SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27731

Length: 2648 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene27731
AAGAACCATAATGTTACTTAAACTGCAGAATTTTGTTTCTTTCCAATTGTTCCATGTTCTAAGACCTTGCAACCACCGTGGACAATCTACTCGCTTCGAGGTCAGTAATCTTTTTGGGAACATAAGGCAATAGGCGACCGGCCATGCATGTTGAGCCACTGTCTCTCCTCATGTGCTCCGAGACAGGATGCTTGGAGCCTCAAGATGTCAAAAAGAGTAAACATGCCACTGTGTACAGTCCCTACTTTTCGGGCTGTTGTCACCTGCAAATCTCTATTCAGTTCAGGACAATTAAATCATGCACTTTTACTGTCCATTAAACCTGCAGCTTTATCATTGACCAGGGCATACAATGCCTTGCAAGGGAGCCCTCTTGTGCTTCAGTGTGCCCCAGCAGTTGGGCTTATGGCATTTTCTGTGTGGGGACTTGAGCCATCTCTGCAAGTTATTCGTCATTTGATACTTCGTCGGAATGCAAACAATTGGAAAAGAAGCAGCACATATCACATCATGACCTCCTATTTACAGCCAATTCTGCTATGGTTAGGTGCAATATTGATCTGCAGGGCTTTTGATCCTGTTGTGTTATCCTCTGAAACAAGCCAAGCTGTCAAGCAGCGGCTTGTAAACTTTGTGAGATCGCTATCAACTGTCTTGGCTTTTGCATATTGTATATCTAGCTTGATTCAGCAGGCACAGAAATTTGTAATGGAGCACAATGACACCCAGGACACAAGACATGTTGGATTTAACTTCATTGGAAAAGCTATTTATACTGCAGTGTGGGTGGCAGCGGTATCCTTGTTCATGGAGCTATTGGGGTTTTCAACACAGAAATGGATCACTGCTGGAGGGCTGGGAACAGTTTTGTTGACTCTTGCTGGTCGTGAGATATTTACCAATTTCCTGTCAAGTGTAATGATCCATGCAACTCGCCCTTTTGTTGTGAGTGAGTGGATTCAAACCAAGATCGACGGCTATGAAGTTTCTGGAACTGTGGAACATGTTGGTTGGTGGTCTCCGACTATTGTCAGAGGAGATGATCGGGAGGCTGTGCATATTCCAAATCACAAGTTCACTGTGAGTGTTGTTCGGAATCTTACTCAAAAGAGCCATTGGCGCATTAAGACTCATCTCGCTATCAGCCATCTAGATGTCAGCAAAATTGGTAATATTGTAGCTGATATGCGGAAAGTCTTAGCAAAAAATCATCAAGTGGAGCAACAACGGTTGCATCGAAGAGTATTTCTGGATAATATCGATCCAGAAAATCAGGCACTTTTGATTTTGGTTTCTTGCTTTGTAAAGACCCCCCACTTTGAAGAGTATCTTTGTGTTAAGGAAGCCATTTTATTGGACCTTCTAAGAGTTATCAGTCATCATCGTGCCCGATTGGCTACTCCAATCCGTACTGTACAGAAAGTATATGGTGACAGCGAGACACAGAATATACCATTTGAGGAAACCATAATCAGAAGAGGAGTGCCTAAGATGATTGAAAGTCCAAAATTAACTGTCGAGGATAAAGTAAAACAACGTTCTCTTCGTAGCAAGGAAGAGTTGAATACTGTTCATCCAAAGGCAGTGTCTGTAGAGGCTAAAGGGACTGAACCTGGAGTTTCTTCCAATGAGAATACAAAACCTAGTAGCTTGGATGGAAAGAATATTCACAAGCAGAAAGAGAAAGAGAAAGATAGGGACCATGCTCCTTCAGACTTGCAACCAAAGATGCTAGATGCAGTTCAAACATCACATACAAAGCCATCTCTTGAAGGTTTAGATTCAATGGGACTGAATTCCAAGGACATCACATTGCTTAGGGCAGCATTTGAGAAACCTCCAGTTATTATTCCTGAAGCACCCAGGGATCCTGTAATTCCAAAGAATGAGAATGGAAATAATGAGAAGAACAATGCAAGGAGTGAGAAGAATGCTACAACTAATGAGAAGTCAAACTCACAAGTTTTTGAAAAGCAATCATATCAAAATATTAAGGGAGGTGAAAAGAATGGGAGCAAGCTATCTATTGAAGTTGATAGTTCAACCATAGAAATAAATAAAGGTAAGGCCAACTCTGTAGGTGAATTGGAAATCGAACTAAGACCTGAGAAAAACAAACAAAAAGGCCAGGGGTCTTCCTCTGTACCATCTTCCAAGCATGAGAATGAACGATCTGTTAATTCTGCCTCAATCTCAAGACCTCTGCTGAAACAAAATTTACCTCTGGGTGTTGCTCTGGACGGGCCAAAAAGAACTCTTCCAATTGAGGGAGAAATTACTTCACAAGTGGAGCAGAAAGAACTTGCACCATTGCGAAATGGTAATAGTAGTTCTGGAGTCAAGGATAGGAGGGAACCTCAGTCACCATCCTCAAGCTCTGTTCATTCTGATGAAAAAGATCAGTAGAGGTGATGACAGTAGTATCTGGTAACTTATAGAACCCTGGCATGTTTGTTAATTTACTAACTAATCCATCTTGTTAGGATGCCATAGAATGGGCAGAATAAAAGGGTAGTATCAGAGTTTTCTGAATGTCTTTTGAATCTGATCACTATATAGTATTCTTATATATTATATAGATTTGTAATATAAAGTATAATGATATTACTTGGGTCCAAAAATCAACGAATTAACTTACCCAAGCCTTT

Ace file of the UniGene sp_v2.0_unigene27731 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	MSCS-like 2 protein [Arabidopsis thaliana] dbj\|BAH20174.1\| AT5G10490 [Arabidopsis thaliana] gb\|AED91552.1\| MSCS-like 2 protein [Arabidopsis thaliana]	-	-	53%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	53%
Blast2go	mscs-like 2 protein	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	plastid organization	GO:0009657	Biological Process	70%
Blast2go	ion channel activity	GO:0005216	Molecular Function	70%
Blast2go	plastid envelope	GO:0009526	Cellular Component	70%
Blast2go	chloroplast	GO:0009507	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Transposase, MuDR, plant	IPR004332	-	-
Sma3	Zinc finger, PMZ-type	IPR006564	-	-
Sma3	Mechanosensitive ion channel MscS	IPR006685	-	-
Sma3	Zinc finger, SWIM-type	IPR007527	-	-
Sma3	Extracellular solute-binding protein, family 3, conserved site	IPR018313	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: tr_plants

Fln subject: F6H132

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is longer than subject: 753 - 709

Fln protein: MLSHCLSSCAPRQDAWSLKMSKRVNMPLCTVPTFRAVVTCKSLFSSGQLNHALLLSIKPAALSLTRAYNALQGSPLVLQCAPAVGLMAFSVWGLEPSLQVIRHLILRRNANNWKRSSTYHIMTSYLQPILLWLGAILICRAFDPVVLSSETSQAVKQRLVNFVRSLSTVLAFAYCISSLIQQAQKFVMEHNDTQDTRHVGFNFIGKAIYTAVWVAAVSLFMELLGFSTQKWITAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVSEWIQTKIDGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVSVVRNLTQKSHWRIKTHLAISHLDVSKIGNIVADMRKVLAKNHQVEQQRLHRRVFLDNIDPENQALLILVSCFVKTPHFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKVYGDSETQNIPFEETIIRRGVPKMIESPKLTVEDKVKQRSLRSKEELNTVHPKAVSVEAKGTEPGVSSNENTKPSSLDGKNIHKQKEKEKDRDHAPSDLQPKMLDAVQTSHTKPSLEGLDSMGLNSKDITLLRAAFEKPPVIIPEAPRDPVIPKNENGNNEKNNARSEKNATTNEKSNSQVFEKQSYQNIKGGEKNGSKLSIEVDSSTIEINKGKANSVGELEIELRPEKNKQKGQGSSSVPSSKHENERSVNSASISRPLLKQNLPLGVALDGPKRTLPIEGEITSQVEQKELAPLRNGNSSSGVKDRREPQSPSSSSVHSDEKDQ*

Protein Length: 754

Fln nts: AAGAACCATAATGTTACTTAAACTGCAGAATTTTGTTTCTTTCCAATTGTTCCATGTTCTAAGACCTTGCAACCACCGTGGACAATCTACTCGCTTCGAGGTCAGTAATCTTTTTGGGAACATAAGGCAATAGGCGACCGGCCATGC_-_ATGTTGAGCCACTGTCTCTCCTCATGTGCTCCGAGACAGGATGCTTGGAGCCTCAAGATGTCAAAAAGAGTAAACATGCCACTGTGTACAGTCCCTACTTTTCGGGCTGTTGTCACCTGCAAATCTCTATTCAGTTCAGGACAATTAAATCATGCACTTTTACTGTCCATTAAACCTGCAGCTTTATCATTGACCAGGGCATACAATGCCTTGCAAGGGAGCCCTCTTGTGCTTCAGTGTGCCCCAGCAGTTGGGCTTATGGCATTTTCTGTGTGGGGACTTGAGCCATCTCTGCAAGTTATTCGTCATTTGATACTTCGTCGGAATGCAAACAATTGGAAAAGAAGCAGCACATATCACATCATGACCTCCTATTTACAGCCAATTCTGCTATGGTTAGGTGCAATATTGATCTGCAGGGCTTTTGATCCTGTTGTGTTATCCTCTGAAACAAGCCAAGCTGTCAAGCAGCGGCTTGTAAACTTTGTGAGATCGCTATCAACTGTCTTGGCTTTTGCATATTGTATATCTAGCTTGATTCAGCAGGCACAGAAATTTGTAATGGAGCACAATGACACCCAGGACACAAGACATGTTGGATTTAACTTCATTGGAAAAGCTATTTATACTGCAGTGTGGGTGGCAGCGGTATCCTTGTTCATGGAGCTATTGGGGTTTTCAACACAGAAATGGATCACTGCTGGAGGGCTGGGAACAGTTTTGTTGACTCTTGCTGGTCGTGAGATATTTACCAATTTCCTGTCAAGTGTAATGATCCATGCAACTCGCCCTTTTGTTGTGAGTGAGTGGATTCAAACCAAGATCGACGGCTATGAAGTTTCTGGAACTGTGGAACATGTTGGTTGGTGGTCTCCGACTATTGTCAGAGGAGATGATCGGGAGGCTGTGCATATTCCAAATCACAAGTTCACTGTGAGTGTTGTTCGGAATCTTACTCAAAAGAGCCATTGGCGCATTAAGACTCATCTCGCTATCAGCCATCTAGATGTCAGCAAAATTGGTAATATTGTAGCTGATATGCGGAAAGTCTTAGCAAAAAATCATCAAGTGGAGCAACAACGGTTGCATCGAAGAGTATTTCTGGATAATATCGATCCAGAAAATCAGGCACTTTTGATTTTGGTTTCTTGCTTTGTAAAGACCCCCCACTTTGAAGAGTATCTTTGTGTTAAGGAAGCCATTTTATTGGACCTTCTAAGAGTTATCAGTCATCATCGTGCCCGATTGGCTACTCCAATCCGTACTGTACAGAAAGTATATGGTGACAGCGAGACACAGAATATACCATTTGAGGAAACCATAATCAGAAGAGGAGTGCCTAAGATGATTGAAAGTCCAAAATTAACTGTCGAGGATAAAGTAAAACAACGTTCTCTTCGTAGCAAGGAAGAGTTGAATACTGTTCATCCAAAGGCAGTGTCTGTAGAGGCTAAAGGGACTGAACCTGGAGTTTCTTCCAATGAGAATACAAAACCTAGTAGCTTGGATGGAAAGAATATTCACAAGCAGAAAGAGAAAGAGAAAGATAGGGACCATGCTCCTTCAGACTTGCAACCAAAGATGCTAGATGCAGTTCAAACATCACATACAAAGCCATCTCTTGAAGGTTTAGATTCAATGGGACTGAATTCCAAGGACATCACATTGCTTAGGGCAGCATTTGAGAAACCTCCAGTTATTATTCCTGAAGCACCCAGGGATCCTGTAATTCCAAAGAATGAGAATGGAAATAATGAGAAGAACAATGCAAGGAGTGAGAAGAATGCTACAACTAATGAGAAGTCAAACTCACAAGTTTTTGAAAAGCAATCATATCAAAATATTAAGGGAGGTGAAAAGAATGGGAGCAAGCTATCTATTGAAGTTGATAGTTCAACCATAGAAATAAATAAAGGTAAGGCCAACTCTGTAGGTGAATTGGAAATCGAACTAAGACCTGAGAAAAACAAACAAAAAGGCCAGGGGTCTTCCTCTGTACCATCTTCCAAGCATGAGAATGAACGATCTGTTAATTCTGCCTCAATCTCAAGACCTCTGCTGAAACAAAATTTACCTCTGGGTGTTGCTCTGGACGGGCCAAAAAGAACTCTTCCAATTGAGGGAGAAATTACTTCACAAGTGGAGCAGAAAGAACTTGCACCATTGCGAAATGGTAATAGTAGTTCTGGAGTCAAGGATAGGAGGGAACCTCAGTCACCATCCTCAAGCTCTGTTCATTCTGATGAAAAAGATCAGTAGAGGTGATGACAGTAGTATCTGGTAACTTATAGAACCCTGGCATGTTTGTTAATTTACTAACTAATCCATCTTGTTAGGATGCCATAGAATGGGCAGAATAAAAGGGTAGTATCAGAGTTTTCTGAATGTCTTTTGAATCTGATCACTATATAGTATTCTTATATATTATATAGATTTGTAATATAAAGTATAATGATATTACTTGGGTCCAAAAATCAACGAATTAACTTACCCAAGCCTTT

Fln Alignment:

Contig27731___DRPCMLSHCLSSCAPRQDAWSLKMSKRVNMPLCTVPTFRAVVTCKSLFSSGQLNHALLLSIKPAALSLTRAYNALQGSPLVLQCAPAVGLMAFSVWGLEPSLQVIRHLILRRNANNWKRSSTYHIMTSYLQPILLWLGAILICRAFDPVVLSSETSQAVKQRLVNFVRSLSTVLAFAYCISSLIQQAQKFVMEHNDTQDTRHVGFNFIGKAIYTAVWVAAVSLFMELLGFSTQKWIXXXXXXXXXXXXXGREIFTNFLSSVMIHATRPFVVSEWIQTKIDGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVSVVRNLTQKSHWRIKTHLAISHLDVSKIGNIVADMRKVLAKNHQVEQQRLHRRVFLDNIDPENQALLILVSCFVKTPHFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKVYG--DSETQNIPFEETIIRRGVPKMIESPKLTVEDKVKQRSLRSKEELNTVHPKAVSVEAKGTEPGVSSNENTKPSSLDGKNIHKQKEKEKDRDHAPSDLQPKMLDAVQTSHTKPSLEGLDSMGLNSKDITLLRAAFEKPPVIIPEAPRDPVIPXXXXXXXXXXNARSEKNATTNEKSNSQVFEKQSYQNIK---GGEKNGSKLSIEVDSSTIEINKGKANSVGELEIELRPEKNKQKGQGSSSVPSSKHENERSVNSASISRPLLKQNLPLGVALDGPKRTLPIEGE----ITSQVEQKELAPLRNGN-SSSGVKDRREPQ
F6H132_______________DRLHLLSITLSSHASRQDALSLHLLNSMRGSITPVSSRCEFFLCRSFLAPGGGNEISVL--KSAALVLTRSCNALRGRPLVLQLVPAVSIVAFAAWGLGPLMRLSRNLFLNKTDSSWKKSSTYYVLTYYLQPLLLWIGAMLICRALDPIILPSKESQAVKQRLLIFIRSLSTVLASAYCLSSLIQQVQNFFMENNDSSDARTMGFQFAGKAVYTAIWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLDYIDPENQALLILVSCFVKTPRFEEYLCVKEAILLDLLRVISHHQARLATPIRTVQKEYSMADMEMENIPFADPIFTRS-SAAANRPLLLIEPSYK----------------------------MNGDDKTKASTGSAC-----QNEEKDAN----------IDASSTSESKPDAKA----GASS---------------ILDSTTDDNVA------------ATSISNSSTNSKVSATSISDPKIQNMVTDGSTQNNYEEQQSEASMEKVREDINPGGSAFEKPSLNFPESGAGKADG---LPSATPLAKQDGNRASIATPALEENIVLGVALEGSKRTLPIEEEEMVVSPSGAESKELAACQNGNVSAPNGKDKKEGQ

SNPs (tot: 52)

Position	A %	C %	G %	T %	Probability
1620	75		25		1.0
1681	10			90	0.90298
1682		10		90	0.90298
1683	10		90		0.90298
1686		10		90	0.90298
1687		10		90	0.90298
1688	10		90		0.90298
1690	90		10		0.90298
1691			10	90	0.90298
1692		90		10	0.90298
1697		90	10		0.90298
1700			10	90	0.90298
1708	88			11	0.923564
1709	88		11		0.923564
1712			90	10	0.90298
1714	10	90			0.90298
1715		10	90		0.90298
1720		10	90		0.90298
1723	10			90	0.90298
1730		10		90	0.90298
1731			10	90	0.90298
1753	85		14		0.993533
1755		14		85	0.993533
1757			14	85	0.993533
1762	14	85			0.993533
1764	85		14		0.993533
1765		85		14	0.993533
1768		14	85		0.993533
1769		85	14		0.993533
1771	85	14			0.993533
1824	85	14			0.993533
1826	85			14	0.993533
1827		85		14	0.993533
1884	14			85	0.993533
1885	14			85	0.993533
1887			14	85	0.993533
1888		85		14	0.993533
1898		12		87	0.992554
1899	87	12			0.992554
1900	87	12			0.992554
1932	16		83		0.994512
1997		80		20	0.995493
2299	20			80	0.995493
2304		80		20	0.995493
2328		33		66	0.997452
2349	66	33			0.997452
2389	66	33			0.997452
2391	66	33			0.997452
2393		66		33	0.997452
2478		33		66	0.997452
2497	66	33			0.997452
2498	66	33			0.997452

SSRs (tot: 1)

Start position	End position	Sequence	Length
955	973	TCTTTC TCTTTC TCTTTC T	19

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain