SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27656

Length: 1993 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene27656
AGATAAGCCTCCAGCCTTTGAAGAGAACTTACAAGTGCAGCTCCTGAATTTCAACTAAGAGGCTCAATCCTGAATTTAGAACTGTATAAAACAAGGTCCAGCATGGCTAGTGTCATAACAAATCCAACCTCAGCACTTCAGTCTCTTGTGGATAAAGTTGAGGGAAGGTTTGAAAGCCAGTGCTGTATTGGGGTTGGACCTGGCTATGCAAGTCCTAAAGAAGCAATGAAAGGTCCTCGAGAGGCGCTTCTTTATGTTACAGCTGTATATACAGGAACTGGAAGAGAGAAGCCGGATTACTTGGCTACAGTAGATGTAGATCCAACTTCACCAACTTATTCAAAGGTTGTGCATCGCTTGCCCGTAACTAATTTTGGTGATGAGTTGCATCATTCTGGCTGGAATGCATGTAGCTCTTGCCATGGGGATGCTTCTGCTCAAAGGCGTTATCTCATCTTACCTTCATTGGTATCAGGTCGTATTTATGCTATTGATACTGCCAAGGATCCACGAGCTCCAGTCATTTACAAGACTGTGGAGCCAGAAGTTATCTTGTCCAAGACTGGGTTGGCATATCCTCACACTGCTCATTGCCTTGCATCTGGTGATATTTTAATCTCTTGCCTTGGAGGCAAAAATGGGAATGCAGAGGGCAATGGATTTCTGCTTTTAGACTCAGATTTAAATGTTAAAGGAAGGTGGGAAAAACCAGGTGACAATCCTAAGTTTGGCTATGACTTTTGGTACCAACCACGACACAAGACAATGATTAGTTCATCCTGGGGGGCACCTGCAGCTTTTACAAAAGGATTCAATCCTCAACATGTTGCGGAAGGCTTATATGGCAAGCACTTATTTGTGTATAGTTGGCCTGATGGTACATTAAAGCAAACAATTGACCTGGGTAATGAAGGACTCATACCACTGGAGGTAAGATTTCTCCATGACCCATCAAAAGATACAGGCTATGTAGGATGTGCTTTAAGTGGCAATATGGTGCGTTTTTTCAAGACAACAGATGGATCATGGGACCATGAGGTTGTAATTTCAGTACCAGCACTTAAGGTACAAAACTGGGTCCTGCCAGAAATGCCTGGCTTGATAACTGATCTTCTTATATCCTTGGATGATAGATATCTTTATTTTGTAAATTGGCTGCATGGGGACATTCGACAATACAACATCGAAGATCCTAAGAAGCCTGTGTTGGTTGGCCAAGTGTGGGTGGGGGGTTTAATTAGAAAAGGAAGCGAAGTTACGGTTGAAAAAGAAAATGGACAACAATGGCAGTCTGATGTTCCAGATGTTCAAGGTAAGCGTTTGAGGGGCGGACCACAGATGATTCAGCTGAGTTTGGATGGGAAACGACTTTACGTGACAAACTCTCTGTTTAGTGCCTGGGACCGTCAGTTCTACCCGGAACTTATCGAGAAAGGCTCTCATATGTTGCAGATTGATTGCGATACAGAGAAGGGCGGGCTTGCTGTAAATGCTAACTTCTTTGTAGATTTTGAAACTGAACCAGATGGACCTGCTCTTGCTCATGAAATGAGGTATCCAGGAGGTGACTGCACTTCAGATATATGGGTCTGAAAATAAGAGAATGCTGAGCTCCTAATGGGTCGCTATATTCTCTGAAAATATATCTAGATGTTTTCTCTTATAAATCCAAGTTGATGCTCATTTGAATAAGTATCTGTTTTGAACGAGAATTGCAAACGATGCACTTTATTTTATATCGGAGCTGAACTAAGTTATGCATTAGTAAATGTGTCGTGTTACTTTTAAATTTACGGAACACATTATGACAGTGCAAATTATAAAGTAATCTCGCGAATCCGTCCTAAAATTAGCTGTTGAATATCTCAAAATAAAAGGACCCAAAATATTATATTCCAACCTGTAACATGAAAACATGTTTTTACCTAAATCTAGTATATGGAAGTTGGTTGTTCTGAAATTCCTGTCAGCAACAACCGAGCTTGCTTTGTCT

Ace file of the UniGene sp_v2.0_unigene27656 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	selenium binding protein [Medicago sativa]	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	selenium-binding protein	-	-	87%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	87%
Blast2go	cellular response to selenium ion	GO:0071291	Biological Process	87%
Blast2go	sulfate assimilation	GO:0000103	Biological Process	87%
Blast2go	response to hydrogen peroxide	GO:0042542	Biological Process	87%

Source	InterPros	Term	Type	e value	Identity
Sma3	Selenium-binding protein	IPR008826	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8J6

Fln msg:

Fln protein: MASVITNPTSALQSLVDKVEGRFESQCCIGVGPGYASPKEAMKGPREALLYVTAVYTGTGREKPDYLATVDVDPTSPTYSKVVHRLPVTNFGDELHHSGWNACSSCHGDASAQRRYLILPSLVSGRIYAIDTAKDPRAPVIYKTVEPEVILSKTGLAYPHTAHCLASGDILISCLGGKNGNAEGNGFLLLDSDLNVKGRWEKPGDNPKFGYDFWYQPRHKTMISSSWGAPAAFTKGFNPQHVAEGLYGKHLFVYSWPDGTLKQTIDLGNEGLIPLEVRFLHDPSKDTGYVGCALSGNMVRFFKTTDGSWDHEVVISVPALKVQNWVLPEMPGLITDLLISLDDRYLYFVNWLHGDIRQYNIEDPKKPVLVGQVWVGGLIRKGSEVTVEKENGQQWQSDVPDVQGKRLRGGPQMIQLSLDGKRLYVTNSLFSAWDRQFYPELIEKGSHMLQIDCDTEKGGLAVNANFFVDFETEPDGPALAHEMRYPGGDCTSDIWV*

Protein Length: 498

Fln nts: AGATAAGCCTCCAGCCTTTGAAGAGAACTTACAAGTGCAGCTCCTGAATTTCAACTAAGAGGCTCAATCCTGAATTTAGAACTGTATAAAACAAGGTCCAGC_-_ATGGCTAGTGTCATAACAAATCCAACCTCAGCACTTCAGTCTCTTGTGGATAAAGTTGAGGGAAGGTTTGAAAGCCAGTGCTGTATTGGGGTTGGACCTGGCTATGCAAGTCCTAAAGAAGCAATGAAAGGTCCTCGAGAGGCGCTTCTTTATGTTACAGCTGTATATACAGGAACTGGAAGAGAGAAGCCGGATTACTTGGCTACAGTAGATGTAGATCCAACTTCACCAACTTATTCAAAGGTTGTGCATCGCTTGCCCGTAACTAATTTTGGTGATGAGTTGCATCATTCTGGCTGGAATGCATGTAGCTCTTGCCATGGGGATGCTTCTGCTCAAAGGCGTTATCTCATCTTACCTTCATTGGTATCAGGTCGTATTTATGCTATTGATACTGCCAAGGATCCACGAGCTCCAGTCATTTACAAGACTGTGGAGCCAGAAGTTATCTTGTCCAAGACTGGGTTGGCATATCCTCACACTGCTCATTGCCTTGCATCTGGTGATATTTTAATCTCTTGCCTTGGAGGCAAAAATGGGAATGCAGAGGGCAATGGATTTCTGCTTTTAGACTCAGATTTAAATGTTAAAGGAAGGTGGGAAAAACCAGGTGACAATCCTAAGTTTGGCTATGACTTTTGGTACCAACCACGACACAAGACAATGATTAGTTCATCCTGGGGGGCACCTGCAGCTTTTACAAAAGGATTCAATCCTCAACATGTTGCGGAAGGCTTATATGGCAAGCACTTATTTGTGTATAGTTGGCCTGATGGTACATTAAAGCAAACAATTGACCTGGGTAATGAAGGACTCATACCACTGGAGGTAAGATTTCTCCATGACCCATCAAAAGATACAGGCTATGTAGGATGTGCTTTAAGTGGCAATATGGTGCGTTTTTTCAAGACAACAGATGGATCATGGGACCATGAGGTTGTAATTTCAGTACCAGCACTTAAGGTACAAAACTGGGTCCTGCCAGAAATGCCTGGCTTGATAACTGATCTTCTTATATCCTTGGATGATAGATATCTTTATTTTGTAAATTGGCTGCATGGGGACATTCGACAATACAACATCGAAGATCCTAAGAAGCCTGTGTTGGTTGGCCAAGTGTGGGTGGGGGGTTTAATTAGAAAAGGAAGCGAAGTTACGGTTGAAAAAGAAAATGGACAACAATGGCAGTCTGATGTTCCAGATGTTCAAGGTAAGCGTTTGAGGGGCGGACCACAGATGATTCAGCTGAGTTTGGATGGGAAACGACTTTACGTGACAAACTCTCTGTTTAGTGCCTGGGACCGTCAGTTCTACCCGGAACTTATCGAGAAAGGCTCTCATATGTTGCAGATTGATTGCGATACAGAGAAGGGCGGGCTTGCTGTAAATGCTAACTTCTTTGTAGATTTTGAAACTGAACCAGATGGACCTGCTCTTGCTCATGAAATGAGGTATCCAGGAGGTGACTGCACTTCAGATATATGGGTCTGA___AAATAAGAGAATGCTGAGCTCCTAATGGGTCGCTATATTCTCTGAAAATATATCTAGATGTTTTCTCTTATAAATCCAAGTTGATGCTCATTTGAATAAGTATCTGTTTTGAACGAGAATTGCAAACGATGCACTTTATTTTATATCGGAGCTGAACTAAGTTATGCATTAGTAAATGTGTCGTGTTACTTTTAAATTTACGGAACACATTATGACAGTGCAAATTATAAAGTAATCTCGCGAATCCGTCCTAAAATTAGCTGTTGAATATCTCAAAATAAAAGGACCCAAAATATTATATTCCAACCTGTAACATGAAAACATGTTTTTACCTAAATCTAGTATATGGAAGTTGGTTGTTCTGAAATTCCTGTCAGCAACAACCGAGCTTGCTTTGTCT

Fln Alignment:

Contig27656___MASVITNPTSALQSLVDKVEGRFESQCCIGVGPGYASPKEAMKGPREALLYVTAVYTGTGREKPDYLATVDVDPTSPTYSKVVHRLPVTNFGDELHHSGWNACSSCHGDASAQRRYLILPSLVSGRIYAIDTAKDPRAPVIYKTVEPEVILSKTGLAYPHTAHCLASGDILISCLGGKNGNAEGNGFLLLDSDLNVKGRWEKPGDNPKFGYDFWYQPRHKTMISSSWGAPAAFTKGFNPQHVAEGLYGKHLFVYSWPDGTLKQTIDLGNEGLIPLEVRFLHDPSKDTGYVGCALSGNMVRFFKTTDGSWDHEVVISVPALKVQNWVLPEMPGLITDLLISLDDRYLYFVNWLHGDIRQYNIEDPKKPVLVGQVWVGGLIRKGSEVTVEKENGQQWQSDVPDVQGKRLRGGPQMIQLSLDGKRLYVTNSLFSAWDRQFYPELIEKGSHMLQIDCDTEKGGLAVNANFFVDFETEPDGPALAHEMRYPGGDCTSDIWV
D5A8J6_______________MASVITNATSALQSLVDKVEERFQNQCCIGAGPGYASPKEAMEGPREALLYVTSIYTGTGRGKPDYLATVDVDPTSPTYSKVVHRLPVTHFGDELHHSGWNACSSCHGNASAQRRYLILPSLVSGRIYAIDTAKDPRAPVLYKVVEPETILSKTGLAYPHTAHCLASGDILISCLGDKDGNAEGNGFLLLDSDLNVKGRWEKPGDSPMFGYDFWYQPRHKTMISSSWGAPAAFTKGFNPQHVLDGLYGKHLFVYSWPDGTLKQTIDLGNEGLIPLEVRFLHDPSKDTGYVGCALSGNMVRSFKTSDGSWDHEVVISVPPFKVQNWILPELPGLITDLLISLDDRYLYFVNWLHGDIRQYNIEDPEKPVLVGQVWVGGLVRKGSKVIVEKENGQQWQSDVSDVQGKRLRGGPQMIQLSLDGKRLYVTNSLFSAWDRQFYPELIEKGSHMLQIDCDTEKGGLAVNANFFVDFETEPEGPALAHEMRYPGGDCTSDIWV

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
1398	90		10		0.90298
1434		10		90	0.90298
1633		20	80		0.995493
1634		80	20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain