SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene17

Length: 2489 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene17
TGGTACAACATGGTCACTAGAGAGGTTCATTCAGCTGTTTCAGTCGACATTGAAATCACATGCCAAGGACGTTGTTGCCTTTAAGAGTATAGCTGCTTATCGCAGTGGTCTTAATATAAATCCATTGGTGAAAAGTCAAGATGCAGAAGATGGTCTTTACAAAGATTTATGCGCCAGGAAGCCTGTACGCATTAGTAACAAAAGCTTCATTGACTACATTTTTACTCTCAGCTTGGAAGTGGCCACATCTTTTGATTGTCCCATGCAAATACATACAGGTTTTGGAGACAAGGACCTGGACCTGAGGCTTGCAAACCCTCTTCATCTTCGGCCAATCTTGGAAGATGATCGATTCAAAAAATCTCGCATTGTGCTTCTTCATGCGTCCTATCCCTACTCAAAGGAAGCATCTTATTTGGCTTCTGTTTATCCACAGATCTACTTGGACTGTGGCCTAGCAATACCAAAGCTCAGTGTTCATGGGATGTTCTCTGCAGTTAAGGATCTGTTAGAACTGTGCCCACTAAACAAGGTGATGTTTAGCACAGATGGATATGCATTTCCCGAAACATATTATCTTGCTGCAAAATGGGCACGCAAAGTAGTTGTATCTGTTCTTTCTGATGCTTGTGATGGTGGAGATCTCTCTATTCAAGAAGCTATTGAAGCCGCACAGAATATCTTACGGGACAATGCAGTCAGTTTCTATAAGCTGAATGAAAATAGTACACAAAATGTGATATCTGAAGTGGGTACCTCAGTGACTAATTCTCAGATTCTGGATCTAGAAGGTAATGAAGGAAAACCTAAGTTTGTTCGTCTCCTTTGGGTTGATACTTCAGGTCAGCAAAGATGTCGAGTTGTTCCAGAAAATCGTTTCAACAAAGTAGTGAAGTATCATGGAGTGGGTTTAACATTTGCGTGCATGGGTATGACTTCAAGCTCAGATGGTCCAGCTGAAGGTTCCAATCTTACTGGTGTTGGTGAAATCCGACTGATGCCTGACATGAAGACACTGTGCAGTTTGCCCTGGTCTGACCAAGAGAAGATGGTATTAGTTGATATGCATTTGAAACCTAGCCATCCTTGGGAATATTGCCCAAGGACAGCCTTACTAAGAGTTACAATGACATTGGAAGAAGAATTTGGCTTGGTTGTGAATGCTGGTTTTGAGAGTGAGTTCTACATTTTGAGGAAAACAACGAGGGAAGTTAATAACAAGTGGGTGCCCATTGACTCGACCCTATACTGCTCAACTGTAGCATTTGATGCTGCAACTCCTGTTCTATTCGAAGTTATTAAAGCTCTCCAAAAGATGCACATATCTGTAGAACAGGTGCATGCAGAATCAGGGAATGGCCAGTTTGAAATATCCTTGGGGCATTCACCTTGCATGGAGGCAGCTGATAACTTAGTTTTCGTAAAAGAGACTATAAGGGCAATAGCAAACAGAAATGGACTTCTTGCGACGTTTATACCCAAGTATGCTTCAAATGACGTTGGTTCAGGATGCCATGTACATTTAAGTTTATGGCAAAACGGCAAGAACAAGTTTATGGCTGAAGATGGGTCAATTACTAAGCATGGTATGTCCACAATCGGGGAAGAGTTTATGGCTGGAGTCTTCCATCATCTTCCGTCCATATTGGCCTTCACAGCTCCATTGCCAAACAGTTATGAGCGATTGAAGCCTAACACATGGAGTGGAGCATATCATTGTTGGGGGAAGGAAAATCGAGAAGCTCCAATCCGAACAGCATGTCCTCCTGGGATTTCAAGTGAAGTCGTGAGCAACTTTGAGATTAAAGCATTTGATGGTTGTGCTAATCCATATCTGGGACTAGCAGCAGTTATAGCTGCTGGAATAGACGGCCTGCGGAAGCACCTTGTTTTGCCAGAGCCTGTTGACACAAACCCGGACTATCTCAAGGAAGGTGCGGTGCACAGATTGCCCACAACGTTGGATGAGTCTGTTGCAGAACTCAGAAAGAATGAAGTCCTGAAGAACCTTATTGGCGATAAACTCATGAATGCAGTAATTGCTGTCCGCGAGGCTGAAATTCGATATTATTCCGCCACCTTAGACAATGGTGTGGAGAAATTGATCCATAGGTATTGAAGTTCAAATTTATGACAAAATGATGTGATAGCATTCTGGGAATTATAACATACTATATGTTATCTAACAAAGTGTGTGGTATTTTGAATTGTAATATTTTTGAAGACATCAAAGGGTACCTTATATGTTATCTAACAAAGTGTGTGGTATTTTGAATTGTTTTATTTTTGAAGACATCAAAGGGTACCTTAAATCACATCACTAACAGTTATTTCTTACCATTCTAATTATTGCACGTTATAATGAGTTTGTTAACGAGTATTGAGTGGTTAGTTAGCAATCCCATGGAAGATGCATGTAATTATTATATATTTTGTGAAAGTATTGCAAAATAAAGCGCTCGAGTAAGACTGGGATTGTTTTCAACATT

Ace file of the UniGene sp_v2.0_unigene17 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	glutamate-ammonia ligase-like protein [Arabidopsis thaliana] gb\|AEE79045.1\| glutamate-ammonia ligase-like protein [Arabidopsis thaliana]	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	63%
Blast2go	protein	-	-	73%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate--ammonia ligase.	EC:6.3.1.2	-	0.0	73%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	73%
Blast2go	Arginine and proline metabolism	00330	73%
Blast2go	Nitrogen metabolism	00910	73%
Blast2go	Metabolic pathways	01100	73%

Source	GOs	Term	Type	Identity
Blast2go	cellular biosynthetic process	GO:0044249	Biological Process	73%
Blast2go	primary metabolic process	GO:0044238	Biological Process	73%
Blast2go	nitrogen fixation	GO:0009399	Biological Process	73%
Blast2go	cellular nitrogen compound metabolic process	GO:0034641	Biological Process	73%
Blast2go	response to abscisic acid stimulus	GO:0009737	Biological Process	73%
Blast2go	glutamate-ammonia ligase activity	GO:0004356	Molecular Function	73%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	73%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	Amidohydrolase 2	IPR006992	-	-
Sma3	Glutamine synthetase, catalytic domain	IPR008146	-	-
Sma3	Glutamine synthetase/guanido kinase, catalytic domain	IPR014746	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: tr_plants

Fln subject: D7TIU8

Fln msg: your sequence is shorter than subject: 706 - 840

Fln protein: GTTWSLERFIQLFQSTLKSHAKDVVAFKSIAAYRSGLNINPLVKSQDAEDGLYKDLCARKPVRISNKSFIDYIFTLSLEVATSFDCPMQIHTGFGDKDLDLRLANPLHLRPILEDDRFKKSRIVLLHASYPYSKEASYLASVYPQIYLDCGLAIPKLSVHGMFSAVKDLLELCPLNKVMFSTDGYAFPETYYLAAKWARKVVVSVLSDACDGGDLSIQEAIEAAQNILRDNAVSFYKLNENSTQNVISEVGTSVTNSQILDLEGNEGKPKFVRLLWVDTSGQQRCRVVPENRFNKVVKYHGVGLTFACMGMTSSSDGPAEGSNLTGVGEIRLMPDMKTLCSLPWSDQEKMVLVDMHLKPSHPWEYCPRTALLRVTMTLEEEFGLVVNAGFESEFYILRKTTREVNNKWVPIDSTLYCSTVAFDAATPVLFEVIKALQKMHISVEQVHAESGNGQFEISLGHSPCMEAADNLVFVKETIRAIANRNGLLATFIPKYASNDVGSGCHVHLSLWQNGKNKFMAEDGSITKHGMSTIGEEFMAGVFHHLPSILAFTAPLPNSYERLKPNTWSGAYHCWGKENREAPIRTACPPGISSEVVSNFEIKAFDGCANPYLGLAAVIAAGIDGLRKHLVLPEPVDTNPDYLKEGAVHRLPTTLDESVAELRKNEVLKNLIGDKLMNAVIAVREAEIRYYSATLDNGVEKLIHRY*

Protein Length: 707

Fln nts: TGGTACAACATGGTCACTAGAGAGGTTCATTCAGCTGTTTCAGTCGACATTGAAATCACATGCCAAGGACGTTGTTGCCTTTAAGAGTATAGCTGCTTATCGCAGTGGTCTTAATATAAATCCATTGGTGAAAAGTCAAGATGCAGAAGATGGTCTTTACAAAGATTTATGCGCCAGGAAGCCTGTACGCATTAGTAACAAAAGCTTCATTGACTACATTTTTACTCTCAGCTTGGAAGTGGCCACATCTTTTGATTGTCCCATGCAAATACATACAGGTTTTGGAGACAAGGACCTGGACCTGAGGCTTGCAAACCCTCTTCATCTTCGGCCAATCTTGGAAGATGATCGATTCAAAAAATCTCGCATTGTGCTTCTTCATGCGTCCTATCCCTACTCAAAGGAAGCATCTTATTTGGCTTCTGTTTATCCACAGATCTACTTGGACTGTGGCCTAGCAATACCAAAGCTCAGTGTTCATGGGATGTTCTCTGCAGTTAAGGATCTGTTAGAACTGTGCCCACTAAACAAGGTGATGTTTAGCACAGATGGATATGCATTTCCCGAAACATATTATCTTGCTGCAAAATGGGCACGCAAAGTAGTTGTATCTGTTCTTTCTGATGCTTGTGATGGTGGAGATCTCTCTATTCAAGAAGCTATTGAAGCCGCACAGAATATCTTACGGGACAATGCAGTCAGTTTCTATAAGCTGAATGAAAATAGTACACAAAATGTGATATCTGAAGTGGGTACCTCAGTGACTAATTCTCAGATTCTGGATCTAGAAGGTAATGAAGGAAAACCTAAGTTTGTTCGTCTCCTTTGGGTTGATACTTCAGGTCAGCAAAGATGTCGAGTTGTTCCAGAAAATCGTTTCAACAAAGTAGTGAAGTATCATGGAGTGGGTTTAACATTTGCGTGCATGGGTATGACTTCAAGCTCAGATGGTCCAGCTGAAGGTTCCAATCTTACTGGTGTTGGTGAAATCCGACTGATGCCTGACATGAAGACACTGTGCAGTTTGCCCTGGTCTGACCAAGAGAAGATGGTATTAGTTGATATGCATTTGAAACCTAGCCATCCTTGGGAATATTGCCCAAGGACAGCCTTACTAAGAGTTACAATGACATTGGAAGAAGAATTTGGCTTGGTTGTGAATGCTGGTTTTGAGAGTGAGTTCTACATTTTGAGGAAAACAACGAGGGAAGTTAATAACAAGTGGGTGCCCATTGACTCGACCCTATACTGCTCAACTGTAGCATTTGATGCTGCAACTCCTGTTCTATTCGAAGTTATTAAAGCTCTCCAAAAGATGCACATATCTGTAGAACAGGTGCATGCAGAATCAGGGAATGGCCAGTTTGAAATATCCTTGGGGCATTCACCTTGCATGGAGGCAGCTGATAACTTAGTTTTCGTAAAAGAGACTATAAGGGCAATAGCAAACAGAAATGGACTTCTTGCGACGTTTATACCCAAGTATGCTTCAAATGACGTTGGTTCAGGATGCCATGTACATTTAAGTTTATGGCAAAACGGCAAGAACAAGTTTATGGCTGAAGATGGGTCAATTACTAAGCATGGTATGTCCACAATCGGGGAAGAGTTTATGGCTGGAGTCTTCCATCATCTTCCGTCCATATTGGCCTTCACAGCTCCATTGCCAAACAGTTATGAGCGATTGAAGCCTAACACATGGAGTGGAGCATATCATTGTTGGGGGAAGGAAAATCGAGAAGCTCCAATCCGAACAGCATGTCCTCCTGGGATTTCAAGTGAAGTCGTGAGCAACTTTGAGATTAAAGCATTTGATGGTTGTGCTAATCCATATCTGGGACTAGCAGCAGTTATAGCTGCTGGAATAGACGGCCTGCGGAAGCACCTTGTTTTGCCAGAGCCTGTTGACACAAACCCGGACTATCTCAAGGAAGGTGCGGTGCACAGATTGCCCACAACGTTGGATGAGTCTGTTGCAGAACTCAGAAAGAATGAAGTCCTGAAGAACCTTATTGGCGATAAACTCATGAATGCAGTAATTGCTGTCCGCGAGGCTGAAATTCGATATTATTCCGCCACCTTAGACAATGGTGTGGAGAAATTGATCCATAGGTATTGA___AGTTCAAATTTATGACAAAATGATGTGATAGCATTCTGGGAATTATAACATACTATATGTTATCTAACAAAGTGTGTGGTATTTTGAATTGTAATATTTTTGAAGACATCAAAGGGTACCTTATATGTTATCTAACAAAGTGTGTGGTATTTTGAATTGTTTTATTTTTGAAGACATCAAAGGGTACCTTAAATCACATCACTAACAGTTATTTCTTACCATTCTAATTATTGCACGTTATAATGAGTTTGTTAACGAGTATTGAGTGGTTAGTTAGCAATCCCATGGAAGATGCATGTAATTATTATATATTTTGTGAAAGTATTGCAAAATAAAGCGCTCGAGTAAGACTGGGATTGTTTTCAACATT

Fln Alignment:

Contig17___GTTWSLERFIQLFQSTLKSHAKDVVAFKSIAAYRSGLNINPLVKSQDAEDGLYKDLCARKPVRISNKSFIDYIFTLSLEVATSFDCPMQIHTGFGDKDLDLRLANPLHLRPILEDDRFKKSRIVLLHASYPYSKEASYLASVYPQIYLDCGLAIPKLSVHGMFSAVKDLLELCPLNKVMFSTDGYAFPETYYLAAKWARKVVVSVLSDACDGGDLSIQEAIEAAQNILRDNAVSFYKLN----ENSTQNVISEVGTSVTNSQILDLEGNEGKPKFVRLLWVDTSGQQRCRVVPENRFNKVVKYHGVGLTFACMGMTSSSDGPAEGSNLTGVGEIRLMPDMKTLCSLPWSDQEKMVLVDMHLKPSHPWEYCPRTALLRVTMTLEEEFGLVVNAGFESEFYILRKTTREVNNKWVPIDSTLYCSTVAFDAATPVLFEVIKALQKMHISVEQVHAESGNGQFEISLGHSPCMEAADNLVFVKETIRAIANRNGLLATFIPKYASNDVGSGCHVHLSLWQNGKNKFMAEDGSITKHGMSTIGEEFMAGVFHHLPSILAFTAPLPNSYERLKPNTWSGAYHCWGKENREAPIRTACPPGISSEVVSNFEIKAFDGCANPYLGLAAVIAAGIDGLRKHLVLPEPVDTNPDYLKEGAVHRLPTTLDESVAELRKNEVLKNLIGDKLMNAVIAVREAEIRYYSATLDNGVEKLIHRY
D7TIU8____________GSPWTLDVFTAIFVGKLKSYPLHI---SYIAAYRSGLEINTNVSRQDAEEGLAEVLYAGKPVRITNKNFIDYIFTRSLEVALCFDLPMQLHTGFGDRDLDLRLANPLHLRTLLEDKRFSKCRIVLLHASYPFSKEASYLASVYPQVYLDFGLAIPKLSTHGMISSVKELLELAPIKKVMFSTDGYAFPETFYLGAKKAREVVFTVLRDACIDGDLSIPEAVEAAEDIFAKNAIQFYKLNVAMKSIDLKNAICPKLMSIGNNS------SQNDITLVRIIWVDASGQQRCRVVPARRFYDVVLKNGVGLTFACMGMSSAIDGPADGTNLSGVGETRLVPDLSTKCRIPWAKQEEMVLADMHLKPGKPWEYCPREALRRISKVLKDEFNLVVNAGFEVEFYLLKRILREGKEEWVPFDSTPYCSTSAFDAASPIFHEVLAALQSLNVPVEQLHAEAGKGQFEIALGHTVCSCSADNLIFTHEVIKACARRHGLLATFVPKYTLDDIGSGSHVHISLWENGENVFMA-SGQGSHYGISKVGEEFMAGVLHHLPSILAFTAPVPNSYDRIQPDTWSGAYQCWGQENREAPLRTACPPGVPDGLVSNFEIKSFDGCANPHLGLASIIASGIDGLRKHLQLPVPVDENPSDL-SAELRRLPKSLSESLEALGKDNVMKDLIGEKLLVAIKGIRKAEINYYSQNVD-AYKQLIHRY

SNPs (tot: 38)

Position	A %	C %	G %	T %	Probability
836	10		90		0.90298
844	90			10	0.90298
869	10			90	0.90298
907			87	12	0.992554
953		16		83	0.994512
955			50	50	1.0
1157	16			83	0.994512
1158			16	83	0.994512
1159		83		16	0.994512
1160			16	83	0.994512
1165	83	16			0.994512
1166		16		83	0.994512
1168	16			83	0.994512
1170			16	83	0.994512
1171			83	16	0.994512
1177			16	83	0.994512
1178	16			83	0.994512
1179		16		83	0.994512
1264		83		16	0.994512
1495	14		85		0.993533
1497		85		14	0.993533
2108	87		12		0.992554
2158	10		90		0.90298
2159		90	10		0.90298
2160		90		10	0.90298
2165	90	10			0.90298
2166		10		90	0.90298
2171	90		10		0.90298
2172			90	10	0.90298
2173		10	90		0.90298
2175	11			88	0.923564
2177	11		11	77	0.968132
2203		87		12	0.992554
2271	83		16		0.994512
2307		12	87		0.992554
2308	12		87		0.992554
2309		87		12	0.992554
2437		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain