SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27022

Length: 1507 nt

Origin of reads: Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene27022

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase (Fragment) n=1 Tax=Musa acuminata AAA Group RepID=C4N552_MUSAC	-	-	78%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	38%
Blast2go	probable pectinesterase pectinesterase inhibitor 41-like	-	-	80%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Twin-arginine translocation pathway, signal sequence	IPR006311	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Carbohydrate-binding/sugar hydrolysis domain	IPR006633	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PT44

Fln msg: Separated hits, possible frame ERROR between 419 and 430, and overlapping frame ERROR between 603 and 593, query STOP codon is far from subject stop. Distance to subject end: 20 aas, your sequence is shorter than subject: 408 - 601

Fln protein: QATCLQSLEEASGSLQNILTESVLNMRRVVSNSLALVKKISVVGKNSKRPHVHSRRLLSNPTSHEPHDQVDVDEDKFLSHYGVVNDGFPSWLSASDRRRLLQTSEENDGALAENVVTVAQDGSGNYSTITDALKVAPKxxxxxRYVIYVTEGVYQEYIEVPKYKTYIMLVGAGINSTVITGNRSVVDGWTTYRSATxxxxxxQGFIARDITFENTAGPEKHQAVALRVASDLSAFYRCSFKGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAAVFQNCNLLARKPMDNQKNCFTAQGRDDPNQNTGISIQKCNVSPAPDLAPVKSSFETFLGRPWKKLLSHSLSRVTYR*CGRSCRLARMVGGLCLDDALLWRIYELGTRSRNCGKSSMAWVSHHEHF*CSKFHSD*

Protein Length: 409

Fln nts: ATCAGGCTACCTGCCTCCAGAGTCTTGAGGAGGCTAGCGGAAGCCTGCAAAACATTTTGACTGAGAGTGTGCTTAATATGCGGCGTGTTGTGAGCAATTCATTGGCTCTTGTCAAAAAGATTTCTGTCGTCGGTAAAAATTCCAAGAGACCTCATGTGCACAGCCGACGCCTGTTATCCAATCCAACGAGTCATGAGCCTCACGATCAAGTTGATGTAGATGAAGATAAATTTCTTTCACATTATGGAGTCGTCAATGATGGATTCCCTTCATGGTTGTCTGCTTCAGACCGAAGGAGACTTCTTCAAACCTCTGAAGAAAATGATGGCGCTTTGGCCGAGAATGTCGTCACCGTGGCTCAAGATGGTAGTGGTAATTATTCCACAATTACTGACGCGCTAAAGGTTGCTCCAAAAAAxxxxxxxxxxxxTAGATATGTGATATATGTTACAGAGGGAGTCTACCAGGAATACATTGAGGTGCCCAAATACAAAACATATATTATGTTGGTGGGAGCTGGCATAAATTCCACTGTGATCACCGGAAATAGAAGCGTTGTGGACGGATGGACTACTTATCGCTCTGCAACAGxxxxxxxxxxxxxxxGGCAAGGCTTTATCGCTCGGGATATCACTTTCGAGAATACGGCGGGCCCAGAGAAGCATCAGGCTGTCGCTCTCCGCGTTGCCTCGGATCTATCAGCGTTCTACCGCTGCAGCTTCAAAGGTTACCAAGACACTCTTTACGCCCACTCCCTTCGGCAGTTCTACAGGGAATGCGACATTTATGGCACAGTTGATTTTATCTTCGGCAACGCTGCAGCTGTGTTTCAAAACTGCAATCTACTGGCTAGGAAACCGATGGATAATCAAAAGAATTGTTTTACAGCTCAAGGAAGAGATGATCCAAATCAAAACACTGGAATCTCTATTCAAAAGTGCAACGTCTCTCCTGCGCCTGATCTCGCTCCAGTGAAGAGCTCTTTCGAAACATTCTTGGGAAGGCCATGGAAAAAATTACTCTCGCACAGTTTATCTCGAGTCACATATAGATGATGTGGTCGATCCTGCAGGTTGGCTAGAATGGTCGGGGGACTTTGCCTTGACGACGCTCTATTATGGCGAATATATGAACTCGGGACCCGGAGCAGGAACTGCGGCAAGAGTTCAATGGCCTGGGTATCACACCATGAACATTTCTGATGCTCAAAATTTCACAGTGACTAA___TTTCATTGCTGGTGATGACTGGATACGAGAGGTGCCCTTCGATGCAGCCTTAGATTAACACGAATGACAGTAATTTTTTGTACTGTCACTAAGAGCTGCTTGGATGGATTGAAACAAAACTCAGAGGCAGCAAGACTAGCATTGGAATTATCTCCGTTCATTCTTTATTAATTTCAACTGTAACATTCTTTATTAATTCAACTGTAGAAATCTTTATTGATTTCAACTGCAGCAAGTGTAAATAATCTACGTAATGGACTTTACAATTTCAGTCTATAAATTCAT

Fln Alignment:

Contig27022___QATCLQSLEEASGSLQNILTESVLNMRRVVSNSLALVKKISVVGKNSKRPHVHSRRLLSNPTSHEPHDQVDVDEDKFLSHYGVVNDGFPSWLSASDRRRLLQTSEENDGALAENVVTVAQDGSGNYSTITDALKVAPKKxxxxRYVIYVTEGVYQEYIEVPKYKTYIMLVGAGINSTVITGNRSVVDGWTTYRSATVxxxxQGFIARDITFENTAGPEKHQAVALRVASDLSAFYRCSFKGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAAVFQNCNLLARKPMDNQKNCFTAQGRDDPNQNTGISIQKCNVSPAPDLAPVKSSFETFLGRPWKK
C0PT44_______________QYTCLDS--SARSNLRQELQGGLMSISHLVSNSLAIVKNIATRASNVTVNSIHNRRLLS--------------DDQGSEFMAMESDGFPSWMSAKE-RSLLQSSRDN---IMPNAV-VAKDGSGHHTSIGDAVNAAPQKxxxxRYVIHIKAGIYWENVEVNKKKTHLMFIGDGIGATVVAGNRNVKDGYTTYRSATVxxxxNGFIARDITFENTAGAAKHQAVALRVGSDFSAFYRCSFQGYQDTLYVHSLRQFYRECNVYGTVDFIFGNAAVVLQNCNLFARKPLANQQIVYTAQGRQDPNENTGISIQNCQVIAASDLIPVKRSFPAYLGRPWRQ

SNPs (tot: 1)

Position	A %	C %	G %	T %	Probability
1310	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain