAGGGAATTTTGAGTGATGGCACACTTGTTGCTGTCAAAGTTCTAGACTTGCAAAATGACCAGGTTGAGAAGAGCTTTAAAGCAGAATGTAGTGTCTTGAAGAAGGTCCGACATCGGAATTTGGTGAGGATCATAACCTCATGTTCCAACCTCCATTTCAAAGGTTTAGTGTTCCAATTTATGTGTAATGGAAGCTTAGAGAAGCATCTTTATCCTAACAGAGATGACAACAACAATGAGGATGTGTGTCAGCTAGGGTTGAAAACAAGATTAAACATTGCCATAGATGTGGCCCATGCCATGGAATATCTTCATCATTATTCTTTTGTTGCAAGTAGTGCATTGTGA___TATAAAACCAAATAATGTGCTTTTAGATGAAGACATGTCAGGCCATGTAACAGATTTTGGTATTGCGAGATTAATTGGTGCATCCTCTACTGATTCACTTGCCTCAACATTTTCTTTGAAAGGATCAGTGGGCTACATTGCCCCAGAGTATGGATTAGGTGAGAGAGTTTCAACCAAAGGAGATGTTTACAGCTATGGCATTTTGTTATTGGAAATGCTTACTAGAAAGAGACCAACAAGTGACATATTTTTTGGAGACCTAACATTGCACAAGTGGGTTAACTTAGCATTTCCAAACAGAGTGAAGGAGGTAATTGATAACAGTCTTTTAACCGGGATGGATGGAGATGAATTTGAAGAAGATAATGTATATAAATGCATACTTTCTTTGATAGAAGTTGGTTTGTTTTGTTCAAAAGATTCCCCTGAAGAAAGACCAACTATGAAAGATGTGGTAATAATATTGGTGAATATTAAAAAGCATTTGATGACAAATACAGTGGCTTCTAGGAGATTGACAAGGAGCATATCTGATTTGCTTAACAACACAAATGCAACCAGGAATGATGCCCCTGCATCAAATGACCAAAGTTCTACATTTTAGTTTTTGAAATCAAAATAGCAAGAGATTTCAGTCTTGCCATTATTCTGGCAACAGGCAGAACATTTTGTTAATATTTATTATTAGTATGTGATTATTGTCATTAAGAAAATTAGA

Fln Alignment:

Contig27742___VFYLDLSWNLLEGSLPMGIGKITMVQAIDISANKLTGVIPPTLGSCSELQSLNLSQNAFQGFIPDSLGNLQSLVKLDLSSNSLLGTIPESLEKLKMLQYLNFSFNNLTGEIPKGGIFANQTISIYFIGNPGLCGPQVFRLTACPSTKGHF---PFVKKVLLPVSG-----------------------AIVFIXXXXXXEFFRRRNIHMQNMDLPR--------ALIQRISYQELHISTNGFSEANLIGAGSFGSVYKGILSDGTLVAVKVL-DLQNDQVEKSFKAECSVLKKVRHRNLVRIITSCSNLHFKGLVFQFMCNGSLEKHLYPNRDDNNNEDVCQLGLKTRLNIAIDVAHAMEYLHHY
D5FY57_______________VIRVDLGNAQLSGSLVPQLGQLNNLQYLELYSNNISGPIPSDLGNLTNLVSLDLYLNNFTGLIPESLGKLSRLRFLRLNNNSLVGRIPMSLTTITALQVLDLSNNNLTGEVPANGSFSLFT-PISFGGNQYLCGPVAQK--PCPGSPPFSPPPPFVPPP--PVAGSNGARVQSSSSTGAIAGGVAAGAALLFAAPAIGFAWWRRRKPQEHFFDVPAEEDPEVHLGQLKRFSLRELQVATDGFSNRNILGRGGFGKVYKGRLADGSLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCL---RERAQNDP--PLDWPTRKRIALGSARGLSYLHDH

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
585		25		75	0.996473
599	20		80		0.995493
650	20			80	0.995493
678	16		83		0.994512
843		75		25	0.996473
1268	88	11			0.923564
1337	10	90			0.90298
1370		88	11		0.923564
1395	88		11		0.923564
1437	14			85	0.993533
1438	85			14	0.993533
1668	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain