SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27601

Length: 1415 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene27601 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DUF3844 domain containing protein	-	-	3.0e-05	31%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	63%
Blast2go	protein	-	-	0.0	65%

Source	InterPros	Term	Type	e value	Identity
Sma3	EGF-like region, conserved site	IPR013032	-	0.0	-
Sma3	Beta-ketoacyl synthase, active site	IPR018201	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQW8

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 302 - 323

Fln protein: MDRHLSICAIFLVISCFSFPQRSDGAACRDLCGNVPVKFPFGTGPGCGDPRFQSYVRCVNQKLMLTTHSGTYQITSIDYDNHVILITDPLMSTCTSMQNSGSFGLDWSAPFKIHNDVFVLLGCSSSSSLFDAQKPLCDTGSSHICSSLYSCSGITGLGITPNSPISTCCVYSPINLGPADELNLPKLQCSSYSSIYNFGDDPTNPKRWKYGIALQYSYSLYNNYFTTSCNDCEQSSGVCGYTGASNSFVCICKNGVNTTTNCYGQGYVWSADAGQRKWSLEHWVTTGVLLLVGSLLLLFKI*

Protein Length: 303

Fln nts: AAGAATAAAGTCCCAGACGCCGAAAGGGATAAGGATTGAAGTTTAATATATTGATCTCGACAATTGCATCTCTGAGTGCTCACTTCTAGTTTTCTTGTTCAGAAAGCCGATCAAGAAA_-_ATGGATCGACATTTGTCCATCTGTGCAATTTTTCTTGTGATTTCTTGCTTTAGTTTCCCCCAAAGAAGCGACGGTGCTGCGTGCAGAGACCTGTGCGGGAACGTCCCTGTCAAATTCCCCTTCGGAACAGGGCCGGGCTGCGGAGACCCCAGGTTCCAGAGCTACGTCCGCTGCGTGAACCAGAAGCTCATGCTGACCACACATTCCGGCACTTACCAGATAACCTCCATAGATTACGACAACCATGTCATTCTCATCACCGATCCCCTAATGTCCACCTGCACTTCGATGCAGAACAGCGGCAGCTTCGGCTTGGACTGGTCCGCGCCTTTCAAGATCCACAACGATGTGTTTGTTCTTCTGGGATGCTCTTCTTCCTCTTCCCTCTTTGACGCTCAGAAGCCTCTCTGCGACACAGGAAGTTCGCACATCTGTAGCAGTTTGTACAGTTGCTCAGGCATCACAGGACTCGGGATTACCCCCAATTCCCCCATATCGACCTGCTGCGTGTATTCTCCCATCAATCTTGGACCAGCAGATGAGCTCAATCTCCCCAAACTTCAGTGCTCGTCGTATTCGAGTATTTACAACTTTGGGGACGATCCCACTAATCCCAAGCGGTGGAAATACGGAATAGCGCTTCAGTACAGTTACAGTCTCTACAATAACTACTTTACTACTTCCTGTAACGACTGTGAACAGAGCAGCGGCGTATGCGGTTACACGGGGGCTTCCAATTCATTTGTCTGTATCTGTAAAAATGGAGTTAATACTACTACAAACTGCTATGGACAAGGATACGTTTGGAGTGCAGATGCAGGTCAAAGAAAATGGAGTCTGGAGCACTGGGTGACCACAGGCGTTCTGCTTCTGGTTGGCTCTCTGCTTCTCCTCTTCAAGATTTAACCAACGCTGCTGTCGAATGGCAACTATTCTTCCTTCAAGCCAGTTACTCAGTATGTGTTTCTGTGACCCAAAAACTAAATGTATGTAACAATTCAATGGATACTCCGTTTGAGAGGATCTGTGTAGTTGAGGAGGAGCGATTGTTTGGTTACGCATATGATTTTTCATAATGATATTTTAGCCACTGTGTGTTTCACCTGTAATAACAATATGTCACAGCGAGGAACTGAAAAGTTAATTGTAAATGAGAAAGGTATTGGTTCATAGACAATGGCAGGGCTGGTCATTCTGGCCTCTGATTTTCATTTTAGTGTATCATGTATTATTTGTAGAGTATTAATCTACAGTAATATTAACCGTCTTTATTTGTGTCCTAAAGTCAATATTTTAG

Fln Alignment:

Contig27601___LSICAIFLVISCFSFPQRSDGAACRDLCGNVPVKFPFGTGPGCGDPRFQSYVRCVNQKLMLTTHSGTYQITSIDYDNHVILITDPLMSTCTSMQNSGSFGLDWSAPFKIHNDVFVLLGCSSSSSLFDAQKPLCDTGXXXXXXXXXXXSGITGLGITPNSPISTCCVYSPINLGPADELNLPKLQCSSYSSIYNFGDDPTNPKRWKYGIALQYSYSLYNNYFTTSCNDCEQSSGVCGYTGASNSFVCICKNGVNTTTNCYGQGYVWSADAGQRKWSLEHW
B8LQW8_______________LSLCCLFLVPS-------AAGWACRDTCGNLQVKYPFGTGPGCGDPRFQNSVSCVNQKLMFTTHSGSYPISSIDYSQTTIYLADPQMSTCAAMTSSGSFGLDAAAPFKFKNDIFVLLNCSLSSCLYSPTNYLCDTGSSQICSSLYTCPGVLGLGLQQYSPISTCCVYAPINLGSAAEINLAKLQCQSYTSIYSFGDDATDPTRWNYGIALKYSFNLDNSNFPTACVSCEQSKGVCGFTGMYNSFTCVCRNGVNTTTNCNGQEYYWSRAQSHRSAAFGLW

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
484	10			90	0.90298
488		90		10	0.90298
489	40		60		1.0
507		11		88	0.923564
525	22		77		0.999999
572		90	10		0.90298
639		10	90		0.90298
651	10		90		0.90298
681	10	90			0.90298
682	10			90	0.90298
685			88	11	0.923564
693	10		90		0.90298
711		11	88		0.923564
712		11		88	0.923564
713	88	11			0.923564
715	88			11	0.923564
738	87		12		0.992554
804			12	87	0.992554
807	16		83		0.994512
873		25	75		0.996473
959	20		80		0.995493
1342		13		80	0.999961
1344	64	35			1.0
1345	21	14	64		1.0
1346	64	14		21	1.0
1347		18	81		0.999994
1359		11	88		0.923564
1385		20		80	0.995493
1386		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain