SustainPineDB

Assembly Name: inia v1.0

UniGene Name: inia_v1.0_unigene247

Length: 1473 nt

UniGene Fasta

Ace file of the UniGene inia_v1.0_unigene247

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene16052	33/33

Source	Descriptions	Term	Type	Identity
AutoFact	Fumarate hydratase 1, mitochondrial n=3 Tax=Arabidopsis RepID=FUM1_ARATH	-	-	79%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	fumarate class ii	-	-	91%

Source	ECs	Term	Type	e value	Identity
Blast2go	Fumarate hydratase.	EC:4.2.1.2	-	0.0	91%

Source	KEGGs	Term	Identity
Blast2go	Citrate cycle (TCA cycle)	00020	91%
Blast2go	Carbon fixation pathways in prokaryotes	00720	91%
Blast2go	Biosynthesis of phenylpropanoids	01061	91%
Blast2go	Biosynthesis of terpenoids and steroids	01062	91%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	91%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	91%
Blast2go	Biosynthesis of plant hormones	01070	91%
Blast2go	Metabolic pathways	01100	91%
Blast2go	Biosynthesis of secondary metabolites	01110	91%

Source	GOs	Term	Type	Identity
Blast2go	pollen tube development	GO:0048868	Biological Process	91%
Blast2go	response to oxidative stress	GO:0006979	Biological Process	91%
Blast2go	response to salt stress	GO:0009651	Biological Process	91%
Blast2go	nitrate assimilation	GO:0042128	Biological Process	91%
Blast2go	fumarate metabolic process	GO:0006106	Biological Process	91%
Blast2go	fumarate hydratase activity	GO:0004333	Molecular Function	91%
Blast2go	protein binding	GO:0005515	Molecular Function	91%
Blast2go	cytosol	GO:0005829	Cellular Component	91%
Blast2go	mitochondrion	GO:0005739	Cellular Component	91%

Source	InterPros	Term	Type	e value	Identity
Sma3	Fumarate hydratase, class II	IPR005677	-	0.0	-
Sma3	Fumarate lyase	IPR000362	-	0.0	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NVX1

Fln msg: Separated hits, possible frame ERROR between 902 and 917, Distance to subject end: 39 aas, your sequence is shorter than subject: 455 - 495

Fln protein: MAFIPAYRRHLSISTASSLMHGISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGKKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSEFIPSLRQLLSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNSLPRIYQLAQGGTAVGxxxxxxKGFDIKIAAAVAEETQLPFITAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCGLGELLLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDKAAAT

Protein Length: 456

Fln nts: GGATTCAACAAACTCTTGTTGGCCTAGTGGCGAAAGAAGATTAGGCACCTGGGCACAATCGAAGGGAGCTCCGGAACATTGCTTGGGACACTAGGTGGTGTGATATA_-_ATGGCATTCATTCCAGCATATCGACGCCATCTATCTATATCTACTGCATCTTCCTTGATGCATGGCATATCATTTACATACAGCAGATCACTTTCGACGGCTTTTAGAGAGGAGAGGGATACATTTGGTCCCATCAATGTACCGGCAGACAAATTATGGGGTGCACAGACTCAGCGGTCACTGCAAAACTTTGAAATTGGTGGAGAGCGTGAGCAGATGCCTGAACAAATTATACAATCATTTGGTGTTCTGAAAAAATGCGCTGCTAAGGTTAATATGGAGTATGGTCTTGACCCAACCATTGGGAAAGCGATAATGCAAGCTGCTCAAGAAGTTGCTGAAGGAAAGCTGAACAGTCATTTCCCTTTGGTCATTTGGCAAACTGGTAGTGGGACTCAAAGTAACATGAACGCTAACGAGGTGATTGCAAATCGTGCTGCTGAAATTCTTGGAAAGAAACGTGGTGAAAAATGTGTACATCCAAATGACCATGTCAATATGTCTCAATCTTCTAATGATACATTTCCCACTGTTATGCACATTGCTGCAGCAGTGGAAATGAACTCAGAATTCATACCAAGCTTACGGCAGCTTCTTTCTACATTGGAAGCTAAGAGCAATGAATTTAAGGATATAGTGAAAATTGGACGTACACATACCCAAGATGCAACACCGCTAACTCTTGGTCAAGAGTTTAGTGGTTATGCTACCCAGGTGAAATACGGCATAGAACGTGTTACAAATTCATTGCCTCGTATATACCAGCTTGCACAAGGAGGGACTGCAGTTGGAACxxxxxxxxxxxxxxxAAAAGGGTTTGACATTAAAATTGCTGCAGCTGTTGCTGAAGAAACACAGCTACCATTTATTACAGCTGAGAACAAGTTTGAAGCTTTGGCAGCACATGATGCTTTTGTTGAAACCAGTGGAGCTGTGAACACTGTTTCAGTTTCTCTAATGAAGATAGCAAATGATATACGTTTCCTAGGAAGTGGGCCTCGCTGTGGACTAGGTGAACTGCTATTACCTGAAAACGAACCTGGCAGCAGCATCATGCCTGGAAAGGTGAACCCTACTCAATGTGAGGCTTTGACAATGGTTTGTGCTCAGGTTATGGGAAACCATGTTGCAATTACAGTAGGGGGATCAAATGGTCATTTTGAGCTTAATGTTTTTAAGCCATTGATCGCAAGTGCTCTACTTCGGTCTTTGACATTGCTAGGAGATGCTGCAGCTTCATTTGAAAAGAATTGTGTAAGGGGAATTCAGGCCAACCATCGAAGAATAGGACAGCTTGTCCGAGAGTCACTGATGCTTGTTACAGCTCTGAATCCTAAAATTGGGTATGACAAAGCAGCAGCAACTGC

Fln Alignment:

Contig247___MAFIPAYRRHLSISTASSLMHGISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGKKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSEFIPSLRQLLSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNSLPRIYQLAQGGTAVGTxxxxxKGFDIKIAAAVAEETQLPFITAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCXXXXXXXXXXXXXSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDKAAAT
A9NVX1_______________MAIISAYRRHLSISTASSLMHNISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSKFIPSLRQLHSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNTLPRIYQLAQGGTAVGTxxxxxKGFDIKIAAAVAEETQLPFLTAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCGLGELLLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDKAAAT

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
732		11		88	0.923564
776	88		11		0.923564
869		90	10		0.90298
871	90			10	0.90298
875		11		88	0.923564
1008	16		83		0.994512
1082		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain